| Code name | IE_06H08 | |||
|---|---|---|---|---|
| Locus | AT5G54360 | |||
| Template | IT_06H08 (ABRC_213) | |||
| Donor Vector | pDONR207 | |||
| Forward primer | IPF_06H08 (ggggacaagtttgtacaaaaaagcaggcttcATGAACAACCAATCTATGTCTTCTTCA) | |||
| Reverse primer | IPR_06H08 (ggggaccactttgtacaagaaagctgggttCTCTCCTCTTGATCTTTTTTTCTTGGGCAA) | |||
| Alignment with template seq. | 1st | ã¢ããé ¸é å85.90%ä¸è´ | 2nd | ã¢ããé ¸é å85.90%ä¸è´ |
| Alignment with TAIR7CDS | ã¢ããé ¸é å85.90%ä¸è´ | ã¢ããé ¸é å85.90%ä¸è´ | ||
| comment | 1st:(Sequence解æã¾ã§è¡ã£ãããèªã¿ç´ãå¿ è¦ããã?), 2nd:70%, nt.170T→G, nt.209T→C, nt.368A→T, nt.442T→C, nt.625C→T, nt.628G→A, nt.634G→C, nt.637T→A, nt.641C→A, 46塩基が13塩基に置換, 23塩基が6塩基に置換 | |||
| sequence |
>IE_06H08_760bp ATGAACAACCAATCTATGTCTTCTTCAAGCTCCTCCTCCCCACACAAACATGATCAAAAACTCAAAAGTGCCCTTGTGGCCGTGGAGGAGAAGGAGACAA TAAACAATCCGAAACAAAGGTCTCCTCAAGAGATTGTGTCTGAAAGGACAAACCTTGAGATGCCTAGTTGCAGATTAAGTTTTTTCCGAAAGAAAAGAGA TACCGTGCCAATACATCCTCCCAAGGATAACAAGATCAGCCACACCTGTCGTTTCTGCAAGAGGAATTTCAAAAGCTGTTTTGCCCTAGGCGGTCACATG AAGTGCCACAAAAAGGAACGAGAATTGGAGAAGCAGCGAAAGATAATCGAGGATGCGATGCTTTGTGTTAGTACTCCTTTCACCTTGCAAAGGCTTCCTG TTGGCCCTTGTAAGTATGGCCAAGGTGGTAGCTCACATGCTCTATCAACTGATGGTATTAATCTTGATCTAACTCTTGGTCCATCCAAGTCCACAGGAGG CAGCAACAATAGCACTAATAACAACACTAACTCATCGTTCCATGGGAATCTAATGACTCCCGTGGGTCCTTGTGTGCCTAGATACAGTTTGGTTGCTGGA AACCAGGTTGATTCAAGCAATATATATATTCCTCCTAATCAAACCACCGATCTTTGTTATGATTTTTTTGCTTTACAAGAGGGATCTAAGGGGGAAAACA AAGTCATGGTGCCTGAAGATGATAAGGTCGTTGCCCAAGAAAAAAAGATCAAGAGGAGAG >IE_06H08_translated MNNQSMSSSSSSSPHKHDQKLKSALVAVEEKETINNPKQRSPQEIVSERTNLEMPSCRLSFFRKKRDTVPIHPPKDNKISHTCRFCKRNFKSCFALGGHM KCHKKERELEKQRKIIEDAMLCVSTPFTLQRLPVGPCKYGQGGSSHALSTDGINLDLTLGPSKSTGGSNNSTNNNTNSSFHGNLMTPVGPCVPRYSLVAG NQVDSSNIYIPPNQTTDLCYDFFALQEGSKGENKVMVPEDDKVVAQEKKIKRRX |
|||
| Alignment |
◆CDS（TAIRver.9）_without_stop_codonとのalignment◆ 748/810 (92.3%)（アミノ酸配列で231/270 (85.6%)）一致しました。
10 20 30 40 50 60 70 80 90 100
AT5G54 ATGAACAACCAATCTATGTCTTCTTCAAGCTCCTCCTCCCCACACAAACATGATCAAAAACTCAAAAGTGCCCTTGTGGCCGTGGAGGAGAAGGAGACAA
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_06H ATGAACAACCAATCTATGTCTTCTTCAAGCTCCTCCTCCCCACACAAACATGATCAAAAACTCAAAAGTGCCCTTGTGGCCGTGGAGGAGAAGGAGACAA
10 20 30 40 50 60 70 80 90 100
110 120 130 140 150 160 170 180 190 200
AT5G54 TAAACAATCCGAAACAAAGGTCTCCTCAAGAGATTGTGTCTGAAAGGACAAACCTTGAGATGCCTAGTTTCAGATTAAGTTTTTTCCGAAAGAAAAGAGA
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: ::::::::::::::::::::::::::::::
IE_06H TAAACAATCCGAAACAAAGGTCTCCTCAAGAGATTGTGTCTGAAAGGACAAACCTTGAGATGCCTAGTTGCAGATTAAGTTTTTTCCGAAAGAAAAGAGA
110 120 130 140 150 160 170 180 190 200
210 220 230 240 250 260 270 280 290 300
AT5G54 TACCGTGCTAATACATCCTCCCAAGGATAACAAGATCAGCCACACCTGTCGTTTCTGCAAGAGGAATTTCAAAAGCTGTTTTGCCCTAGGCGGTCACATG
:::::::: :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_06H TACCGTGCCAATACATCCTCCCAAGGATAACAAGATCAGCCACACCTGTCGTTTCTGCAAGAGGAATTTCAAAAGCTGTTTTGCCCTAGGCGGTCACATG
210 220 230 240 250 260 270 280 290 300
