◆◆◆IE_07D08 detail◆◆◆

Code name	IE_07D08
Locus	AT3G05380
Template	IT_07D08 (ABRC_168)
Donor Vector	pDONR207
Forward primer	IPF_07D08 (ggggacaagtttgtacaaaaaagcaggcttcATGGCACCAGTTAGGAAGTCGAGGAGT)
Reverse primer	IPR_07D08 (ggggaccactttgtacaagaaagctgggttAGTTGGTACAAGGGACATGATTTGAGTCTT)
Alignment with template seq.	1st	配列解析が行えなかったサンプルです。	2nd	アミノ酸配列85.90%一致
Alignment with TAIR7CDS	1st	-	2nd	アミノ酸配列85.90%一致
comment	1st:(Sequence解析まで行ったが、読み直す必要がある?), 2nd:70%, 3nt_ins
sequence	>IE_07D08_3156bp ATGGCACCAGTTAGGAAGTCGAGGAGTGTGAACAAGCGTTTCACCAATGAAACTTCCCCAAGAAAAGATGCTGGGAAATCGAAAAAAAATAAGCTACGTA AGAAGAAATTGTCTGACAAGCTGGGACCTCAGTGGACCAGATTAGAACTTGAGCGTTTCTATGATGCTTACCGCAAGCACGGACAAGAGTGGAGAAGGGT GGCTGCTGCAATTCGGAATAGCAGGTCTGTTGACATGGTGGAAGCTCTATTTAATATGAATCGGGCGTATTTATCTCTCCCCGAGGGAACTGCCTCTGTA GCTGGCCTTATTGCGATGATGACAGATCATTACAGTGTCATGGAAGGGAGTGGCAGTGAAGGAGAAGGCCATGATGCTTCAGAAGTACCAAGGAAACAAC AAAAGCGCAAACGTGCTAAACCTCAACGTAGCGATTCTCCAGAGGAAGTGGATATACAACAGTCAATTGGTTCACCAGATGGATGCCTCACGTTTTTGAA GCAAGCACGAGCTAATGGAACTCAGCGACATGCCACTGGAAAACGTACACCACGGGTTCCTGTACAAACTTCATTTATGAGGGATGATAGAGAAGGCTCT ACTCCACCAAATAAAAGAGCCAGGAAGCAATTCGATGCCAATGATGATGTAGCACATTTTTTAGCGTTAGCATTAACAGATGCATCGAGAAGGGGAGGGT CTCCAAAAGTTTCTGAATCACCAAATAGAAGAACAGAACTTAGCGATAGCTCACCGATAAAGAGCTGGGGGAAAATGTCGCGAACCAGAAAATCTCAGTC TAAGCACTGTGGCAGCTCCATTTTCGAGGAGTGGATGGAAAGTAGCCGAGAAAGGAAACTTGACTCTGATAAAGATACTACCTTGTTGATGGATATGGAA AGGGCTGGCGAAATGGAGGCTCCGCGGAAGGGGAAAAGAGTCTACAAGAAAAGAGTGAAAGTCGAAGAAGCAGAATGTAATGATTCTGATGACAATGGAG AAGCATGCAGTGCCACACAAGGGCTCAGAAGTAAATCACAAAGACGAAAGGCTGCTATTGAAGCCTCAAGAGAGAAATACTCACCGCGCAGCCCAAAGAA AAGAGATGACAAACATACTTCCGGAGCTTTTGATGCCCTGCAAGCATTGGCCGAATTGTCAGCTTCAATGCTTCCAGCAAATTTGATGGAGTCAGAATTA TCTGCTCAGTTGAAGGAAGAAAGAACAGAATACGACATGGACGAGAAATCTAGCACACCAGAAGCTACTTCCACAAGCAGTCATGGGGAAAAAGCAAATG TAGAGCCAGATGATAGTCTCCTTCATGCAATTTCTTCTGTTGAGAATGCTAACAAGAGAAAGTCAAAACCTTCAAGGCTGGTATCCACTGATTGTGATGA TGTTCCCACAGGGAAGCTACAACCACAAACTAGTGGCAGTTTAAGAAAACGTAAACCAAAGGTACTAGGGGATGAAGCTCCAGCAGAGTTTAGTCAGAAC AAATCCATAAACAAGAAGGAATTGCCTCAAGATGAGAATAATATGAAGTCTTTGGTTAAAACAAAACGCGCTGGTCAAGTTCCGGCTCAGTCAAAACAGA TGAAAACTGTTAAGGCGTTGGAGGAATCTGCTATAACAAGTGATAAGAAAAGACCTGGGATGGATATAGTAGCGTCACCTAAACAAGTTTCTGACTCGGG TCCAACCAGTTTATCGCAGAAACCTCCAAACAGGCGAAAGAAGAGTCTGCAGAAAAGCTTACAAGAAAAGGCTAAATCTTCTGAAACCACTCATAAAGCT GCACGTAGTTCCAGATCTCTTTCAGAACAAGAGTTGTTGTTAAAGGATAAGCTTGCTACTTCTCTGTCGTTTCCCTTTGCACGTCGAAGGTGCATATTTG AATGGTTTTATAGTGCTATCGACCATCCCTGGTTTTCAAAGATGGAGTTTGTCGATTACCTAAATCACGTGGGGCTTGGTCACATTCCAAGACTTACTCG