◆◆◆IE_07G03 detail◆◆◆

Code nameIE_07G03
LocusAT3G44290
TemplateIT_07G03 (CR236)
Donor VectorpDONR207
Forward primerIPF_07G03 (ggggacaagtttgtacaaaaaagcaggcttcATGGCAGCTGCACCACCGATCGAGCCG)
Reverse primerIPR_07G03 (ggggaccactttgtacaagaaagctgggttCACTAAATAAAACACCAACACGGCTATCAT)
Alignment with template seq.1stアミノ酸配列99.70%一致2ndアミノ酸配列99.70%一致
Alignment with TAIR7CDSアミノ酸配列99.70%一致アミノ酸配列99.70%一致
comment1st:(Sequence解析まで行ったが、フレームはずれないが塩基置換がある), 2nd:70%, nt.63C→G, nt.78A→G, nt.374C→G, nt.504A→G, nt.894C→T
sequence 
>IE_07G03_1005bp
ATGGCAGCTGCACCACCGATCGAGCCGGCGGTGACGACGACGTTCCCTGGCTTCAAATTCTCGCCGACCGACATCGAGCTTATATCTTATTACTTGAAGA
GGAAGATGGATGGTTTGGAGAGATCTGTTGAGATTATACCTGAGGTTGAGATTTACAACTTCGAGCCTTGGGATTTACCTGATAAGTCTATCGTTAAATC
GGATAGCGAATGGTTCTTCTTCTGTGCTCGCGGTAAGAAGTATCCCCACGGTTCACAGAACAGAAGAGCAACCAAGATAGGATATTGGAAAGCCACAGGA
AAAGAGCGTAATGTGAAGTCAGGTTCTGAGGTCATTGGAACAAAGAGAACGCTTGTATTCCACATTGGCCGCGGGCCAAAAGGAGGAAGAACGGAATGGC
TTATGCATGAGTACTGCATGATTGGAGTATCACTGGATGCTCTGGTTATTTGCCGACTTAGGAGGAATACTGAATTTCAAGGGAGTACAATTCAAAAGCC
ACCGCAACCAAGTTTACCACTGGATAAGCACGTAAACTTGCGGAATGAAGCTATTTCAGAAAGTATTTATGGTTGGGAAACTATGGTTGATTTCTACTTA
TCAAGTGAGTCAGGGCAAGAACTACTTAGTGAAATAGCAGAATCTTCACAATCTTCACAAAATCCACAGGTTCCTAGTGAAGAAGATTTCTATGCCGATA
TACTGAGGGATGAAATAGTAAAGCTAGATGATCCGGCGGTATCCGGTAATACACTGATCAATGTACCAAGGCTTCAAACAGAGTCCAATACCACAAGAGT
ACTACCTTTACCTGACATGGTAGACAAACAAATGCAATCGTTGCTACAGAAACTACCATTACAAAATGACACTGGAGAAGAGAACAACATATCTATGTCA
AATTGCTTTATCGGTATCTACTCGATTAAGTCTATAAACCGAGCGCGATGGGACGTTGTTGTTTGGTTACTCGTTATGATAGCCGTGTTGGTGTTTTATT
TAGTG

>IE_07G03_translated
MAAAPPIEPAVTTTFPGFKFSPTDIELISYYLKRKMDGLERSVEIIPEVEIYNFEPWDLPDKSIVKSDSEWFFFCARGKKYPHGSQNRRATKIGYWKATG
KERNVKSGSEVIGTKRTLVFHIGRGPKGGRTEWLMHEYCMIGVSLDALVICRLRRNTEFQGSTIQKPPQPSLPLDKHVNLRNEAISESIYGWETMVDFYL
SSESGQELLSEIAESSQSSQNPQVPSEEDFYADILRDEIVKLDDPAVSGNTLINVPRLQTESNTTRVLPLPDMVDKQMQSLLQKLPLQNDTGEENNISMS
NCFIGIYSIKSINRARWDVVVWLLVMIAVLVFYLV
Alignment ◆CDS（TAIRver.9）_without_stop_codonとのalignment◆