310 320 330 340 350 360 370 380 390 400
AT5G54 AAGTGCCACAAAAAGGAACGAGAATTGGAGAAGCAGCGAAAGATAATCGAGGATGCGATGCTTTGTGATAGTACTCCTTTCACCTTGCAAAGGCTTCCTG
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: ::::::::::::::::::::::::::::::::
IE_06H AAGTGCCACAAAAAGGAACGAGAATTGGAGAAGCAGCGAAAGATAATCGAGGATGCGATGCTTTGTGTTAGTACTCCTTTCACCTTGCAAAGGCTTCCTG
310 320 330 340 350 360 370 380 390 400
410 420 430 440 450 460 470 480 490 500
AT5G54 TTGGCCCTTGTAAGTATGGCCAAGGTGGTAGCTCACATGCTTTATCAACTGATGGTATTAATCTTGATCTAACTCTTGGTCCATCCAAGTCCACAGGAGG
::::::::::::::::::::::::::::::::::::::::: ::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_06H TTGGCCCTTGTAAGTATGGCCAAGGTGGTAGCTCACATGCTCTATCAACTGATGGTATTAATCTTGATCTAACTCTTGGTCCATCCAAGTCCACAGGAGG
410 420 430 440 450 460 470 480 490 500
510 520 530 540 550 560 570 580 590 600
AT5G54 CAGCAACAATAGCACTAATAACAACACTAACTCATCGTTCCATGGGAATCTAATGACTCCCGTGGGTCCTTGTGTGCCTAGATACAGTTTGGTTGCTGGA
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_06H CAGCAACAATAGCACTAATAACAACACTAACTCATCGTTCCATGGGAATCTAATGACTCCCGTGGGTCCTTGTGTGCCTAGATACAGTTTGGTTGCTGGA
510 520 530 540 550 560 570 580 590 600
610 620 630 640 650 660 670 680 690 700
AT5G54 AACCAGGTTGATTCAAGCAATATACATGTTCCTGCTTATCCAACCACCGATCTTTGTTATGATTTTTTTGCTTTACAAGAGCATGGTTCGGGCTCTAGTC
:::::::::::::::::::::::: :: ::::: :: ::: ::::::::::::::::::::::::::::::::::::::: ::: :::
IE_06H AACCAGGTTGATTCAAGCAATATATATATTCCTCCTAATCAAACCACCGATCTTTGTTATGATTTTTTTGCTTTACAAGA---------GGGATCT----
610 620 630 640 650 660 670 680
710 720 730 740 750 760 770 780 790 800
AT5G54 ACTCAAAAAGTCTCATCTCAAAGGGGAAAAACAAAGTCATGGTGCCTGAAGATGATGAGGATATCGGGATGATAAGGTGGTTGCCCAAGAAAAAAAGATC
:::::: ::::::::::::::::::::::: ::::::::::: :::::::::::::::::::::
IE_06H --------------------AAGGGGGAAAACAAAGTCATGGTGCCTGAA-----------------GATGATAAGGTCGTTGCCCAAGAAAAAAAGATC
690 700 710 720 730 740 750
810
AT5G54 AAGAGGAGAG
::::::::::
IE_06H AAGAGGAGAG
760
10 20 30 40 50 60 70 80 90 100
AT5G54 MNNQSMSSSSSSSPHKHDQKLKSALVAVEEKETINNPKQRSPQEIVSERTNLEMPSFRLSFFRKKRDTVLIHPPKDNKISHTCRFCKRNFKSCFALGGHM
:::::::::::::::::::::::::::::::::::::::::::::::::::::::: :::::::::::: ::::::::::::::::::::::::::::::
IE_06H MNNQSMSSSSSSSPHKHDQKLKSALVAVEEKETINNPKQRSPQEIVSERTNLEMPSCRLSFFRKKRDTVPIHPPKDNKISHTCRFCKRNFKSCFALGGHM
10 20 30 40 50 60 70 80 90 100
110 120 130 140 150 160 170 180 190 200
AT5G54 KCHKKERELEKQRKIIEDAMLCDSTPFTLQRLPVGPCKYGQGGSSHALSTDGINLDLTLGPSKSTGGSNNSTNNNTNSSFHGNLMTPVGPCVPRYSLVAG
:::::::::::::::::::::: :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_06H KCHKKERELEKQRKIIEDAMLCVSTPFTLQRLPVGPCKYGQGGSSHALSTDGINLDLTLGPSKSTGGSNNSTNNNTNSSFHGNLMTPVGPCVPRYSLVAG
110 120 130 140 150 160 170 180 190 200
210 220 230 240 250 260 270
AT5G54 NQVDSSNIHVPAYPTTDLCYDFFALQEHGSGSSHSKSLISKGKNKVMVPEDDEDIGMIRWLPKKKRSRGE
::::::::..: ::::::::::::: :::.:::::::::. . . . ..
IE_06H NQVDSSNIYIPPNQTTDLCYDFFALQEG-----------SKGENKVMVPEDDKVVAQEKKIKRRX-----
210 220 230 240 250
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