◆◆◆IE_07H01（IE_09C11） detail◆◆◆

Code nameIE_07H01（IE_09C11）
LocusAT1G51190
TemplateIT_07H01 (CR560)
Donor VectorpDONR207
Forward primerIPF_07H01 (ggggacaagtttgtacaaaaaagcaggcttcATGAATTCTAACAACTGGCTCGCGTTC)
Reverse primerIPR_07H01 (ggggaccactttgtacaagaaagctgggttTTCATTCCACATCGTGAAAACACCTCCTGG)
Alignment with template seq.1stアミノ酸配列99.80%一致2ndアミノ酸配列99.80%一致
Alignment with TAIR7CDSアミノ酸配列99.80%一致アミノ酸配列99.80%一致
comment1st:(Sequence解析まで行ったが、フレームはずれないが塩基置換がある)→2nd(09C11):70%, nt.63C→T, nt.238A→C, nt.423T→C, nt.1332C→T
sequence 
>IE_07H01_1704bp
ATGAATTCTAACAACTGGCTCGCGTTCCCTCTATCACCAACTCACTCTTCTTTGCCGCCTCATATTCACTCTTCACAAAATTCTCATTTCAATCTAGGTT
TGGTCAACGACAATATCGACAACCCTTTTCAAAACCAAGGATGGAATATGATCAATCCACATGGTGGAGGCGGCGAAGGTGGAGAGGTTCCAAAAGTGGC
TGATTTCTTAGGAGTGAGCAAATCGGGGGATCATCACCCCGATCACAACCTCGTACCTTATAACGACATTCATCAAACCAACGCCTCCGACTACTACTTT
CAAACCAATAGCTTGTTACCTACAGTCGTCACTTGTGCCTCTAATGCTCCTAATAATTATGAGCTTCAAGAGAGTGCACACAATTTGCAATCTCTCACTC
TCTCTATGGGAAGTACTGGAGCCGCCGCTGCAGAAGTCGCCACTGTGAAAGCCTCGCCGGCTGAGACTAGTGCCGATAATAGTAGCAGCACTACCAACAC
AAGTGGAGGAGCCATCGTTGAGGCTACACCGAGACGGACTTTGGAAACTTTTGGACAACGAACCTCTATCTATCGTGGAGTTACAAGACATAGATGGACC
GGTAGATATGAAGCTCATCTTTGGGATAATAGCTGTAGAAGAGAAGGACAATCAAGGAAAGGAAGACAAGTCTACTTAGGTGGGTATGACAAAGAAGAGA
AAGCAGCCAGAGCATATGATCTAGCTGCACTTAAATATTGGGGTCCCTCTACTACTACCAACTTTCCGATAACTAACTACGAGAAGGAAGTAGAGGAGAT
GAAAAACATGACGAGACAAGAGTTTGTGGCTTCTATAAGAAGGAAAAGTAGCGGATTCTCGCGTGGTGCATCCATGTATCGTGGAGTAACAAGGCATCAT
CAACATGGAAGATGGCAAGCAAGGATCGGCCGAGTTGCTGGAAACAAAGATCTCTACTTGGGAACATTCAGCACGGAGGAAGAAGCAGCAGAAGCTTATG
ACATAGCTGCGATAAAGTTTCGAGGTCTAAACGCGGTTACAAACTTTGAGATAAATCGGTATGATGTGAAAGCCATCCTGGAGAGCAACACACTTCCTAT
AGGAGGTGGTGCGGCTAAACGGCTCAAAGAAGCTCAAGCTCTAGAATCATCAAGAAAACGAGAGGAAATGATAGCCCTCGGATCAAATTTCCATCAATAT
GGTGCAGCGAGCGGCTCGAGCTCTGTTGCTTCCAGCTCTAGGCTTCAGCTTCAACCTTACCCTCTAAGCATTCAACAACCTTTTGAGCATCTTCATCATC
ATCAGCCTTTACTTACTCTACAGAACAACAATGATATCTCTCAGTATCATGATTCCTTTAGTTACATTCAGACGCAGCTTCATCTTCACCAACAACAAAC
CAACAATTACTTGCAGTCTTCTAGTCACACTTCACAGCTCTACAATGCTTATCTTCAGAGTAACCCTGGTCTGCTTCATGGATTTGTCTCTGATAATAAC
AACACTTCAGGGTTTCTTGGAAACAATGGGATTGGTATTGGGTCAAGCTCTACCGTTGGATCATCGGCTGAGGAAGAGTTTCCAGCCGTGAAAGTCGATT
ACGATATGCCTCCTTCCGGTGGAGCTACAGGGTATGGAGGATGGAATAGTGGAGAGTCTGCTCAAGGATCGAATCCAGGAGGTGTTTTCACGATGTGGAA
TGAA

