âââIE_07F05 detailâââ

Code name	IE_07F05
Locus	AT5G46760
Template	IT_07F05 (ABRC_223)
Donor Vector	pDONR207
Forward primer	IPF_07F05 (ggggacaagtttgtacaaaaaagcaggcttcATGAACGGCACAACATCATCAATCAAC)
Reverse primer	IPR_07F05 (ggggaccactttgtacaagaaagctgggttATAGTTTTCTCCGACTTTCGTCATCAAAGC)
Alignment with template seq.	1st	éåè§£æãè¡ããªãã£ããµã³ãã«ã§ãã	2nd	ã¢ããé¸éå100.00%ä¸è´
Alignment with TAIR7CDS	1st	-	2nd	ã¢ããé¸éå100.00%ä¸è´
comment	1st:(Sequenceè§£æã¾ã§è¡ã£ããããã¬ã¼ã ã¯ãããªããå¡©åºç½®æããã), 2nd:70%, nt.402G→A, nt685C→G, nt.1395G→A, nt.1596A→G
sequence	>IE_07F05_1776bp ATGAACGGCACAACATCATCAATCAACTTCTTGACCTCCGACGATGACGCGTCGGCGGCGGCTATGGAAGCTTTCATTGGAACAAACCACCACTCATCTC TCTTTCCTCCACCACCACAACAACCACCTCAGCCTCAGTTCAACGAAGATACTCTTCAACAACGTCTCCAAGCTTTAATAGAATCCGCCGGAGAAAACTG GACTTACGCTATCTTCTGGCAGATCTCACACGACTTCGATTCATCCACCGGAGATAACACAGTGATCCTCGGCTGGGGAGATGGTTACTACAAAGGAGAG GAAGATAAAGAGAAGAAGAAGAACAACACCAACACGGCGGAGCAAGAGCATCGGAAAAGAGTAATACGTGAGCTTAACTCGTTAATCTCCGGCGGAATTG GAGTTTCCGATGAATCAAACGATGAAGAAGTAACAGATACTGAATGGTTCTTCTTAGTTTCGATGACTCAAAGCTTCGTTAACGGTGTTGGTCTCCCCGG AGAATCTTTCTTAAACTCTCGTGTGATTTGGTTATCCGGGTCTGGTGCTTTAACCGGGTCGGGTTGTGAAAGAGCGGGTCAAGGTCAGATTTACGGGTTA AAGACGATGGTGTGTATCGCGACTCAAAACGGCGTCGTTGAGCTTGGTTCGTCGGAGGTTATAAGTCAAAGCTCAGATCTGATGGATAAAGTTAACAACT TGTTTAATTTCAACAACGGTGGTGGAAACAATGGTGTTGAAGCTTCTTCGTGGGGTTTTAATCTGAATCCAGATCAAGGAGAGAATGATCCAGCTTTGTG GATTAGTGAACCGACGAACACCGGAATCGAATCTCCGGCGAGGGTTAATAATGGTAATAACTCGAATTCTAATTCTAAGTCTGATTCTCATCAAATTTCT AAGCTTGAGAAGAATGATATTAGCTCTGTAGAGAATCAGAATCGTCAAAGTTCGTGTCTTGTCGAGAAAGATTTGACCTTTCAAGGTGGGTTGTTGAAAT CTAATGAGACTTTGAGTTTCTGTGGTAATGAGAGTAGTAAGAAGAGAACTTCGGTATCTAAAGGGAGTAATAATGATGAAGGGATGCTTTCGTTTAGTAC TGTGGTTAGATCAGCTGCGAATGATTCGGATCATTCTGATCTTGAAGCATCTGTTGTTAAGGAAGCGATTGTTGTTGAGCCACCGGAGAAGAAGCCGAGG AAACGGGGGAGGAAACCGGCGAATGGGAGAGAAGAGCCGTTGAATCATGTTGAAGCAGAGAGGCAGAGAAGAGAGAAGTTAAACCAGAGATTCTACTCTT TGAGAGCTGTTGTTCCTAACGTTTCGAAGATGGATAAAGCTTCGCTTCTCGGAGACGCGATTTCGTATATCAATGAGCTTAAGTCGAAGCTGCAACAAGC GGAGTCTGATAAAGAGGAGATTCAGAAGAAGCTAGATGGGATGAGTAAGGAAGGGAATAATGGGAAAGGTTGCGGGTCAAGGGCAAAAGAACGGAAAAGT TCGAATCAAGATTCTACGGCGAGTTCTATAGAAATGGAGATTGATGTTAAGATCATAGGTTGGGATGTGATGATACGTGTACAATGCGGCAAGAAGGATC ATCCCGGTGCTAGGTTCATGGAAGCACTTAAGGAATTGGATTTGGAAGTGAATCATGCGAGTTTATCCGTTGTGAATGATTTGATGATTCAACAAGCTAC GGTGAAGATGGGGAGCCAATTTTTCAATCATGACCAGCTCAAAGTTGCTTTGATGACGAAAGTCGGAGAAAACTAT >IE_07F05_translated MNGTTSSINFLTSDDDASAAAMEAFIGTNHHSSLFPPPPQQPPQPQFNEDTLQQRLQALIESAGENWTYAIFWQISHDFDSSTGDNTVILGWGDGYYKGE EDKEKKKNNTNTAEQEHRKRVIRELNSLISGGIGVSDESNDEEVTDTEWFFLVSMTQSFVNGVGLPGESFLNSRVIWLSGSGALTGSGCERAGQGQIYGL KTMVCIATQNGVVELGSSEVISQSSDLMDKVNNLFNFNNGGGNNGVEASSWGFNLNPDQGENDPALWISEPTNTGIESPARVNNGNNSNSNSKSDSHQIS KLEKNDISSVENQNRQSSCLVEKDLTFQGGLLKSNETLSFCGNESSKKRTSVSKGSNNDEGMLSFSTVVRSAANDSDHSDLEASVVKEAIVVEPPEKKPR KRGRKPANGREEPLNHVEAERQRREKLNQRFYSLRAVVPNVSKMDKASLLGDAISYINELKSKLQQAESDKEEIQKKLDGMSKEGNNGKGCGSRAKERKS SNQDSTASSIEMEIDVKIIGWDVMIRVQCGKKDHPGARFMEALKELDLEVNHASLSVVNDLMIQQATVKMGSQFFNHDQLKVALMTKVGENY
Alignment	◆CDS（TAIRver.9）_without_stop_codonとのalignment◆ 1772/1776 (99.8%)（アミノ酸配列で591/592 (99.8%)）一致しました。 10 20 30 40 50 60 70 80 90 100 AT5G46 ATGAACGGCACAACATCATCAATCAACTTCTTGACCTCCGACGATGACGCGTCGGCGGCGGCTATGGAAGCTTTCATTGGAACAAACCACCACTCATCTC :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_07F ATGAACGGCACAACATCATCAATCAACTTCTTGACCTCCGACGATGACGCGTCGGCGGCGGCTATGGAAGCTTTCATTGGAACAAACCACCACTCATCTC 10 20 30 40 50 60 70 80 90 100 110 120 130 140 150 160 170 180 190 200 AT5G46 TCTTTCCTCCACCACCACAACAACCACCTCAGCCTCAGTTCAACGAAGATACTCTTCAACAACGTCTCCAAGCTTTAATAGAATCCGCCGGAGAAAACTG :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_07F TCTTTCCTCCACCACCACAACAACCACCTCAGCCTCAGTTCAACGAAGATACTCTTCAACAACGTCTCCAAGCTTTAATAGAATCCGCCGGAGAAAACTG 110 120 130 140 150 160 170 180 190 200 210 220 230 240 250 260 270 280 290 300 AT5G46 GACTTACGCTATCTTCTGGCAGATCTCACACGACTTCGATTCATCCACCGGAGATAACACAGTGATCCTCGGCTGGGGAGATGGTTACTACAAAGGAGAG :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_07F GACTTACGCTATCTTCTGGCAGATCTCACACGACTTCGATTCATCCACCGGAGATAACACAGTGATCCTCGGCTGGGGAGATGGTTACTACAAAGGAGAG 210 220 230 240 250 260 270 280 290 300 310 320 330 340 350 360 370 380 390 400 AT5G46 GAAGATAAAGAGAAGAAGAAGAACAACACCAACACGGCGGAGCAAGAGCATCGGAAAAGAGTAATACGTGAGCTTAACTCGTTAATCTCCGGCGGAATTG :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_07F GAAGATAAAGAGAAGAAGAAGAACAACACCAACACGGCGGAGCAAGAGCATCGGAAAAGAGTAATACGTGAGCTTAACTCGTTAATCTCCGGCGGAATTG 310 320 330 340 350 360 370 380 390 400 410 420 430 440 450 460 470 480 490 500 AT5G46 GGGTTTCCGATGAATCAAACGATGAAGAAGTAACAGATACTGAATGGTTCTTCTTAGTTTCGATGACTCAAAGCTTCGTTAACGGTGTTGGTCTCCCCGG : :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_07F