TCTTGAATGGAGCGTCATTAAAAGTTCTCTTGGTAGACCACGAAGATTCTCTGAGAGATTTTTACATGAAGAGCGGGAGAAACTTAAACAGTACCGCGAA TCTGTGCGAAAGCATTATACAGAGCTTCGAACGGGTGCTAGGGAGGGGCTTCCTACAGATTTGGCTCGGCCATTAGCAGTTGGGAACAGAGTCATTGCCA TCCATCCCAAAACACGGGAGATTCATGATGGAAAAATTCTCACTGTTGATCATAACAAATGCAATGTTCTCTTCGATGACTTGGGCGTTGAGTTGGTTAT GGACATTGATTGCATGCCTTTAAATCCACTGGAATACATGCCAGAGGGTCTAAGGAGGCAAATTGATAAGTGCTTGTCTATGAAGAAAGAAGCACAGCTA AGCGGGAATACAAACCTTGGTGTGTCTGTGCTATTCCCTCCTTGCGGACTTGAAAATGTCAGCTTTTCCATGAATCCTCCTCTGAATCAGGGTGATATGA TTGCTCCCATTCTGCATGGTAAAGTATCAAGCAACACTAGTAGTCCACGGCAGACTAATCATTCATATATCACAACTTATAACAAAGCAAAAGAAGCTGA GATTCAACGGGCACAAGCGCTACAGCATGCTTTAGATGAAAAGGAAATGGAGCCAGAGATGCTAGAAATTGTCAAGGGTTCAAAAACAAGAGCGCAAGCA ATGGTGGATGCAGCTATAAAGGCTGCATCATCTGTGAAGGAAGGGGAAGATGTGAACACAATGATCCAAGAAGCCTTAGAGTTGGTTGGCAAAAATCAGC TATTACGCAGCTCTATGGTCAAACATCACGAGCATGTAAATGGCAGTATAGAGCATCATCACAACCCATCTCCTTCAAATGGATCAGAGCCTGTGGCTAA CAACGATTTAAACTCACAAGATGGTTCAGAGAAAAACGCTCAAATGCCTTCAGAGCTTATCACCTCCTGTGTTGCCACTTGGCTCATGATTCAGATGTGC ACGGAGAGACAGTACCCTCCAGCTGATGTCGCACAGCTTATAGACGCAGCAGTCACAAGCTTGCAGCCACGATGCCCACAGAACCTACCGATCTACAGAG AAATCCAAACGTGCATGGGACGGATCAAGACTCAAATCATGTCCCTTGTACCAACT >IE_07D08_translated MAPVRKSRSVNKRFTNETSPRKDAGKSKKNKLRKKKLSDKLGPQWTRLELERFYDAYRKHGQEWRRVAAAIRNSRSVDMVEALFNMNRAYLSLPEGTASV AGLIAMMTDHYSVMEGSGSEGEGHDASEVPRKQQKRKRAKPQRSDSPEEVDIQQSIGSPDGCLTFLKQARANGTQRHATGKRTPRVPVQTSFMRDDREGS TPPNKRARKQFDANDDVAHFLALALTDASRRGGSPKVSESPNRRTELSDSSPIKSWGKMSRTRKSQSKHCGSSIFEEWMESSRERKLDSDKDTTLLMDME RAGEMEAPRKGKRVYKKRVKVEEAECNDSDDNGEACSATQGLRSKSQRRKAAIEASREKYSPRSPKKRDDKHTSGAFDALQALAELSASMLPANLMESEL SAQLKEERTEYDMDEKSSTPEATSTSSHGEKANVEPDDSLLHAISSVENANKRKSKPSRLVSTDCDDVPTGKLQPQTSGSLRKRKPKVLGDEAPAEFSQN KSINKKELPQDENNMKSLVKTKRAGQVPAQSKQMKTVKALEESAITSDKKRPGMDIVASPKQVSDSGPTSLSQKPPNRRKKSLQKSLQEKAKSSETTHKA ARSSRSLSEQELLLKDKLATSLSFPFARRRCIFEWFYSAIDHPWFSKMEFVDYLNHVGLGHIPRLTRLEWSVIKSSLGRPRRFSERFLHEEREKLKQYRE SVRKHYTELRTGAREGLPTDLARPLAVGNRVIAIHPKTREIHDGKILTVDHNKCNVLFDDLGVELVMDIDCMPLNPLEYMPEGLRRQIDKCLSMKKEAQL SGNTNLGVSVLFPPCGLENVSFSMNPPLNQGDMIAPILHGKVSSNTSSPRQTNHSYITTYNKAKEAEIQRAQALQHALDEKEMEPEMLEIVKGSKTRAQA MVDAAIKAASSVKEGEDVNTMIQEALELVGKNQLLRSSMVKHHEHVNGSIEHHHNPSPSNGSEPVANNDLNSQDGSEKNAQMPSELITSCVATWLMIQMC TERQYPPADVAQLIDAAVTSLQPRCPQNLPIYREIQTCMGRIKTQIMSLVPT