>IE_07H01_translated
MNSNNWLAFPLSPTHSSLPPHIHSSQNSHFNLGLVNDNIDNPFQNQGWNMINPHGGGGEGGEVPKVADFLGVSKSGDHHPDHNLVPYNDIHQTNASDYYF
QTNSLLPTVVTCASNAPNNYELQESAHNLQSLTLSMGSTGAAAAEVATVKASPAETSADNSSSTTNTSGGAIVEATPRRTLETFGQRTSIYRGVTRHRWT
GRYEAHLWDNSCRREGQSRKGRQVYLGGYDKEEKAARAYDLAALKYWGPSTTTNFPITNYEKEVEEMKNMTRQEFVASIRRKSSGFSRGASMYRGVTRHH
QHGRWQARIGRVAGNKDLYLGTFSTEEEAAEAYDIAAIKFRGLNAVTNFEINRYDVKAILESNTLPIGGGAAKRLKEAQALESSRKREEMIALGSNFHQY
GAASGSSSVASSSRLQLQPYPLSIQQPFEHLHHHQPLLTLQNNNDISQYHDSFSYIQTQLHLHQQQTNNYLQSSSHTSQLYNAYLQSNPGLLHGFVSDNN
NTSGFLGNNGIGIGSSSTVGSSAEEEFPAVKVDYDMPPSGGATGYGGWNSGESAQGSNPGGVFTMWNE
Alignment ◆CDS（TAIRver.9）_without_stop_codonとのalignment◆

1700/1704 (99.8%)（アミノ酸配列で567/568 (99.8%)）一致しました。


               10        20        30        40        50        60        70        80        90       100
AT1G51 ATGAATTCTAACAACTGGCTCGCGTTCCCTCTATCACCAACTCACTCTTCTTTGCCGCCTCACATTCACTCTTCACAAAATTCTCATTTCAATCTAGGTT
       :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: :::::::::::::::::::::::::::::::::::::
IE_07H ATGAATTCTAACAACTGGCTCGCGTTCCCTCTATCACCAACTCACTCTTCTTTGCCGCCTCATATTCACTCTTCACAAAATTCTCATTTCAATCTAGGTT
               10        20        30        40        50        60        70        80        90       100

              110       120       130       140       150       160       170       180       190       200
AT1G51 TGGTCAACGACAATATCGACAACCCTTTTCAAAACCAAGGATGGAATATGATCAATCCACATGGTGGAGGCGGCGAAGGTGGAGAGGTTCCAAAAGTGGC
       ::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_07H TGGTCAACGACAATATCGACAACCCTTTTCAAAACCAAGGATGGAATATGATCAATCCACATGGTGGAGGCGGCGAAGGTGGAGAGGTTCCAAAAGTGGC
              110       120       130       140       150       160       170       180       190       200

              210       220       230       240       250       260       270       280       290       300
AT1G51 TGATTTCTTAGGAGTGAGCAAATCGGGGGATCATCACACCGATCACAACCTCGTACCTTATAACGACATTCATCAAACCAACGCCTCCGACTACTACTTT
       ::::::::::::::::::::::::::::::::::::: ::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_07H TGATTTCTTAGGAGTGAGCAAATCGGGGGATCATCACCCCGATCACAACCTCGTACCTTATAACGACATTCATCAAACCAACGCCTCCGACTACTACTTT
              210       220       230       240       250       260       270       280       290       300

