| Code name | IE_07B03 | |||
|---|---|---|---|---|
| Locus | AT1G34390 | |||
| Template | IT_07B03 (ABRC_053) | |||
| Donor Vector | pDONR207 | |||
| Forward primer | IPF_07B03 (ggggacaagtttgtacaaaaaagcaggcttcATGGAAAGTGGCAACATTGTGAATGCA) | |||
| Reverse primer | IPR_07B03 (ggggaccactttgtacaagaaagctgggttCTGGACTTCAAGTTTTTGACCTCCTCTTTT) | |||
| Alignment with template seq. | 1st | 配列解析が行えなかったサンプルです。 | 2nd | アミノ酸配列100%一致 |
| Alignment with TAIR7CDS | - | アミノ酸配列100%一致 | ||
| comment | 1st:(Sequence解析まで行ったが、読み直す必要がある) | |||
| sequence |
>IE_07B03_1794bp ATGGAAAGTGGCAACATTGTGAATGCACAACCTGAATTATCAGGCATAATTGATGGATCCAAGAGTTATATGTACGAGCAGTTATGGAAACTCTGTGCGG GGCCTTTGTGTGATATTCCAAAACTTGGAGAAAAGATTTATTACTTTCCTCAAGGCAATATAGAGCTTGTTGAGGCATCTACAAGAGAAGAGTTGAATGA GTTAAAACCAATTTGTGATCTTCCTTCTAAGCTTCAATGTCGTGTTATTGCTATTCAACTTAAGGTGGAAAACAATAGTGATGAGACTTATGCTGAGATC ACTTTGATGCCAGATACAACTCAAGTTGTAATCCCTACTCAAAATGAAAATCAATTTAGACCATTAGTTAATTCCTTTACGAAGGTTTTAACGGCCTCGG ATACAAGCGGTGGATTCTTTGTTCCAAAAAAACATGCCATTGAATGTCTTCCTCCCCTGGATATGTCTCAGCCTCTACCGACACAAGAACTTCTTGCTAC AGATCTCCATGGTAATCAATGGAGATTCAACCACAACTATAGAGGTACACCGCAAAGACATTTGTTAACAACTGGTTGGAATGCATTCACAACATCAAAA AAATTGGTTGCAGGGGATGTTATTGTATTCGTTAGGGGAGAGACTGGAGAGTTACGAGTTGGTATCAGACGAGCAGGACATCAACAAGGGAATATACCTT CATCAATAATATCAATAGAAAGTATGAGACATGGGGTGATTGCTTCCGCAAAGCATGCTTTTGATAACCAATGTATGTTCATTGTGGTTTACAAGCCAAG ATCAAGTCAATTTATTGTCAGTTACGACAAATTCTTAGATGCTGTGAACAATAAGTTCAATGTTGGTTCAAGATTTACAATGCGGTTTGAGGGTGATGAT TTCTCTGAAAGAAGATATTTTGGGACAATTATTGGAGTTAGTGATTTCTCTCCTCATTGGAAGTGTTCAGAGTGGCGCAACTTAGAAGTGCAGTGGGATG AATTTGCATCATTTTCGAGACCTAATAAAGTGTCACCCTGGGAGATCGAACATTTAATGCCTGCGTTAAATGTTCCGCGACCATCTTTGCTCAAGAACAA GCGTTTACGAGAAGTTAATGAAATCGGTTCTTCATCATCACATCTCTTGCCTCCTATATTGACACAAGGACAAGAAATTGGCCAACTAAGCGTGGCCTCC CCGATGAATATTTCTCTCACTTATCGTGATACAACTGAAGATGTTATGAATCCTTCTAGATTGCTAATGAGCTACCCTGTCCAACCAATGCCCAAACTAA ATTACAATAACCAAATGGTTACACAAATAGAAGAAAATATAACAACCAAGACTGGCACTAATTTTAGGCTGTTTGGAGTCTCACTGGTCACTCCTTCAGT GATCAAAGATCCCATTGAAGAAATTGGCTCGGAGATTTCGAAACTTACTGAAGGAAAAAAGTTTGGTCAAAGCCAAACTTTGAGATCACCAACAGAGATC CAAAGCAAGCAGTTCAGTTCTACTAGAACTTGTACCAAAGTTCAAATGCAAGGTGTAACAATAGAAAGAGCTGTGGATTTAAGTGTTCTGAATGGATATG ATCAGCTAATACTAGAATTGGAGGAGCTCTTCGATCTCAAAGGCCAATTACAAACTCGCAACCAATGGGAAATAGCTTTCACAGATAGTGACGATGATAA GATGCTTGTTGGAGACGATCCATGGCCTGAATTCTGCAACATGGTGAAGAAGATATTGATATTCAAAAGAGGAGGTCAAAAACTTGAAGTCCAG >IE_07B03_translated MESGNIVNAQPELSGIIDGSKSYMYEQLWKLCAGPLCDIPKLGEKIYYFPQGNIELVEASTREELNELKPICDLPSKLQCRVIAIQLKVENNSDETYAEI TLMPDTTQVVIPTQNENQFRPLVNSFTKVLTASDTSGGFFVPKKHAIECLPPLDMSQPLPTQELLATDLHGNQWRFNHNYRGTPQRHLLTTGWNAFTTSK KLVAGDVIVFVRGETGELRVGIRRAGHQQGNIPSSIISIESMRHGVIASAKHAFDNQCMFIVVYKPRSSQFIVSYDKFLDAVNNKFNVGSRFTMRFEGDD FSERRYFGTIIGVSDFSPHWKCSEWRNLEVQWDEFASFSRPNKVSPWEIEHLMPALNVPRPSLLKNKRLREVNEIGSSSSHLLPPILTQGQEIGQLSVAS PMNISLTYRDTTEDVMNPSRLLMSYPVQPMPKLNYNNQMVTQIEENITTKTGTNFRLFGVSLVTPSVIKDPIEEIGSEISKLTEGKKFGQSQTLRSPTEI QSKQFSSTRTCTKVQMQGVTIERAVDLSVLNGYDQLILELEELFDLKGQLQTRNQWEIAFTDSDDDKMLVGDDPWPEFCNMVKKILIFKRGGQKLEVQ |
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| Alignment |
◆CDS（TAIRver.9）_without_stop_codonとのalignment◆ 100%一致しました。 |
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