| Code name | IE_07B01 | |||
|---|---|---|---|---|
| Locus | AT3G49530 | |||
| Template | IT_07B01 (ABRC_038) | |||
| Donor Vector | pDONR207 | |||
| Forward primer | IPF_07B01 (ggggacaagtttgtacaaaaaagcaggcttcATGAATCAGAATCTTCATGTATTATCA) | |||
| Reverse primer | IPR_07B01 (ggggaccactttgtacaagaaagctgggttGGACACTGCAGATGCTCTGAAGTCGACGGT) | |||
| Alignment with template seq. | 1st | é å解æãè¡ããªãã£ããµã³ãã«ã§ãã | 2nd | ã¢ããé ¸é å100%ä¸è´ |
| Alignment with TAIR7CDS | - | ã¢ããé ¸é å100%ä¸è´ | ||
| comment | 1st:(Sequence解æã¾ã§è¡ã£ãããèªã¿ç´ãå¿ è¦ããã), 2nd:70%, nt.870T→C | |||
| sequence |
>IE_07B01_1407bp ATGAATCAGAATCTTCATGTATTATCAATGGATTCGTTACCAGTTGGATTAAGATTCCGTCCAACAGACGAGGAGCTAATCCGTTACTATCTCCGTAGGA AAATCAACGGTCACGATGACGACGTCAAAGCTATCCGTGAGATCGATATTTGCAAATGGGAACCTTGGGATTTACCTGATTTTTCTGTGATCAAAACTAA AGACTCAGAGTGGCTCTACTTCTGTCCATTGGATCGGAAGTATCCGAGTGGAAGTAGACAGAACCGGGCAACAGTTGCAGGGTACTGGAAAGCCACAGGA AAAGACCGGAAGATAAAATCCGGTAAGACTAACATTATTGGTGTGAAGAGAACTCTAGTTTTCCACGCGGGTAGAGCTCCTAGGGGGACACGAACCAATT GGATTATTCATGAGTATCGTGCCACGGAGGATGATCTTAGTGGTACCAATCCTGGCCAGAGTCCGTTTGTTATATGCAAATTGTTCAAGAAAGAAGAACT GGTTTTAGGGGAAGAAGATTCGAAGTCAGATGAAGTTGAAGAACCTGCTGTCTCGTCTCCAACTGTCGAAGTGACTAAGTCAGAAGTATCTGAGGTAATT AAAACAGAAGACGTGAAGCGTCATGACATAGCAGAATCTTCTCTTGTAATCTCTGGAGATTCTCATAGTGATGCTTGTGATGAGGCTACAACCGCAGAGC TTGTAGATTTTAAATGGTATCCGGAATTGGAGTCCTTAGATTTCACGCTGTTCTCTCCATTACACTCTCAAGTCCAATCTGAGCTTGGATCCTCTTACAA CACATTCCAGCCTGGCTCGAGTAATTTTTCAGGGAACAACAACAACAGCTTCCAAATCCAGACTCAGTACGGTACAAATGAAGTAGATACGTATATATCT GATTTTCTTGATTCGATTCTCAAGAGCCCAGACGAGGATCCAGAGAAGCACAAGTATGTTTTGCAAAGTGGTTTTGATGTTGTAGCACCAGATCAGATTG CGCAAGTGTGTCAACAGGGCTCAGCAGTTGACATGAGCAATGATGTTTCAGTGACGGGTATCCAGATTAAGAGTCGCCAGGCACAACCCTCAGGTTATAC CAATGACTATATAGCGCAGGGAAATGGTCCAAGAAGGCTGCGTCTGCAAAGTAACTTTAATGGCATTAACACGAAGAATCCTGAGCTTCAAGCTATAAAG AGAGAGGCTGAGGACACGGTTGGAGAAAGTATAAAGAAAAGGTGTGGAAAGCTAATGAGATCAAAGAACGTGACCGGTTTTGTGTTTAAAAAGATTACGT CGGTGAAATGTTCATATGGAGGACTCTTTAGGGCGGCAGTAGTTGCAGTTGTGTTCTTGATGTCAGTCTGCAGTCTAACCGTCGACTTCAGAGCATCTGC AGTGTCC >IE_07B01_translated MNQNLHVLSMDSLPVGLRFRPTDEELIRYYLRRKINGHDDDVKAIREIDICKWEPWDLPDFSVIKTKDSEWLYFCPLDRKYPSGSRQNRATVAGYWKATG KDRKIKSGKTNIIGVKRTLVFHAGRAPRGTRTNWIIHEYRATEDDLSGTNPGQSPFVICKLFKKEELVLGEEDSKSDEVEEPAVSSPTVEVTKSEVSEVI KTEDVKRHDIAESSLVISGDSHSDACDEATTAELVDFKWYPELESLDFTLFSPLHSQVQSELGSSYNTFQPGSSNFSGNNNNSFQIQTQYGTNEVDTYIS DFLDSILKSPDEDPEKHKYVLQSGFDVVAPDQIAQVCQQGSAVDMSNDVSVTGIQIKSRQAQPSGYTNDYIAQGNGPRRLRLQSNFNGINTKNPELQAIK REAEDTVGESIKKRCGKLMRSKNVTGFVFKKITSVKCSYGGLFRAAVVAVVFLMSVCSLTVDFRASAVS |
