âââIE_07F04 detailâââ

Code name	IE_07F04
Locus	At5g21120
Template	IT_07F04 (ABRC_221)
Donor Vector	pDONR207
Forward primer	IPF_07F04 (ggggacaagtttgtacaaaaaagcaggcttcATGGATATGTATAACAACAATATAGGG)
Reverse primer	IPR_07F04 (ggggaccactttgtacaagaaagctgggttCTGAATCCAAGATGTGGGCAACTCTTGCCC)
Alignment with template seq.	1st	éåè§£æãè¡ããªãã£ããµã³ãã«ã§ãã	2nd	ã¢ããé¸éå100%ä¸è´
Alignment with TAIR7CDS	1st	-	2nd	ã¢ããé¸éå100%ä¸è´
comment	1st:(Sequenceè§£æã¾ã§è¡ã£ããããã¬ã¼ã ã¯ãããªããå¡©åºç½®æããã), 2nd:70%, nt.1182T→C
sequence	>IE_07F04_1554bp ATGGATATGTATAACAACAATATAGGGATGTTCCGGAGTTTAGTTTGTAGCTCGGCGCCTCCATTTACAGAGGGACATATGTGTTCTGATTCGCATACGG CTTTGTGCGATGATCTGAGTAGTGATGAGGAAATGGAAATAGAGGAGCTTGAGAAGAAGATCTGGAGAGACAAGCAGCGTTTAAAGCGGCTCAAGGAAAT GGCGAAGAACGGTCTAGGAACAAGATTGTTGTTGAAGCAGCAACATGATGATTTTCCAGAGCACTCTAGTAAGAGAACCATGTACAAGGCACAAGATGGG ATCTTGAAGTACATGTCGAAGACAATGGAGCGATATAAAGCTCAAGGTTTTGTTTATGGGATTGTGTTAGAGAATGGGAAAACGGTAGCGGGATCTTCTG ATAATCTCCGTGAATGGTGGAAAGACAAAGTGAGGTTTGATAGGAACGGCCCAGCTGCTATAATCAAGCACCAAAGGGATATCAATCTTTCTGATGGAAG TGATTCAGGGTCTGAGGTTGGGGATTCTACCGCACAGAAGTTGCTTGAGCTTCAAGATACTACTCTTGGAGCTCTGTTATCGGCTCTGTTTCCTCACTGC AACCCTCCTCAGAGGCGGTTTCCGTTGGAGAAAGGCGTGACACCGCCATGGTGGCCAACGGGGAAAGAAGATTGGTGGGATCAACTGTCTTTACCCGTTG ATTTTCGAGGTGTTCCGCCACCTTACAAGAAGCCTCATGATCTCAAGAAGCTGTGGAAAATTGGTGTTTTGATTGGTGTAATCAGACATATGGCTTCTGA CATTAGCAACATACCCAATCTCGTGAGACGGTCTAGAAGTTTGCAGGAGAAAATGACGTCAAGAGAAGGCGCTTTATGGCTCGCTGCTCTTTACCGAGAA AAGGCTATTGTTGATCAAATAGCCATGTCTAGAGAAAACAACAACACTTCTAACTTTCTTGTTCCTGCAACCGGTGGAGACCCAGATGTTTTGTTTCCTG AATCTACAGACTATGATGTTGAACTGATTGGTGGCACTCATCGGACCAATCAGCAGTATCCTGAATTTGAAAACAACTACAACTGTGTTTACAAGAGAAA GTTTGAAGAAGATTTTGGGATGCCAATGCATCCAACACTCCTAACATGTGAGAACAGTCTCTGTCCTTATAGCCAACCACACATGGGATTTCTTGACAGG AACTTAAGAGAGAATCACCAAATGACTTGTCCTTATAAAGTCACTTCCTTCTACCAACCAACTAAACCCTATGGTATGACGGGTTTAATGGTTCCTTGTC CGGATTATAACGGGATGCAGCAGCAGGTTCAGAGCTTTCAAGACCAGTTTAATCATCCCAACGATCTCTACAGACCAAAAGCTCCACAAAGAGGCAACGA TGACTTGGTTGAGGATTTGAATCCTTCTCCTTCGACGCTGAATCAGAATCTTGGTTTAGTCTTACCTACTGACTTCAATGGAGGTGAGGAAACAGTAGGA ACAGAGAACAATCTGCATAATCAAGGGCAAGAGTTGCCCACATCTTGGATTCAG >IE_07F04_translated MDMYNNNIGMFRSLVCSSAPPFTEGHMCSDSHTALCDDLSSDEEMEIEELEKKIWRDKQRLKRLKEMAKNGLGTRLLLKQQHDDFPEHSSKRTMYKAQDG ILKYMSKTMERYKAQGFVYGIVLENGKTVAGSSDNLREWWKDKVRFDRNGPAAIIKHQRDINLSDGSDSGSEVGDSTAQKLLELQDTTLGALLSALFPHC NPPQRRFPLEKGVTPPWWPTGKEDWWDQLSLPVDFRGVPPPYKKPHDLKKLWKIGVLIGVIRHMASDISNIPNLVRRSRSLQEKMTSREGALWLAALYRE KAIVDQIAMSRENNNTSNFLVPATGGDPDVLFPESTDYDVELIGGTHRTNQQYPEFENNYNCVYKRKFEEDFGMPMHPTLLTCENSLCPYSQPHMGFLDR NLRENHQMTCPYKVTSFYQPTKPYGMTGLMVPCPDYNGMQQQVQSFQDQFNHPNDLYRPKAPQRGNDDLVEDLNPSPSTLNQNLGLVLPTDFNGGEETVG TENNLHNQGQELPTSWIQ