Alignment	◆CDS（TAIRver.9）_without_stop_codonとのalignment◆ 3153/3156 (99.9%)（アミノ酸配列で1051/1052 (99.9%)）一致しました。 10 20 30 40 50 60 70 80 90 AT3G05 ATGGCACCAGTTAGGAAGTCGAGGAGTGTGAACAAGCGTTTCACCAATGAAACTTCCCCAAGAAAAGATGCTGGGAAATCGAAAAAAAATAAGCTACGT- ::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_07D ATGGCACCAGTTAGGAAGTCGAGGAGTGTGAACAAGCGTTTCACCAATGAAACTTCCCCAAGAAAAGATGCTGGGAAATCGAAAAAAAATAAGCTACGTA 10 20 30 40 50 60 70 80 90 100 100 110 120 130 140 150 160 170 180 190 AT3G05 --AAGAAATTGTCTGACAAGCTGGGACCTCAGTGGACCAGATTAGAACTTGAGCGTTTCTATGATGCTTACCGCAAGCACGGACAAGAGTGGAGAAGGGT :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_07D AGAAGAAATTGTCTGACAAGCTGGGACCTCAGTGGACCAGATTAGAACTTGAGCGTTTCTATGATGCTTACCGCAAGCACGGACAAGAGTGGAGAAGGGT 110 120 130 140 150 160 170 180 190 200 200 210 220 230 240 250 260 270 280 290 AT3G05 GGCTGCTGCAATTCGGAATAGCAGGTCTGTTGACATGGTGGAAGCTCTATTTAATATGAATCGGGCGTATTTATCTCTCCCCGAGGGAACTGCCTCTGTA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_07D GGCTGCTGCAATTCGGAATAGCAGGTCTGTTGACATGGTGGAAGCTCTATTTAATATGAATCGGGCGTATTTATCTCTCCCCGAGGGAACTGCCTCTGTA 210 220 230 240 250 260 270 280 290 300 300 310 320 330 340 350 360 370 380 390 AT3G05 GCTGGCCTTATTGCGATGATGACAGATCATTACAGTGTCATGGAAGGGAGTGGCAGTGAAGGAGAAGGCCATGATGCTTCAGAAGTACCAAGGAAACAAC :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_07D GCTGGCCTTATTGCGATGATGACAGATCATTACAGTGTCATGGAAGGGAGTGGCAGTGAAGGAGAAGGCCATGATGCTTCAGAAGTACCAAGGAAACAAC 310 320 330 340 350 360 370 380 390 400 400 410 420 430 440 450 460 470 480 490 AT3G05 AAAAGCGCAAACGTGCTAAACCTCAACGTAGCGATTCTCCAGAGGAAGTGGATATACAACAGTCAATTGGTTCACCAGATGGATGCCTCACGTTTTTGAA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_07D AAAAGCGCAAACGTGCTAAACCTCAACGTAGCGATTCTCCAGAGGAAGTGGATATACAACAGTCAATTGGTTCACCAGATGGATGCCTCACGTTTTTGAA 410 420 430 440 450 460 470 480 490 500 500 510 520 530 540 550 560 570 580 590 AT3G05 GCAAGCACGAGCTAATGGAACTCAGCGACATGCCACTGGAAAACGTACACCACGGGTTCCTGTACAAACTTCATTTATGAGGGATGATAGAGAAGGCTCT :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_07D GCAAGCACGAGCTAATGGAACTCAGCGACATGCCACTGGAAAACGTACACCACGGGTTCCTGTACAAACTTCATTTATGAGGGATGATAGAGAAGGCTCT 510 520 530 540 550 560 570 580 590 600 600 610 620 630 640 650 660 670 680 690 AT3G05 ACTCCACCAAATAAAAGAGCCAGGAAGCAATTCGATGCCAATGATGATGTAGCACATTTTTTAGCGTTAGCATTAACAGATGCATCGAGAAGGGGAGGGT :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_07D ACTCCACCAAATAAAAGAGCCAGGAAGCAATTCGATGCCAATGATGATGTAGCACATTTTTTAGCGTTAGCATTAACAGATGCATCGAGAAGGGGAGGGT 610 620 630 640 650 660 670 680 690 700 700 710 720 730 740 750 760 770 780 790 AT3G05 CTCCAAAAGTTTCTGAATCACCAAATAGAAGAACAGAACTTAGCGATAGCTCACCGATAAAGAGCTGGGGGAAAATGTCGCGAACCAGAAAATCTCAGTC :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_07D