âââIE_07H02ï¼IE_09C12ï¼ detailâââ

Code name	IE_07H02ï¼IE_09C12ï¼
Locus	AT5G17430
Template	IT_07H02 (CR561)
Donor Vector	pDONR207
Forward primer	IPF_07H02 (ggggacaagtttgtacaaaaaagcaggcttcATGAACTCGATGAATAACTGGTTAGGC)
Reverse primer	IPR_07H02 (ggggaccactttgtacaagaaagctgggttAGTGTCGTTCCAAACTGAAAACGTTGGAGC)
Alignment with template seq.	1st	ã¢ããé¸éå99.10%ä¸è´	2nd	ã¢ããé¸éå99.10%ä¸è´
Alignment with TAIR7CDS	1st	ã¢ããé¸éå99.10%ä¸è´	2nd	ã¢ããé¸éå99.10%ä¸è´
comment	1st:(Sequenceè§£æã¾ã§è¡ã£ãããèªã¿ç´ãå¿è¦ããã?)→2nd(09C12):70%, nt.78C→T, 9nt_ins, nt.820T→A, nt.1029G→A, nt.1098C→T, nt.1185T→C, 3nt_del, nt.1449G→T, nt.1656G→A
sequence	>IE_07H02_1749bp ATGAACTCGATGAATAACTGGTTAGGCTTCTCTCTCTCTCCTCATGATCAAAATCATCACCGTACGGATGTTGACTCTTCCACCACCAGAACCGCCGTAG ATGTTGCCGGAGGGTACTGTTTTGATCTGGCCGCTCCCTCCGATGAATCTTCTGCCGTTCAAACATCTTTTCTTTCTCCTTTCGGTGTCACCCTCGAAGC TTTCACCAGAGACAATAATAGTCACTCCCGAGATTGGGACATCAATGGTGGTGCATGCAATAACATTAACAATAACGAACAAAATGGACCAAAGCTTGAG AATTTCCTCGGCCGCACCACCACGATTTACAATACCAACGAGACCGTTGTAGATGGAAATGGCGATTGTGGAGGAGGAGACGGTGGTGGTGGCGGCTCAC TAGGCCTTTCGATGATAAAAACATGGCTGAGTAATCATTCGGTTGCTAATGCTAATCATCAAGACAATGGTAACGGTGCACGAGGCTTGTCCCTCTCTAT GAATTCATCTACTAGTGATAGCAACAACTACAACAACAATGATGATGTCGTCCAAGAGAAGACTATTGTTGATGTCGTAGAAACTACACCGAAGAAAACT ATTGAGAGTTTTGGACAAAGGACGTCTATATACCGCGGTGTTACAAGGCATCGGTGGACAGGTAGATACGAGGCACATTTATGGGACAATAGTTGCAAAA GAGAAGGCCAGACTCGCAAAGGAAGACAAGTTTATCTGGGAGGTTATGACAAAGAAGAAAAAGCAGCTAGGGCTTACGATTTAGCCGCACTAAAGTATTG GGGAACCACCACTACTACTAACTTCCCCATGAGTGAATATGAGAAAGAGGTAGAAGAGATGAAGCACATGACGAGGCAAGAGTATGTTGCCTCTCTGCGC AGGAAAAGTAGTGGTTTCTCTCGTGGTGCATCGATTTATCGAGGAGTAACAAGGCATCACCAACATGGAAGGTGGCAAGCTAGGATCGGAAGAGTCGCCG GTAACAAAGACCTCTACTTGGGAACTTTCGGCACACAAGAAGAGGCTGCTGAGGCTTATGACATTGCAGCCATTAAATTCAGAGGATTAAGCGCAGTGAC TAACTTTGACATGAACAGATACAATGTTAAAGCAATCCTCGAGAGCCCGAGTCTACCTATTGGTAGTTCTGCGAAACGTCTCAAGGACGTTAACAATCCG GTTCCAGCTATGATGATTAGTAATAACGTTTCAGAGAGTGCAAATAATGTTAGCGGTTGGCAAAACACTGCGTTTCAGCATCAGGGAATGGATTTGAGCT TATTGCAGCAACAGCAGGAGAGGTACGTTGGTTATTACAATGGAGGAAACTTGTCTACCGAGAGTACTAGGGTTTGTTTCAAACAAGAGGAGGAACAACA ACACTTCTTGAGAAACTCGCCGAGTCACATGACTAATGTTGATCATCATAGCTCTACCTCTGATGATTCTGTTACCGTTTGTGGAAATGTTGTTAGTTAT GGTGGTTATCAAGGATTCGCAATCCCTGTTGGAACATCGGTTAATTACGATCCCTTTACTGCTGCTGAGATTGCTTACAACGCAAGAAATCATTATTACT ATGCTCAGCATCAGCAACAACAGCAGATTCAGCAGTCGCCGGGAGGAGATTTTCCGGTGGCAATTTCGAATAACCATAGCTCTAACATGTACTTTCACGG GGAAGGTGGTGGAGAAGGGGCTCCAACGTTTTCAGTTTGGAACGACACT >IE_07H02_translated