◆◆◆IE_07F07 detail◆◆◆

Code name	IE_07F07
Locus	AT5G18560
Template	IT_07F07 (CR018)
Donor Vector	pDONR207
Forward primer	IPF_07F07 (ggggacaagtttgtacaaaaaagcaggcttcATGGGTTTTGCTCTGATCCAC)
Reverse primer	IPR_07F07 (ggggaccactttgtacaagaaagctgggttAAAGACTGAGTAGAAGCCTGTAGTGTCTGC)
Alignment with template seq.	1st	アミノ酸配列100%一致	2nd	-
Alignment with TAIR7CDS	1st	アミノ酸配列94.50%一致	2nd	-
comment	1st:Templateの配列とは一致
sequence	>IE_07F07_987bp ATGGGTTTTGCTCTGATCCACCAAAACACATCCGCCAACACAACCACCACCACCACAACCGGCGAGAGACGAGGCCGGAGATCAAAGCAAGCGGAGCCAG GGAGGTTCCTAGGAGTCCGGAGACGTCCATGGGGTCGTTACGCAGCCGAGATAAGAGATCCAACCACCAAAGAACGTCACTGGCTAGGCACTTTCGACAC AGCTCACGAAGCTGCTCTTGCCTACGATAGAGCTGCTCTTTCAATGAGAGGTACTCAAGCTCGAACCAACTTCGTCTACACTCCAACTGATGTCCACACC ATCCTCACAAACCCTAATCTCCACTCTCTCATTGTCTCTCCTTACAATAACAACCAATCTTTCTTACCAAACTCTTCTCCACAGTTTGTCATCGATCATC ATCCTCATTATCAAAACTATCATCAACCACAACAACCTAAACACACTCTCCCCCAAACTGTCTTACCCGCGGCTTCTTTCAAAACGCCAGTTCGTCATCA AAGCGTGGATATTCAAGCGTTTGGTAATAGTCCTCAAAACTCATCTTCTAACGGCTCGTTATCTTCTTCACTAGACGAAGAAAACAACTTCTTCTTCTCT TTAACAAGCGAAGAACATAATAAGTCCAATAACAACTCGGGTTACTTGGATTGCATTGTCCCAAACCACTGCCTTAAACCACCTCCGGAGGCCACCACCA CCCAAAATCAAGCCGGAGCTAGTTTCACAACTCCGGTAGCAAGCAAAGCCTCTGAACCATATGGAGGGTTTAGTAATAGCTACTTCGAAGATGGTGAGAT GATGATGATGAATCATCATGAGTTTGGTTCGTGTGATCTCTCTGCCATGATCACGAACTATGGTGCTGCTGCCGCTTCAATGTCAATGGAAGATTACGGG ATGATGGAGCCGCAAGATTTATCTTCTTCTTCTATCGCTGCTTTTGGAGATGTCGTTGCAGACACTACAGGCTTCTACTCAGTCTTT >IE_07F07_translated MGFALIHQNTSANTTTTTTTGERRGRRSKQAEPGRFLGVRRRPWGRYAAEIRDPTTKERHWLGTFDTAHEAALAYDRAALSMRGTQARTNFVYTPTDVHT ILTNPNLHSLIVSPYNNNQSFLPNSSPQFVIDHHPHYQNYHQPQQPKHTLPQTVLPAASFKTPVRHQSVDIQAFGNSPQNSSSNGSLSSSLDEENNFFFS LTSEEHNKSNNNSGYLDCIVPNHCLKPPPEATTTQNQAGASFTTPVASKASEPYGGFSNSYFEDGEMMMMNHHEFGSCDLSAMITNYGAAAASMSMEDYG MMEPQDLSSSSIAAFGDVVADTTGFYSVF
Alignment	◆CDS（TAIRver.9）_without_stop_codonとのalignment◆ 987/1044 (94.5%)（アミノ酸配列で329/348 (94.5%)）一致しました。 10 20 30 40 50 60 70 80 90 100 AT5G18 ATGTCAACCTCCAAAACCCTAGACCATAATAAACCATTTGAAACATCCCAAACGCAAATGGGTTTTGCTCTGATCCACCAAAACACATCCGCCAACACAA ::::::::::::::::::::::::::::::::::::::::::: IE_07F ---------------------------------------------------------ATGGGTTTTGCTCTGATCCACCAAAACACATCCGCCAACACAA 10 20 30 40 110 120 130 140 150 160 170 180 190 200 AT5G18 