CTCCAAAAGTTTCTGAATCACCAAATAGAAGAACAGAACTTAGCGATAGCTCACCGATAAAGAGCTGGGGGAAAATGTCGCGAACCAGAAAATCTCAGTC 710 720 730 740 750 760 770 780 790 800 800 810 820 830 840 850 860 870 880 890 AT3G05 TAAGCACTGTGGCAGCTCCATTTTCGAGGAGTGGATGGAAAGTAGCCGAGAAAGGAAACTTGACTCTGATAAAGATACTACCTTGTTGATGGATATGGAA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_07D TAAGCACTGTGGCAGCTCCATTTTCGAGGAGTGGATGGAAAGTAGCCGAGAAAGGAAACTTGACTCTGATAAAGATACTACCTTGTTGATGGATATGGAA 810 820 830 840 850 860 870 880 890 900 900 910 920 930 940 950 960 970 980 990 AT3G05 AGGGCTGGCGAAATGGAGGCTCCGCGGAAGGGGAAAAGAGTCTACAAGAAAAGAGTGAAAGTCGAAGAAGCAGAATGTAATGATTCTGATGACAATGGAG :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_07D AGGGCTGGCGAAATGGAGGCTCCGCGGAAGGGGAAAAGAGTCTACAAGAAAAGAGTGAAAGTCGAAGAAGCAGAATGTAATGATTCTGATGACAATGGAG 910 920 930 940 950 960 970 980 990 1000 1000 1010 1020 1030 1040 1050 1060 1070 1080 1090 AT3G05 AAGCATGCAGTGCCACACAAGGGCTCAGAAGTAAATCACAAAGACGAAAGGCTGCTATTGAAGCCTCAAGAGAGAAATACTCACCGCGCAGCCCAAAGAA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_07D AAGCATGCAGTGCCACACAAGGGCTCAGAAGTAAATCACAAAGACGAAAGGCTGCTATTGAAGCCTCAAGAGAGAAATACTCACCGCGCAGCCCAAAGAA 1010 1020 1030 1040 1050 1060 1070 1080 1090 1100 1100 1110 1120 1130 1140 1150 1160 1170 1180 1190 AT3G05 AAGAGATGACAAACATACTTCCGGAGCTTTTGATGCCCTGCAAGCATTGGCCGAATTGTCAGCTTCAATGCTTCCAGCAAATTTGATGGAGTCAGAATTA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_07D AAGAGATGACAAACATACTTCCGGAGCTTTTGATGCCCTGCAAGCATTGGCCGAATTGTCAGCTTCAATGCTTCCAGCAAATTTGATGGAGTCAGAATTA 1110 1120 1130 1140 1150 1160 1170 1180 1190 1200 1200 1210 1220 1230 1240 1250 1260 1270 1280 1290 AT3G05 TCTGCTCAGTTGAAGGAAGAAAGAACAGAATACGACATGGACGAGAAATCTAGCACACCAGAAGCTACTTCCACAAGCAGTCATGGGGAAAAAGCAAATG :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_07D TCTGCTCAGTTGAAGGAAGAAAGAACAGAATACGACATGGACGAGAAATCTAGCACACCAGAAGCTACTTCCACAAGCAGTCATGGGGAAAAAGCAAATG 1210 1220 1230 1240 1250 1260 1270 1280 1290 1300 1300 1310 1320 1330 1340 1350 1360 1370 1380 1390 AT3G05 TAGAGCCAGATGATAGTCTCCTTCATGCAATTTCTTCTGTTGAGAATGCTAACAAGAGAAAGTCAAAACCTTCAAGGCTGGTATCCACTGATTGTGATGA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_07D TAGAGCCAGATGATAGTCTCCTTCATGCAATTTCTTCTGTTGAGAATGCTAACAAGAGAAAGTCAAAACCTTCAAGGCTGGTATCCACTGATTGTGATGA 1310 1320 1330 1340 1350 1360 1370 1380 1390 1400 1400 1410 1420 1430 1440 1450 1460 1470 1480 1490 AT3G05 TGTTCCCACAGGGAAGCTACAACCACAAACTAGTGGCAGTTTAAGAAAACGTAAACCAAAGGTACTAGGGGATGAAGCTCCAGCAGAGTTTAGTCAGAAC :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_07D