Code name | IE_07H01ï¼IE_09C11ï¼ | |||
---|---|---|---|---|
Locus | AT1G51190 | |||
Template | IT_07H01 (CR560) | |||
Donor Vector | pDONR207 | |||
Forward primer | IPF_07H01 (ggggacaagtttgtacaaaaaagcaggcttcATGAATTCTAACAACTGGCTCGCGTTC) | |||
Reverse primer | IPR_07H01 (ggggaccactttgtacaagaaagctgggttTTCATTCCACATCGTGAAAACACCTCCTGG) | |||
Alignment with template seq. | 1st | ã¢ããé ¸é å99.80%ä¸è´ | 2nd | ã¢ããé ¸é å99.80%ä¸è´ |
Alignment with TAIR7CDS | ã¢ããé ¸é å99.80%ä¸è´ | ã¢ããé ¸é å99.80%ä¸è´ | ||
comment | 1st:(Sequence解æã¾ã§è¡ã£ããããã¬ã¼ã ã¯ãããªããå¡©åºç½®æããã)→2nd(09C11):70%, nt.63C→T, nt.238A→C, nt.423T→C, nt.1332C→T | |||
sequence |
>IE_07H01_1704bp ATGAATTCTAACAACTGGCTCGCGTTCCCTCTATCACCAACTCACTCTTCTTTGCCGCCTCATATTCACTCTTCACAAAATTCTCATTTCAATCTAGGTT TGGTCAACGACAATATCGACAACCCTTTTCAAAACCAAGGATGGAATATGATCAATCCACATGGTGGAGGCGGCGAAGGTGGAGAGGTTCCAAAAGTGGC TGATTTCTTAGGAGTGAGCAAATCGGGGGATCATCACCCCGATCACAACCTCGTACCTTATAACGACATTCATCAAACCAACGCCTCCGACTACTACTTT CAAACCAATAGCTTGTTACCTACAGTCGTCACTTGTGCCTCTAATGCTCCTAATAATTATGAGCTTCAAGAGAGTGCACACAATTTGCAATCTCTCACTC TCTCTATGGGAAGTACTGGAGCCGCCGCTGCAGAAGTCGCCACTGTGAAAGCCTCGCCGGCTGAGACTAGTGCCGATAATAGTAGCAGCACTACCAACAC AAGTGGAGGAGCCATCGTTGAGGCTACACCGAGACGGACTTTGGAAACTTTTGGACAACGAACCTCTATCTATCGTGGAGTTACAAGACATAGATGGACC GGTAGATATGAAGCTCATCTTTGGGATAATAGCTGTAGAAGAGAAGGACAATCAAGGAAAGGAAGACAAGTCTACTTAGGTGGGTATGACAAAGAAGAGA AAGCAGCCAGAGCATATGATCTAGCTGCACTTAAATATTGGGGTCCCTCTACTACTACCAACTTTCCGATAACTAACTACGAGAAGGAAGTAGAGGAGAT GAAAAACATGACGAGACAAGAGTTTGTGGCTTCTATAAGAAGGAAAAGTAGCGGATTCTCGCGTGGTGCATCCATGTATCGTGGAGTAACAAGGCATCAT CAACATGGAAGATGGCAAGCAAGGATCGGCCGAGTTGCTGGAAACAAAGATCTCTACTTGGGAACATTCAGCACGGAGGAAGAAGCAGCAGAAGCTTATG ACATAGCTGCGATAAAGTTTCGAGGTCTAAACGCGGTTACAAACTTTGAGATAAATCGGTATGATGTGAAAGCCATCCTGGAGAGCAACACACTTCCTAT AGGAGGTGGTGCGGCTAAACGGCTCAAAGAAGCTCAAGCTCTAGAATCATCAAGAAAACGAGAGGAAATGATAGCCCTCGGATCAAATTTCCATCAATAT GGTGCAGCGAGCGGCTCGAGCTCTGTTGCTTCCAGCTCTAGGCTTCAGCTTCAACCTTACCCTCTAAGCATTCAACAACCTTTTGAGCATCTTCATCATC ATCAGCCTTTACTTACTCTACAGAACAACAATGATATCTCTCAGTATCATGATTCCTTTAGTTACATTCAGACGCAGCTTCATCTTCACCAACAACAAAC CAACAATTACTTGCAGTCTTCTAGTCACACTTCACAGCTCTACAATGCTTATCTTCAGAGTAACCCTGGTCTGCTTCATGGATTTGTCTCTGATAATAAC AACACTTCAGGGTTTCTTGGAAACAATGGGATTGGTATTGGGTCAAGCTCTACCGTTGGATCATCGGCTGAGGAAGAGTTTCCAGCCGTGAAAGTCGATT