âââIE_07E02 detailâââ

Code name	IE_07E02
Locus	AT4G11400
Template	IT_07E02 (ABRC_182)
Donor Vector	pDONR207
Forward primer	IPF_07E02 (ggggacaagtttgtacaaaaaagcaggcttcATGGGAAGTTTTAATGATACTTCATGT)
Reverse primer	IPR_07E02 (ggggaccactttgtacaagaaagctgggttGTAGTCCTCACACTGACGATTCTGAGCACA)
Alignment with template seq.	1st	éåè§£æãè¡ããªãã£ããµã³ãã«ã§ãã	2nd	ã¢ããé¸éå100.00%ä¸è´
Alignment with TAIR7CDS	1st	-	2nd	ã¢ããé¸éå100.00%ä¸è´
comment	1st:(Sequenceè§£æã¾ã§è¡ã£ãããèªã¿ç´ãå¿è¦ããã), 2nd:70%, Walkingprimerを設計し解析を行ったが, インサート内部でピークが急激に下がる現象が見られた為, 解析不能となった。
sequence	>IE_07E02_1719bp ATGGGAAGTTTTAATGATACTTCATGTTCATATGTTGATGTTGAGATTAAGTATGTTGATGAATGTGAGGAGAGGTTAAGGAGATTGTTTGATCAAGCTT TGTTGGTTTTTCTTGAGGAAGAGGGTAGTATAAAGCCTCTCCCTGCAGTTATAGGTGATGGCAAAAATGTTGATTTGTTTAAGCTGTTTGTATTGGTGAG GGAGAGGGAAGGGTTTGATACTGTGTCTAGGAAAAGGTTGTGGGAAGTAGTGGCTGAGAAGTTAGGCTTTGATTGTTCACTGGTTCCTTCTTTGATATTG ATTTACTTGAAGTATTTGAACCGGATGGAGAAATGGGCGGTGGAGGAATCTAGGATTGTGAATTGGGATAATAAGGATAGTGAAAAGAAAGGGTGTTATA GTGGTATGTTGCATGAGTTAGGGAATGGTTTTAAGAGTTTGTTGGACAATGGGAAGTGTCAGAAACGTAACAGAGCTGTTGCGTTTGGTTGTAACCATAT GGAAGAGTCGTGTTCAGAGTTTGATAGGTCTAGGAAGAGGTTTAGAGAGAGTGATGATGATGATAAAGGAGTAGGACTGTCTAGTGTTGTTATTAGGGAG GAGACTGTTGTATGTGCTGTGGAAGAAGGTTTGTCGGATTTTTCCTTGGAGAAGCGAGACGATTTACCGGGAATGTTGAAGTGGCTGGCTTTGGTTGCAA CATCTCCTCATGATCCTGCCATTGGAGTTATACCGCATAGCTCAAAATGGAAGCAGGATAACGGTAACAAGTGTTGGCTCCAAGTGGCTAGAGCAAAAAA CTCTTTGCTGGTTCAAAGAGACAATGCTGAACTCAGATATAGATATCATCCATTTCGGGGTCACCAAAATATACATCATCCGTCTATGTATGAAGATGAT CGAAAGTCGATAGGGAGGTTAAGATACAGCATAAGACCTCCAAATTTATCCAAACACTGTTCGAGCTCGTGTTGCAATGGAAGCAGTTTGGTCTCTCTAT CTAAATCAAGATCTACCAAATGCAGAAAACTGACGATCATTGCTTCTGAGAGAGCAGGCCTTACTGCAGGAACATCTAGAGCCAGAAAGCGAAACAAAGC AGAGATCCCGAGAAGGTGTATTAAAGTAGGACATCAACACCAAGCCCAAGTGGATGAATGGACAGAGTCCGGTGTAGATAGCGATAGTAAATGGTTAGGA ACCCGTATATGGCCGCCAGAAAATAGTGAAGCTTTAGATCAGACGCTTGGAAATGATTTAGTTGGAAAAGGAAGGCCAGATTCTTGCAGCTGTGAGCTCT CTGGTTTTGTGGAATGCACTAGGTTGCACATTGCAGAGAAGAGAATGGAACTGAAACGTGAGCTCGGCGATGACTTTTTCCATTGGAGATTCAATCAAAT GGGAGAAGAAGTGTGTCTAAGATGGACAGAAGAAGAAGAGAAGAGGTTTAAAGACATGATCATCGCGGATCCTCAATCTTTCTGGACAAATGCGGCAAAA AATTTCCCAAAGAAGAAGAGAGAGGAGCTTGTGAGTTACTACTTCAACGTCTTCTTGATTAACAGAAGACGGTATCAGAACCGTGTGACTCCAAAAAGTA TAGACAGTGATGACGAAGGAGCGTTTGGTAGTGTTGGTGGCAGTTTTGGTCGTGATGCTGTTACTTCGTCTGGTTCAGATGTTATGATCTGTGCTCAGAA