| Code name | IE_07F02 | |||
|---|---|---|---|---|
| Locus | AT5G64220 | |||
| Template | IT_07F02 (ABRC_215) | |||
| Donor Vector | pDONR207 | |||
| Forward primer | IPF_07F02 (ggggacaagtttgtacaaaaaagcaggcttcATGGCGGATCGCGGATCTTTCGGATTT) | |||
| Reverse primer | IPR_07F02 (ggggaccactttgtacaagaaagctgggttTTCAAATGCAAGAGACATGAAAGTGTCGTC) | |||
| Alignment with template seq. | 1st | 配列解析が行えなかったサンプルです。 | 2nd | アミノ酸配列100%一致 |
| Alignment with TAIR7CDS | - | アミノ酸配列100%一致 | ||
| comment | 1st:(Sequence解析まで行ったが、読み直す必要がある) | |||
| sequence |
>IE_07F02_3150bp ATGGCGGATCGCGGATCTTTCGGATTTGCTCCTCGATTAGATATCAAGCAATTGCTGTCAGAAGCACAACACAGGTGGCTGAGACCTGCTGAGATTTGTG AAATTCTTCGGAATCATCAGAAGTTTCATATTGCGTCAGAGCCTCCCAACCGACCACCCAGTGGTTCACTCTTTCTCTTCGATAGGAAGGTGCTCAGATA TTTCCGAAAAGACGGGCACAATTGGAGGAAGAAGAAAGATGGGAAAACAGTGAAAGAGGCTCACGAAAAGCTGAAGGTAGGAAGCATTGATGTGTTACAT TGTTACTATGCACATGGAGAAGATAATGAGAACTTCCAGAGGCGGTGCTACTGGATGCTTGAACAGGATCTGATGCACATTGTTTTCGTTCACTACTTGG AGGTTAAGGGTAACCGAATGAGTACCAGTGGAACTAAAGAAAACCATTCAAATTCTCTGAGTGGCACGGGTTCTGTAAATGTTGATTCCACAGCAACCCG ATCCAGCATATTGTCACCATTATGTGAAGATGCTGATTCTGGGGATAGTCGTCAAGCAAGTTCCAGTTTGCAACAAAATCCTGAACCTCAAACTGTTGTT CCTCAGATTATGCATCATCAGAATGCTAGCACAATCAATTCTTACAATACAACCTCTGTTTTGGGAAATCGAGATGGTTGGACGTCAGCTCATGGGAACA GGGTCAAAGGAAGTAACTCCCAAAGATCAGGTGATGTCCCAGCATGGGATGCTTCCTTTGAGAATTCATTGGCAAGATACCAGAATCTACCTTATAATGC TCCGTTGACTCAGACACAGCCTTCTACTTTTGGTTTGATCCCCATGGAAGGGAAAACAGAGAAAGGTTCCCTGTTAACGTCAGAGCATCTTAGAAATCCT CTTCAAAGTCAAGTAAATTGGCAGACTCCTGTTCAGGAAAGCGTGCCATTGCAAAAATGGCCCATGGATTCGCATTCTGGAATGACTGATGCTACTGATC TTGCCTTATTTGGGCAAGGAGCACATGAAAATTTTGGGACATTTTCCAGTCTTCTTGGTAGTCAAGATCAACAGTCTAGTAGTTTCCAAGCTCCTTTCAC AAATAATGAAGCAGCATATATACCTAAATTAGGCCCCGAGGATCTTATATATGAAGCAAGTGCAAATCAAACACTACCTCTTAGAAAAGCATTGTTAAAA AAAGAGGATAGTTTGAAAAAGGTTGACAGTTTCTCTCGGTGGGTTAGTAAAGAACTTGGGGAGATGGAAGACTTGCAGATGCAATCTTCATCTGGAGGTA TTGCATGGACTTCTGTTGAATGTGAAAACGCAGCAGCTGGGTCTTCCTTAAGTCCTTCTCTGTCCGAGGATCAGCGCTTCACTATGATCGACTTTTGGCC CAAATGGACTCAGACAGACTCAGAAGTTGAGGTAATGGTTATCGGGACATTTCTGTTGAGTCCTCAGGAAGTAACTAGCTATAGCTGGTCATGCATGTTT GGAGAAGTGGAGGTTCCGGCTGATATTTTAGTAGATGGTGTTCTTTGTTGTCATGCTCCGCCACATGAAGTCGGTCGAGTCCCGTTCTATATAACATGTT CTGACAGATTTTCTTGCAGTGAAGTACGAGAATTCGATTTCCTTCCTGGGTCTACCAGAAAGTTAAATGCTACTGACATTTATGGTGCCAATACAATTGA AACATCACTCCATTTGCGGTTTGAAAACTTGCTGGCTCTCAGATGTTCTGTTCAGGAACACCATATATTTGAAAATGTTGGGGAGAAACGAAGAAAAATC AGCAAAATCATGTTGCTAAAGGATGAAAAAGAGCCTCCTCTCCCCGGAACAATTGAGAAAGATCTAACAGAGCTGGAAGCAAAAGAGCGATTGATTAGGG