ACGATATGCCTCCTTCCGGTGGAGCTACAGGGTATGGAGGATGGAATAGTGGAGAGTCTGCTCAAGGATCGAATCCAGGAGGTGTTTTCACGATGTGGAA TGAA >IE_07H01_translated MNSNNWLAFPLSPTHSSLPPHIHSSQNSHFNLGLVNDNIDNPFQNQGWNMINPHGGGGEGGEVPKVADFLGVSKSGDHHPDHNLVPYNDIHQTNASDYYF QTNSLLPTVVTCASNAPNNYELQESAHNLQSLTLSMGSTGAAAAEVATVKASPAETSADNSSSTTNTSGGAIVEATPRRTLETFGQRTSIYRGVTRHRWT GRYEAHLWDNSCRREGQSRKGRQVYLGGYDKEEKAARAYDLAALKYWGPSTTTNFPITNYEKEVEEMKNMTRQEFVASIRRKSSGFSRGASMYRGVTRHH QHGRWQARIGRVAGNKDLYLGTFSTEEEAAEAYDIAAIKFRGLNAVTNFEINRYDVKAILESNTLPIGGGAAKRLKEAQALESSRKREEMIALGSNFHQY GAASGSSSVASSSRLQLQPYPLSIQQPFEHLHHHQPLLTLQNNNDISQYHDSFSYIQTQLHLHQQQTNNYLQSSSHTSQLYNAYLQSNPGLLHGFVSDNN NTSGFLGNNGIGIGSSSTVGSSAEEEFPAVKVDYDMPPSGGATGYGGWNSGESAQGSNPGGVFTMWNE |
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Alignment |
◆CDS(TAIRver.9)_without_stop_codonとのalignment◆ 1700/1704 (99.8%)(アミノ酸配列で567/568 (99.8%))一致しました。 10 20 30 40 50 60 70 80 90 100 AT1G51 ATGAATTCTAACAACTGGCTCGCGTTCCCTCTATCACCAACTCACTCTTCTTTGCCGCCTCACATTCACTCTTCACAAAATTCTCATTTCAATCTAGGTT :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: ::::::::::::::::::::::::::::::::::::: IE_07H ATGAATTCTAACAACTGGCTCGCGTTCCCTCTATCACCAACTCACTCTTCTTTGCCGCCTCATATTCACTCTTCACAAAATTCTCATTTCAATCTAGGTT 10 20 30 40 50 60 70 80 90 100 110 120 130 140 150 160 170 180 190 200 AT1G51 TGGTCAACGACAATATCGACAACCCTTTTCAAAACCAAGGATGGAATATGATCAATCCACATGGTGGAGGCGGCGAAGGTGGAGAGGTTCCAAAAGTGGC :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_07H TGGTCAACGACAATATCGACAACCCTTTTCAAAACCAAGGATGGAATATGATCAATCCACATGGTGGAGGCGGCGAAGGTGGAGAGGTTCCAAAAGTGGC 110 120 130 140 150 160 170 180 190 200 210 220 230 240 250 260 270 280 290 300 AT1G51 TGATTTCTTAGGAGTGAGCAAATCGGGGGATCATCACACCGATCACAACCTCGTACCTTATAACGACATTCATCAAACCAACGCCTCCGACTACTACTTT ::::::::::::::::::::::::::::::::::::: :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_07H TGATTTCTTAGGAGTGAGCAAATCGGGGGATCATCACCCCGATCACAACCTCGTACCTTATAACGACATTCATCAAACCAACGCCTCCGACTACTACTTT 210 220 230 240 250 260 270 280 290 300 310 320 330 340 350 360 370 380 390 400 AT1G51 CAAACCAATAGCTTGTTACCTACAGTCGTCACTTGTGCCTCTAATGCTCCTAATAATTATGAGCTTCAAGAGAGTGCACACAATTTGCAATCTCTCACTC :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_07H CAAACCAATAGCTTGTTACCTACAGTCGTCACTTGTGCCTCTAATGCTCCTAATAATTATGAGCTTCAAGAGAGTGCACACAATTTGCAATCTCTCACTC 