| Code name | IE_07B09 | |||
|---|---|---|---|---|
| Locus | AT2G31220 | |||
| Template | IT_07B09 (ABRC_075) | |||
| Donor Vector | pDONR207 | |||
| Forward primer | IPF_07B09 (ggggacaagtttgtacaaaaaagcaggcttcATGGAAGAAGAAAGAGAAAGCCTTTAC) | |||
| Reverse primer | IPR_07B09 (ggggaccactttgtacaagaaagctgggttGTAGCCGTTGCTAGGAACAGCTTCCATGTA) | |||
| Alignment with template seq. | 1st | 配列解析が行えなかったサンプルです。 | 2nd | アミノ酸配列100%一致 |
| Alignment with TAIR7CDS | - | アミノ酸配列100%一致 | ||
| comment | 1st:(Sequence解析まで行ったが、読み直す必要がある) | |||
| sequence |
>IE_07B09_1374bp ATGGAAGAAGAAAGAGAAAGCCTTTACGAGGAAATGGGTTGTTTCGATCCCAACACTCCAGCAGAAGTTACGGTGGAGAGTAGCTTCTCTCAAGCCGAAC CACCACCACCACCACCGCAAGTTCTGGTTGCCGGAAGCACAAGCAACAGCAACTGCAGTGTTGAGGTAGAAGAGCTCTCTGAATTCCATCTCAGCCCACA AGATTGTCCTCAAGCTTCTTCAACACCTCTCCAGTTTCACATCAATCCTCCTCCTCCACCACCTCCTCCTTGTGATCAACTCCACAACAATTTGATTCAC CAAATGGCTTCACATCAACAACAACACTCTAATTGGGATAATGGGTATCAGGATTTTGTGAATTTGGGACCTAACTCTGCAACAACTCCTGATTTGCTTA GTCTCTTACACTTGCCTAGATGCTCATTGCCTCCTAATCATCATCCTTCCTCTATGCTACCTACCTCTTTCTCAGACATTATGTCTTCTTCTTCTGCTGC TGCTGTTATGTACGACCCTCTCTTCCATTTGAATTTCCCTATGCAGCCTAGAGATCAGAACCAGCTAAGAAACGGCTCTTGTTTACTTGGAGTTGAAGAT CAAATTCAGATGGATGCTAATGGAGGAATGAATGTTTTGTACTTTGAAGGAGCAAACAACAACAATGGTGGATTTGAGAATGAGATTCTTGAGTTTAACA ATGGAGTTACTCGTAAAGGAAGAGGATCAAGAAAGTCGAGAACTTCCCCTACAGAACGTGAGAGACGAGTTCACTTCAATGATCGTTTCTTCGACTTAAA GAACCTCATTCCAAATCCCACAAAGATTGATAGAGCATCGATAGTTGGAGAGGCGATAGATTACATCAAAGAGCTTTTAAGGACAATAGAGGAGTTTAAG ATGCTTGTGGAGAAGAAGAGATGTGGGAGATTCAGGAGCAAGAAGAGAGCTAGAGTTGGTGAAGGAGGAGGAGGAGAAGATCAAGAAGAAGAAGAAGACA CTGTGAATTACAAGCCACAGAGCGAAGTAGACCAGTCTTGCTTCAACAAGAACAACAACAACTCACTGAGATGTTCATGGCTTAAGAGGAAATCGAAAGT CACTGAGGTCGATGTACGTATAATCGATGATGAAGTAACCATCAAGCTTGTTCAGAAGAAGAAGATTAACTGTTTGTTGTTCACCACCAAAGTTCTTGAT CAGCTTCAGCTAGATCTTCACCATGTTGCAGGTGGACAGATTGGTGAACATTACAGCTTCTTGTTCAACACTAAGATTTGTGAAGGATCTTGTGTGTATG CAAGTGGAATTGCAGACACATTGATGGAGGTCGTGGAGAAGCAGTACATGGAAGCTGTTCCTAGCAACGGCTAC >IE_07B09_translated MEEERESLYEEMGCFDPNTPAEVTVESSFSQAEPPPPPPQVLVAGSTSNSNCSVEVEELSEFHLSPQDCPQASSTPLQFHINPPPPPPPPCDQLHNNLIH QMASHQQQHSNWDNGYQDFVNLGPNSATTPDLLSLLHLPRCSLPPNHHPSSMLPTSFSDIMSSSSAAAVMYDPLFHLNFPMQPRDQNQLRNGSCLLGVED QIQMDANGGMNVLYFEGANNNNGGFENEILEFNNGVTRKGRGSRKSRTSPTERERRVHFNDRFFDLKNLIPNPTKIDRASIVGEAIDYIKELLRTIEEFK MLVEKKRCGRFRSKKRARVGEGGGGEDQEEEEDTVNYKPQSEVDQSCFNKNNNNSLRCSWLKRKSKVTEVDVRIIDDEVTIKLVQKKKINCLLFTTKVLD QLQLDLHHVAGGQIGEHYSFLFNTKICEGSCVYASGIADTLMEVVEKQYMEAVPSNGY |
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| Alignment |
◆CDS（TAIRver.9）_without_stop_codonとのalignment◆ 100%一致しました。 |
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