1000/1005 (99.5%)（アミノ酸配列で334/335 (99.7%)）一致しました。


               10        20        30        40        50        60        70        80        90       100
AT3G44 ATGGCAGCTGCACCACCGATCGAGCCGGCGGTGACGACGACGTTCCCTGGCTTCAAATTCTCCCCGACCGACATCGAACTTATATCTTATTACTTGAAGA
       :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: :::::::::::::: ::::::::::::::::::::::
IE_07G ATGGCAGCTGCACCACCGATCGAGCCGGCGGTGACGACGACGTTCCCTGGCTTCAAATTCTCGCCGACCGACATCGAGCTTATATCTTATTACTTGAAGA
               10        20        30        40        50        60        70        80        90       100

              110       120       130       140       150       160       170       180       190       200
AT3G44 GGAAGATGGATGGTTTGGAGAGATCTGTTGAGATTATACCTGAGGTTGAGATTTACAACTTCGAGCCTTGGGATTTACCTGATAAGTCTATCGTTAAATC
       ::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_07G GGAAGATGGATGGTTTGGAGAGATCTGTTGAGATTATACCTGAGGTTGAGATTTACAACTTCGAGCCTTGGGATTTACCTGATAAGTCTATCGTTAAATC
              110       120       130       140       150       160       170       180       190       200

              210       220       230       240       250       260       270       280       290       300
AT3G44 GGATAGCGAATGGTTCTTCTTCTGTGCTCGCGGTAAGAAGTATCCCCACGGTTCACAGAACAGAAGAGCAACCAAGATAGGATATTGGAAAGCCACAGGA
       ::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_07G GGATAGCGAATGGTTCTTCTTCTGTGCTCGCGGTAAGAAGTATCCCCACGGTTCACAGAACAGAAGAGCAACCAAGATAGGATATTGGAAAGCCACAGGA
              210       220       230       240       250       260       270       280       290       300

              310       320       330       340       350       360       370       380       390       400
AT3G44 AAAGAGCGTAATGTGAAGTCAGGTTCTGAGGTCATTGGAACAAAGAGAACGCTTGTATTCCACATTGGCCGCGCGCCAAAAGGAGGAAGAACGGAATGGC
       ::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: ::::::::::::::::::::::::::
IE_07G AAAGAGCGTAATGTGAAGTCAGGTTCTGAGGTCATTGGAACAAAGAGAACGCTTGTATTCCACATTGGCCGCGGGCCAAAAGGAGGAAGAACGGAATGGC
              310       320       330       340       350       360       370       380       390       400

              410       420       430       440       450       460       470       480       490       500
AT3G44 TTATGCATGAGTACTGCATGATTGGAGTATCACTGGATGCTCTGGTTATTTGCCGACTTAGGAGGAATACTGAATTTCAAGGGAGTACAATTCAAAAGCC
       ::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_07G TTATGCATGAGTACTGCATGATTGGAGTATCACTGGATGCTCTGGTTATTTGCCGACTTAGGAGGAATACTGAATTTCAAGGGAGTACAATTCAAAAGCC
              410       420       430       440       450       460       470       480       490       500

              510       520       530       540       550       560       570       580       590       600
AT3G44 ACCACAACCAAGTTTACCACTGGATAAGCACGTAAACTTGCGGAATGAAGCTATTTCAGAAAGTATTTATGGTTGGGAAACTATGGTTGATTTCTACTTA
       ::: ::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_07G ACCGCAACCAAGTTTACCACTGGATAAGCACGTAAACTTGCGGAATGAAGCTATTTCAGAAAGTATTTATGGTTGGGAAACTATGGTTGATTTCTACTTA
              510       520       530       540       550       560       570       580       590       600

              610       620       630       640       650       660       670       680       690       700
AT3G44 TCAAGTGAGTCAGGGCAAGAACTACTTAGTGAAATAGCAGAATCTTCACAATCTTCACAAAATCCACAGGTTCCTAGTGAAGAAGATTTCTATGCCGATA
       ::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_07G TCAAGTGAGTCAGGGCAAGAACTACTTAGTGAAATAGCAGAATCTTCACAATCTTCACAAAATCCACAGGTTCCTAGTGAAGAAGATTTCTATGCCGATA
              610       620       630       640       650       660       670       680       690       700