TGTTCCCACAGGGAAGCTACAACCACAAACTAGTGGCAGTTTAAGAAAACGTAAACCAAAGGTACTAGGGGATGAAGCTCCAGCAGAGTTTAGTCAGAAC 1410 1420 1430 1440 1450 1460 1470 1480 1490 1500 1500 1510 1520 1530 1540 1550 1560 1570 1580 1590 AT3G05 AAATCCATAAACAAGAAGGAATTGCCTCAAGATGAGAATAATATGAAGTCTTTGGTTAAAACAAAACGCGCTGGTCAAGTTCCGGCTCAGTCAAAACAGA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_07D AAATCCATAAACAAGAAGGAATTGCCTCAAGATGAGAATAATATGAAGTCTTTGGTTAAAACAAAACGCGCTGGTCAAGTTCCGGCTCAGTCAAAACAGA 1510 1520 1530 1540 1550 1560 1570 1580 1590 1600 1600 1610 1620 1630 1640 1650 1660 1670 1680 1690 AT3G05 TGAAAACTGTTAAGGCGTTGGAGGAATCTGCTATAACAAGTGATAAGAAAAGACCTGGGATGGATATAGTAGCGTCACCTAAACAAGTTTCTGACTCGGG :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_07D TGAAAACTGTTAAGGCGTTGGAGGAATCTGCTATAACAAGTGATAAGAAAAGACCTGGGATGGATATAGTAGCGTCACCTAAACAAGTTTCTGACTCGGG 1610 1620 1630 1640 1650 1660 1670 1680 1690 1700 1700 1710 1720 1730 1740 1750 1760 1770 1780 1790 AT3G05 TCCAACCAGTTTATCGCAGAAACCTCCAAACAGGCGAAAGAAGAGTCTGCAGAAAAGCTTACAAGAAAAGGCTAAATCTTCTGAAACCACTCATAAAGCT :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_07D TCCAACCAGTTTATCGCAGAAACCTCCAAACAGGCGAAAGAAGAGTCTGCAGAAAAGCTTACAAGAAAAGGCTAAATCTTCTGAAACCACTCATAAAGCT 1710 1720 1730 1740 1750 1760 1770 1780 1790 1800 1800 1810 1820 1830 1840 1850 1860 1870 1880 1890 AT3G05 GCACGTAGTTCCAGATCTCTTTCAGAACAAGAGTTGTTGTTAAAGGATAAGCTTGCTACTTCTCTGTCGTTTCCCTTTGCACGTCGAAGGTGCATATTTG :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_07D GCACGTAGTTCCAGATCTCTTTCAGAACAAGAGTTGTTGTTAAAGGATAAGCTTGCTACTTCTCTGTCGTTTCCCTTTGCACGTCGAAGGTGCATATTTG 1810 1820 1830 1840 1850 1860 1870 1880 1890 1900 1900 1910 1920 1930 1940 1950 1960 1970 1980 1990 AT3G05 AATGGTTTTATAGTGCTATCGACCATCCCTGGTTTTCAAAGATGGAGTTTGTCGATTACCTAAATCACGTGGGGCTTGGTCACATTCCAAGACTTACTCG :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_07D AATGGTTTTATAGTGCTATCGACCATCCCTGGTTTTCAAAGATGGAGTTTGTCGATTACCTAAATCACGTGGGGCTTGGTCACATTCCAAGACTTACTCG 1910 1920 1930 1940 1950 1960 1970 1980 1990 2000 2000 2010 2020 2030 2040 2050 2060 2070 2080 2090 AT3G05 TCTTGAATGGAGCGTCATTAAAAGTTCTCTTGGTAGACCACGAAGATTCTCTGAGAGATTTTTACATGAAGAGCGGGAGAAACTTAAACAGTACCGCGAA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_07D TCTTGAATGGAGCGTCATTAAAAGTTCTCTTGGTAGACCACGAAGATTCTCTGAGAGATTTTTACATGAAGAGCGGGAGAAACTTAAACAGTACCGCGAA 2010 2020 2030 2040 2050 2060 2070 2080 2090 2100 2100 2110 2120 2130 2140 2150 2160 2170 2180 2190 AT3G05 TCTGTGCGAAAGCATTATACAGAGCTTCGAACGGGTGCTAGGGAGGGGCTTCCTACAGATTTGGCTCGGCCATTAGCAGTTGGGAACAGAGTCATTGCCA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_07D