310 320 330 340 350 360 370 380 390 400 410 420 430 440 450 460 470 480 490 500 AT1G51 TCTCTATGGGAAGTACTGGAGCTGCCGCTGCAGAAGTCGCCACTGTGAAAGCCTCGCCGGCTGAGACTAGTGCCGATAATAGTAGCAGCACTACCAACAC :::::::::::::::::::::: ::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_07H TCTCTATGGGAAGTACTGGAGCCGCCGCTGCAGAAGTCGCCACTGTGAAAGCCTCGCCGGCTGAGACTAGTGCCGATAATAGTAGCAGCACTACCAACAC 410 420 430 440 450 460 470 480 490 500 510 520 530 540 550 560 570 580 590 600 AT1G51 AAGTGGAGGAGCCATCGTTGAGGCTACACCGAGACGGACTTTGGAAACTTTTGGACAACGAACCTCTATCTATCGTGGAGTTACAAGACATAGATGGACC :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_07H AAGTGGAGGAGCCATCGTTGAGGCTACACCGAGACGGACTTTGGAAACTTTTGGACAACGAACCTCTATCTATCGTGGAGTTACAAGACATAGATGGACC 510 520 530 540 550 560 570 580 590 600 610 620 630 640 650 660 670 680 690 700 AT1G51 GGTAGATATGAAGCTCATCTTTGGGATAATAGCTGTAGAAGAGAAGGACAATCAAGGAAAGGAAGACAAGTCTACTTAGGTGGGTATGACAAAGAAGAGA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_07H GGTAGATATGAAGCTCATCTTTGGGATAATAGCTGTAGAAGAGAAGGACAATCAAGGAAAGGAAGACAAGTCTACTTAGGTGGGTATGACAAAGAAGAGA 610 620 630 640 650 660 670 680 690 700 710 720 730 740 750 760 770 780 790 800 AT1G51 AAGCAGCCAGAGCATATGATCTAGCTGCACTTAAATATTGGGGTCCCTCTACTACTACCAACTTTCCGATAACTAACTACGAGAAGGAAGTAGAGGAGAT :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_07H AAGCAGCCAGAGCATATGATCTAGCTGCACTTAAATATTGGGGTCCCTCTACTACTACCAACTTTCCGATAACTAACTACGAGAAGGAAGTAGAGGAGAT 710 720 730 740 750 760 770 780 790 800 810 820 830 840 850 860 870 880 890 900 AT1G51 GAAAAACATGACGAGACAAGAGTTTGTGGCTTCTATAAGAAGGAAAAGTAGCGGATTCTCGCGTGGTGCATCCATGTATCGTGGAGTAACAAGGCATCAT :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_07H GAAAAACATGACGAGACAAGAGTTTGTGGCTTCTATAAGAAGGAAAAGTAGCGGATTCTCGCGTGGTGCATCCATGTATCGTGGAGTAACAAGGCATCAT 810 820 830 840 850 860 870 880 890 900 910 920 930 940 950 960 970 980 990 1000 AT1G51 CAACATGGAAGATGGCAAGCAAGGATCGGCCGAGTTGCTGGAAACAAAGATCTCTACTTGGGAACATTCAGCACGGAGGAAGAAGCAGCAGAAGCTTATG :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_07H CAACATGGAAGATGGCAAGCAAGGATCGGCCGAGTTGCTGGAAACAAAGATCTCTACTTGGGAACATTCAGCACGGAGGAAGAAGCAGCAGAAGCTTATG 910 920 930 940 950 960 970 980 990 1000 1010 1020 1030 1040 1050 1060 1070 1080 1090 1100 AT1G51 