CCACCACCACCACAACCGGCGAGAGACGAGGCCGGAGATCAAAGCAAGCGGAGCCAGGGAGGTTCCTAGGAGTCCGGAGACGTCCATGGGGTCGTTACGC :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_07F CCACCACCACCACAACCGGCGAGAGACGAGGCCGGAGATCAAAGCAAGCGGAGCCAGGGAGGTTCCTAGGAGTCCGGAGACGTCCATGGGGTCGTTACGC 50 60 70 80 90 100 110 120 130 140 210 220 230 240 250 260 270 280 290 300 AT5G18 AGCCGAGATAAGAGATCCAACCACCAAAGAACGTCACTGGCTAGGCACTTTCGACACAGCTCACGAAGCTGCTCTTGCCTACGATAGAGCTGCTCTTTCA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_07F AGCCGAGATAAGAGATCCAACCACCAAAGAACGTCACTGGCTAGGCACTTTCGACACAGCTCACGAAGCTGCTCTTGCCTACGATAGAGCTGCTCTTTCA 150 160 170 180 190 200 210 220 230 240 310 320 330 340 350 360 370 380 390 400 AT5G18 ATGAGAGGTACTCAAGCTCGAACCAACTTCGTCTACACTCCAACTGATGTCCACACCATCCTCACAAACCCTAATCTCCACTCTCTCATTGTCTCTCCTT :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_07F ATGAGAGGTACTCAAGCTCGAACCAACTTCGTCTACACTCCAACTGATGTCCACACCATCCTCACAAACCCTAATCTCCACTCTCTCATTGTCTCTCCTT 250 260 270 280 290 300 310 320 330 340 410 420 430 440 450 460 470 480 490 500 AT5G18 ACAATAACAACCAATCTTTCTTACCAAACTCTTCTCCACAGTTTGTCATCGATCATCATCCTCATTATCAAAACTATCATCAACCACAACAACCTAAACA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_07F ACAATAACAACCAATCTTTCTTACCAAACTCTTCTCCACAGTTTGTCATCGATCATCATCCTCATTATCAAAACTATCATCAACCACAACAACCTAAACA 350 360 370 380 390 400 410 420 430 440 510 520 530 540 550 560 570 580 590 600 AT5G18 CACTCTCCCCCAAACTGTCTTACCCGCGGCTTCTTTCAAAACGCCAGTTCGTCATCAAAGCGTGGATATTCAAGCGTTTGGTAATAGTCCTCAAAACTCA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_07F CACTCTCCCCCAAACTGTCTTACCCGCGGCTTCTTTCAAAACGCCAGTTCGTCATCAAAGCGTGGATATTCAAGCGTTTGGTAATAGTCCTCAAAACTCA 450 460 470 480 490 500 510 520 530 540 610 620 630 640 650 660 670 680 690 700 AT5G18 TCTTCTAACGGCTCGTTATCTTCTTCACTAGACGAAGAAAACAACTTCTTCTTCTCTTTAACAAGCGAAGAACATAATAAGTCCAATAACAACTCGGGTT :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_07F TCTTCTAACGGCTCGTTATCTTCTTCACTAGACGAAGAAAACAACTTCTTCTTCTCTTTAACAAGCGAAGAACATAATAAGTCCAATAACAACTCGGGTT 550 560 570 580 590 600 610 620 630 640 710 720 730 740 750 760 770 780 790 800 AT5G18 ACTTGGATTGCATTGTCCCAAACCACTGCCTTAAACCACCTCCGGAGGCCACCACCACCCAAAATCAAGCCGGAGCTAGTTTCACAACTCCGGTAGCAAG :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_07F