Alignment	◆CDS（TAIRver.9）_without_stop_codonとのalignment◆ 1553/1554 (99.9%)（アミノ酸配列で100%）一致しました。 10 20 30 40 50 60 70 80 90 100 AT5G21 ATGGATATGTATAACAACAATATAGGGATGTTCCGGAGTTTAGTTTGTAGCTCGGCGCCTCCATTTACAGAGGGACATATGTGTTCTGATTCGCATACGG :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_07F ATGGATATGTATAACAACAATATAGGGATGTTCCGGAGTTTAGTTTGTAGCTCGGCGCCTCCATTTACAGAGGGACATATGTGTTCTGATTCGCATACGG 10 20 30 40 50 60 70 80 90 100 110 120 130 140 150 160 170 180 190 200 AT5G21 CTTTGTGCGATGATCTGAGTAGTGATGAGGAAATGGAAATAGAGGAGCTTGAGAAGAAGATCTGGAGAGACAAGCAGCGTTTAAAGCGGCTCAAGGAAAT :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_07F CTTTGTGCGATGATCTGAGTAGTGATGAGGAAATGGAAATAGAGGAGCTTGAGAAGAAGATCTGGAGAGACAAGCAGCGTTTAAAGCGGCTCAAGGAAAT 110 120 130 140 150 160 170 180 190 200 210 220 230 240 250 260 270 280 290 300 AT5G21 GGCGAAGAACGGTCTAGGAACAAGATTGTTGTTGAAGCAGCAACATGATGATTTTCCAGAGCACTCTAGTAAGAGAACCATGTACAAGGCACAAGATGGG :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_07F GGCGAAGAACGGTCTAGGAACAAGATTGTTGTTGAAGCAGCAACATGATGATTTTCCAGAGCACTCTAGTAAGAGAACCATGTACAAGGCACAAGATGGG 210 220 230 240 250 260 270 280 290 300 310 320 330 340 350 360 370 380 390 400 AT5G21 ATCTTGAAGTACATGTCGAAGACAATGGAGCGATATAAAGCTCAAGGTTTTGTTTATGGGATTGTGTTAGAGAATGGGAAAACGGTAGCGGGATCTTCTG :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_07F ATCTTGAAGTACATGTCGAAGACAATGGAGCGATATAAAGCTCAAGGTTTTGTTTATGGGATTGTGTTAGAGAATGGGAAAACGGTAGCGGGATCTTCTG 310 320 330 340 350 360 370 380 390 400 410 420 430 440 450 460 470 480 490 500 AT5G21 ATAATCTCCGTGAATGGTGGAAAGACAAAGTGAGGTTTGATAGGAACGGCCCAGCTGCTATAATCAAGCACCAAAGGGATATCAATCTTTCTGATGGAAG :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_07F ATAATCTCCGTGAATGGTGGAAAGACAAAGTGAGGTTTGATAGGAACGGCCCAGCTGCTATAATCAAGCACCAAAGGGATATCAATCTTTCTGATGGAAG 410 420 430 440 450 460 470 480 490 500 510 520 530 540 550 560 570 580 590 600 AT5G21 TGATTCAGGGTCTGAGGTTGGGGATTCTACCGCACAGAAGTTGCTTGAGCTTCAAGATACTACTCTTGGAGCTCTGTTATCGGCTCTGTTTCCTCACTGC :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_07F TGATTCAGGGTCTGAGGTTGGGGATTCTACCGCACAGAAGTTGCTTGAGCTTCAAGATACTACTCTTGGAGCTCTGTTATCGGCTCTGTTTCCTCACTGC 510 520 530 540 550 560 570 580 590 600 610 620 630 640 650 660 670 680 690 700 AT5G21 AACCCTCCTCAGAGGCGGTTTCCGTTGGAGAAAGGCGTGACACCGCCATGGTGGCCAACGGGGAAAGAAGATTGGTGGGATCAACTGTCTTTACCCGTTG :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_07F AACCCTCCTCAGAGGCGGTTTCCGTTGGAGAAAGGCGTGACACCGCCATGGTGGCCAACGGGGAAAGAAGATTGGTGGGATCAACTGTCTTTACCCGTTG 