| Code name | IE_07A05 | |||
|---|---|---|---|---|
| Locus | AT5G58850 | |||
| Template | IT_07A05 (ABRC_009) | |||
| Donor Vector | pDONR207 | |||
| Forward primer | IPF_07A05 (ggggacaagtttgtacaaaaaagcaggcttcATGGAAGACCGACGCCTTGTCCATGGA) | |||
| Reverse primer | IPR_07A05 (ggggaccactttgtacaagaaagctgggttGACCAACGGGAAGCAGATGTTGCTACCTTG) | |||
| Alignment with template seq. | 1st | 配列解析が行えなかったサンプルです。 | 2nd | アミノ酸配列100%一致 |
| Alignment with TAIR7CDS | - | アミノ酸配列100%一致 | ||
| comment | 1st:(Sequence解析まで行ったが、読み直す必要がある) | |||
| sequence |
>IE_07A05_1290bp ATGGAAGACCGACGCCTTGTCCATGGAGCTGCGCCGCCGTTGACAGCCGTGGAGAGATTCTTATACGGTCAAAAGAATGATGCCTTGTGTTCCAAGAAAC AAGAGAGTTCTAGAGACCAACCGATCGTGAAGACAAAAATATCGATTGAGACTAGAAGCGACAACAAAGAGAACACAACGTTTGGGCCGACGAGGGAGAA ACACTTGGTCCTTAATGGCGGCAACAGAAACCCTACTGGAGAAGTGGTCGCTAGAAGTGCAGCCAGAGATTATCAAAACAGTACGAAGAAACGATCATCC AAGAACCTCATCAAAGGGCAATGGACGGCGGAAGAAGACAGGAAGTTGATAAGGTTGGTGAGGCAACATGGGGAGAGGAAATGGGCAATGATATCGGAGA AACTTGAGGGAAGAGCAGGCAAACAATGTCGTGAGAGATGGCATAATCATCTCCGTCCTGATATTAAGAAAGATGGATGGAGTGAAGAAGAAGAGAGGGT TCTTGTGGAATCACACATGAGAATCGGGAACAAATGGGCAGAGATCGCTAAACTCATACCAGGACGGACTGAGAATTCTATCAAGAACCATTGGAATGCG ACCAAGAGACGTCAAAATTCAAAACGCAAACACAAACGCGAATCAAACGCTGATAACAATGATAGAGATGCCTCTCCATCAGCTAAAAGACCATGCATTC TCCAAGACTACATCAAAAGTATCGAGAGAAATAACATTAACAAGGACAATGACGAGAAGAAGAATGAGAACACCATCAGTGTTATTTCTACACCTAATCT TGATCAGATCTATTCTGATGGTGATTCCGCGTCGTCCATCCTTGGCGGTCCATATGACGAAGAGCTAGATTACTTTCAAAATATCTTTGCAAACCATCCG ATTTCTCTTGAGAATTTAGGTTTGAGCCAAACTTCGGATGAGGTAACTCAGTCTTCATCATCTGGGTTCATGATCAAGAACCCTAACCCTAACTTGCACG ACAGTGTTGGAATTCATCATCAAGAGGCAACGATCACAGCTCCGGCGAATACACCCCACCTTGCGTCTGATATCTACTTATCTTATTTATTGAACGGTAC GACGTCTTCATACTCCGACACTCATTTTCCGTCTTCCTCATCTTCCACGTCATCCACCACCGTAGAGCATGGAGGCCACAACGAGTTTCTTGAGCCTCAA GCTAACTCTACAAGTGAAAGAAGAGAAATGGATCTGATAGAGATGCTCTCTGGTTCTATTCAAGGTAGCAACATCTGCTTCCCGTTGGTC >IE_07A05_translated MEDRRLVHGAAPPLTAVERFLYGQKNDALCSKKQESSRDQPIVKTKISIETRSDNKENTTFGPTREKHLVLNGGNRNPTGEVVARSAARDYQNSTKKRSS KNLIKGQWTAEEDRKLIRLVRQHGERKWAMISEKLEGRAGKQCRERWHNHLRPDIKKDGWSEEEERVLVESHMRIGNKWAEIAKLIPGRTENSIKNHWNA TKRRQNSKRKHKRESNADNNDRDASPSAKRPCILQDYIKSIERNNINKDNDEKKNENTISVISTPNLDQIYSDGDSASSILGGPYDEELDYFQNIFANHP ISLENLGLSQTSDEVTQSSSSGFMIKNPNPNLHDSVGIHHQEATITAPANTPHLASDIYLSYLLNGTTSSYSDTHFPSSSSSTSSTTVEHGGHNEFLEPQ ANSTSERREMDLIEMLSGSIQGSNICFPLV |
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| Alignment |
◆CDS（TAIRver.9）_without_stop_codonとのalignment◆ 100%一致しました。 |
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