◆◆◆IE_07A01 detail◆◆◆

Code nameIE_07A01
LocusAT2G26960
TemplateIT_07A01 (ABRC_003)
Donor VectorpDONR207
Forward primerIPF_07A01 (ggggacaagtttgtacaaaaaagcaggcttcATGGGGAAAGTTCGTCAAGATTCAGGA)
Reverse primerIPR_07A01 (ggggaccactttgtacaagaaagctgggttAAGGATGTGTGTCCCTGATTTGAAGGAGTT)
Alignment with template seq.1st配列解析が行えなかったサンプルです。2ndアミノ酸配列100%一致
Alignment with TAIR7CDS-アミノ酸配列100%一致
comment1st:(Sequence解析まで行ったが、読み直す必要がある), 2nd:70%, nt.852G→A
sequence 
>IE_07A01_1281bp
ATGGGGAAAGTTCGTCAAGATTCAGGATCAGACGATGACAATTCAATAAAGAAATCTTTTACTAAAGGTCCTTGGACACAAGCAGAAGACAATCTTTTGA
TAGCTTACGTTGATAAACATGGTGATGGAAACTGGAACGCTGTTCAAAACAACTCGGGCCTTTCTCGTTGTGGTAAAAGTTGTCGCCTTCGTTGGGTAAA
TCATCTGAGACCAGATTTGAAAAAAGGCGCTTTTACAGAGAAAGAAGAGAAACGTGTCATCGAACTTCATGCTTTGTTAGGGAACAAATGGGCACGAATG
GCTGAAGAACTACCGGGACGAACAGATAATGAGATCAAGAACTTCTGGAATACAAGACTTAAGAGACTGCAACGACTTGGTTTACCTGTTTACCCCGATG
AAGTTCGAGAGCATGCGATGAATGCAGCGACACATTCCGGTCTAAACACTGATTCATTGGATGGTCATCATAGTCAAGAATATATGGAGGCAGATACTGT
TGAGATTCCTGAAGTGGATTTCGAGCATTTACCACTCAATCGATCTTCTTCATACTACCAGTCAATGCTTCGACATGTTCCTCCCACTAACGTCTTTGTG
AGACAAAAACCGTGTTTCTTTCAGCCGCCTAATGTGTATAACTTGATACCACCATCTCCTTACATGTCTACCGGAAAACGTCCTAGAGAACCAGAAACCG
CGTTTCCTTGCCCGGGTGGATATACCATGAACGAGCAAAGCCCTCGGCTCTGGAACTATCCTTTTGTTGAGAACGTTTCAGAACAGTTACCGGATAGTCA
TTTGCTTGGTAATGCTGCTTATTCTTCTCCTCCTGGACCTCTTGTTCACGGAGTTGAGAATTTCGAGTTCCCTTCATTCCAATATCATGAAGAGCCTGGC
GGTTGGGGAGCAGATCAACCTAACCCAATGCCGGAACATGAGTCAGATAACACTTTGGTTCAATCTCCTCTGACAGCTCAAACACCATCAGATTGTCCAT
CATCATCACTCTATGATGGACTGTTGGAATCAGTTGTCTATGGATCATCAGGGGAGAAACCGGCAACCGATACCGATTCAGAATCTTCACTGTTTCAGTC
ATTTACACCAGCCAATGAAAACATAACCGGTAAAACTTGTTTTCTTACTCTTTATGCATTACATGCACTTCATTGTTTGTGTAATCAATTCAAGAAGTCT
CCTCTTCTCCATCTGCATGACAAACTTAATTGGTGTAACAAATTTAGGTTCAACTCCTTCAAATCAGGGACACACATCCTT

>IE_07A01_translated
MGKVRQDSGSDDDNSIKKSFTKGPWTQAEDNLLIAYVDKHGDGNWNAVQNNSGLSRCGKSCRLRWVNHLRPDLKKGAFTEKEEKRVIELHALLGNKWARM
AEELPGRTDNEIKNFWNTRLKRLQRLGLPVYPDEVREHAMNAATHSGLNTDSLDGHHSQEYMEADTVEIPEVDFEHLPLNRSSSYYQSMLRHVPPTNVFV
RQKPCFFQPPNVYNLIPPSPYMSTGKRPREPETAFPCPGGYTMNEQSPRLWNYPFVENVSEQLPDSHLLGNAAYSSPPGPLVHGVENFEFPSFQYHEEPG
GWGADQPNPMPEHESDNTLVQSPLTAQTPSDCPSSSLYDGLLESVVYGSSGEKPATDTDSESSLFQSFTPANENITGKTCFLTLYALHALHCLCNQFKKS
PLLHLHDKLNWCNKFRFNSFKSGTHIL
Alignment ◆CDS（TAIRver.9）_without_stop_codonとのalignment◆