|||
| Alignment |
◆CDS（TAIRver.9）_without_stop_codonとのalignment◆ 1406/1407 (99.9%)（アミノ酸配列で100%）一致しました。
10 20 30 40 50 60 70 80 90 100
AT3G49 ATGAATCAGAATCTTCATGTATTATCAATGGATTCGTTACCAGTTGGATTAAGATTCCGTCCAACAGACGAGGAGCTAATCCGTTACTATCTCCGTAGGA
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_07B ATGAATCAGAATCTTCATGTATTATCAATGGATTCGTTACCAGTTGGATTAAGATTCCGTCCAACAGACGAGGAGCTAATCCGTTACTATCTCCGTAGGA
10 20 30 40 50 60 70 80 90 100
110 120 130 140 150 160 170 180 190 200
AT3G49 AAATCAACGGTCACGATGACGACGTCAAAGCTATCCGTGAGATCGATATTTGCAAATGGGAACCTTGGGATTTACCTGATTTTTCTGTGATCAAAACTAA
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_07B AAATCAACGGTCACGATGACGACGTCAAAGCTATCCGTGAGATCGATATTTGCAAATGGGAACCTTGGGATTTACCTGATTTTTCTGTGATCAAAACTAA
110 120 130 140 150 160 170 180 190 200
210 220 230 240 250 260 270 280 290 300
AT3G49 AGACTCAGAGTGGCTCTACTTCTGTCCATTGGATCGGAAGTATCCGAGTGGAAGTAGACAGAACCGGGCAACAGTTGCAGGGTACTGGAAAGCCACAGGA
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_07B AGACTCAGAGTGGCTCTACTTCTGTCCATTGGATCGGAAGTATCCGAGTGGAAGTAGACAGAACCGGGCAACAGTTGCAGGGTACTGGAAAGCCACAGGA
210 220 230 240 250 260 270 280 290 300
310 320 330 340 350 360 370 380 390 400
AT3G49 AAAGACCGGAAGATAAAATCCGGTAAGACTAACATTATTGGTGTGAAGAGAACTCTAGTTTTCCACGCGGGTAGAGCTCCTAGGGGGACACGAACCAATT
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_07B AAAGACCGGAAGATAAAATCCGGTAAGACTAACATTATTGGTGTGAAGAGAACTCTAGTTTTCCACGCGGGTAGAGCTCCTAGGGGGACACGAACCAATT
310 320 330 340 350 360 370 380 390 400
410 420 430 440 450 460 470 480 490 500
AT3G49 GGATTATTCATGAGTATCGTGCCACGGAGGATGATCTTAGTGGTACCAATCCTGGCCAGAGTCCGTTTGTTATATGCAAATTGTTCAAGAAAGAAGAACT
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_07B GGATTATTCATGAGTATCGTGCCACGGAGGATGATCTTAGTGGTACCAATCCTGGCCAGAGTCCGTTTGTTATATGCAAATTGTTCAAGAAAGAAGAACT
410 420 430 440 450 460 470 480 490 500
510 520 530 540 550 560 570 580 590 600
AT3G49 GGTTTTAGGGGAAGAAGATTCGAAGTCAGATGAAGTTGAAGAACCTGCTGTCTCGTCTCCAACTGTCGAAGTGACTAAGTCAGAAGTATCTGAGGTAATT
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_07B GGTTTTAGGGGAAGAAGATTCGAAGTCAGATGAAGTTGAAGAACCTGCTGTCTCGTCTCCAACTGTCGAAGTGACTAAGTCAGAAGTATCTGAGGTAATT