MNSMNNWLGFSLSPHDQNHHRTDVDSSTTRTAVDVAGGYCFDLAAPSDESSAVQTSFLSPFGVTLEAFTRDNNSHSRDWDINGGACNNINNNEQNGPKLE NFLGRTTTIYNTNETVVDGNGDCGGGDGGGGGSLGLSMIKTWLSNHSVANANHQDNGNGARGLSLSMNSSTSDSNNYNNNDDVVQEKTIVDVVETTPKKT IESFGQRTSIYRGVTRHRWTGRYEAHLWDNSCKREGQTRKGRQVYLGGYDKEEKAARAYDLAALKYWGTTTTTNFPMSEYEKEVEEMKHMTRQEYVASLR RKSSGFSRGASIYRGVTRHHQHGRWQARIGRVAGNKDLYLGTFGTQEEAAEAYDIAAIKFRGLSAVTNFDMNRYNVKAILESPSLPIGSSAKRLKDVNNP VPAMMISNNVSESANNVSGWQNTAFQHQGMDLSLLQQQQERYVGYYNGGNLSTESTRVCFKQEEEQQHFLRNSPSHMTNVDHHSSTSDDSVTVCGNVVSY GGYQGFAIPVGTSVNYDPFTAAEIAYNARNHYYYAQHQQQQQIQQSPGGDFPVAISNNHSSNMYFHGEGGGEGAPTFSVWNDT
Alignment	◆CDS（TAIRver.9）_without_stop_codonとのalignment◆ 1742/1752 (99.4%)（アミノ酸配列で582/584 (99.7%)）一致しました。 10 20 30 40 50 60 70 80 90 100 AT5G17 ATGAACTCGATGAATAACTGGTTAGGCTTCTCTCTCTCTCCTCATGATCAAAATCATCACCGTACGGATGTTGACTCCTCCACCACCAGAACCGCCGTAG ::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: :::::::::::::::::::::: IE_07H ATGAACTCGATGAATAACTGGTTAGGCTTCTCTCTCTCTCCTCATGATCAAAATCATCACCGTACGGATGTTGACTCTTCCACCACCAGAACCGCCGTAG 10 20 30 40 50 60 70 80 90 100 110 120 130 140 150 160 170 180 190 200 AT5G17 ATGTTGCCGGAGGGTACTGTTTTGATCTGGCCGCTCCCTCCGATGAATCTTCTGCCGTTCAAACATCTTTTCTTTCTCCTTTCGGTGTCACCCTCGAAGC :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_07H ATGTTGCCGGAGGGTACTGTTTTGATCTGGCCGCTCCCTCCGATGAATCTTCTGCCGTTCAAACATCTTTTCTTTCTCCTTTCGGTGTCACCCTCGAAGC 110 120 130 140 150 160 170 180 190 200 210 220 230 240 250 260 270 280 290 300 AT5G17 TTTCACCAGAGACAATAATAGTCACTCCCGAGATTGGGACATCAATGGTGGTGCATGCAATAACATTAACAATAACGAACAAAATGGACCAAAGCTTGAG :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_07H TTTCACCAGAGACAATAATAGTCACTCCCGAGATTGGGACATCAATGGTGGTGCATGCAATAACATTAACAATAACGAACAAAATGGACCAAAGCTTGAG 210 220 230 240 250 260 270 280 290 300 310 320 330 340 350 360 370 380 390 400 AT5G17 AATTTCCTCGGCCGCACCACCACGATTTACAATACCAACGAGACCGTTGTAGATGGAAATGGCGATTGTGGAGGAGGAGACGGTGGTGGTGGCGGCTCAC :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_07H AATTTCCTCGGCCGCACCACCACGATTTACAATACCAACGAGACCGTTGTAGATGGAAATGGCGATTGTGGAGGAGGAGACGGTGGTGGTGGCGGCTCAC 310 320 330 340 350 360 370 380 390 400 410 420 430 440 450 460 470 480 490 500 AT5G17 TAGGCCTTTCGATGATAAAAACATGGCTGAGTAATCATTCGGTTGCTAATGCTAATCATCAAGACAATGGTAACGGTGCACGAGGCTTGTCCCTCTCTAT :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_07H TAGGCCTTTCGATGATAAAAACATGGCTGAGTAATCATTCGGTTGCTAATGCTAATCATCAAGACAATGGTAACGGTGCACGAGGCTTGTCCCTCTCTAT 410 420 430 440 450 460 470 480 490 500 510 520 530 540 550 560 570 580 590 600 AT5G17 