âââIE_07B04 detailâââ

Code name	IE_07B04
Locus	AT1G35240
Template	IT_07B04 (ABRC_054)
Donor Vector	pDONR207
Forward primer	IPF_07B04 (ggggacaagtttgtacaaaaaagcaggcttcATGGAAACTGGCAACGTTGTGAATGCA)
Reverse primer	IPR_07B04 (ggggaccactttgtacaagaaagctgggttACTTGAGAGACTCTTACTGGACTTTAGGTT)
Alignment with template seq.	1st	éåè§£æãè¡ããªãã£ããµã³ãã«ã§ãã	2nd	ã¢ããé¸éå100%ä¸è´
Alignment with TAIR7CDS	1st	-	2nd	ã¢ããé¸éå100%ä¸è´
comment	1st:(Sequenceè§£æã¾ã§è¡ã£ããããã¬ã¼ã ã¯ãããªããå¡©åºç½®æããã), 2nd:70%, 36A→G, attL2上で1nt_sub
sequence	>IE_07B04_1770bp ATGGAAACTGGCAACGTTGTGAATGCACAACCTGAGTTATCAGGCATAATTGATGGATCCAAGAGCTATATGTACGAGCAGTTATGGAAACTCTGTGCGG GACCTTTGTGTGATATTCCAAAACTTGGAGAAAATGTTTATTACTTTCCTCAAGGCAATATAGAGCTTGTTGATGCATCTACAAGAGAAGAGTTGAATGA GTTACAACCAATTTGTGATCTTCCTTCTAAGCTTCAATGCCGTGTTATTGCAATTCATCTTAAGGTGGAAAACAATAGTGATGAGACTTATGCTGAGATC ACTTTGATGCCAGATACAACTCAAGTTGTAATCCCTACTCAGAGTGAAAATCAATTTAGGCCATTAGTTAATTCCTTTACGAAGGTTTTAACGGCCTCTG ATACAAGCGCTTATGGTGGATTCTTTGTTCCGAAAAAACATGCCATTGAATGTCTCCCTCCACTGCCTCTACCGGCACAAGAACTTCTTGCTAAAGATCT CCATGGTAATCAATGGAGATTCAGACACAGCTATAGAGGTACACCGCAAAGACATTCGTTAACAACTGGTTGGAATGAATTCACAACATCGAAAAAGTTG GTTAAAGGAGATGTTATTGTATTCGTTAGGGGAGAGACTGGAGAGTTACGAGTTGGTATCAGACGAGCAAGACATCAACAAGGGAATATACCTTCATCAA TAGTATCAATAGATTGTATGAGACATGGGGTAATTGCTTCCGCAAAGCATGCTCTTGATAACCAATGTATATTCATTGTGGTTTACAAGCCAAGTATCAG GTCAAGTCAATTTATTGTCAGTTACGACAAATTCTTAGACGCTATGAACAATAAGTTCATTGTCGGTTCAAGATTTACAATGCGGTTTGAGGGTGATGAT TTCTCTGAAAGAAGATATTTTGGGACAATTATTGGAGTTAATGATTTCTCTCCTCATTGGAAGTGTTCAGAGTGGCGCAGCTTAGAAGTTCAGTGGGATG AATTTGCATCATTTTCGAGACCTAATAAAGTGTCACCCTGGGAGATCGAACATTTAATGTCTGCGTTAAATGTTCCGCGATCATCTTTGCTCAAGAACAA GCGTTTACGAGAAGTTAATGAATTCGGACAAGAAATTGGACAACTAAGCGTCGCCTCCCCGATGAATACTTCTCTTCGTTATCGTGATACAACTGAAGAT GCTATGAATCCTTCTAGATTGCTAATGAGCTACCCTGTCCAACCAATGCCCAAACTAAATTACAATAACCAAATGGTTACACAAATAGAAGAAAATATAA CAACCAAGGCAGTCACTAATTTTAGGCTGTTTGGAGTCTCTCTAGCCATTCCTCTAGTGATCAAAGATCCCATTGAAGAAATTGGCTCGGATATTTCGAA ACTTACTGAAGGAAAAAAGTTTGGTCAAAGCCAAACTTTGAGATCACCAATAGAGATCCAAAGCAAGCAGTTTGGTTCTACTAGAACTTGTACCAAAGTT CAAATGCAAGGTGTAACAATAGGAAGAGCTGTGGATTTAAGTGTTCTTAATGGATATGATCAGCTAATACTAGAATTGGAGAAGCTCTTTGATCTCAAAG GCCAACTACAAACTCGCAACCAATGGAAAATAGCTTTCACAGATAGTGACGGGTATGAGATGCTTGTTGGAGACGATCCATGGCCTGAATTCTGCAAAAT GGTGAAGAAGATATTGATATATTCAAAAGAGGAGGTCAAAAACCTAAAGTCCAGTAAGAGTCTCTCAAGT >IE_07B04_translated METGNVVNAQPELSGIIDGSKSYMYEQLWKLCAGPLCDIPKLGENVYYFPQGNIELVDASTREELNELQPICDLPSKLQCRVIAIHLKVENNSDETYAEI TLMPDTTQVVIPTQSENQFRPLVNSFTKVLTASDTSAYGGFFVPKKHAIECLPPLPLPAQELLAKDLHGNQWRFRHSYRGTPQRHSLTTGWNEFTTSKKL VKGDVIVFVRGETGELRVGIRRARHQQGNIPSSIVSIDCMRHGVIASAKHALDNQCIFIVVYKPSIRSSQFIVSYDKFLDAMNNKFIVGSRFTMRFEGDD FSERRYFGTIIGVNDFSPHWKCSEWRSLEVQWDEFASFSRPNKVSPWEIEHLMSALNVPRSSLLKNKRLREVNEFGQEIGQLSVASPMNTSLRYRDTTED AMNPSRLLMSYPVQPMPKLNYNNQMVTQIEENITTKAVTNFRLFGVSLAIPLVIKDPIEEIGSDISKLTEGKKFGQSQTLRSPIEIQSKQFGSTRTCTKV QMQGVTIGRAVDLSVLNGYDQLILELEKLFDLKGQLQTRNQWKIAFTDSDGYEMLVGDDPWPEFCKMVKKILIYSKEEVKNLKSSKSLSS
Alignment	◆CDS（TAIRver.9）_without_stop_codonとのalignment◆ 1769/1770 (99.9%)（アミノ酸配列で100%）一致しました。 10 20 30 40 50 60 70 80 90 100 AT1G35 ATGGAAACTGGCAACGTTGTGAATGCACAACCTGAATTATCAGGCATAATTGATGGATCCAAGAGCTATATGTACGAGCAGTTATGGAAACTCTGTGCGG ::::::::::::::::::::::::::::::::::: :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_07B ATGGAAACTGGCAACGTTGTGAATGCACAACCTGAGTTATCAGGCATAATTGATGGATCCAAGAGCTATATGTACGAGCAGTTATGGAAACTCTGTGCGG 10 20 30 40 50 60 70 80 90 100 110 120 130 140 150 160 170 180 190 200 AT1G35 GACCTTTGTGTGATATTCCAAAACTTGGAGAAAATGTTTATTACTTTCCTCAAGGCAATATAGAGCTTGTTGATGCATCTACAAGAGAAGAGTTGAATGA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_07B GACCTTTGTGTGATATTCCAAAACTTGGAGAAAATGTTTATTACTTTCCTCAAGGCAATATAGAGCTTGTTGATGCATCTACAAGAGAAGAGTTGAATGA 110 120 130 140 150 160 170 180 190 200 210 220 230 240 250 260 270 280 290 300 AT1G35 GTTACAACCAATTTGTGATCTTCCTTCTAAGCTTCAATGCCGTGTTATTGCAATTCATCTTAAGGTGGAAAACAATAGTGATGAGACTTATGCTGAGATC :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_07B GTTACAACCAATTTGTGATCTTCCTTCTAAGCTTCAATGCCGTGTTATTGCAATTCATCTTAAGGTGGAAAACAATAGTGATGAGACTTATGCTGAGATC 210 220 230 240 250 260 270 280 290 300 310 320 330 340 350 360 370 380 390 400 AT1G35 ACTTTGATGCCAGATACAACTCAAGTTGTAATCCCTACTCAGAGTGAAAATCAATTTAGGCCATTAGTTAATTCCTTTACGAAGGTTTTAACGGCCTCTG :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_07B ACTTTGATGCCAGATACAACTCAAGTTGTAATCCCTACTCAGAGTGAAAATCAATTTAGGCCATTAGTTAATTCCTTTACGAAGGTTTTAACGGCCTCTG 310 320 330 340 350 360 370 380 390 400 410 420 430 440 450 460 470 480 490 500 AT1G35 ATACAAGCGCTTATGGTGGATTCTTTGTTCCGAAAAAACATGCCATTGAATGTCTCCCTCCACTGCCTCTACCGGCACAAGAACTTCTTGCTAAAGATCT :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_07B ATACAAGCGCTTATGGTGGATTCTTTGTTCCGAAAAAACATGCCATTGAATGTCTCCCTCCACTGCCTCTACCGGCACAAGAACTTCTTGCTAAAGATCT 410 420 430 440 450 460 470 480 490 500 510 520 530 540 550 560 570 580 590 600 AT1G35 CCATGGTAATCAATGGAGATTCAGACACAGCTATAGAGGTACACCGCAAAGACATTCGTTAACAACTGGTTGGAATGAATTCACAACATCGAAAAAGTTG :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_07B CCATGGTAATCAATGGAGATTCAGACACAGCTATAGAGGTACACCGCAAAGACATTCGTTAACAACTGGTTGGAATGAATTCACAACATCGAAAAAGTTG 510 520 530 540 550 560 570 580 590 600 610 620 630 640 650 660 670 680 690 700 AT1G35 GTTAAAGGAGATGTTATTGTATTCGTTAGGGGAGAGACTGGAGAGTTACGAGTTGGTATCAGACGAGCAAGACATCAACAAGGGAATATACCTTCATCAA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_07B GTTAAAGGAGATGTTATTGTATTCGTTAGGGGAGAGACTGGAGAGTTACGAGTTGGTATCAGACGAGCAAGACATCAACAAGGGAATATACCTTCATCAA 610 620 630 640 650 660 670 680 690 700 710 720 730 740 750 760 770 780 790 800 AT1G35 TAGTATCAATAGATTGTATGAGACATGGGGTAATTGCTTCCGCAAAGCATGCTCTTGATAACCAATGTATATTCATTGTGGTTTACAAGCCAAGTATCAG :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_07B TAGTATCAATAGATTGTATGAGACATGGGGTAATTGCTTCCGCAAAGCATGCTCTTGATAACCAATGTATATTCATTGTGGTTTACAAGCCAAGTATCAG 710 720 730 740 750 760 770 780 790 800 810 820 830 840 850 860 870 880 890 900 AT1G35 GTCAAGTCAATTTATTGTCAGTTACGACAAATTCTTAGACGCTATGAACAATAAGTTCATTGTCGGTTCAAGATTTACAATGCGGTTTGAGGGTGATGAT :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_07B GTCAAGTCAATTTATTGTCAGTTACGACAAATTCTTAGACGCTATGAACAATAAGTTCATTGTCGGTTCAAGATTTACAATGCGGTTTGAGGGTGATGAT 810 820 830 840 850 860 870 880 890 900 910 920 930 940 950 960 970 980 990 1000 AT1G35 TTCTCTGAAAGAAGATATTTTGGGACAATTATTGGAGTTAATGATTTCTCTCCTCATTGGAAGTGTTCAGAGTGGCGCAGCTTAGAAGTTCAGTGGGATG :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_07B TTCTCTGAAAGAAGATATTTTGGGACAATTATTGGAGTTAATGATTTCTCTCCTCATTGGAAGTGTTCAGAGTGGCGCAGCTTAGAAGTTCAGTGGGATG 910 920 930 940 950 960 970 980 990 1000 1010 1020 1030 1040 1050 1060 1070 1080 1090 1100 AT1G35 AATTTGCATCATTTTCGAGACCTAATAAAGTGTCACCCTGGGAGATCGAACATTTAATGTCTGCGTTAAATGTTCCGCGATCATCTTTGCTCAAGAACAA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_07B AATTTGCATCATTTTCGAGACCTAATAAAGTGTCACCCTGGGAGATCGAACATTTAATGTCTGCGTTAAATGTTCCGCGATCATCTTTGCTCAAGAACAA 1010 1020 1030 1040 1050 1060 1070 1080 1090 1100 