TCTGTGCGAAAGCATTATACAGAGCTTCGAACGGGTGCTAGGGAGGGGCTTCCTACAGATTTGGCTCGGCCATTAGCAGTTGGGAACAGAGTCATTGCCA 2110 2120 2130 2140 2150 2160 2170 2180 2190 2200 2200 2210 2220 2230 2240 2250 2260 2270 2280 2290 AT3G05 TCCATCCCAAAACACGGGAGATTCATGATGGAAAAATTCTCACTGTTGATCATAACAAATGCAATGTTCTCTTCGATGACTTGGGCGTTGAGTTGGTTAT :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_07D TCCATCCCAAAACACGGGAGATTCATGATGGAAAAATTCTCACTGTTGATCATAACAAATGCAATGTTCTCTTCGATGACTTGGGCGTTGAGTTGGTTAT 2210 2220 2230 2240 2250 2260 2270 2280 2290 2300 2300 2310 2320 2330 2340 2350 2360 2370 2380 2390 AT3G05 GGACATTGATTGCATGCCTTTAAATCCACTGGAATACATGCCAGAGGGTCTAAGGAGGCAAATTGATAAGTGCTTGTCTATGAAGAAAGAAGCACAGCTA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_07D GGACATTGATTGCATGCCTTTAAATCCACTGGAATACATGCCAGAGGGTCTAAGGAGGCAAATTGATAAGTGCTTGTCTATGAAGAAAGAAGCACAGCTA 2310 2320 2330 2340 2350 2360 2370 2380 2390 2400 2400 2410 2420 2430 2440 2450 2460 2470 2480 2490 AT3G05 AGCGGGAATACAAACCTTGGTGTGTCTGTGCTATTCCCTCCTTGCGGACTTGAAAATGTCAGCTTTTCCATGAATCCTCCTCTGAATCAGGGTGATATGA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_07D AGCGGGAATACAAACCTTGGTGTGTCTGTGCTATTCCCTCCTTGCGGACTTGAAAATGTCAGCTTTTCCATGAATCCTCCTCTGAATCAGGGTGATATGA 2410 2420 2430 2440 2450 2460 2470 2480 2490 2500 2500 2510 2520 2530 2540 2550 2560 2570 2580 2590 AT3G05 TTGCTCCCATTCTGCATGGTAAAGTATCAAGCAACACTAGTAGTCCACGGCAGACTAATCATTCATATATCACAACTTATAACAAAGCAAAAGAAGCTGA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_07D TTGCTCCCATTCTGCATGGTAAAGTATCAAGCAACACTAGTAGTCCACGGCAGACTAATCATTCATATATCACAACTTATAACAAAGCAAAAGAAGCTGA 2510 2520 2530 2540 2550 2560 2570 2580 2590 2600 2600 2610 2620 2630 2640 2650 2660 2670 2680 2690 AT3G05 GATTCAACGGGCACAAGCGCTACAGCATGCTTTAGATGAAAAGGAAATGGAGCCAGAGATGCTAGAAATTGTCAAGGGTTCAAAAACAAGAGCGCAAGCA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_07D GATTCAACGGGCACAAGCGCTACAGCATGCTTTAGATGAAAAGGAAATGGAGCCAGAGATGCTAGAAATTGTCAAGGGTTCAAAAACAAGAGCGCAAGCA 2610 2620 2630 2640 2650 2660 2670 2680 2690 2700 2700 2710 2720 2730 2740 2750 2760 2770 2780 2790 AT3G05 ATGGTGGATGCAGCTATAAAGGCTGCATCATCTGTGAAGGAAGGGGAAGATGTGAACACAATGATCCAAGAAGCCTTAGAGTTGGTTGGCAAAAATCAGC :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_07D ATGGTGGATGCAGCTATAAAGGCTGCATCATCTGTGAAGGAAGGGGAAGATGTGAACACAATGATCCAAGAAGCCTTAGAGTTGGTTGGCAAAAATCAGC 2710 2720 2730 2740 2750 2760 2770 2780 2790 2800 2800 2810 2820 2830 2840 2850 2860 2870 2880 2890 AT3G05 