GAATTCATCTACTAGTGATAGCAACAACTACAACAACAATGATGATGTCGTCCAAGAGAAGACTATTGTTGATGTCGTAGAAACTACACCGAAGAAAACT :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_07H GAATTCATCTACTAGTGATAGCAACAACTACAACAACAATGATGATGTCGTCCAAGAGAAGACTATTGTTGATGTCGTAGAAACTACACCGAAGAAAACT 510 520 530 540 550 560 570 580 590 600 610 620 630 640 650 660 670 680 690 700 AT5G17 ATTGAGAGTTTTGGACAAAGGACGTCTATATACCGCGGTGTTACAAGGCATCGGTGGACAGGTAGATACGAGGCACATTTATGGGACAATAGTTGCAAAA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_07H ATTGAGAGTTTTGGACAAAGGACGTCTATATACCGCGGTGTTACAAGGCATCGGTGGACAGGTAGATACGAGGCACATTTATGGGACAATAGTTGCAAAA 610 620 630 640 650 660 670 680 690 700 710 720 730 740 750 760 770 780 790 800 AT5G17 GAGAAGGCCAGACTCGCAAAGGAAGACAAGTTTATCTGGGAGGTTATGACAAAGAAGAAAAAGCAGCTAGGGCTTACGATTTAGCCGCACTAAAGTATTG :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_07H GAGAAGGCCAGACTCGCAAAGGAAGACAAGTTTATCTGGGAGGTTATGACAAAGAAGAAAAAGCAGCTAGGGCTTACGATTTAGCCGCACTAAAGTATTG 710 720 730 740 750 760 770 780 790 800 810 820 830 840 850 860 870 880 890 900 AT5G17 GGGAACCACCACTACTACTAACTTCCCCTTGAGTGAATATGAGAAAGAGGTAGAAGAGATGAAGCACATGACGAGGCAAGAGTATGTTGCCTCTCTGCGC :::::::::::::::::::::::::::: ::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_07H GGGAACCACCACTACTACTAACTTCCCCATGAGTGAATATGAGAAAGAGGTAGAAGAGATGAAGCACATGACGAGGCAAGAGTATGTTGCCTCTCTGCGC 810 820 830 840 850 860 870 880 890 900 910 920 930 940 950 960 970 980 990 1000 AT5G17 AGGAAAAGTAGTGGTTTCTCTCGTGGTGCATCGATTTATCGAGGAGTAACAAGGCATCACCAACATGGAAGGTGGCAAGCTAGGATCGGAAGAGTCGCCG :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_07H AGGAAAAGTAGTGGTTTCTCTCGTGGTGCATCGATTTATCGAGGAGTAACAAGGCATCACCAACATGGAAGGTGGCAAGCTAGGATCGGAAGAGTCGCCG 910 920 930 940 950 960 970 980 990 1000 1010 1020 1030 1040 1050 1060 1070 1080 1090 1100 AT5G17 GTAACAAAGACCTCTACTTGGGAACTTTCGGCACACAGGAAGAGGCTGCTGAGGCTTATGACATTGCAGCCATTAAATTCAGAGGATTAAGCGCAGTGAC ::::::::::::::::::::::::::::::::::::: :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_07H GTAACAAAGACCTCTACTTGGGAACTTTCGGCACACAAGAAGAGGCTGCTGAGGCTTATGACATTGCAGCCATTAAATTCAGAGGATTAAGCGCAGTGAC 1010 1020 1030 1040 1050 1060 1070 1080 1090 1100 1110 1120 1130 1140 1150 1160 1170 1180 1190 1200 AT5G17 TAACTTCGACATGAACAGATACAATGTTAAAGCAATCCTCGAGAGCCCGAGTCTACCTATTGGTAGTTCTGCGAAACGTCTCAAGGACGTTAATAATCCG :::::: :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: :::::: IE_07H TAACTTTGACATGAACAGATACAATGTTAAAGCAATCCTCGAGAGCCCGAGTCTACCTATTGGTAGTTCTGCGAAACGTCTCAAGGACGTTAACAATCCG 1110 1120 1130 