1110 1120 1130 1140 1150 1160 1170 1180 1190 1200 AT1G35 GCGTTTACGAGAAGTTAATGAATTCGGACAAGAAATTGGACAACTAAGCGTCGCCTCCCCGATGAATACTTCTCTTCGTTATCGTGATACAACTGAAGAT :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_07B GCGTTTACGAGAAGTTAATGAATTCGGACAAGAAATTGGACAACTAAGCGTCGCCTCCCCGATGAATACTTCTCTTCGTTATCGTGATACAACTGAAGAT 1110 1120 1130 1140 1150 1160 1170 1180 1190 1200 1210 1220 1230 1240 1250 1260 1270 1280 1290 1300 AT1G35 GCTATGAATCCTTCTAGATTGCTAATGAGCTACCCTGTCCAACCAATGCCCAAACTAAATTACAATAACCAAATGGTTACACAAATAGAAGAAAATATAA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_07B GCTATGAATCCTTCTAGATTGCTAATGAGCTACCCTGTCCAACCAATGCCCAAACTAAATTACAATAACCAAATGGTTACACAAATAGAAGAAAATATAA 1210 1220 1230 1240 1250 1260 1270 1280 1290 1300 1310 1320 1330 1340 1350 1360 1370 1380 1390 1400 AT1G35 CAACCAAGGCAGTCACTAATTTTAGGCTGTTTGGAGTCTCTCTAGCCATTCCTCTAGTGATCAAAGATCCCATTGAAGAAATTGGCTCGGATATTTCGAA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_07B CAACCAAGGCAGTCACTAATTTTAGGCTGTTTGGAGTCTCTCTAGCCATTCCTCTAGTGATCAAAGATCCCATTGAAGAAATTGGCTCGGATATTTCGAA 1310 1320 1330 1340 1350 1360 1370 1380 1390 1400 1410 1420 1430 1440 1450 1460 1470 1480 1490 1500 AT1G35 ACTTACTGAAGGAAAAAAGTTTGGTCAAAGCCAAACTTTGAGATCACCAATAGAGATCCAAAGCAAGCAGTTTGGTTCTACTAGAACTTGTACCAAAGTT :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_07B ACTTACTGAAGGAAAAAAGTTTGGTCAAAGCCAAACTTTGAGATCACCAATAGAGATCCAAAGCAAGCAGTTTGGTTCTACTAGAACTTGTACCAAAGTT 1410 1420 1430 1440 1450 1460 1470 1480 1490 1500 1510 1520 1530 1540 1550 1560 1570 1580 1590 1600 AT1G35 CAAATGCAAGGTGTAACAATAGGAAGAGCTGTGGATTTAAGTGTTCTTAATGGATATGATCAGCTAATACTAGAATTGGAGAAGCTCTTTGATCTCAAAG :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_07B CAAATGCAAGGTGTAACAATAGGAAGAGCTGTGGATTTAAGTGTTCTTAATGGATATGATCAGCTAATACTAGAATTGGAGAAGCTCTTTGATCTCAAAG 1510 1520 1530 1540 1550 1560 1570 1580 1590 1600 1610 1620 1630 1640 1650 1660 1670 1680 1690 1700 AT1G35 GCCAACTACAAACTCGCAACCAATGGAAAATAGCTTTCACAGATAGTGACGGGTATGAGATGCTTGTTGGAGACGATCCATGGCCTGAATTCTGCAAAAT :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_07B GCCAACTACAAACTCGCAACCAATGGAAAATAGCTTTCACAGATAGTGACGGGTATGAGATGCTTGTTGGAGACGATCCATGGCCTGAATTCTGCAAAAT 1610 1620 1630 1640 1650 1660 1670 1680 1690 1700 1710 1720 1730 1740 1750 1760 1770 AT1G35 GGTGAAGAAGATATTGATATATTCAAAAGAGGAGGTCAAAAACCTAAAGTCCAGTAAGAGTCTCTCAAGT :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_07B GGTGAAGAAGATATTGATATATTCAAAAGAGGAGGTCAAAAACCTAAAGTCCAGTAAGAGTCTCTCAAGT 1710 1720 1730 1740 1750 1760 1770 10 20 30 40 50 60 70 80 90 100 AT1G35 METGNVVNAQPELSGIIDGSKSYMYEQLWKLCAGPLCDIPKLGENVYYFPQGNIELVDASTREELNELQPICDLPSKLQCRVIAIHLKVENNSDETYAEI :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_07B METGNVVNAQPELSGIIDGSKSYMYEQLWKLCAGPLCDIPKLGENVYYFPQGNIELVDASTREELNELQPICDLPSKLQCRVIAIHLKVENNSDETYAEI 10 20 30 40 50 60 70 80 90 100 110 120 130 140 150 160 170 180 190 200 AT1G35 TLMPDTTQVVIPTQSENQFRPLVNSFTKVLTASDTSAYGGFFVPKKHAIECLPPLPLPAQELLAKDLHGNQWRFRHSYRGTPQRHSLTTGWNEFTTSKKL :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_07B TLMPDTTQVVIPTQSENQFRPLVNSFTKVLTASDTSAYGGFFVPKKHAIECLPPLPLPAQELLAKDLHGNQWRFRHSYRGTPQRHSLTTGWNEFTTSKKL 110 120 130 140 150 160 170 180 190 200 210 220 230 240 250 260 270 280 290 300 AT1G35 VKGDVIVFVRGETGELRVGIRRARHQQGNIPSSIVSIDCMRHGVIASAKHALDNQCIFIVVYKPSIRSSQFIVSYDKFLDAMNNKFIVGSRFTMRFEGDD :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_07B VKGDVIVFVRGETGELRVGIRRARHQQGNIPSSIVSIDCMRHGVIASAKHALDNQCIFIVVYKPSIRSSQFIVSYDKFLDAMNNKFIVGSRFTMRFEGDD 210 220 230 240 250 260 270 280 290 300 310 320 330 340 350 360 370 380 390 400 AT1G35 FSERRYFGTIIGVNDFSPHWKCSEWRSLEVQWDEFASFSRPNKVSPWEIEHLMSALNVPRSSLLKNKRLREVNEFGQEIGQLSVASPMNTSLRYRDTTED :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_07B FSERRYFGTIIGVNDFSPHWKCSEWRSLEVQWDEFASFSRPNKVSPWEIEHLMSALNVPRSSLLKNKRLREVNEFGQEIGQLSVASPMNTSLRYRDTTED 310 320 330 340 350 360 370 380 390 400 410 420 430 440 450 460 470 480 490 500 AT1G35 AMNPSRLLMSYPVQPMPKLNYNNQMVTQIEENITTKAVTNFRLFGVSLAIPLVIKDPIEEIGSDISKLTEGKKFGQSQTLRSPIEIQSKQFGSTRTCTKV :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_07B AMNPSRLLMSYPVQPMPKLNYNNQMVTQIEENITTKAVTNFRLFGVSLAIPLVIKDPIEEIGSDISKLTEGKKFGQSQTLRSPIEIQSKQFGSTRTCTKV 410 420 430 440 450 460 470 480 490 500 510 520 530 540 550 560 570 580 590 AT1G35 QMQGVTIGRAVDLSVLNGYDQLILELEKLFDLKGQLQTRNQWKIAFTDSDGYEMLVGDDPWPEFCKMVKKILIYSKEEVKNLKSSKSLSS :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_07B QMQGVTIGRAVDLSVLNGYDQLILELEKLFDLKGQLQTRNQWKIAFTDSDGYEMLVGDDPWPEFCKMVKKILIYSKEEVKNLKSSKSLSS 510 520 530 540 550 560 570 580 590

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âââIE_07B04 detailâââ