AAGAGTTTGAAGATAAATTATATCTGTGGCTCATCCATAAGGTGACTGAAGAGGGCAAGGGTCCAAATATATTAGATGAAGATGGACAGGGTGTTCTACA CCTAGCCGCAGCGCTGGGGTATGATTGGGCGATAAAGCCAATCTTAGCAGCAGGAGTTAGCATCAACTTCCGCGATGCAAATGGGTGGTCTGCTCTTCAC TGGGCTGCATTCAGTGGCAGGGAGGATACTGTCGCTGTACTTGTCTCACTGGGTGCTGATGCTGGAGCATTGGCGGACCCATCTCCAGAACATCCTTTGG GGAAAACAGCTGCTGACTTGGCTTATGGAAACGGACACAGGGGAATATCGGGTTTCCTTGCAGAGTCATCCCTCACTAGCTACCTTGAAAAACTAACAGT GGACGCTAAGGAAAACAGCTCCGCTGACTCCTCTGGAGCAAAAGCTGTTCTGACAGTAGCTGAGCGAACAGCTACTCCTATGAGTTATGGTGATGTACCA GAAACACTTTCGATGAAGGATTCACTTACTGCTGTCTTAAATGCTACACAAGCAGCTGATCGTCTTCATCAAGTGTTCAGAATGCAGTCGTTCCAGCGGA AACAGCTCTCTGAACTTGGTGGCGACAACAAATTCGATATATCCGATGAGTTAGCTGTGTCTTTTGCGGCTGCAAAGACTAAGAAGTCAGGACACAGTAG TGGTGCTGTTCATGCTGCTGCGGTTCAAATCCAGAAAAAATACCGTGGTTGGAAGAAGAGAAAGGAGTTCCTTCTGATCCGACAAAGAATCGTCAAGATT CAGGCTCATGTGAGAGGACATCAAGTCCGAAAACAATACCGAGCCATTATTTGGTCCGTAGGATTGCTTGAGAAAATCATCTTGCGATGGAGGCGTAAAG GTAGTGGCTTACGCGGTTTCAAACGTGATACAATCAGCAAGCCAACAGAACCTGTATGTCCTGCCCCACAAGAAGATGATTATGATTTTCTCAAGGAAGG AAGGAAACAAACAGAGGAAAGGCTTCAAAAGGCTCTAACCCGAGTGAAGTCTATGGCTCAATATCCAGAGGCACGGGCTCAATACCGTAGACTATTGACT GTCGTTGAAGGCTTCCGTGAGAACGAGGCTTCTTCTAGCTCGGCATTGAAAAACAACACAGAGGAAGCTGCAAATTACAACGAAGAAGACGATTTAATTG ACATTGATTCTCTTTTGGATGACGACACTTTCATGTCTCTTGCATTTGAA >IE_07F02_translated MADRGSFGFAPRLDIKQLLSEAQHRWLRPAEICEILRNHQKFHIASEPPNRPPSGSLFLFDRKVLRYFRKDGHNWRKKKDGKTVKEAHEKLKVGSIDVLH CYYAHGEDNENFQRRCYWMLEQDLMHIVFVHYLEVKGNRMSTSGTKENHSNSLSGTGSVNVDSTATRSSILSPLCEDADSGDSRQASSSLQQNPEPQTVV PQIMHHQNASTINSYNTTSVLGNRDGWTSAHGNRVKGSNSQRSGDVPAWDASFENSLARYQNLPYNAPLTQTQPSTFGLIPMEGKTEKGSLLTSEHLRNP LQSQVNWQTPVQESVPLQKWPMDSHSGMTDATDLALFGQGAHENFGTFSSLLGSQDQQSSSFQAPFTNNEAAYIPKLGPEDLIYEASANQTLPLRKALLK KEDSLKKVDSFSRWVSKELGEMEDLQMQSSSGGIAWTSVECENAAAGSSLSPSLSEDQRFTMIDFWPKWTQTDSEVEVMVIGTFLLSPQEVTSYSWSCMF GEVEVPADILVDGVLCCHAPPHEVGRVPFYITCSDRFSCSEVREFDFLPGSTRKLNATDIYGANTIETSLHLRFENLLALRCSVQEHHIFENVGEKRRKI SKIMLLKDEKEPPLPGTIEKDLTELEAKERLIREEFEDKLYLWLIHKVTEEGKGPNILDEDGQGVLHLAAALGYDWAIKPILAAGVSINFRDANGWSALH WAAFSGREDTVAVLVSLGADAGALADPSPEHPLGKTAADLAYGNGHRGISGFLAESSLTSYLEKLTVDAKENSSADSSGAKAVLTVAERTATPMSYGDVP ETLSMKDSLTAVLNATQAADRLHQVFRMQSFQRKQLSELGGDNKFDISDELAVSFAAAKTKKSGHSSGAVHAAAVQIQKKYRGWKKRKEFLLIRQRIVKI QAHVRGHQVRKQYRAIIWSVGLLEKIILRWRRKGSGLRGFKRDTISKPTEPVCPAPQEDDYDFLKEGRKQTEERLQKALTRVKSMAQYPEARAQYRRLLT VVEGFRENEASSSSALKNNTEEAANYNEEDDLIDIDSLLDDDTFMSLAFE |
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| Alignment |
◆CDS（TAIRver.9）_without_stop_codonとのalignment◆ 100%一致しました。 |
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