| Code name | IE_07B05 | |||
|---|---|---|---|---|
| Locus | AT1G77850 | |||
| Template | IT_07B05 (ABRC_055) | |||
| Donor Vector | pDONR207 | |||
| Forward primer | IPF_07B05 (ggggacaagtttgtacaaaaaagcaggcttcATGTCACCGCCGTCGGCAACCGCCGGT) | |||
| Reverse primer | IPR_07B05 (ggggaccactttgtacaagaaagctgggttACCTTGGGAGCTAGAACCTGCGTTGCTGTT) | |||
| Alignment with template seq. | 1st | 配列解析が行えなかったサンプルです。 | 2nd | アミノ酸配列100%一致 |
| Alignment with TAIR7CDS | - | アミノ酸配列100%一致 | ||
| comment | 1st:(Sequence解析まで行ったが、読み直す必要がある) | |||
| sequence |
>IE_07B05_1755bp ATGTCACCGCCGTCGGCAACCGCCGGTGACATCAACCACCGTGAAGTAGACCCGACGATCTGGCGCGCTTGTGCTGGAGCCTCCGTCCAGATCCCTGTCC TTCACTCTAGGGTTTACTACTTTCCACAAGGTCACGTTGAGCACTGTTGCCCTCTCCTCTCTACTCTTCCTTCCTCCACCTCGCCGGTTCCATGTATCAT CACTTCAATTCAGTTGCTCGCCGATCCGGTCACCGACGAGGTCTTTGCTCACCTTATTCTTCAACCGATGACGCAGCAGCAGTTTACTCCGACTAATTAT TCACGATTCGGCAGATTCGATGGCGATGTTGATGATAACAACAAGGTGACTACCTTCGCCAAAATTCTCACGCCTTCTGATGCTAACAATGGAGGTGGCT TCTCCGTTCCTCGTTTCTGTGCTGATTCCGTCTTCCCTCTGCTTAATTTTCAAATCGATCCACCGGTTCAGAAGCTCTACGTCACTGATATCCATGGAGC TGTTTGGGATTTCAGGCATATCTATCGCGGTACACCGAGGCGTCACTTGCTAACAACGGGATGGAGTAAGTTCGTCAATAGCAAGAAGCTCATCGCTGGA GATTCGGTGGTGTTTATGAGAAAATCTGCAGATGAGATGTTCATCGGCGTTAGGAGAACTCCGATCTCAAGCAGCGACGGAGGAAGTAGCTATTACGGAG GAGATGAGTATAACGGTTACTACAGTCAGAGTAGCGTTGCCAAGGAAGATGATGGGAGTCCGAAGAAGACGTTTAGGAGATCTGGGAATGGTAAGTTGAC TGCTGAGGCTGTGACGGATGCGATCAATAGAGCGTCTCAGGGATTACCATTTGAGGTGGTGTTTTATCCGGCTGCTGGATGGTCTGAGTTTGTTGTGAGA GCTGAAGATGTTGAGTCTTCAATGTCTATGTATTGGACTCCTGGGACTCGAGTCAAGATGGCTATGGAGACTGAAGATTCTTCTCGGATCACATGGTTTC AAGGCATCGTTTCCTCTACTTATCAGGAGACCGGTCCATGGCGTGGATCTCCATGGAAGCAGCTTCAGATCACATGGGATGAACCTGAGATTCTGCAAAA CGTGAAGAGGGTGAATCCATGGCAAGTGGAAATTGCTGCACATGCAACTCAACTGCATACCCCTTTCCCTCCAGCAAAGAGGTTGAAGTATCCACAACCC GGAGGAGGGTTCTTGAGTGGAGATGATGGAGAAATCCTTTATCCTCAAAGTGGACTGTCTAGTGCAGCAGCACCTGATCCAAGTCCTTCTATGTTCTCGT ATTCTACATTTCCTGCTGGCATGCAGGGAGCCAGGCAATATGATTTTGGGTCTTTCAATCCAACCGGATTCATTGGAGGAAATCCTCCCCAGCTATTCAC CAATAACTTCTTAAGTCCGCTTCCTGATTTGGGAAAAGTGTCGACTGAGATGATGAACTTTGGCAGTCCGCCATCAGATAACTTATCGCCTAATAGCAAC ACCACTAATCTGTCCTCTGGAAATGACCTGGTTGGAAACCGAGGCCCCCTTTCAAAGAAAGTTAACTCGATTCAGTTGTTTGGCAAGATCATTACCGTGG AGGAGCATTCTGAGAGCGGTCCTGCAGAGTCTGGCTTGTGTGAAGAGGATGGCAGCAAAGAGTCCAGCGACAATGAGACACAGTTGTCCTTATCACATGC TCCTCCAAGCGTGCCTAAACATTCCAACAGCAACGCAGGTTCTAGCTCCCAAGGT >IE_07B05_translated MSPPSATAGDINHREVDPTIWRACAGASVQIPVLHSRVYYFPQGHVEHCCPLLSTLPSSTSPVPCIITSIQLLADPVTDEVFAHLILQPMTQQQFTPTNY SRFGRFDGDVDDNNKVTTFAKILTPSDANNGGGFSVPRFCADSVFPLLNFQIDPPVQKLYVTDIHGAVWDFRHIYRGTPRRHLLTTGWSKFVNSKKLIAG DSVVFMRKSADEMFIGVRRTPISSSDGGSSYYGGDEYNGYYSQSSVAKEDDGSPKKTFRRSGNGKLTAEAVTDAINRASQGLPFEVVFYPAAGWSEFVVR AEDVESSMSMYWTPGTRVKMAMETEDSSRITWFQGIVSSTYQETGPWRGSPWKQLQITWDEPEILQNVKRVNPWQVEIAAHATQLHTPFPPAKRLKYPQP GGGFLSGDDGEILYPQSGLSSAAAPDPSPSMFSYSTFPAGMQGARQYDFGSFNPTGFIGGNPPQLFTNNFLSPLPDLGKVSTEMMNFGSPPSDNLSPNSN TTNLSSGNDLVGNRGPLSKKVNSIQLFGKIITVEEHSESGPAESGLCEEDGSKESSDNETQLSLSHAPPSVPKHSNSNAGSSSQG |
|||
| Alignment |
◆CDS（TAIRver.9）_without_stop_codonとのalignment◆ 100%一致しました。 |
|||