TCGTCAGTGTGAGGACTAC >IE_07E02_translated MGSFNDTSCSYVDVEIKYVDECEERLRRLFDQALLVFLEEEGSIKPLPAVIGDGKNVDLFKLFVLVREREGFDTVSRKRLWEVVAEKLGFDCSLVPSLIL IYLKYLNRMEKWAVEESRIVNWDNKDSEKKGCYSGMLHELGNGFKSLLDNGKCQKRNRAVAFGCNHMEESCSEFDRSRKRFRESDDDDKGVGLSSVVIRE ETVVCAVEEGLSDFSLEKRDDLPGMLKWLALVATSPHDPAIGVIPHSSKWKQDNGNKCWLQVARAKNSLLVQRDNAELRYRYHPFRGHQNIHHPSMYEDD RKSIGRLRYSIRPPNLSKHCSSSCCNGSSLVSLSKSRSTKCRKLTIIASERAGLTAGTSRARKRNKAEIPRRCIKVGHQHQAQVDEWTESGVDSDSKWLG TRIWPPENSEALDQTLGNDLVGKGRPDSCSCELSGFVECTRLHIAEKRMELKRELGDDFFHWRFNQMGEEVCLRWTEEEEKRFKDMIIADPQSFWTNAAK NFPKKKREELVSYYFNVFLINRRRYQNRVTPKSIDSDDEGAFGSVGGSFGRDAVTSSGSDVMICAQNRQCEDY
Alignment	◆CDS（TAIRver.9）_without_stop_codonとのalignment◆ 1717/1719 (99.9%)（アミノ酸配列で572/573 (99.8%)）一致しました。 10 20 30 40 50 60 70 80 90 100 AT4G11 ATGGGAAGTTTTAATGATACTTCATGTTCATATGTTGATGTTGAGATTAAGTATGTTGATGAATGTGAGGAGAGGTTAAGGAGATTGTTTGATCAAGCTT :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_07E ATGGGAAGTTTTAATGATACTTCATGTTCATATGTTGATGTTGAGATTAAGTATGTTGATGAATGTGAGGAGAGGTTAAGGAGATTGTTTGATCAAGCTT 10 20 30 40 50 60 70 80 90 100 110 120 130 140 150 160 170 180 190 200 AT4G11 TGTTGGTTTTTCTTGAGGAAGAGGGTAGTATAAAGCCTCTCCCTGCAGTTATAGGTGATGGCAAAAATGTTGATTTGTTTAAGCTGTTTGTATTGGTGAG :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_07E TGTTGGTTTTTCTTGAGGAAGAGGGTAGTATAAAGCCTCTCCCTGCAGTTATAGGTGATGGCAAAAATGTTGATTTGTTTAAGCTGTTTGTATTGGTGAG 110 120 130 140 150 160 170 180 190 200 210 220 230 240 250 260 270 280 290 300 AT4G11 GGAGAGGGAAGGGTTTGATACTGTGTCTAGGAAAAGGTTGTGGGAAGTAGTGGCTGAGAAGTTAGGCTTTGATTGTTCACTGGTTCCTTCTTTGATATTG :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_07E GGAGAGGGAAGGGTTTGATACTGTGTCTAGGAAAAGGTTGTGGGAAGTAGTGGCTGAGAAGTTAGGCTTTGATTGTTCACTGGTTCCTTCTTTGATATTG 210 220 230 240 250 260 270 280 290 300 310 320 330 340 350 360 370 380 390 400 AT4G11 ATTTACTTGAAGTATTTGAACCGGATGGAGAAATGGGCGGTGGAGGAATCTAGGATTGTGAATTGGGATAATAAGGATAGTGAAAAGAAAGGGTGTTATA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_07E ATTTACTTGAAGTATTTGAACCGGATGGAGAAATGGGCGGTGGAGGAATCTAGGATTGTGAATTGGGATAATAAGGATAGTGAAAAGAAAGGGTGTTATA 310 320 330 340 350 360 370 380 390 400 410 420 430 440 450 460 470 480 490 500 AT4G11 GTGGTATGTTGCATGAGTTAGGGAATGGTTTTAAGAGTTTGTTGGACAATGGGAAGTGTCAGAAACGTAACAGAGCTGTTGCGTTTGGTTGTAACCATAT :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_07E GTGGTATGTTGCATGAGTTAGGGAATGGTTTTAAGAGTTTGTTGGACAATGGGAAGTGTCAGAAACGTAACAGAGCTGTTGCGTTTGGTTGTAACCATAT 410 420 430 440 450 460 470 480 490 500 510 520 530 540 550 560 570 580 590 600 AT4G11 GGAAGAGTCGTGTTCAGAGTTTGATAGGTCTAGGAAGAGGTTTAGAGAGAGTGATGATGATGATAAAGGAGTAGGACTGTCTAGTGTTGTTATTAGGGAG :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_07E GGAAGAGTCGTGTTCAGAGTTTGATAGGTCTAGGAAGAGGTTTAGAGAGAGTGATGATGATGATAAAGGAGTAGGACTGTCTAGTGTTGTTATTAGGGAG 510 520 530 540 550 560 570 580 590 600 610 620 630 640 650 660 670 680 690 700 AT4G11 GAGACTGTTGTATGTGCTGTGGAAGAAGGTTTGTCGGATTTTTCCTTGGAGAAGCGAGACGATTTACCGGGAATGTTGAAGTGGCTGGCTTTGGTTGCAA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_07E GAGACTGTTGTATGTGCTGTGGAAGAAGGTTTGTCGGATTTTTCCTTGGAGAAGCGAGACGATTTACCGGGAATGTTGAAGTGGCTGGCTTTGGTTGCAA 610 620 630 640 650 660 670 680 690 700 710 720 730 740 750 760 770 780 790 800 AT4G11 CATCTCCTCATGATCCTGCCATTGGAGTTATACCGCATAGTTCAAAATGGAAGCAGTATAACGGTAACAAGTGTTGGCTCCAAGTGGCTAGAGCAAAAAA :::::::::::::::::::::::::::::::::::::::: ::::::::::::::: ::::::::::::::::::::::::::::::::::::::::::: IE_07E CATCTCCTCATGATCCTGCCATTGGAGTTATACCGCATAGCTCAAAATGGAAGCAGGATAACGGTAACAAGTGTTGGCTCCAAGTGGCTAGAGCAAAAAA 710 720 730 740 750 760 770 780 790 800 810 820 830 840 850 860 870 880 890 900 AT4G11 CTCTTTGCTGGTTCAAAGAGACAATGCTGAACTCAGATATAGATATCATCCATTTCGGGGTCACCAAAATATACATCATCCGTCTATGTATGAAGATGAT :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_07E CTCTTTGCTGGTTCAAAGAGACAATGCTGAACTCAGATATAGATATCATCCATTTCGGGGTCACCAAAATATACATCATCCGTCTATGTATGAAGATGAT 810 820 830 840 850 860 870 880 890 900 910 920 930 940 950 960 970 980 990 1000 AT4G11 CGAAAGTCGATAGGGAGGTTAAGATACAGCATAAGACCTCCAAATTTATCCAAACACTGTTCGAGCTCGTGTTGCAATGGAAGCAGTTTGGTCTCTCTAT :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_07E CGAAAGTCGATAGGGAGGTTAAGATACAGCATAAGACCTCCAAATTTATCCAAACACTGTTCGAGCTCGTGTTGCAATGGAAGCAGTTTGGTCTCTCTAT 910 920 930 940 950 960 970 980 990 1000 1010 1020 1030 1040 1050 1060 1070 1080 1090 1100 AT4G11 CTAAATCAAGATCTACCAAATGCAGAAAACTGACGATCATTGCTTCTGAGAGAGCAGGCCTTACTGCAGGAACATCTAGAGCCAGAAAGCGAAACAAAGC :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_07E