              710       720       730       740       750       760       770       780       790       800
AT3G44 TACTGAGGGATGAAATAGTAAAGCTAGATGATCCGGCGGTATCCGGTAATACACTGATCAATGTACCAAGGCTTCAAACAGAGTCCAATACCACAAGAGT
       ::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_07G TACTGAGGGATGAAATAGTAAAGCTAGATGATCCGGCGGTATCCGGTAATACACTGATCAATGTACCAAGGCTTCAAACAGAGTCCAATACCACAAGAGT
              710       720       730       740       750       760       770       780       790       800

              810       820       830       840       850       860       870       880       890       900
AT3G44 ACTACCTTTACCTGACATGGTAGACAAACAAATGCAATCGTTGCTACAGAAACTACCATTACAAAATGACACTGGAGAAGAGAACAACATATCCATGTCA
       ::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: ::::::
IE_07G ACTACCTTTACCTGACATGGTAGACAAACAAATGCAATCGTTGCTACAGAAACTACCATTACAAAATGACACTGGAGAAGAGAACAACATATCTATGTCA
              810       820       830       840       850       860       870       880       890       900

              910       920       930       940       950       960       970       980       990      1000
AT3G44 AATTGCTTTATCGGTATCTACTCGATTAAGTCTATAAACCGAGCGCGATGGGACGTTGTTGTTTGGTTACTCGTTATGATAGCCGTGTTGGTGTTTTATT
       ::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_07G AATTGCTTTATCGGTATCTACTCGATTAAGTCTATAAACCGAGCGCGATGGGACGTTGTTGTTTGGTTACTCGTTATGATAGCCGTGTTGGTGTTTTATT
              910       920       930       940       950       960       970       980       990      1000

            
AT3G44 TAGTG
       :::::
IE_07G TAGTG
            




               10        20        30        40        50        60        70        80        90       100
AT3G44 MAAAPPIEPAVTTTFPGFKFSPTDIELISYYLKRKMDGLERSVEIIPEVEIYNFEPWDLPDKSIVKSDSEWFFFCARGKKYPHGSQNRRATKIGYWKATG
       ::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_07G MAAAPPIEPAVTTTFPGFKFSPTDIELISYYLKRKMDGLERSVEIIPEVEIYNFEPWDLPDKSIVKSDSEWFFFCARGKKYPHGSQNRRATKIGYWKATG
               10        20        30        40        50        60        70        80        90       100

              110       120       130       140       150       160       170       180       190       200
AT3G44 KERNVKSGSEVIGTKRTLVFHIGRAPKGGRTEWLMHEYCMIGVSLDALVICRLRRNTEFQGSTIQKPPQPSLPLDKHVNLRNEAISESIYGWETMVDFYL
       :::::::::::::::::::::::: :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_07G KERNVKSGSEVIGTKRTLVFHIGRGPKGGRTEWLMHEYCMIGVSLDALVICRLRRNTEFQGSTIQKPPQPSLPLDKHVNLRNEAISESIYGWETMVDFYL
              110       120       130       140       150       160       170       180       190       200

              210       220       230       240       250       260       270       280       290       300
AT3G44 SSESGQELLSEIAESSQSSQNPQVPSEEDFYADILRDEIVKLDDPAVSGNTLINVPRLQTESNTTRVLPLPDMVDKQMQSLLQKLPLQNDTGEENNISMS
       ::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_07G SSESGQELLSEIAESSQSSQNPQVPSEEDFYADILRDEIVKLDDPAVSGNTLINVPRLQTESNTTRVLPLPDMVDKQMQSLLQKLPLQNDTGEENNISMS
              210       220       230       240       250       260       270       280       290       300

              310       320       330     
AT3G44 NCFIGIYSIKSINRARWDVVVWLLVMIAVLVFYLV
       :::::::::::::::::::::::::::::::::::
IE_07G NCFIGIYSIKSINRARWDVVVWLLVMIAVLVFYLV
              310       320       330

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