ACTTGGATTGCATTGTCCCAAACCACTGCCTTAAACCACCTCCGGAGGCCACCACCACCCAAAATCAAGCCGGAGCTAGTTTCACAACTCCGGTAGCAAG 650 660 670 680 690 700 710 720 730 740 810 820 830 840 850 860 870 880 890 900 AT5G18 CAAAGCCTCTGAACCATATGGAGGGTTTAGTAATAGCTACTTCGAAGATGGTGAGATGATGATGATGAATCATCATGAGTTTGGTTCGTGTGATCTCTCT :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_07F CAAAGCCTCTGAACCATATGGAGGGTTTAGTAATAGCTACTTCGAAGATGGTGAGATGATGATGATGAATCATCATGAGTTTGGTTCGTGTGATCTCTCT 750 760 770 780 790 800 810 820 830 840 910 920 930 940 950 960 970 980 990 1000 AT5G18 GCCATGATCACGAACTATGGTGCTGCTGCCGCTTCAATGTCAATGGAAGATTACGGGATGATGGAGCCGCAAGATTTATCTTCTTCTTCTATCGCTGCTT :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_07F GCCATGATCACGAACTATGGTGCTGCTGCCGCTTCAATGTCAATGGAAGATTACGGGATGATGGAGCCGCAAGATTTATCTTCTTCTTCTATCGCTGCTT 850 860 870 880 890 900 910 920 930 940 1010 1020 1030 1040 AT5G18 TTGGAGATGTCGTTGCAGACACTACAGGCTTCTACTCAGTCTTT :::::::::::::::::::::::::::::::::::::::::::: IE_07F TTGGAGATGTCGTTGCAGACACTACAGGCTTCTACTCAGTCTTT 950 960 970 980 10 20 30 40 50 60 70 80 90 100 AT5G18 MSTSKTLDHNKPFETSQTQMGFALIHQNTSANTTTTTTTGERRGRRSKQAEPGRFLGVRRRPWGRYAAEIRDPTTKERHWLGTFDTAHEAALAYDRAALS ::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_07F -------------------MGFALIHQNTSANTTTTTTTGERRGRRSKQAEPGRFLGVRRRPWGRYAAEIRDPTTKERHWLGTFDTAHEAALAYDRAALS 10 20 30 40 50 60 70 80 110 120 130 140 150 160 170 180 190 200 AT5G18 MRGTQARTNFVYTPTDVHTILTNPNLHSLIVSPYNNNQSFLPNSSPQFVIDHHPHYQNYHQPQQPKHTLPQTVLPAASFKTPVRHQSVDIQAFGNSPQNS :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_07F MRGTQARTNFVYTPTDVHTILTNPNLHSLIVSPYNNNQSFLPNSSPQFVIDHHPHYQNYHQPQQPKHTLPQTVLPAASFKTPVRHQSVDIQAFGNSPQNS 90 100 110 120 130 140 150 160 170 180 210 220 230 240 250 260 270 280 290 300 AT5G18 SSNGSLSSSLDEENNFFFSLTSEEHNKSNNNSGYLDCIVPNHCLKPPPEATTTQNQAGASFTTPVASKASEPYGGFSNSYFEDGEMMMMNHHEFGSCDLS :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_07F SSNGSLSSSLDEENNFFFSLTSEEHNKSNNNSGYLDCIVPNHCLKPPPEATTTQNQAGASFTTPVASKASEPYGGFSNSYFEDGEMMMMNHHEFGSCDLS 190 200 210 220 230 240 250 260 270 280 310 320 330 340 AT5G18 AMITNYGAAAASMSMEDYGMMEPQDLSSSSIAAFGDVVADTTGFYSVF :::::::::::::::::::::::::::::::::::::::::::::::: IE_07F AMITNYGAAAASMSMEDYGMMEPQDLSSSSIAAFGDVVADTTGFYSVF 290 300 310 320

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