610 620 630 640 650 660 670 680 690 700 710 720 730 740 750 760 770 780 790 800 AT5G21 ATTTTCGAGGTGTTCCGCCACCTTACAAGAAGCCTCATGATCTCAAGAAGCTGTGGAAAATTGGTGTTTTGATTGGTGTAATCAGACATATGGCTTCTGA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_07F ATTTTCGAGGTGTTCCGCCACCTTACAAGAAGCCTCATGATCTCAAGAAGCTGTGGAAAATTGGTGTTTTGATTGGTGTAATCAGACATATGGCTTCTGA 710 720 730 740 750 760 770 780 790 800 810 820 830 840 850 860 870 880 890 900 AT5G21 CATTAGCAACATACCCAATCTCGTGAGACGGTCTAGAAGTTTGCAGGAGAAAATGACGTCAAGAGAAGGCGCTTTATGGCTCGCTGCTCTTTACCGAGAA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_07F CATTAGCAACATACCCAATCTCGTGAGACGGTCTAGAAGTTTGCAGGAGAAAATGACGTCAAGAGAAGGCGCTTTATGGCTCGCTGCTCTTTACCGAGAA 810 820 830 840 850 860 870 880 890 900 910 920 930 940 950 960 970 980 990 1000 AT5G21 AAGGCTATTGTTGATCAAATAGCCATGTCTAGAGAAAACAACAACACTTCTAACTTTCTTGTTCCTGCAACCGGTGGAGACCCAGATGTTTTGTTTCCTG :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_07F AAGGCTATTGTTGATCAAATAGCCATGTCTAGAGAAAACAACAACACTTCTAACTTTCTTGTTCCTGCAACCGGTGGAGACCCAGATGTTTTGTTTCCTG 910 920 930 940 950 960 970 980 990 1000 1010 1020 1030 1040 1050 1060 1070 1080 1090 1100 AT5G21 AATCTACAGACTATGATGTTGAACTGATTGGTGGCACTCATCGGACCAATCAGCAGTATCCTGAATTTGAAAACAACTACAACTGTGTTTACAAGAGAAA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_07F AATCTACAGACTATGATGTTGAACTGATTGGTGGCACTCATCGGACCAATCAGCAGTATCCTGAATTTGAAAACAACTACAACTGTGTTTACAAGAGAAA 1010 1020 1030 1040 1050 1060 1070 1080 1090 1100 1110 1120 1130 1140 1150 1160 1170 1180 1190 1200 AT5G21 GTTTGAAGAAGATTTTGGGATGCCAATGCATCCAACACTCCTAACATGTGAGAACAGTCTCTGTCCTTATAGCCAACCACATATGGGATTTCTTGACAGG ::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: :::::::::::::::::: IE_07F GTTTGAAGAAGATTTTGGGATGCCAATGCATCCAACACTCCTAACATGTGAGAACAGTCTCTGTCCTTATAGCCAACCACACATGGGATTTCTTGACAGG 1110 1120 1130 1140 1150 1160 1170 1180 1190 1200 1210 1220 1230 1240 1250 1260 1270 1280 1290 1300 AT5G21 AACTTAAGAGAGAATCACCAAATGACTTGTCCTTATAAAGTCACTTCCTTCTACCAACCAACTAAACCCTATGGTATGACGGGTTTAATGGTTCCTTGTC :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_07F AACTTAAGAGAGAATCACCAAATGACTTGTCCTTATAAAGTCACTTCCTTCTACCAACCAACTAAACCCTATGGTATGACGGGTTTAATGGTTCCTTGTC 1210 1220 1230 1240 1250 1260 1270 1280 1290 1300 1310 1320 1330 1340 1350 1360 1370 1380 1390 1400 AT5G21 CGGATTATAACGGGATGCAGCAGCAGGTTCAGAGCTTTCAAGACCAGTTTAATCATCCCAACGATCTCTACAGACCAAAAGCTCCACAAAGAGGCAACGA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_07F CGGATTATAACGGGATGCAGCAGCAGGTTCAGAGCTTTCAAGACCAGTTTAATCATCCCAACGATCTCTACAGACCAAAAGCTCCACAAAGAGGCAACGA 