CTAAATCAAGATCTACCAAATGCAGAAAACTGACGATCATTGCTTCTGAGAGAGCAGGCCTTACTGCAGGAACATCTAGAGCCAGAAAGCGAAACAAAGC 1010 1020 1030 1040 1050 1060 1070 1080 1090 1100 1110 1120 1130 1140 1150 1160 1170 1180 1190 1200 AT4G11 AGAGATCCCGAGAAGGTGTATTAAAGTAGGACATCAACACCAAGCCCAAGTGGATGAATGGACAGAGTCCGGTGTAGATAGCGATAGTAAATGGTTAGGA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_07E AGAGATCCCGAGAAGGTGTATTAAAGTAGGACATCAACACCAAGCCCAAGTGGATGAATGGACAGAGTCCGGTGTAGATAGCGATAGTAAATGGTTAGGA 1110 1120 1130 1140 1150 1160 1170 1180 1190 1200 1210 1220 1230 1240 1250 1260 1270 1280 1290 1300 AT4G11 ACCCGTATATGGCCGCCAGAAAATAGTGAAGCTTTAGATCAGACGCTTGGAAATGATTTAGTTGGAAAAGGAAGGCCAGATTCTTGCAGCTGTGAGCTCT :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_07E ACCCGTATATGGCCGCCAGAAAATAGTGAAGCTTTAGATCAGACGCTTGGAAATGATTTAGTTGGAAAAGGAAGGCCAGATTCTTGCAGCTGTGAGCTCT 1210 1220 1230 1240 1250 1260 1270 1280 1290 1300 1310 1320 1330 1340 1350 1360 1370 1380 1390 1400 AT4G11 CTGGTTTTGTGGAATGCACTAGGTTGCACATTGCAGAGAAGAGAATGGAACTGAAACGTGAGCTCGGCGATGACTTTTTCCATTGGAGATTCAATCAAAT :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_07E CTGGTTTTGTGGAATGCACTAGGTTGCACATTGCAGAGAAGAGAATGGAACTGAAACGTGAGCTCGGCGATGACTTTTTCCATTGGAGATTCAATCAAAT 1310 1320 1330 1340 1350 1360 1370 1380 1390 1400 1410 1420 1430 1440 1450 1460 1470 1480 1490 1500 AT4G11 GGGAGAAGAAGTGTGTCTAAGATGGACAGAAGAAGAAGAGAAGAGGTTTAAAGACATGATCATCGCGGATCCTCAATCTTTCTGGACAAATGCGGCAAAA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_07E GGGAGAAGAAGTGTGTCTAAGATGGACAGAAGAAGAAGAGAAGAGGTTTAAAGACATGATCATCGCGGATCCTCAATCTTTCTGGACAAATGCGGCAAAA 1410 1420 1430 1440 1450 1460 1470 1480 1490 1500 1510 1520 1530 1540 1550 1560 1570 1580 1590 1600 AT4G11 AATTTCCCAAAGAAGAAGAGAGAGGAGCTTGTGAGTTACTACTTCAACGTCTTCTTGATTAACAGAAGACGGTATCAGAACCGTGTGACTCCAAAAAGTA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_07E AATTTCCCAAAGAAGAAGAGAGAGGAGCTTGTGAGTTACTACTTCAACGTCTTCTTGATTAACAGAAGACGGTATCAGAACCGTGTGACTCCAAAAAGTA 1510 1520 1530 1540 1550 1560 1570 1580 1590 1600 1610 1620 1630 1640 1650 1660 1670 1680 1690 1700 AT4G11 TAGACAGTGATGACGAAGGAGCGTTTGGTAGTGTTGGTGGCAGTTTTGGTCGTGATGCTGTTACTTCGTCTGGTTCAGATGTTATGATCTGTGCTCAGAA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_07E TAGACAGTGATGACGAAGGAGCGTTTGGTAGTGTTGGTGGCAGTTTTGGTCGTGATGCTGTTACTTCGTCTGGTTCAGATGTTATGATCTGTGCTCAGAA 