TATTACGCAGCTCTATGGTCAAACATCACGAGCATGTAAATGGCAGTATAGAGCATCATCACAACCCATCTCCTTCAAATGGATCAGAGCCTGTGGCTAA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_07D TATTACGCAGCTCTATGGTCAAACATCACGAGCATGTAAATGGCAGTATAGAGCATCATCACAACCCATCTCCTTCAAATGGATCAGAGCCTGTGGCTAA 2810 2820 2830 2840 2850 2860 2870 2880 2890 2900 2900 2910 2920 2930 2940 2950 2960 2970 2980 2990 AT3G05 CAACGATTTAAACTCACAAGATGGTTCAGAGAAAAACGCTCAAATGCCTTCAGAGCTTATCACCTCCTGTGTTGCCACTTGGCTCATGATTCAGATGTGC :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_07D CAACGATTTAAACTCACAAGATGGTTCAGAGAAAAACGCTCAAATGCCTTCAGAGCTTATCACCTCCTGTGTTGCCACTTGGCTCATGATTCAGATGTGC 2910 2920 2930 2940 2950 2960 2970 2980 2990 3000 3000 3010 3020 3030 3040 3050 3060 3070 3080 3090 AT3G05 ACGGAGAGACAGTACCCTCCAGCTGATGTCGCACAGCTTATAGACGCAGCAGTCACAAGCTTGCAGCCACGATGCCCACAGAACCTACCGATCTACAGAG :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_07D ACGGAGAGACAGTACCCTCCAGCTGATGTCGCACAGCTTATAGACGCAGCAGTCACAAGCTTGCAGCCACGATGCCCACAGAACCTACCGATCTACAGAG 3010 3020 3030 3040 3050 3060 3070 3080 3090 3100 3100 3110 3120 3130 3140 3150 AT3G05 AAATCCAAACGTGCATGGGACGGATCAAGACTCAAATCATGTCCCTTGTACCAACT :::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_07D AAATCCAAACGTGCATGGGACGGATCAAGACTCAAATCATGTCCCTTGTACCAACT 3110 3120 3130 3140 3150 10 20 30 40 50 60 70 80 90 AT3G05 MAPVRKSRSVNKRFTNETSPRKDAGKSKKNKLR-KKLSDKLGPQWTRLELERFYDAYRKHGQEWRRVAAAIRNSRSVDMVEALFNMNRAYLSLPEGTASV ::::::::::::::::::::::::::::::::: :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_07D MAPVRKSRSVNKRFTNETSPRKDAGKSKKNKLRKKKLSDKLGPQWTRLELERFYDAYRKHGQEWRRVAAAIRNSRSVDMVEALFNMNRAYLSLPEGTASV 10 20 30 40 50 60 70 80 90 100 100 110 120 130 140 150 160 170 180 190 AT3G05 AGLIAMMTDHYSVMEGSGSEGEGHDASEVPRKQQKRKRAKPQRSDSPEEVDIQQSIGSPDGCLTFLKQARANGTQRHATGKRTPRVPVQTSFMRDDREGS :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_07D AGLIAMMTDHYSVMEGSGSEGEGHDASEVPRKQQKRKRAKPQRSDSPEEVDIQQSIGSPDGCLTFLKQARANGTQRHATGKRTPRVPVQTSFMRDDREGS 110 120 130 140 150 160 170 180 190 200 200 210 220 230 240 250 260 270 280 290 AT3G05 TPPNKRARKQFDANDDVAHFLALALTDASRRGGSPKVSESPNRRTELSDSSPIKSWGKMSRTRKSQSKHCGSSIFEEWMESSRERKLDSDKDTTLLMDME :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_07D TPPNKRARKQFDANDDVAHFLALALTDASRRGGSPKVSESPNRRTELSDSSPIKSWGKMSRTRKSQSKHCGSSIFEEWMESSRERKLDSDKDTTLLMDME 210 220 230 240 250 260 270 280 290 300 300 310 320 330 340 350 360 370 380 390 AT3G05 RAGEMEAPRKGKRVYKKRVKVEEAECNDSDDNGEACSATQGLRSKSQRRKAAIEASREKYSPRSPKKRDDKHTSGAFDALQALAELSASMLPANLMESEL :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_07D