1280/1281 (99.9%)（アミノ酸配列で100%）一致しました。


               10        20        30        40        50        60        70        80        90       100
AT2G26 ATGGGGAAAGTTCGTCAAGATTCAGGATCAGACGATGACAATTCAATAAAGAAATCTTTTACTAAAGGTCCTTGGACACAAGCAGAAGACAATCTTTTGA
       ::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_07A ATGGGGAAAGTTCGTCAAGATTCAGGATCAGACGATGACAATTCAATAAAGAAATCTTTTACTAAAGGTCCTTGGACACAAGCAGAAGACAATCTTTTGA
               10        20        30        40        50        60        70        80        90       100

              110       120       130       140       150       160       170       180       190       200
AT2G26 TAGCTTACGTTGATAAACATGGTGATGGAAACTGGAACGCTGTTCAAAACAACTCGGGCCTTTCTCGTTGTGGTAAAAGTTGTCGCCTTCGTTGGGTAAA
       ::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_07A TAGCTTACGTTGATAAACATGGTGATGGAAACTGGAACGCTGTTCAAAACAACTCGGGCCTTTCTCGTTGTGGTAAAAGTTGTCGCCTTCGTTGGGTAAA
              110       120       130       140       150       160       170       180       190       200

              210       220       230       240       250       260       270       280       290       300
AT2G26 TCATCTGAGACCAGATTTGAAAAAAGGCGCTTTTACAGAGAAAGAAGAGAAACGTGTCATCGAACTTCATGCTTTGTTAGGGAACAAATGGGCACGAATG
       ::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_07A TCATCTGAGACCAGATTTGAAAAAAGGCGCTTTTACAGAGAAAGAAGAGAAACGTGTCATCGAACTTCATGCTTTGTTAGGGAACAAATGGGCACGAATG
              210       220       230       240       250       260       270       280       290       300

              310       320       330       340       350       360       370       380       390       400
AT2G26 GCTGAAGAACTACCGGGACGAACAGATAATGAGATCAAGAACTTCTGGAATACAAGACTTAAGAGACTGCAACGACTTGGTTTACCTGTTTACCCCGATG
       ::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_07A GCTGAAGAACTACCGGGACGAACAGATAATGAGATCAAGAACTTCTGGAATACAAGACTTAAGAGACTGCAACGACTTGGTTTACCTGTTTACCCCGATG
              310       320       330       340       350       360       370       380       390       400

              410       420       430       440       450       460       470       480       490       500
AT2G26 AAGTTCGAGAGCATGCGATGAATGCAGCGACACATTCCGGTCTAAACACTGATTCATTGGATGGTCATCATAGTCAAGAATATATGGAGGCAGATACTGT
       ::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_07A AAGTTCGAGAGCATGCGATGAATGCAGCGACACATTCCGGTCTAAACACTGATTCATTGGATGGTCATCATAGTCAAGAATATATGGAGGCAGATACTGT
              410       420       430       440       450       460       470       480       490       500

              510       520       530       540       550       560       570       580       590       600
AT2G26 TGAGATTCCTGAAGTGGATTTCGAGCATTTACCACTCAATCGATCTTCTTCATACTACCAGTCAATGCTTCGACATGTTCCTCCCACTAACGTCTTTGTG
       ::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_07A TGAGATTCCTGAAGTGGATTTCGAGCATTTACCACTCAATCGATCTTCTTCATACTACCAGTCAATGCTTCGACATGTTCCTCCCACTAACGTCTTTGTG
              510       520       530       540       550       560       570       580       590       600

              610       620       630       640       650       660       670       680       690       700
AT2G26 AGACAAAAACCGTGTTTCTTTCAGCCGCCTAATGTGTATAACTTGATACCACCATCTCCTTACATGTCTACCGGAAAACGTCCTAGAGAACCAGAAACCG
       ::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_07A AGACAAAAACCGTGTTTCTTTCAGCCGCCTAATGTGTATAACTTGATACCACCATCTCCTTACATGTCTACCGGAAAACGTCCTAGAGAACCAGAAACCG
              610       620       630       640       650       660       670       680       690       700

              710       720       730       740       750       760       770       780       790       800
AT2G26 CGTTTCCTTGCCCGGGTGGATATACCATGAACGAGCAAAGCCCTCGGCTCTGGAACTATCCTTTTGTTGAGAACGTTTCAGAACAGTTACCGGATAGTCA
       ::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_07A CGTTTCCTTGCCCGGGTGGATATACCATGAACGAGCAAAGCCCTCGGCTCTGGAACTATCCTTTTGTTGAGAACGTTTCAGAACAGTTACCGGATAGTCA
              710       720       730       740       750       760       770       780       790       800

              810       820       830       840       850       860       870       880       890       900
AT2G26 TTTGCTTGGTAATGCTGCTTATTCTTCTCCTCCTGGACCTCTTGTTCACGGGGTTGAGAATTTCGAGTTCCCTTCATTCCAATATCATGAAGAGCCTGGC
       ::::::::::::::::::::::::::::::::::::::::::::::::::: ::::::::::::::::::::::::::::::::::::::::::::::::
IE_07A TTTGCTTGGTAATGCTGCTTATTCTTCTCCTCCTGGACCTCTTGTTCACGGAGTTGAGAATTTCGAGTTCCCTTCATTCCAATATCATGAAGAGCCTGGC
              810       820       830       840       850       860       870       880       890       900