ACATAGCTGCGATAAAGTTTCGAGGTCTAAACGCGGTTACAAACTTTGAGATAAATCGGTATGATGTGAAAGCCATCCTGGAGAGCAACACACTTCCTAT :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_07H ACATAGCTGCGATAAAGTTTCGAGGTCTAAACGCGGTTACAAACTTTGAGATAAATCGGTATGATGTGAAAGCCATCCTGGAGAGCAACACACTTCCTAT 1010 1020 1030 1040 1050 1060 1070 1080 1090 1100 1110 1120 1130 1140 1150 1160 1170 1180 1190 1200 AT1G51 AGGAGGTGGTGCGGCTAAACGGCTCAAAGAAGCTCAAGCTCTAGAATCATCAAGAAAACGAGAGGAAATGATAGCCCTCGGATCAAATTTCCATCAATAT :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_07H AGGAGGTGGTGCGGCTAAACGGCTCAAAGAAGCTCAAGCTCTAGAATCATCAAGAAAACGAGAGGAAATGATAGCCCTCGGATCAAATTTCCATCAATAT 1110 1120 1130 1140 1150 1160 1170 1180 1190 1200 1210 1220 1230 1240 1250 1260 1270 1280 1290 1300 AT1G51 GGTGCAGCGAGCGGCTCGAGCTCTGTTGCTTCCAGCTCTAGGCTTCAGCTTCAACCTTACCCTCTAAGCATTCAACAACCTTTTGAGCATCTTCATCATC :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_07H GGTGCAGCGAGCGGCTCGAGCTCTGTTGCTTCCAGCTCTAGGCTTCAGCTTCAACCTTACCCTCTAAGCATTCAACAACCTTTTGAGCATCTTCATCATC 1210 1220 1230 1240 1250 1260 1270 1280 1290 1300 1310 1320 1330 1340 1350 1360 1370 1380 1390 1400 AT1G51 ATCAGCCTTTACTTACTCTACAGAACAACAACGATATCTCTCAGTATCATGATTCCTTTAGTTACATTCAGACGCAGCTTCATCTTCACCAACAACAAAC ::::::::::::::::::::::::::::::: :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_07H ATCAGCCTTTACTTACTCTACAGAACAACAATGATATCTCTCAGTATCATGATTCCTTTAGTTACATTCAGACGCAGCTTCATCTTCACCAACAACAAAC 1310 1320 1330 1340 1350 1360 1370 1380 1390 1400 1410 1420 1430 1440 1450 1460 1470 1480 1490 1500 AT1G51 CAACAATTACTTGCAGTCTTCTAGTCACACTTCACAGCTCTACAATGCTTATCTTCAGAGTAACCCTGGTCTGCTTCATGGATTTGTCTCTGATAATAAC :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_07H CAACAATTACTTGCAGTCTTCTAGTCACACTTCACAGCTCTACAATGCTTATCTTCAGAGTAACCCTGGTCTGCTTCATGGATTTGTCTCTGATAATAAC 1410 1420 1430 1440 1450 1460 1470 1480 1490 1500 1510 1520 1530 1540 1550 1560 1570 1580 1590 1600 AT1G51 AACACTTCAGGGTTTCTTGGAAACAATGGGATTGGTATTGGGTCAAGCTCTACCGTTGGATCATCGGCTGAGGAAGAGTTTCCAGCCGTGAAAGTCGATT :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_07H AACACTTCAGGGTTTCTTGGAAACAATGGGATTGGTATTGGGTCAAGCTCTACCGTTGGATCATCGGCTGAGGAAGAGTTTCCAGCCGTGAAAGTCGATT 1510 1520 1530 1540 1550 1560 1570 1580 1590 1600 1610 1620 1630 1640 1650 1660 1670 1680 1690 1700 AT1G51 