◆◆◆IE_07G09（IE_09C10） detail◆◆◆

Code nameIE_07G09（IE_09C10）
LocusAT4G13620
TemplateIT_07G09 (CR551)
Donor VectorpDONR207
Forward primerIPF_07G09 (ggggacaagtttgtacaaaaaagcaggcttcATGATCACACCAATACACACACAACAC)
Reverse primerIPR_07G09 (ggggaccactttgtacaagaaagctgggttAGAAGAATGAGGAAATGAGAGAGCATCCCA)
Alignment with template seq.1stアミノ酸配列92.60%一致2ndアミノ酸配列100%一致
Alignment with TAIR7CDSアミノ酸配列92.60%一致アミノ酸配列100%一致
comment1st:(Sequence解析まで行ったが、フレームがずれる)→2nd(09C10):100%
sequence 
>IE_07G09_1164bp
ATGATCACACCAATACACACACAACACTCCTTAATTCTCGTATATATAAACATTTATTCTCCACCTATTTTGTCAAAATTAAGAACAGGCTTCATTCTCT
GGACAAACACTCAAAAAACAAACAAAAAAAGGAACATGGAAGATCAGTTTCCTAAAATAGAAACTAGCTTCATGCACGACAAGCTCTTGTCTTCTGGAAT
CTACGGGTTCTTGAGTTCTTCGACGCCGCCACAACTTCTCGGTGTTCCAATATTTTTGGAAGGTATGAAATCTCCTCTTCTTCCTGCTTCTTCGACTCCG
AGCTACTTTGTGTCGCCTCATGATCATGAGCTCACATCTTCTATTCATCCATCTCCGGTAGCTTCTGTTCCTTGGAACTTTCTAGAATCTTTTCCTCAGT
CTCAACATCCTGATCATCATCCTTCTAAACCTCCAAACCTTACTTTGTTCCTTAAAGAACCAAAGCTACTAGAACTTTCTCAATCCGAAAGCAACATGAG
CCCTTACCATAAATACATCCCAAACTCCTTTTATCAATCAGACCAAAACAGAAACGAATGGGTAGAGATCAATAAAACTCTAACCAACTATCCCTCGAAA
GGTTTTGGAAACTATTGGCTAAGTACCACCAAGACTCAACCCATGAAGTCAAAAACAAGAAAGGTTGTTCAGACGACGACCCCAACAAAACTGTATAGAG
GAGTGAGACAAAGACACTGGGGCAAATGGGTCGCAGAGATTAGGCTTCCAAGGAACAGAACCCGTGTTTGGCTCGGCACTTTTGAAACCGCTGAGCAAGC
AGCAATGGCTTACGATACAGCAGCTTATATCCTTCGTGGCGAATTCGCACACCTCAACTTTCCTGATCTTAAACACCAGCTCAAGTCCGGTTCTTTGCGA
TGCATGATCGCCTCACTTTTGGAGTCCAAGATTCAACAGATCTCATCTTCCCAAGTAAGTAACTCTCCTTCTCCTCCTCCTCCAAAAGTGGGAACACCGG
AGCAAAAGAATCATCACATGAAGATGGAGTCAGGAGAAGACGTGATGATGAAGAAACAGAAAAGCCATAAGGAAGTGATGGAAGGAGATGGTGTACAATT
GAGTAGGATGCCTTCTTTGGATATGGATCTCATTTGGGATGCTCTCTCATTTCCTCATTCTTCT

>IE_07G09_translated
MITPIHTQHSLILVYINIYSPPILSKLRTGFILWTNTQKTNKKRNMEDQFPKIETSFMHDKLLSSGIYGFLSSSTPPQLLGVPIFLEGMKSPLLPASSTP
SYFVSPHDHELTSSIHPSPVASVPWNFLESFPQSQHPDHHPSKPPNLTLFLKEPKLLELSQSESNMSPYHKYIPNSFYQSDQNRNEWVEINKTLTNYPSK
GFGNYWLSTTKTQPMKSKTRKVVQTTTPTKLYRGVRQRHWGKWVAEIRLPRNRTRVWLGTFETAEQAAMAYDTAAYILRGEFAHLNFPDLKHQLKSGSLR
CMIASLLESKIQQISSSQVSNSPSPPPPKVGTPEQKNHHMKMESGEDVMMKKQKSHKEVMEGDGVQLSRMPSLDMDLIWDALSFPHSS
Alignment ◆CDS（TAIRver.9）_without_stop_codonとのalignment◆

100%一致しました。

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