GAGTTTCCGATGAATCAAACGATGAAGAAGTAACAGATACTGAATGGTTCTTCTTAGTTTCGATGACTCAAAGCTTCGTTAACGGTGTTGGTCTCCCCGG 410 420 430 440 450 460 470 480 490 500 510 520 530 540 550 560 570 580 590 600 AT5G46 AGAATCTTTCTTAAACTCTCGTGTGATTTGGTTATCCGGGTCTGGTGCTTTAACCGGGTCGGGTTGTGAAAGAGCGGGTCAAGGTCAGATTTACGGGTTA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_07F AGAATCTTTCTTAAACTCTCGTGTGATTTGGTTATCCGGGTCTGGTGCTTTAACCGGGTCGGGTTGTGAAAGAGCGGGTCAAGGTCAGATTTACGGGTTA 510 520 530 540 550 560 570 580 590 600 610 620 630 640 650 660 670 680 690 700 AT5G46 AAGACGATGGTGTGTATCGCGACTCAAAACGGCGTCGTTGAGCTTGGTTCGTCGGAGGTTATAAGTCAAAGCTCAGATCTGATGCATAAAGTTAACAACT :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: ::::::::::::::: IE_07F AAGACGATGGTGTGTATCGCGACTCAAAACGGCGTCGTTGAGCTTGGTTCGTCGGAGGTTATAAGTCAAAGCTCAGATCTGATGGATAAAGTTAACAACT 610 620 630 640 650 660 670 680 690 700 710 720 730 740 750 760 770 780 790 800 AT5G46 TGTTTAATTTCAACAACGGTGGTGGAAACAATGGTGTTGAAGCTTCTTCGTGGGGTTTTAATCTGAATCCAGATCAAGGAGAGAATGATCCAGCTTTGTG :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_07F TGTTTAATTTCAACAACGGTGGTGGAAACAATGGTGTTGAAGCTTCTTCGTGGGGTTTTAATCTGAATCCAGATCAAGGAGAGAATGATCCAGCTTTGTG 710 720 730 740 750 760 770 780 790 800 810 820 830 840 850 860 870 880 890 900 AT5G46 GATTAGTGAACCGACGAACACCGGAATCGAATCTCCGGCGAGGGTTAATAATGGTAATAACTCGAATTCTAATTCTAAGTCTGATTCTCATCAAATTTCT :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_07F GATTAGTGAACCGACGAACACCGGAATCGAATCTCCGGCGAGGGTTAATAATGGTAATAACTCGAATTCTAATTCTAAGTCTGATTCTCATCAAATTTCT 810 820 830 840 850 860 870 880 890 900 910 920 930 940 950 960 970 980 990 1000 AT5G46 AAGCTTGAGAAGAATGATATTAGCTCTGTAGAGAATCAGAATCGTCAAAGTTCGTGTCTTGTCGAGAAAGATTTGACCTTTCAAGGTGGGTTGTTGAAAT :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_07F AAGCTTGAGAAGAATGATATTAGCTCTGTAGAGAATCAGAATCGTCAAAGTTCGTGTCTTGTCGAGAAAGATTTGACCTTTCAAGGTGGGTTGTTGAAAT 910 920 930 940 950 960 970 980 990 1000 1010 1020 1030 1040 1050 1060 1070 1080 1090 1100 AT5G46 CTAATGAGACTTTGAGTTTCTGTGGTAATGAGAGTAGTAAGAAGAGAACTTCGGTATCTAAAGGGAGTAATAATGATGAAGGGATGCTTTCGTTTAGTAC :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_07F CTAATGAGACTTTGAGTTTCTGTGGTAATGAGAGTAGTAAGAAGAGAACTTCGGTATCTAAAGGGAGTAATAATGATGAAGGGATGCTTTCGTTTAGTAC 1010 1020 1030 1040 1050 1060 1070 1080 1090 1100 1110 1120 1130 1140 1150 1160 1170 1180 