◆◆◆IE_07G12 detail◆◆◆

Code nameIE_07G12
LocusAT1G51120
TemplateIT_07G12 (CR559)
Donor VectorpDONR207
Forward primerIPF_07G12 (ggggacaagtttgtacaaaaaagcaggcttcATGGACGAGATGAGCAATGTAGCCAAG)
Reverse primerIPR_07G12 (ggggaccactttgtacaagaaagctgggttTTGGATCCTAACACCAAACAGCATAAACCC)
Alignment with template seq.1stアミノ酸配列100%一致2nd-
Alignment with TAIR7CDSアミノ酸配列100%一致-
comment
sequence 
>IE_07G12_1056bp
ATGGACGAGATGAGCAATGTAGCCAAGACAACGACAGAGACTTCAGGCTTAACTGACTCTGTCTTGAGCCTCACGAAACGCATGAAACCTACTGAGGTTA
CGACCACCACAAAACCTGCCTTGTCCAACACGACGAAATTCAAAGGAGTTGTTCAGCAACAGAACGGTCATTGGGGTGCTCAGATTTACGCAGACCATCG
AAGGATTTGGCTTGGAACTTTCAAATCCGCTCATGAAGCCGCTGCTGCTTACGATAGCGCATCGATTAAGCTTCGAAGCTTTGATGCTAACTCGCACCGG
AACTTCCCTTGGTCTGATTTTACCCTCCATGAACCGGACTTTCAAGAGTGCTACACGACAGAAGCTGTGTTGAACATGATCAGAGACGGTTCTTATCAAC
ACAAGTTCAGAGATTTTCTCAGAATCCGGTCTCAGATTGTTGCGAATATCAACATCGTGGGATCAAAACAAGTCTTAGGAGGAGGAGAAGGTGGTCAAGA
ATCGAACAAGTGTTTCTCGTGCACGCAGCTTTTTCAGAAAGAACTGACACCGAGCGATGTAGGGAAACTGAATAGGCTTGTGATACCTAAGAAGTATGCA
GTGAAGTATATGCCTTTCATAAGCGATGATCAAAGCGAGAAAGAGACGAGTGAAGGAGTAGAAGATGTGGAGGTTGTCTTTTACGACAGAGCAATGAGAC
AATGGAAGTTTAGGTATTGTTACTGGAGAAGTAGCCAGAGCTTTGTCTTCACCAGAGGATGGAATGGTTTCGTGAAGGAGAAGAATCTCAAGGAGAAAGA
TATTATTGTCTTTTACACTTGCGATGTCCCCAACAATGTGAAGACATTAGAAGGCCAAAGCAAGACCTTCTTGATGATTGATGTTCATCACTTTTCAGGC
AACGGTTTCGTGGTTCCCGAGGAAGTAAACAAGACGGTTCATGAGATTTCTGATGAAGAGATGAAAACAGAAACCCTCTTTACCTCGAAGGTAGAAGAAG
AAACCAAATCAGAGGAGAAGAAAGGAGGGTTTATGCTGTTTGGTGTTAGGATCCAA

>IE_07G12_translated
MDEMSNVAKTTTETSGLTDSVLSLTKRMKPTEVTTTTKPALSNTTKFKGVVQQQNGHWGAQIYADHRRIWLGTFKSAHEAAAAYDSASIKLRSFDANSHR
NFPWSDFTLHEPDFQECYTTEAVLNMIRDGSYQHKFRDFLRIRSQIVANINIVGSKQVLGGGEGGQESNKCFSCTQLFQKELTPSDVGKLNRLVIPKKYA
VKYMPFISDDQSEKETSEGVEDVEVVFYDRAMRQWKFRYCYWRSSQSFVFTRGWNGFVKEKNLKEKDIIVFYTCDVPNNVKTLEGQSKTFLMIDVHHFSG
NGFVVPEEVNKTVHEISDEEMKTETLFTSKVEEETKSEEKKGGFMLFGVRIQ
Alignment ◆CDS（TAIRver.9）_without_stop_codonとのalignment◆

100%一致しました。

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