              310       320       330       340       350       360       370       380       390       400
AT1G51 CAAACCAATAGCTTGTTACCTACAGTCGTCACTTGTGCCTCTAATGCTCCTAATAATTATGAGCTTCAAGAGAGTGCACACAATTTGCAATCTCTCACTC
       ::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_07H CAAACCAATAGCTTGTTACCTACAGTCGTCACTTGTGCCTCTAATGCTCCTAATAATTATGAGCTTCAAGAGAGTGCACACAATTTGCAATCTCTCACTC
              310       320       330       340       350       360       370       380       390       400

              410       420       430       440       450       460       470       480       490       500
AT1G51 TCTCTATGGGAAGTACTGGAGCTGCCGCTGCAGAAGTCGCCACTGTGAAAGCCTCGCCGGCTGAGACTAGTGCCGATAATAGTAGCAGCACTACCAACAC
       :::::::::::::::::::::: :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_07H TCTCTATGGGAAGTACTGGAGCCGCCGCTGCAGAAGTCGCCACTGTGAAAGCCTCGCCGGCTGAGACTAGTGCCGATAATAGTAGCAGCACTACCAACAC
              410       420       430       440       450       460       470       480       490       500

              510       520       530       540       550       560       570       580       590       600
AT1G51 AAGTGGAGGAGCCATCGTTGAGGCTACACCGAGACGGACTTTGGAAACTTTTGGACAACGAACCTCTATCTATCGTGGAGTTACAAGACATAGATGGACC
       ::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_07H AAGTGGAGGAGCCATCGTTGAGGCTACACCGAGACGGACTTTGGAAACTTTTGGACAACGAACCTCTATCTATCGTGGAGTTACAAGACATAGATGGACC
              510       520       530       540       550       560       570       580       590       600

              610       620       630       640       650       660       670       680       690       700
AT1G51 GGTAGATATGAAGCTCATCTTTGGGATAATAGCTGTAGAAGAGAAGGACAATCAAGGAAAGGAAGACAAGTCTACTTAGGTGGGTATGACAAAGAAGAGA
       ::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_07H GGTAGATATGAAGCTCATCTTTGGGATAATAGCTGTAGAAGAGAAGGACAATCAAGGAAAGGAAGACAAGTCTACTTAGGTGGGTATGACAAAGAAGAGA
              610       620       630       640       650       660       670       680       690       700

              710       720       730       740       750       760       770       780       790       800
AT1G51 AAGCAGCCAGAGCATATGATCTAGCTGCACTTAAATATTGGGGTCCCTCTACTACTACCAACTTTCCGATAACTAACTACGAGAAGGAAGTAGAGGAGAT
       ::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_07H AAGCAGCCAGAGCATATGATCTAGCTGCACTTAAATATTGGGGTCCCTCTACTACTACCAACTTTCCGATAACTAACTACGAGAAGGAAGTAGAGGAGAT
              710       720       730       740       750       760       770       780       790       800

              810       820       830       840       850       860       870       880       890       900
AT1G51 GAAAAACATGACGAGACAAGAGTTTGTGGCTTCTATAAGAAGGAAAAGTAGCGGATTCTCGCGTGGTGCATCCATGTATCGTGGAGTAACAAGGCATCAT
       ::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_07H GAAAAACATGACGAGACAAGAGTTTGTGGCTTCTATAAGAAGGAAAAGTAGCGGATTCTCGCGTGGTGCATCCATGTATCGTGGAGTAACAAGGCATCAT
              810       820       830       840       850       860       870       880       890       900

              910       920       930       940       950       960       970       980       990      1000
AT1G51 CAACATGGAAGATGGCAAGCAAGGATCGGCCGAGTTGCTGGAAACAAAGATCTCTACTTGGGAACATTCAGCACGGAGGAAGAAGCAGCAGAAGCTTATG
       ::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_07H CAACATGGAAGATGGCAAGCAAGGATCGGCCGAGTTGCTGGAAACAAAGATCTCTACTTGGGAACATTCAGCACGGAGGAAGAAGCAGCAGAAGCTTATG
              910       920       930       940       950       960       970       980       990      1000