RAGEMEAPRKGKRVYKKRVKVEEAECNDSDDNGEACSATQGLRSKSQRRKAAIEASREKYSPRSPKKRDDKHTSGAFDALQALAELSASMLPANLMESEL 310 320 330 340 350 360 370 380 390 400 400 410 420 430 440 450 460 470 480 490 AT3G05 SAQLKEERTEYDMDEKSSTPEATSTSSHGEKANVEPDDSLLHAISSVENANKRKSKPSRLVSTDCDDVPTGKLQPQTSGSLRKRKPKVLGDEAPAEFSQN :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_07D SAQLKEERTEYDMDEKSSTPEATSTSSHGEKANVEPDDSLLHAISSVENANKRKSKPSRLVSTDCDDVPTGKLQPQTSGSLRKRKPKVLGDEAPAEFSQN 410 420 430 440 450 460 470 480 490 500 500 510 520 530 540 550 560 570 580 590 AT3G05 KSINKKELPQDENNMKSLVKTKRAGQVPAQSKQMKTVKALEESAITSDKKRPGMDIVASPKQVSDSGPTSLSQKPPNRRKKSLQKSLQEKAKSSETTHKA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_07D KSINKKELPQDENNMKSLVKTKRAGQVPAQSKQMKTVKALEESAITSDKKRPGMDIVASPKQVSDSGPTSLSQKPPNRRKKSLQKSLQEKAKSSETTHKA 510 520 530 540 550 560 570 580 590 600 600 610 620 630 640 650 660 670 680 690 AT3G05 ARSSRSLSEQELLLKDKLATSLSFPFARRRCIFEWFYSAIDHPWFSKMEFVDYLNHVGLGHIPRLTRLEWSVIKSSLGRPRRFSERFLHEEREKLKQYRE :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_07D ARSSRSLSEQELLLKDKLATSLSFPFARRRCIFEWFYSAIDHPWFSKMEFVDYLNHVGLGHIPRLTRLEWSVIKSSLGRPRRFSERFLHEEREKLKQYRE 610 620 630 640 650 660 670 680 690 700 700 710 720 730 740 750 760 770 780 790 AT3G05 SVRKHYTELRTGAREGLPTDLARPLAVGNRVIAIHPKTREIHDGKILTVDHNKCNVLFDDLGVELVMDIDCMPLNPLEYMPEGLRRQIDKCLSMKKEAQL :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_07D SVRKHYTELRTGAREGLPTDLARPLAVGNRVIAIHPKTREIHDGKILTVDHNKCNVLFDDLGVELVMDIDCMPLNPLEYMPEGLRRQIDKCLSMKKEAQL 710 720 730 740 750 760 770 780 790 800 800 810 820 830 840 850 860 870 880 890 AT3G05 SGNTNLGVSVLFPPCGLENVSFSMNPPLNQGDMIAPILHGKVSSNTSSPRQTNHSYITTYNKAKEAEIQRAQALQHALDEKEMEPEMLEIVKGSKTRAQA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_07D SGNTNLGVSVLFPPCGLENVSFSMNPPLNQGDMIAPILHGKVSSNTSSPRQTNHSYITTYNKAKEAEIQRAQALQHALDEKEMEPEMLEIVKGSKTRAQA 810 820 830 840 850 860 870 880 890 900 900 910 920 930 940 950 960 970 980 990 AT3G05 MVDAAIKAASSVKEGEDVNTMIQEALELVGKNQLLRSSMVKHHEHVNGSIEHHHNPSPSNGSEPVANNDLNSQDGSEKNAQMPSELITSCVATWLMIQMC :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_07D MVDAAIKAASSVKEGEDVNTMIQEALELVGKNQLLRSSMVKHHEHVNGSIEHHHNPSPSNGSEPVANNDLNSQDGSEKNAQMPSELITSCVATWLMIQMC 910 920 930 940 950 960 970 980 990 1000 1000 1010 1020 1030 1040 1050 AT3G05 TERQYPPADVAQLIDAAVTSLQPRCPQNLPIYREIQTCMGRIKTQIMSLVPT :::::::::::::::::::::::::::::::::::::::::::::::::::: IE_07D TERQYPPADVAQLIDAAVTSLQPRCPQNLPIYREIQTCMGRIKTQIMSLVPT 1010 1020 1030 1040 1050

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