1190 1200 AT5G46 TGTGGTTAGATCAGCTGCGAATGATTCGGATCATTCTGATCTTGAAGCATCTGTTGTTAAGGAAGCGATTGTTGTTGAGCCACCGGAGAAGAAGCCGAGG :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_07F TGTGGTTAGATCAGCTGCGAATGATTCGGATCATTCTGATCTTGAAGCATCTGTTGTTAAGGAAGCGATTGTTGTTGAGCCACCGGAGAAGAAGCCGAGG 1110 1120 1130 1140 1150 1160 1170 1180 1190 1200 1210 1220 1230 1240 1250 1260 1270 1280 1290 1300 AT5G46 AAACGGGGGAGGAAACCGGCGAATGGGAGAGAAGAGCCGTTGAATCATGTTGAAGCAGAGAGGCAGAGAAGAGAGAAGTTAAACCAGAGATTCTACTCTT :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_07F AAACGGGGGAGGAAACCGGCGAATGGGAGAGAAGAGCCGTTGAATCATGTTGAAGCAGAGAGGCAGAGAAGAGAGAAGTTAAACCAGAGATTCTACTCTT 1210 1220 1230 1240 1250 1260 1270 1280 1290 1300 1310 1320 1330 1340 1350 1360 1370 1380 1390 1400 AT5G46 TGAGAGCTGTTGTTCCTAACGTTTCGAAGATGGATAAAGCTTCGCTTCTCGGAGACGCGATTTCGTATATCAATGAGCTTAAGTCGAAGCTGCAGCAAGC :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: ::::: IE_07F TGAGAGCTGTTGTTCCTAACGTTTCGAAGATGGATAAAGCTTCGCTTCTCGGAGACGCGATTTCGTATATCAATGAGCTTAAGTCGAAGCTGCAACAAGC 1310 1320 1330 1340 1350 1360 1370 1380 1390 1400 1410 1420 1430 1440 1450 1460 1470 1480 1490 1500 AT5G46 GGAGTCTGATAAAGAGGAGATTCAGAAGAAGCTAGATGGGATGAGTAAGGAAGGGAATAATGGGAAAGGTTGCGGGTCAAGGGCAAAAGAACGGAAAAGT :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_07F GGAGTCTGATAAAGAGGAGATTCAGAAGAAGCTAGATGGGATGAGTAAGGAAGGGAATAATGGGAAAGGTTGCGGGTCAAGGGCAAAAGAACGGAAAAGT 1410 1420 1430 1440 1450 1460 1470 1480 1490 1500 1510 1520 1530 1540 1550 1560 1570 1580 1590 1600 AT5G46 TCGAATCAAGATTCTACGGCGAGTTCTATAGAAATGGAGATTGATGTTAAGATCATAGGTTGGGATGTGATGATACGTGTACAATGCGGCAAGAAAGATC ::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: :::: IE_07F TCGAATCAAGATTCTACGGCGAGTTCTATAGAAATGGAGATTGATGTTAAGATCATAGGTTGGGATGTGATGATACGTGTACAATGCGGCAAGAAGGATC 1510 1520 1530 1540 1550 1560 1570 1580 1590 1600 1610 1620 1630 1640 1650 1660 1670 1680 1690 1700 AT5G46 ATCCCGGTGCTAGGTTCATGGAAGCACTTAAGGAATTGGATTTGGAAGTGAATCATGCGAGTTTATCCGTTGTGAATGATTTGATGATTCAACAAGCTAC :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_07F ATCCCGGTGCTAGGTTCATGGAAGCACTTAAGGAATTGGATTTGGAAGTGAATCATGCGAGTTTATCCGTTGTGAATGATTTGATGATTCAACAAGCTAC 1610 1620 1630 1640 1650 1660 1670 1680 1690 1700 1710 1720 1730 1740 1750 1760 1770 AT5G46 GGTGAAGATGGGGAGCCAATTTTTCAATCATGACCAGCTCAAAGTTGCTTTGATGACGAAAGTCGGAGAAAACTAT :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_07F GGTGAAGATGGGGAGCCAATTTTTCAATCATGACCAGCTCAAAGTTGCTTTGATGACGAAAGTCGGAGAAAACTAT 1710 1720 1730 1740 1750 1760 1770 10 20 30 40 50 60 70 80 90 100 AT5G46 MNGTTSSINFLTSDDDASAAAMEAFIGTNHHSSLFPPPPQQPPQPQFNEDTLQQRLQALIESAGENWTYAIFWQISHDFDSSTGDNTVILGWGDGYYKGE :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_07F MNGTTSSINFLTSDDDASAAAMEAFIGTNHHSSLFPPPPQQPPQPQFNEDTLQQRLQALIESAGENWTYAIFWQISHDFDSSTGDNTVILGWGDGYYKGE 10 20 30 40 50 60 70 80 90 100 110 120 130 140 150 160 170 180 190 200 AT5G46 EDKEKKKNNTNTAEQEHRKRVIRELNSLISGGIGVSDESNDEEVTDTEWFFLVSMTQSFVNGVGLPGESFLNSRVIWLSGSGALTGSGCERAGQGQIYGL :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_07F EDKEKKKNNTNTAEQEHRKRVIRELNSLISGGIGVSDESNDEEVTDTEWFFLVSMTQSFVNGVGLPGESFLNSRVIWLSGSGALTGSGCERAGQGQIYGL 110 120 130 140 150 160 170 180 190 200 210 220 230 240 250 260 270 280 290 300 AT5G46 KTMVCIATQNGVVELGSSEVISQSSDLMHKVNNLFNFNNGGGNNGVEASSWGFNLNPDQGENDPALWISEPTNTGIESPARVNNGNNSNSNSKSDSHQIS :::::::::::::::::::::::::::: ::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_07F KTMVCIATQNGVVELGSSEVISQSSDLMDKVNNLFNFNNGGGNNGVEASSWGFNLNPDQGENDPALWISEPTNTGIESPARVNNGNNSNSNSKSDSHQIS 210 220 230 240 250 260 270 280 290 300 310 320 330 340 350 360 370 380 390 400 AT5G46 KLEKNDISSVENQNRQSSCLVEKDLTFQGGLLKSNETLSFCGNESSKKRTSVSKGSNNDEGMLSFSTVVRSAANDSDHSDLEASVVKEAIVVEPPEKKPR :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_07F KLEKNDISSVENQNRQSSCLVEKDLTFQGGLLKSNETLSFCGNESSKKRTSVSKGSNNDEGMLSFSTVVRSAANDSDHSDLEASVVKEAIVVEPPEKKPR 310 320 330 340 350 360 370 380 390 400 410 420 430 440 450 460 470 480 490 500 AT5G46 KRGRKPANGREEPLNHVEAERQRREKLNQRFYSLRAVVPNVSKMDKASLLGDAISYINELKSKLQQAESDKEEIQKKLDGMSKEGNNGKGCGSRAKERKS :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_07F KRGRKPANGREEPLNHVEAERQRREKLNQRFYSLRAVVPNVSKMDKASLLGDAISYINELKSKLQQAESDKEEIQKKLDGMSKEGNNGKGCGSRAKERKS 410 420 430 440 450 460 470 480 490 500 510 520 530 540 550 560 570 580 590 AT5G46 SNQDSTASSIEMEIDVKIIGWDVMIRVQCGKKDHPGARFMEALKELDLEVNHASLSVVNDLMIQQATVKMGSQFFNHDQLKVALMTKVGENY :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_07F SNQDSTASSIEMEIDVKIIGWDVMIRVQCGKKDHPGARFMEALKELDLEVNHASLSVVNDLMIQQATVKMGSQFFNHDQLKVALMTKVGENY 510 520 530 540 550 560 570 580 590

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âââIE_07F05 detailâââ

âââIE_07F05 detailâââ