             1010      1020      1030      1040      1050      1060      1070      1080      1090      1100
AT1G51 ACATAGCTGCGATAAAGTTTCGAGGTCTAAACGCGGTTACAAACTTTGAGATAAATCGGTATGATGTGAAAGCCATCCTGGAGAGCAACACACTTCCTAT
       ::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_07H ACATAGCTGCGATAAAGTTTCGAGGTCTAAACGCGGTTACAAACTTTGAGATAAATCGGTATGATGTGAAAGCCATCCTGGAGAGCAACACACTTCCTAT
             1010      1020      1030      1040      1050      1060      1070      1080      1090      1100

             1110      1120      1130      1140      1150      1160      1170      1180      1190      1200
AT1G51 AGGAGGTGGTGCGGCTAAACGGCTCAAAGAAGCTCAAGCTCTAGAATCATCAAGAAAACGAGAGGAAATGATAGCCCTCGGATCAAATTTCCATCAATAT
       ::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_07H AGGAGGTGGTGCGGCTAAACGGCTCAAAGAAGCTCAAGCTCTAGAATCATCAAGAAAACGAGAGGAAATGATAGCCCTCGGATCAAATTTCCATCAATAT
             1110      1120      1130      1140      1150      1160      1170      1180      1190      1200

             1210      1220      1230      1240      1250      1260      1270      1280      1290      1300
AT1G51 GGTGCAGCGAGCGGCTCGAGCTCTGTTGCTTCCAGCTCTAGGCTTCAGCTTCAACCTTACCCTCTAAGCATTCAACAACCTTTTGAGCATCTTCATCATC
       ::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_07H GGTGCAGCGAGCGGCTCGAGCTCTGTTGCTTCCAGCTCTAGGCTTCAGCTTCAACCTTACCCTCTAAGCATTCAACAACCTTTTGAGCATCTTCATCATC
             1210      1220      1230      1240      1250      1260      1270      1280      1290      1300

             1310      1320      1330      1340      1350      1360      1370      1380      1390      1400
AT1G51 ATCAGCCTTTACTTACTCTACAGAACAACAACGATATCTCTCAGTATCATGATTCCTTTAGTTACATTCAGACGCAGCTTCATCTTCACCAACAACAAAC
       ::::::::::::::::::::::::::::::: ::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_07H ATCAGCCTTTACTTACTCTACAGAACAACAATGATATCTCTCAGTATCATGATTCCTTTAGTTACATTCAGACGCAGCTTCATCTTCACCAACAACAAAC
             1310      1320      1330      1340      1350      1360      1370      1380      1390      1400

             1410      1420      1430      1440      1450      1460      1470      1480      1490      1500
AT1G51 CAACAATTACTTGCAGTCTTCTAGTCACACTTCACAGCTCTACAATGCTTATCTTCAGAGTAACCCTGGTCTGCTTCATGGATTTGTCTCTGATAATAAC
       ::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_07H CAACAATTACTTGCAGTCTTCTAGTCACACTTCACAGCTCTACAATGCTTATCTTCAGAGTAACCCTGGTCTGCTTCATGGATTTGTCTCTGATAATAAC
             1410      1420      1430      1440      1450      1460      1470      1480      1490      1500

             1510      1520      1530      1540      1550      1560      1570      1580      1590      1600
AT1G51 AACACTTCAGGGTTTCTTGGAAACAATGGGATTGGTATTGGGTCAAGCTCTACCGTTGGATCATCGGCTGAGGAAGAGTTTCCAGCCGTGAAAGTCGATT
       ::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_07H AACACTTCAGGGTTTCTTGGAAACAATGGGATTGGTATTGGGTCAAGCTCTACCGTTGGATCATCGGCTGAGGAAGAGTTTCCAGCCGTGAAAGTCGATT
             1510      1520      1530      1540      1550      1560      1570      1580      1590      1600