ACGATATGCCTCCTTCCGGTGGAGCTACAGGGTATGGAGGATGGAATAGTGGAGAGTCTGCTCAAGGATCGAATCCAGGAGGTGTTTTCACGATGTGGAA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_07H ACGATATGCCTCCTTCCGGTGGAGCTACAGGGTATGGAGGATGGAATAGTGGAGAGTCTGCTCAAGGATCGAATCCAGGAGGTGTTTTCACGATGTGGAA 1610 1620 1630 1640 1650 1660 1670 1680 1690 1700 AT1G51 TGAA :::: IE_07H TGAA 10 20 30 40 50 60 70 80 90 100 AT1G51 MNSNNWLAFPLSPTHSSLPPHIHSSQNSHFNLGLVNDNIDNPFQNQGWNMINPHGGGGEGGEVPKVADFLGVSKSGDHHTDHNLVPYNDIHQTNASDYYF ::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: :::::::::::::::::::: IE_07H MNSNNWLAFPLSPTHSSLPPHIHSSQNSHFNLGLVNDNIDNPFQNQGWNMINPHGGGGEGGEVPKVADFLGVSKSGDHHPDHNLVPYNDIHQTNASDYYF 10 20 30 40 50 60 70 80 90 100 110 120 130 140 150 160 170 180 190 200 AT1G51 QTNSLLPTVVTCASNAPNNYELQESAHNLQSLTLSMGSTGAAAAEVATVKASPAETSADNSSSTTNTSGGAIVEATPRRTLETFGQRTSIYRGVTRHRWT :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_07H QTNSLLPTVVTCASNAPNNYELQESAHNLQSLTLSMGSTGAAAAEVATVKASPAETSADNSSSTTNTSGGAIVEATPRRTLETFGQRTSIYRGVTRHRWT 110 120 130 140 150 160 170 180 190 200 210 220 230 240 250 260 270 280 290 300 AT1G51 GRYEAHLWDNSCRREGQSRKGRQVYLGGYDKEEKAARAYDLAALKYWGPSTTTNFPITNYEKEVEEMKNMTRQEFVASIRRKSSGFSRGASMYRGVTRHH :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_07H GRYEAHLWDNSCRREGQSRKGRQVYLGGYDKEEKAARAYDLAALKYWGPSTTTNFPITNYEKEVEEMKNMTRQEFVASIRRKSSGFSRGASMYRGVTRHH 210 220 230 240 250 260 270 280 290 300 310 320 330 340 350 360 370 380 390 400 AT1G51 QHGRWQARIGRVAGNKDLYLGTFSTEEEAAEAYDIAAIKFRGLNAVTNFEINRYDVKAILESNTLPIGGGAAKRLKEAQALESSRKREEMIALGSNFHQY :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_07H QHGRWQARIGRVAGNKDLYLGTFSTEEEAAEAYDIAAIKFRGLNAVTNFEINRYDVKAILESNTLPIGGGAAKRLKEAQALESSRKREEMIALGSNFHQY 310 320 330 340 350 360 370 380 390 400 410 420 430 440 450 460 470 480 490 500 AT1G51 GAASGSSSVASSSRLQLQPYPLSIQQPFEHLHHHQPLLTLQNNNDISQYHDSFSYIQTQLHLHQQQTNNYLQSSSHTSQLYNAYLQSNPGLLHGFVSDNN :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_07H GAASGSSSVASSSRLQLQPYPLSIQQPFEHLHHHQPLLTLQNNNDISQYHDSFSYIQTQLHLHQQQTNNYLQSSSHTSQLYNAYLQSNPGLLHGFVSDNN 410 420 430 440 450 460 470 480 490 500 510 520 530 540 550 560 AT1G51 NTSGFLGNNGIGIGSSSTVGSSAEEEFPAVKVDYDMPPSGGATGYGGWNSGESAQGSNPGGVFTMWNE :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_07H NTSGFLGNNGIGIGSSSTVGSSAEEEFPAVKVDYDMPPSGGATGYGGWNSGESAQGSNPGGVFTMWNE 510 520 530 540 550 560 |