510 520 530 540 550 560 570 580 590 600
610 620 630 640 650 660 670 680 690 700
AT3G49 AAAACAGAAGACGTGAAGCGTCATGACATAGCAGAATCTTCTCTTGTAATCTCTGGAGATTCTCATAGTGATGCTTGTGATGAGGCTACAACCGCAGAGC
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_07B AAAACAGAAGACGTGAAGCGTCATGACATAGCAGAATCTTCTCTTGTAATCTCTGGAGATTCTCATAGTGATGCTTGTGATGAGGCTACAACCGCAGAGC
610 620 630 640 650 660 670 680 690 700
710 720 730 740 750 760 770 780 790 800
AT3G49 TTGTAGATTTTAAATGGTATCCGGAATTGGAGTCCTTAGATTTCACGCTGTTCTCTCCATTACACTCTCAAGTCCAATCTGAGCTTGGATCCTCTTACAA
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_07B TTGTAGATTTTAAATGGTATCCGGAATTGGAGTCCTTAGATTTCACGCTGTTCTCTCCATTACACTCTCAAGTCCAATCTGAGCTTGGATCCTCTTACAA
710 720 730 740 750 760 770 780 790 800
810 820 830 840 850 860 870 880 890 900
AT3G49 CACATTCCAGCCTGGCTCGAGTAATTTTTCAGGGAACAACAACAACAGCTTCCAAATCCAGACTCAGTATGGTACAAATGAAGTAGATACGTATATATCT
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: ::::::::::::::::::::::::::::::
IE_07B CACATTCCAGCCTGGCTCGAGTAATTTTTCAGGGAACAACAACAACAGCTTCCAAATCCAGACTCAGTACGGTACAAATGAAGTAGATACGTATATATCT
810 820 830 840 850 860 870 880 890 900
910 920 930 940 950 960 970 980 990 1000
AT3G49 GATTTTCTTGATTCGATTCTCAAGAGCCCAGACGAGGATCCAGAGAAGCACAAGTATGTTTTGCAAAGTGGTTTTGATGTTGTAGCACCAGATCAGATTG
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_07B GATTTTCTTGATTCGATTCTCAAGAGCCCAGACGAGGATCCAGAGAAGCACAAGTATGTTTTGCAAAGTGGTTTTGATGTTGTAGCACCAGATCAGATTG
910 920 930 940 950 960 970 980 990 1000
1010 1020 1030 1040 1050 1060 1070 1080 1090 1100
AT3G49 CGCAAGTGTGTCAACAGGGCTCAGCAGTTGACATGAGCAATGATGTTTCAGTGACGGGTATCCAGATTAAGAGTCGCCAGGCACAACCCTCAGGTTATAC
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_07B CGCAAGTGTGTCAACAGGGCTCAGCAGTTGACATGAGCAATGATGTTTCAGTGACGGGTATCCAGATTAAGAGTCGCCAGGCACAACCCTCAGGTTATAC
1010 1020 1030 1040 1050 1060 1070 1080 1090 1100
1110 1120 1130 1140 1150 1160 1170 1180 1190 1200
AT3G49 CAATGACTATATAGCGCAGGGAAATGGTCCAAGAAGGCTGCGTCTGCAAAGTAACTTTAATGGCATTAACACGAAGAATCCTGAGCTTCAAGCTATAAAG
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_07B CAATGACTATATAGCGCAGGGAAATGGTCCAAGAAGGCTGCGTCTGCAAAGTAACTTTAATGGCATTAACACGAAGAATCCTGAGCTTCAAGCTATAAAG
1110 1120 1130 1140 1150 1160 1170 1180 1190 1200
1210 1220 1230 1240 1250 1260 1270 1280 1290 1300
AT3G49 AGAGAGGCTGAGGACACGGTTGGAGAAAGTATAAAGAAAAGGTGTGGAAAGCTAATGAGATCAAAGAACGTGACCGGTTTTGTGTTTAAAAAGATTACGT