             1610      1620      1630      1640      1650      1660      1670      1680      1690      1700
AT1G51 ACGATATGCCTCCTTCCGGTGGAGCTACAGGGTATGGAGGATGGAATAGTGGAGAGTCTGCTCAAGGATCGAATCCAGGAGGTGTTTTCACGATGTGGAA
       ::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_07H ACGATATGCCTCCTTCCGGTGGAGCTACAGGGTATGGAGGATGGAATAGTGGAGAGTCTGCTCAAGGATCGAATCCAGGAGGTGTTTTCACGATGTGGAA
             1610      1620      1630      1640      1650      1660      1670      1680      1690      1700

           
AT1G51 TGAA
       ::::
IE_07H TGAA
           




               10        20        30        40        50        60        70        80        90       100
AT1G51 MNSNNWLAFPLSPTHSSLPPHIHSSQNSHFNLGLVNDNIDNPFQNQGWNMINPHGGGGEGGEVPKVADFLGVSKSGDHHTDHNLVPYNDIHQTNASDYYF
       ::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: ::::::::::::::::::::
IE_07H MNSNNWLAFPLSPTHSSLPPHIHSSQNSHFNLGLVNDNIDNPFQNQGWNMINPHGGGGEGGEVPKVADFLGVSKSGDHHPDHNLVPYNDIHQTNASDYYF
               10        20        30        40        50        60        70        80        90       100

              110       120       130       140       150       160       170       180       190       200
AT1G51 QTNSLLPTVVTCASNAPNNYELQESAHNLQSLTLSMGSTGAAAAEVATVKASPAETSADNSSSTTNTSGGAIVEATPRRTLETFGQRTSIYRGVTRHRWT
       ::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_07H QTNSLLPTVVTCASNAPNNYELQESAHNLQSLTLSMGSTGAAAAEVATVKASPAETSADNSSSTTNTSGGAIVEATPRRTLETFGQRTSIYRGVTRHRWT
              110       120       130       140       150       160       170       180       190       200

              210       220       230       240       250       260       270       280       290       300
AT1G51 GRYEAHLWDNSCRREGQSRKGRQVYLGGYDKEEKAARAYDLAALKYWGPSTTTNFPITNYEKEVEEMKNMTRQEFVASIRRKSSGFSRGASMYRGVTRHH
       ::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_07H GRYEAHLWDNSCRREGQSRKGRQVYLGGYDKEEKAARAYDLAALKYWGPSTTTNFPITNYEKEVEEMKNMTRQEFVASIRRKSSGFSRGASMYRGVTRHH
              210       220       230       240       250       260       270       280       290       300

              310       320       330       340       350       360       370       380       390       400
AT1G51 QHGRWQARIGRVAGNKDLYLGTFSTEEEAAEAYDIAAIKFRGLNAVTNFEINRYDVKAILESNTLPIGGGAAKRLKEAQALESSRKREEMIALGSNFHQY
       ::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_07H QHGRWQARIGRVAGNKDLYLGTFSTEEEAAEAYDIAAIKFRGLNAVTNFEINRYDVKAILESNTLPIGGGAAKRLKEAQALESSRKREEMIALGSNFHQY
              310       320       330       340       350       360       370       380       390       400

              410       420       430       440       450       460       470       480       490       500
AT1G51 GAASGSSSVASSSRLQLQPYPLSIQQPFEHLHHHQPLLTLQNNNDISQYHDSFSYIQTQLHLHQQQTNNYLQSSSHTSQLYNAYLQSNPGLLHGFVSDNN
       ::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_07H GAASGSSSVASSSRLQLQPYPLSIQQPFEHLHHHQPLLTLQNNNDISQYHDSFSYIQTQLHLHQQQTNNYLQSSSHTSQLYNAYLQSNPGLLHGFVSDNN
              410       420       430       440       450       460       470       480       490       500

              510       520       530       540       550       560        
AT1G51 NTSGFLGNNGIGIGSSSTVGSSAEEEFPAVKVDYDMPPSGGATGYGGWNSGESAQGSNPGGVFTMWNE
       ::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_07H NTSGFLGNNGIGIGSSSTVGSSAEEEFPAVKVDYDMPPSGGATGYGGWNSGESAQGSNPGGVFTMWNE
              510       520       530       540       550       560

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