◆◆◆IE_07B11 detail◆◆◆

Code name	IE_07B11
Locus	AT5G60470
Template	IT_07B11 (ABRC_078)
Donor Vector	pDONR207
Forward primer	IPF_07B11 (ggggacaagtttgtacaaaaaagcaggcttcATGGCGACAAATCGGTTCTTTTGTGAA)
Reverse primer	IPR_07B11 (ggggaccactttgtacaagaaagctgggttTGGTTGCTTCCTGCCTACAATGGTTGCGGC)
Alignment with template seq.	1st	アミノ酸配列100%一致	2nd	-
Alignment with TAIR7CDS	1st	アミノ酸配列100%一致	2nd	-
comment
sequence	>IE_07B11_1176bp ATGGCGACAAATCGGTTCTTTTGTGAAATTTGTAACAAGGGGTTTCAGAGAGAACAGAACCTTCAGCTCCACAAAAGAGGCCACAACCTGCCATGGAAGC TGAAGCAGAAAACGAACAAGAATCAGGTGAAGAAGAAAGTTTATATATGCCCTGAGAAGAGTTGCGTCCATCATGATCCAGCTCGAGCCCTAGGAGACCT TACTGGAATAAAGAAGCACTTTAGCAGGAAACATGGAGAGAAGAAGTGGAAATGCGATAAGTGTTCCAAGAAGTATGCTGTGATATCCGATTGGAAAGCT CATAACAAGATTTGTGGTAGCAGAGAGTTTAGATGTGATTGTGGTACTCTTTTCTCCAGGAAGGACAGTTTCATCTCACATAGATCCTTCTGTGATGTCT TAGCTGAAGAAAGTTCTAAATTCTTCTCAGTTCCTTCACCATTAGCCGCCAATTCGACCATCGCCACTGTCACTGATACCAACAATCCAATTCTCATTCA ATCACAATTGGATCAATCTTCCACAGGTACGGCCGATCTTAACGTCAACAACAATCACACCACCCTATTTGGACAAAAGTTCACCAACTCCAACCCAACC CAACAACAACCCAATGCTTTGGCGTTGTCCTCACCACCATCACCTCGTAGTACATCTGATTCGGTTCATAATCTATGGAAGTTACAAGAAGAGGAATGTG CACACCAGTGGTTACTCAATGAATATATGAACAATAACAAAAACATATTCCATAAGGGAATATTCAAGAATCAAGAAGATGAGATAAAGAAGGGAAATAT CTACAGCGGCTCAAACCCAACCGATGGAAACATAGCATCCTTGTTCTCGTATAATCAAGAAGCAGTGAATATGGCTTCATTTTCGGCAACAACATTGCTT CAAAAGGTAGCTCAAACGGGAACACCATCAAGCTCCGAAACTAGCACCACCATGTTTGGGCAAATGACTTCCTCCATCTTCAACAATACGATGCTAAACT CCTATTGTTTAACGGCGAAGAATAATGAAGAAGAGTTGACCAGAGACTTTCTTGGTGTGGGAAGTAGTGAGGACCAACAACGTCTACATCGTCATCGCTT CCCATCAAGCGTGCCATTGACGGCGAACCATGATATTCCCAAGTTGGCCGCAACCATTGTAGGCAGGAAGCAACCA >IE_07B11_translated MATNRFFCEICNKGFQREQNLQLHKRGHNLPWKLKQKTNKNQVKKKVYICPEKSCVHHDPARALGDLTGIKKHFSRKHGEKKWKCDKCSKKYAVISDWKA HNKICGSREFRCDCGTLFSRKDSFISHRSFCDVLAEESSKFFSVPSPLAANSTIATVTDTNNPILIQSQLDQSSTGTADLNVNNNHTTLFGQKFTNSNPT QQQPNALALSSPPSPRSTSDSVHNLWKLQEEECAHQWLLNEYMNNNKNIFHKGIFKNQEDEIKKGNIYSGSNPTDGNIASLFSYNQEAVNMASFSATTLL QKVAQTGTPSSSETSTTMFGQMTSSIFNNTMLNSYCLTAKNNEEELTRDFLGVGSSEDQQRLHRHRFPSSVPLTANHDIPKLAATIVGRKQP
Alignment	◆CDS（TAIRver.9）_without_stop_codonとのalignment◆ 1176/1350 (87.1%)（アミノ酸配列で392/450 (87.1%)）一致しました。 10 20 30 40 50 60 70 80 90 100 AT5G60 ATGACCGCCTCTCATTCTCTAGCTTTGTCCATGACCAAGAGCACATCACCCCTAATCCTTACCCTAACTCTCAGCCTGCAGCCTCCACTAAAACACCCAA IE_07B ---------------------------------------------------------------------------------------------------- 110 120 130 140 150 160 170 180 190 200 AT5G60 GAAAAAGAGAAACCTTCCAGGAAATCCAGGCATATCCAAATGCAGAAGTCATCTCTCTATCTCCAAAATCCCTCATGGCGACAAATCGGTTCTTTTGTGA :::::::::::::::::::::::::: IE_07B --------------------------------------------------------------------------ATGGCGACAAATCGGTTCTTTTGTGA 10 20 210 220 230 240 250 260 270 280 290 300 AT5G60 AATTTGTAACAAGGGGTTTCAGAGAGAACAGAACCTTCAGCTCCACAAAAGAGGCCACAACCTGCCATGGAAGCTGAAGCAGAAAACGAACAAGAATCAG :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_07B AATTTGTAACAAGGGGTTTCAGAGAGAACAGAACCTTCAGCTCCACAAAAGAGGCCACAACCTGCCATGGAAGCTGAAGCAGAAAACGAACAAGAATCAG 30 40 50 60 70 80 90 100 110 120 310 320 330 340 350 360 370 380 390 400 AT5G60 GTGAAGAAGAAAGTTTATATATGCCCTGAGAAGAGTTGCGTCCATCATGATCCAGCTCGAGCCCTAGGAGACCTTACTGGAATAAAGAAGCACTTTAGCA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_07B GTGAAGAAGAAAGTTTATATATGCCCTGAGAAGAGTTGCGTCCATCATGATCCAGCTCGAGCCCTAGGAGACCTTACTGGAATAAAGAAGCACTTTAGCA 130 140 150 160 170 180 190 200 210 220 410 420 430 440 450 460 470 480 490 500 AT5G60 GGAAACATGGAGAGAAGAAGTGGAAATGCGATAAGTGTTCCAAGAAGTATGCTGTGATATCCGATTGGAAAGCTCATAACAAGATTTGTGGTAGCAGAGA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_07B GGAAACATGGAGAGAAGAAGTGGAAATGCGATAAGTGTTCCAAGAAGTATGCTGTGATATCCGATTGGAAAGCTCATAACAAGATTTGTGGTAGCAGAGA 230 240 250 260 270 280 290 300 310 320 510 520 530 540 550 560 570 580 590 600 AT5G60 GTTTAGATGTGATTGTGGTACTCTTTTCTCCAGGAAGGACAGTTTCATCTCACATAGATCCTTCTGTGATGTCTTAGCTGAAGAAAGTTCTAAATTCTTC :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_07B GTTTAGATGTGATTGTGGTACTCTTTTCTCCAGGAAGGACAGTTTCATCTCACATAGATCCTTCTGTGATGTCTTAGCTGAAGAAAGTTCTAAATTCTTC 330 340 350 360 370 380 390 400 410 420 610 620 630 640 650 660 670 680 690 700 AT5G60 TCAGTTCCTTCACCATTAGCCGCCAATTCGACCATCGCCACTGTCACTGATACCAACAATCCAATTCTCATTCAATCACAATTGGATCAATCTTCCACAG :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_07B TCAGTTCCTTCACCATTAGCCGCCAATTCGACCATCGCCACTGTCACTGATACCAACAATCCAATTCTCATTCAATCACAATTGGATCAATCTTCCACAG 430 440 450 460 470 480 490 500 510 520 710 720 730 740 750 760 770 780 790 800 AT5G60 GTACGGCCGATCTTAACGTCAACAACAATCACACCACCCTATTTGGACAAAAGTTCACCAACTCCAACCCAACCCAACAACAACCCAATGCTTTGGCGTT :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_07B GTACGGCCGATCTTAACGTCAACAACAATCACACCACCCTATTTGGACAAAAGTTCACCAACTCCAACCCAACCCAACAACAACCCAATGCTTTGGCGTT 530 540 550 560 570 580 590 600 610 620 810 820 830 840 850 860 870 880 890 900 AT5G60 GTCCTCACCACCATCACCTCGTAGTACATCTGATTCGGTTCATAATCTATGGAAGTTACAAGAAGAGGAATGTGCACACCAGTGGTTACTCAATGAATAT :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_07B GTCCTCACCACCATCACCTCGTAGTACATCTGATTCGGTTCATAATCTATGGAAGTTACAAGAAGAGGAATGTGCACACCAGTGGTTACTCAATGAATAT 630 640 650 660 670 680 690 700 710 720 910 920 930 940 950 960 970 980 990 1000 AT5G60 ATGAACAATAACAAAAACATATTCCATAAGGGAATATTCAAGAATCAAGAAGATGAGATAAAGAAGGGAAATATCTACAGCGGCTCAAACCCAACCGATG :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_07B ATGAACAATAACAAAAACATATTCCATAAGGGAATATTCAAGAATCAAGAAGATGAGATAAAGAAGGGAAATATCTACAGCGGCTCAAACCCAACCGATG 730 740 750 760 770 780 790 800 810 820 1010 1020 1030 1040 1050 1060 1070 1080 1090 1100 AT5G60 GAAACATAGCATCCTTGTTCTCGTATAATCAAGAAGCAGTGAATATGGCTTCATTTTCGGCAACAACATTGCTTCAAAAGGTAGCTCAAACGGGAACACC :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_07B GAAACATAGCATCCTTGTTCTCGTATAATCAAGAAGCAGTGAATATGGCTTCATTTTCGGCAACAACATTGCTTCAAAAGGTAGCTCAAACGGGAACACC 830 840 850 860 870 880 890 900 910 920 1110 1120 1130 1140 1150 1160 1170 1180 1190 1200 AT5G60 ATCAAGCTCCGAAACTAGCACCACCATGTTTGGGCAAATGACTTCCTCCATCTTCAACAATACGATGCTAAACTCCTATTGTTTAACGGCGAAGAATAAT :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_07B ATCAAGCTCCGAAACTAGCACCACCATGTTTGGGCAAATGACTTCCTCCATCTTCAACAATACGATGCTAAACTCCTATTGTTTAACGGCGAAGAATAAT 930 940 950 960 970 980 990 1000 1010 1020 1210 1220 1230 1240 1250 1260 1270 1280 1290 1300 AT5G60 GAAGAAGAGTTGACCAGAGACTTTCTTGGTGTGGGAAGTAGTGAGGACCAACAACGTCTACATCGTCATCGCTTCCCATCAAGCGTGCCATTGACGGCGA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_07B GAAGAAGAGTTGACCAGAGACTTTCTTGGTGTGGGAAGTAGTGAGGACCAACAACGTCTACATCGTCATCGCTTCCCATCAAGCGTGCCATTGACGGCGA 1030 1040 1050 1060 1070 1080 1090 1100 1110 1120 1310 1320 1330 1340 1350 AT5G60 ACCATGATATTCCCAAGTTGGCCGCAACCATTGTAGGCAGGAAGCAACCA :::::::::::::::::::::::::::::::::::::::::::::::::: IE_07B ACCATGATATTCCCAAGTTGGCCGCAACCATTGTAGGCAGGAAGCAACCA 1130 1140 1150 1160 1170 10 20 30 40 50 60 70 80 90 100 AT5G60 MTASHSLALSMTKSTSPLILTLTLSLQPPLKHPRKRETFQEIQAYPNAEVISLSPKSLMATNRFFCEICNKGFQREQNLQLHKRGHNLPWKLKQKTNKNQ :::::::::::::::::::::::::::::::::::::::::: IE_07B ----------------------------------------------------------MATNRFFCEICNKGFQREQNLQLHKRGHNLPWKLKQKTNKNQ 10 20 30 40 110 120 130 140 150 160 170 180 190 200 AT5G60 VKKKVYICPEKSCVHHDPARALGDLTGIKKHFSRKHGEKKWKCDKCSKKYAVISDWKAHNKICGSREFRCDCGTLFSRKDSFISHRSFCDVLAEESSKFF :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_07B VKKKVYICPEKSCVHHDPARALGDLTGIKKHFSRKHGEKKWKCDKCSKKYAVISDWKAHNKICGSREFRCDCGTLFSRKDSFISHRSFCDVLAEESSKFF 50 60 70 80 90 100 110 120 130 140 210 220 230 240 250 260 270 280 290 300 AT5G60 SVPSPLAANSTIATVTDTNNPILIQSQLDQSSTGTADLNVNNNHTTLFGQKFTNSNPTQQQPNALALSSPPSPRSTSDSVHNLWKLQEEECAHQWLLNEY :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_07B SVPSPLAANSTIATVTDTNNPILIQSQLDQSSTGTADLNVNNNHTTLFGQKFTNSNPTQQQPNALALSSPPSPRSTSDSVHNLWKLQEEECAHQWLLNEY 150 160 170 180 190 200 210 220 230 240 310 320 330 340 350 360 370 380 390 400 AT5G60 MNNNKNIFHKGIFKNQEDEIKKGNIYSGSNPTDGNIASLFSYNQEAVNMASFSATTLLQKVAQTGTPSSSETSTTMFGQMTSSIFNNTMLNSYCLTAKNN :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_07B MNNNKNIFHKGIFKNQEDEIKKGNIYSGSNPTDGNIASLFSYNQEAVNMASFSATTLLQKVAQTGTPSSSETSTTMFGQMTSSIFNNTMLNSYCLTAKNN 250 260 270 280 290 300 310 320 330 340 410 420 430 440 450 AT5G60 EEELTRDFLGVGSSEDQQRLHRHRFPSSVPLTANHDIPKLAATIVGRKQP :::::::::::::::::::::::::::::::::::::::::::::::::: IE_07B EEELTRDFLGVGSSEDQQRLHRHRFPSSVPLTANHDIPKLAATIVGRKQP 350 360 370 380 390

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