1610 1620 1630 1640 1650 1660 1670 1680 1690 1700 1710 AT4G11 TCGTCAGTGTGAGGACTAC ::::::::::::::::::: IE_07E TCGTCAGTGTGAGGACTAC 1710 10 20 30 40 50 60 70 80 90 100 AT4G11 MGSFNDTSCSYVDVEIKYVDECEERLRRLFDQALLVFLEEEGSIKPLPAVIGDGKNVDLFKLFVLVREREGFDTVSRKRLWEVVAEKLGFDCSLVPSLIL :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_07E MGSFNDTSCSYVDVEIKYVDECEERLRRLFDQALLVFLEEEGSIKPLPAVIGDGKNVDLFKLFVLVREREGFDTVSRKRLWEVVAEKLGFDCSLVPSLIL 10 20 30 40 50 60 70 80 90 100 110 120 130 140 150 160 170 180 190 200 AT4G11 IYLKYLNRMEKWAVEESRIVNWDNKDSEKKGCYSGMLHELGNGFKSLLDNGKCQKRNRAVAFGCNHMEESCSEFDRSRKRFRESDDDDKGVGLSSVVIRE :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_07E IYLKYLNRMEKWAVEESRIVNWDNKDSEKKGCYSGMLHELGNGFKSLLDNGKCQKRNRAVAFGCNHMEESCSEFDRSRKRFRESDDDDKGVGLSSVVIRE 110 120 130 140 150 160 170 180 190 200 210 220 230 240 250 260 270 280 290 300 AT4G11 ETVVCAVEEGLSDFSLEKRDDLPGMLKWLALVATSPHDPAIGVIPHSSKWKQYNGNKCWLQVARAKNSLLVQRDNAELRYRYHPFRGHQNIHHPSMYEDD :::::::::::::::::::::::::::::::::::::::::::::::::::: ::::::::::::::::::::::::::::::::::::::::::::::: IE_07E ETVVCAVEEGLSDFSLEKRDDLPGMLKWLALVATSPHDPAIGVIPHSSKWKQDNGNKCWLQVARAKNSLLVQRDNAELRYRYHPFRGHQNIHHPSMYEDD 210 220 230 240 250 260 270 280 290 300 310 320 330 340 350 360 370 380 390 400 AT4G11 RKSIGRLRYSIRPPNLSKHCSSSCCNGSSLVSLSKSRSTKCRKLTIIASERAGLTAGTSRARKRNKAEIPRRCIKVGHQHQAQVDEWTESGVDSDSKWLG :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_07E RKSIGRLRYSIRPPNLSKHCSSSCCNGSSLVSLSKSRSTKCRKLTIIASERAGLTAGTSRARKRNKAEIPRRCIKVGHQHQAQVDEWTESGVDSDSKWLG 310 320 330 340 350 360 370 380 390 400 410 420 430 440 450 460 470 480 490 500 AT4G11 TRIWPPENSEALDQTLGNDLVGKGRPDSCSCELSGFVECTRLHIAEKRMELKRELGDDFFHWRFNQMGEEVCLRWTEEEEKRFKDMIIADPQSFWTNAAK :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_07E TRIWPPENSEALDQTLGNDLVGKGRPDSCSCELSGFVECTRLHIAEKRMELKRELGDDFFHWRFNQMGEEVCLRWTEEEEKRFKDMIIADPQSFWTNAAK 410 420 430 440 450 460 470 480 490 500 510 520 530 540 550 560 570 AT4G11 NFPKKKREELVSYYFNVFLINRRRYQNRVTPKSIDSDDEGAFGSVGGSFGRDAVTSSGSDVMICAQNRQCEDY ::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_07E NFPKKKREELVSYYFNVFLINRRRYQNRVTPKSIDSDDEGAFGSVGGSFGRDAVTSSGSDVMICAQNRQCEDY 510 520 530 540 550 560 570

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âââIE_07E02 detailâââ