◆◆◆IE_07D07 detail◆◆◆

Code nameIE_07D07
LocusAT3G01030
TemplateIT_07D07 (ABRC_167)
Donor VectorpDONR207
Forward primerIPF_07D07 (ggggacaagtttgtacaaaaaagcaggcttcATGGAAGAACAACAAGAATTTGAGAAA)
Reverse primerIPR_07D07 (ggggaccactttgtacaagaaagctgggttAAGCATGTACTGACCTAAACAGCCTTCAGC)
Alignment with template seq.1stアミノ酸配列100%一致2nd-
Alignment with TAIR7CDSアミノ酸配列100%一致-
comment
sequence 
>IE_07D07_1113bp
ATGGAAGAACAACAAGAATTTGAGAAACCCATCTTCGAATTCCGTCCTAAGAAGCTCAGAACCTCTCGAGTTTCGAGAAATCTCATGAAGAAGACTGGCT
TCAGAGAATCTATGAATCATTACGAAGAGCTCTCTTGCAACTACGGATTGAGGGAAAACCCGAAGAAAACCCAGAAATCTTTACTCGATCATCGATTCTG
TACGAGGAGGAGGAGAAATAAAAAGATCCTGATTCGATGCAAAGAATGCGGGAAAGGGTTTCTTTACGAGAAATGTCTGTTTAATCATCTCCAAGTGACG
CATTCTGAAGAATCTACAAGAAGAAGCCTGTTTTGTAGGTTTAGTATTGTGCAGAGGAGAAAAAGATCGAAGAGAGTTTCCAGGTACAAGAAGATTCTTC
CTCGATTCTCTGTTTCTTCTTCTTCGTGTACTATGTTTCCTGTTTCTGTGGATGATGGAGGATTATTAGAAGTCGCTGAGTCTCTGATTCTCTTGTCCAT
GAGTGGTGGAAAGTTCGTGAATGGTTTGGAACATTTTGGTAAAGCTCTGGGTTCGACTCAGAGAAAATTTGAAGACGGGTTATTGAGAAATGAGCAGAGA
CTTGTTGGTGAAGCTCTGGATTCGAATCCAGAGGTGAATTTGAGAAAAGATGGAGACTTTGAATATGGGCTTTTGAGTAATGAGCAGAAGAAACTTGTGG
GGATAAGCAGAGCATCTGTTGGAACATCGAAAGAGCTTTCGGGTTACTTGGCAAACAAGAAGGGCAGAGAAGATGATGAATTGGGTCAGCAGAAACAGGC
TGGAGCAAGAATACTTAGAGAAGAAACTGATAACGAACAGAAACTTGTTCGTCAAGAAACTGCATTTGAAGATTCAGTATCAGGATTTGAGATGAATATA
GAGCATCGGTGTGGGCTCTGTCACAAGGTTTTCTCGACTTATCAAACTCTTGGTGGTCATCAGACGTTCCATAGAATGAGAAACAAATCCAAAAGTCAAA
CCAAGAGATGCAGAGAAGAATCGATAGAAGCTGAAGCTGGGATTAATAATGGTTCAGTGACGTTGACAATAAGTGCAGAGTTTGCTGAAGGCTGTTTAGG
TCAGTACATGCTT

>IE_07D07_translated
MEEQQEFEKPIFEFRPKKLRTSRVSRNLMKKTGFRESMNHYEELSCNYGLRENPKKTQKSLLDHRFCTRRRRNKKILIRCKECGKGFLYEKCLFNHLQVT
HSEESTRRSLFCRFSIVQRRKRSKRVSRYKKILPRFSVSSSSCTMFPVSVDDGGLLEVAESLILLSMSGGKFVNGLEHFGKALGSTQRKFEDGLLRNEQR
LVGEALDSNPEVNLRKDGDFEYGLLSNEQKKLVGISRASVGTSKELSGYLANKKGREDDELGQQKQAGARILREETDNEQKLVRQETAFEDSVSGFEMNI
EHRCGLCHKVFSTYQTLGGHQTFHRMRNKSKSQTKRCREESIEAEAGINNGSVTLTISAEFAEGCLGQYML
Alignment ◆CDS（TAIRver.9）_without_stop_codonとのalignment◆

100%一致しました。

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