              910       920       930       940       950       960       970       980       990      1000
AT2G26 GGTTGGGGAGCAGATCAACCTAACCCAATGCCGGAACATGAGTCAGATAACACTTTGGTTCAATCTCCTCTGACAGCTCAAACACCATCAGATTGTCCAT
       ::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_07A GGTTGGGGAGCAGATCAACCTAACCCAATGCCGGAACATGAGTCAGATAACACTTTGGTTCAATCTCCTCTGACAGCTCAAACACCATCAGATTGTCCAT
              910       920       930       940       950       960       970       980       990      1000

             1010      1020      1030      1040      1050      1060      1070      1080      1090      1100
AT2G26 CATCATCACTCTATGATGGACTGTTGGAATCAGTTGTCTATGGATCATCAGGGGAGAAACCGGCAACCGATACCGATTCAGAATCTTCACTGTTTCAGTC
       ::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_07A CATCATCACTCTATGATGGACTGTTGGAATCAGTTGTCTATGGATCATCAGGGGAGAAACCGGCAACCGATACCGATTCAGAATCTTCACTGTTTCAGTC
             1010      1020      1030      1040      1050      1060      1070      1080      1090      1100

             1110      1120      1130      1140      1150      1160      1170      1180      1190      1200
AT2G26 ATTTACACCAGCCAATGAAAACATAACCGGTAAAACTTGTTTTCTTACTCTTTATGCATTACATGCACTTCATTGTTTGTGTAATCAATTCAAGAAGTCT
       ::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_07A ATTTACACCAGCCAATGAAAACATAACCGGTAAAACTTGTTTTCTTACTCTTTATGCATTACATGCACTTCATTGTTTGTGTAATCAATTCAAGAAGTCT
             1110      1120      1130      1140      1150      1160      1170      1180      1190      1200

             1210      1220      1230      1240      1250      1260      1270      1280 
AT2G26 CCTCTTCTCCATCTGCATGACAAACTTAATTGGTGTAACAAATTTAGGTTCAACTCCTTCAAATCAGGGACACACATCCTT
       :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_07A CCTCTTCTCCATCTGCATGACAAACTTAATTGGTGTAACAAATTTAGGTTCAACTCCTTCAAATCAGGGACACACATCCTT
             1210      1220      1230      1240      1250      1260      1270      1280 




               10        20        30        40        50        60        70        80        90       100
AT2G26 MGKVRQDSGSDDDNSIKKSFTKGPWTQAEDNLLIAYVDKHGDGNWNAVQNNSGLSRCGKSCRLRWVNHLRPDLKKGAFTEKEEKRVIELHALLGNKWARM
       ::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_07A MGKVRQDSGSDDDNSIKKSFTKGPWTQAEDNLLIAYVDKHGDGNWNAVQNNSGLSRCGKSCRLRWVNHLRPDLKKGAFTEKEEKRVIELHALLGNKWARM
               10        20        30        40        50        60        70        80        90       100

              110       120       130       140       150       160       170       180       190       200
AT2G26 AEELPGRTDNEIKNFWNTRLKRLQRLGLPVYPDEVREHAMNAATHSGLNTDSLDGHHSQEYMEADTVEIPEVDFEHLPLNRSSSYYQSMLRHVPPTNVFV
       ::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_07A AEELPGRTDNEIKNFWNTRLKRLQRLGLPVYPDEVREHAMNAATHSGLNTDSLDGHHSQEYMEADTVEIPEVDFEHLPLNRSSSYYQSMLRHVPPTNVFV
              110       120       130       140       150       160       170       180       190       200

              210       220       230       240       250       260       270       280       290       300
AT2G26 RQKPCFFQPPNVYNLIPPSPYMSTGKRPREPETAFPCPGGYTMNEQSPRLWNYPFVENVSEQLPDSHLLGNAAYSSPPGPLVHGVENFEFPSFQYHEEPG
       ::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_07A RQKPCFFQPPNVYNLIPPSPYMSTGKRPREPETAFPCPGGYTMNEQSPRLWNYPFVENVSEQLPDSHLLGNAAYSSPPGPLVHGVENFEFPSFQYHEEPG
              210       220       230       240       250       260       270       280       290       300

              310       320       330       340       350       360       370       380       390       400
AT2G26 GWGADQPNPMPEHESDNTLVQSPLTAQTPSDCPSSSLYDGLLESVVYGSSGEKPATDTDSESSLFQSFTPANENITGKTCFLTLYALHALHCLCNQFKKS
       ::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_07A GWGADQPNPMPEHESDNTLVQSPLTAQTPSDCPSSSLYDGLLESVVYGSSGEKPATDTDSESSLFQSFTPANENITGKTCFLTLYALHALHCLCNQFKKS
              310       320       330       340       350       360       370       380       390       400

              410       420       
AT2G26 PLLHLHDKLNWCNKFRFNSFKSGTHIL
       :::::::::::::::::::::::::::
IE_07A PLLHLHDKLNWCNKFRFNSFKSGTHIL
              410       420

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