1310 1320 1330 1340 1350 1360 1370 1380 1390 1400 1410 1420 1430 1440 1450 1460 1470 1480 1490 1500 AT5G21 TGACTTGGTTGAGGATTTGAATCCTTCTCCTTCGACGCTGAATCAGAATCTTGGTTTAGTCTTACCTACTGACTTCAATGGAGGTGAGGAAACAGTAGGA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_07F TGACTTGGTTGAGGATTTGAATCCTTCTCCTTCGACGCTGAATCAGAATCTTGGTTTAGTCTTACCTACTGACTTCAATGGAGGTGAGGAAACAGTAGGA 1410 1420 1430 1440 1450 1460 1470 1480 1490 1500 1510 1520 1530 1540 1550 AT5G21 ACAGAGAACAATCTGCATAATCAAGGGCAAGAGTTGCCCACATCTTGGATTCAG :::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_07F ACAGAGAACAATCTGCATAATCAAGGGCAAGAGTTGCCCACATCTTGGATTCAG 1510 1520 1530 1540 1550 10 20 30 40 50 60 70 80 90 100 At5g21 MDMYNNNIGMFRSLVCSSAPPFTEGHMCSDSHTALCDDLSSDEEMEIEELEKKIWRDKQRLKRLKEMAKNGLGTRLLLKQQHDDFPEHSSKRTMYKAQDG :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_07F MDMYNNNIGMFRSLVCSSAPPFTEGHMCSDSHTALCDDLSSDEEMEIEELEKKIWRDKQRLKRLKEMAKNGLGTRLLLKQQHDDFPEHSSKRTMYKAQDG 10 20 30 40 50 60 70 80 90 100 110 120 130 140 150 160 170 180 190 200 At5g21 ILKYMSKTMERYKAQGFVYGIVLENGKTVAGSSDNLREWWKDKVRFDRNGPAAIIKHQRDINLSDGSDSGSEVGDSTAQKLLELQDTTLGALLSALFPHC :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_07F ILKYMSKTMERYKAQGFVYGIVLENGKTVAGSSDNLREWWKDKVRFDRNGPAAIIKHQRDINLSDGSDSGSEVGDSTAQKLLELQDTTLGALLSALFPHC 110 120 130 140 150 160 170 180 190 200 210 220 230 240 250 260 270 280 290 300 At5g21 NPPQRRFPLEKGVTPPWWPTGKEDWWDQLSLPVDFRGVPPPYKKPHDLKKLWKIGVLIGVIRHMASDISNIPNLVRRSRSLQEKMTSREGALWLAALYRE :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_07F NPPQRRFPLEKGVTPPWWPTGKEDWWDQLSLPVDFRGVPPPYKKPHDLKKLWKIGVLIGVIRHMASDISNIPNLVRRSRSLQEKMTSREGALWLAALYRE 210 220 230 240 250 260 270 280 290 300 310 320 330 340 350 360 370 380 390 400 At5g21 KAIVDQIAMSRENNNTSNFLVPATGGDPDVLFPESTDYDVELIGGTHRTNQQYPEFENNYNCVYKRKFEEDFGMPMHPTLLTCENSLCPYSQPHMGFLDR :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_07F KAIVDQIAMSRENNNTSNFLVPATGGDPDVLFPESTDYDVELIGGTHRTNQQYPEFENNYNCVYKRKFEEDFGMPMHPTLLTCENSLCPYSQPHMGFLDR 310 320 330 340 350 360 370 380 390 400 410 420 430 440 450 460 470 480 490 500 At5g21 NLRENHQMTCPYKVTSFYQPTKPYGMTGLMVPCPDYNGMQQQVQSFQDQFNHPNDLYRPKAPQRGNDDLVEDLNPSPSTLNQNLGLVLPTDFNGGEETVG :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_07F NLRENHQMTCPYKVTSFYQPTKPYGMTGLMVPCPDYNGMQQQVQSFQDQFNHPNDLYRPKAPQRGNDDLVEDLNPSPSTLNQNLGLVLPTDFNGGEETVG 410 420 430 440 450 460 470 480 490 500 510 At5g21 TENNLHNQGQELPTSWIQ :::::::::::::::::: IE_07F TENNLHNQGQELPTSWIQ 510

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âââIE_07F04 detailâââ