| Code name | IE_07F11 | |||
|---|---|---|---|---|
| Locus | AT1G01010 | |||
| Template | IT_07F11 (CR215) | |||
| Donor Vector | pDONR207 | |||
| Forward primer | IPF_07F11 (ggggacaagtttgtacaaaaaagcaggcttcATGGAGGATCAAGTTGGGTTTGGGTTC) | |||
| Reverse primer | IPR_07F11 (ggggaccactttgtacaagaaagctgggttACCAACAAGAATGATCCAACTAATGACGGA) | |||
| Alignment with template seq. | 1st | 配列解析が行えなかったサンプルです。 | 2nd | アミノ酸配列100%一致 |
| Alignment with TAIR7CDS | - | アミノ酸配列100%一致 | ||
| comment | 1st:(Sequence解析まで行ったが、読み直す必要がある) | |||
| sequence |
>IE_07F11_1287bp ATGGAGGATCAAGTTGGGTTTGGGTTCCGTCCGAACGACGAGGAGCTCGTTGGTCACTATCTCCGTAACAAAATCGAAGGAAACACTAGCCGCGACGTTG AAGTAGCCATCAGCGAGGTCAACATCTGTAGCTACGATCCTTGGAACTTGCGCTTCCAGTCAAAGTACAAATCGAGAGATGCTATGTGGTACTTCTTCTC TCGTAGAGAAAACAACAAAGGGAATCGACAGAGCAGGACAACGGTTTCTGGTAAATGGAAGCTTACCGGAGAATCTGTTGAGGTCAAGGACCAGTGGGGA TTTTGTAGTGAGGGCTTTCGTGGTAAGATTGGTCATAAAAGGGTTTTGGTGTTCCTCGATGGAAGATACCCTGACAAAACCAAATCTGATTGGGTTATCC ACGAGTTCCACTACGACCTCTTACCAGAACATCAGAGGACATATGTCATCTGCAGACTTGAGTACAAGGGTGATGATGCGGACATTCTATCTGCTTATGC AATAGATCCCACTCCCGCTTTTGTCCCCAATATGACTAGTAGTGCAGGTTCTGTGGTCAACCAATCACGTCAACGAAATTCAGGATCTTACAACACTTAC TCTGAGTATGATTCAGCAAATCATGGCCAGCAGTTTAATGAAAACTCTAACATTATGCAGCAGCAACCACTTCAAGGATCATTCAACCCTCTCCTTGAGT ATGATTTTGCAAATCACGGCGGTCAGTGGCTGAGTGACTATATCGACCTGCAACAGCAAGTTCCTTACTTGGCACCTTATGAAAATGAGTCGGAGATGAT TTGGAAGCATGTGATTGAAGAAAATTTTGAGTTTTTGGTAGATGAAAGGACATCTATGCAACAGCATTACAGTGATCACCGGCCCAAAAAACCTGTGTCT GGGGTTTTGCCTGATGATAGCAGTGATACTGAAACTGGATCAATGATTTTCGAAGACACTTCGAGCTCCACTGATAGTGTTGGTAGTTCAGATGAACCGG GCCATACTCGTATAGATGATATTCCATCATTGAACATTATTGAGCCTTTGCACAATTATAAGGCACAAGAGCAACCAAAGCAGCAGAGCAAAGAAAAGGT GATAAGTTCGCAGAAAAGCGAATGCGAGTGGAAAATGGCTGAAGACTCGATCAAGATACCTCCATCCACCAACACGGTGAAGCAGAGCTGGATTGTTTTG GAGAATGCACAGTGGAACTATCTCAAGAACATGATCATTGGTGTCTTGTTGTTCATCTCCGTCATTAGTTGGATCATTCTTGTTGGT >IE_07F11_translated MEDQVGFGFRPNDEELVGHYLRNKIEGNTSRDVEVAISEVNICSYDPWNLRFQSKYKSRDAMWYFFSRRENNKGNRQSRTTVSGKWKLTGESVEVKDQWG FCSEGFRGKIGHKRVLVFLDGRYPDKTKSDWVIHEFHYDLLPEHQRTYVICRLEYKGDDADILSAYAIDPTPAFVPNMTSSAGSVVNQSRQRNSGSYNTY SEYDSANHGQQFNENSNIMQQQPLQGSFNPLLEYDFANHGGQWLSDYIDLQQQVPYLAPYENESEMIWKHVIEENFEFLVDERTSMQQHYSDHRPKKPVS GVLPDDSSDTETGSMIFEDTSSSTDSVGSSDEPGHTRIDDIPSLNIIEPLHNYKAQEQPKQQSKEKVISSQKSECEWKMAEDSIKIPPSTNTVKQSWIVL ENAQWNYLKNMIIGVLLFISVISWIILVG |
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| Alignment |
◆CDS（TAIRver.9）_without_stop_codonとのalignment◆ 100%一致しました。 |
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