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_07B AGAGAGGCTGAGGACACGGTTGGAGAAAGTATAAAGAAAAGGTGTGGAAAGCTAATGAGATCAAAGAACGTGACCGGTTTTGTGTTTAAAAAGATTACGT
1210 1220 1230 1240 1250 1260 1270 1280 1290 1300
1310 1320 1330 1340 1350 1360 1370 1380 1390 1400
AT3G49 CGGTGAAATGTTCATATGGAGGACTCTTTAGGGCGGCAGTAGTTGCAGTTGTGTTCTTGATGTCAGTCTGCAGTCTAACCGTCGACTTCAGAGCATCTGC
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_07B CGGTGAAATGTTCATATGGAGGACTCTTTAGGGCGGCAGTAGTTGCAGTTGTGTTCTTGATGTCAGTCTGCAGTCTAACCGTCGACTTCAGAGCATCTGC
1310 1320 1330 1340 1350 1360 1370 1380 1390 1400
AT3G49 AGTGTCC
:::::::
IE_07B AGTGTCC
10 20 30 40 50 60 70 80 90 100
AT3G49 MNQNLHVLSMDSLPVGLRFRPTDEELIRYYLRRKINGHDDDVKAIREIDICKWEPWDLPDFSVIKTKDSEWLYFCPLDRKYPSGSRQNRATVAGYWKATG
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_07B MNQNLHVLSMDSLPVGLRFRPTDEELIRYYLRRKINGHDDDVKAIREIDICKWEPWDLPDFSVIKTKDSEWLYFCPLDRKYPSGSRQNRATVAGYWKATG
10 20 30 40 50 60 70 80 90 100
110 120 130 140 150 160 170 180 190 200
AT3G49 KDRKIKSGKTNIIGVKRTLVFHAGRAPRGTRTNWIIHEYRATEDDLSGTNPGQSPFVICKLFKKEELVLGEEDSKSDEVEEPAVSSPTVEVTKSEVSEVI
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_07B KDRKIKSGKTNIIGVKRTLVFHAGRAPRGTRTNWIIHEYRATEDDLSGTNPGQSPFVICKLFKKEELVLGEEDSKSDEVEEPAVSSPTVEVTKSEVSEVI
110 120 130 140 150 160 170 180 190 200
210 220 230 240 250 260 270 280 290 300
AT3G49 KTEDVKRHDIAESSLVISGDSHSDACDEATTAELVDFKWYPELESLDFTLFSPLHSQVQSELGSSYNTFQPGSSNFSGNNNNSFQIQTQYGTNEVDTYIS
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_07B KTEDVKRHDIAESSLVISGDSHSDACDEATTAELVDFKWYPELESLDFTLFSPLHSQVQSELGSSYNTFQPGSSNFSGNNNNSFQIQTQYGTNEVDTYIS
210 220 230 240 250 260 270 280 290 300
310 320 330 340 350 360 370 380 390 400
AT3G49 DFLDSILKSPDEDPEKHKYVLQSGFDVVAPDQIAQVCQQGSAVDMSNDVSVTGIQIKSRQAQPSGYTNDYIAQGNGPRRLRLQSNFNGINTKNPELQAIK
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_07B DFLDSILKSPDEDPEKHKYVLQSGFDVVAPDQIAQVCQQGSAVDMSNDVSVTGIQIKSRQAQPSGYTNDYIAQGNGPRRLRLQSNFNGINTKNPELQAIK
310 320 330 340 350 360 370 380 390 400
410 420 430 440 450 460
AT3G49 REAEDTVGESIKKRCGKLMRSKNVTGFVFKKITSVKCSYGGLFRAAVVAVVFLMSVCSLTVDFRASAVS
:::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_07B REAEDTVGESIKKRCGKLMRSKNVTGFVFKKITSVKCSYGGLFRAAVVAVVFLMSVCSLTVDFRASAVS
410 420 430 440 450 460
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