1140 1150 1160 1170 1180 1190 1200 1210 1220 1230 1240 1250 1260 1270 1280 1290 1300 AT5G17 GTTCCAGCTATGATGATTAGTAATAACGTTTCAGAGAGTGCAAATAATGTTAGCGGTTGGCAAAACACTGCGTTTCAGCATCATCAGGGAATGGATTTGA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: ::::::::::::::::::: IE_07H GTTCCAGCTATGATGATTAGTAATAACGTTTCAGAGAGTGCAAATAATGTTAGCGGTTGGCAAAACACTGCGTTTCAG---CATCAGGGAATGGATTTGA 1210 1220 1230 1240 1250 1260 1270 1280 1290 1310 1320 1330 1340 1350 1360 1370 1380 1390 1400 AT5G17 GCTTATTGCAGCAACAGCAGGAGAGGTACGTTGGTTATTACAATGGAGGAAACTTGTCTACCGAGAGTACTAGGGTTTGTTTCAAACAAGAGGAGGAACA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_07H GCTTATTGCAGCAACAGCAGGAGAGGTACGTTGGTTATTACAATGGAGGAAACTTGTCTACCGAGAGTACTAGGGTTTGTTTCAAACAAGAGGAGGAACA 1300 1310 1320 1330 1340 1350 1360 1370 1380 1390 1410 1420 1430 1440 1450 1460 1470 1480 1490 1500 AT5G17 ACAACACTTCTTGAGAAACTCGCCGAGTCACATGACTAATGTTGATCATCATAGCTCGACCTCTGATGATTCTGTTACCGTTTGTGGAAATGTTGTTAGT ::::::::::::::::::::::::::::::::::::::::::::::::::::::::: :::::::::::::::::::::::::::::::::::::::::: IE_07H ACAACACTTCTTGAGAAACTCGCCGAGTCACATGACTAATGTTGATCATCATAGCTCTACCTCTGATGATTCTGTTACCGTTTGTGGAAATGTTGTTAGT 1400 1410 1420 1430 1440 1450 1460 1470 1480 1490 1510 1520 1530 1540 1550 1560 1570 1580 1590 1600 AT5G17 TATGGTGGTTATCAAGGATTCGCAATCCCTGTTGGAACATCGGTTAATTACGATCCCTTTACTGCTGCTGAGATTGCTTACAACGCAAGAAATCATTATT :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_07H TATGGTGGTTATCAAGGATTCGCAATCCCTGTTGGAACATCGGTTAATTACGATCCCTTTACTGCTGCTGAGATTGCTTACAACGCAAGAAATCATTATT 1500 1510 1520 1530 1540 1550 1560 1570 1580 1590 1610 1620 1630 1640 1650 1660 1670 1680 1690 1700 AT5G17 ACTATGCTCAGCATCAGCAACAACAGCAGATTCAGCAGTCGCCGGGAGGAGATTTTCCGGTGGCGATTTCGAATAACCATAGCTCTAACATGTACTTTCA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: ::::::::::::::::::::::::::::::::::: IE_07H ACTATGCTCAGCATCAGCAACAACAGCAGATTCAGCAGTCGCCGGGAGGAGATTTTCCGGTGGCAATTTCGAATAACCATAGCTCTAACATGTACTTTCA 1600 1610 1620 1630 1640 1650 1660 1670 1680 1690 1710 1720 1730 1740 1750 AT5G17 CGGGGAAGGTGGTGGAGAAGGGGCTCCAACGTTTTCAGTTTGGAACGACACT :::::::::::::::::::::::::::::::::::::::::::::::::::: IE_07H CGGGGAAGGTGGTGGAGAAGGGGCTCCAACGTTTTCAGTTTGGAACGACACT 1700 1710 1720 1730 1740 10 20 30 40 50 60 70 80 90 100 AT5G17 MNSMNNWLGFSLSPHDQNHHRTDVDSSTTRTAVDVAGGYCFDLAAPSDESSAVQTSFLSPFGVTLEAFTRDNNSHSRDWDINGGACNNINNNEQNGPKLE :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_07H MNSMNNWLGFSLSPHDQNHHRTDVDSSTTRTAVDVAGGYCFDLAAPSDESSAVQTSFLSPFGVTLEAFTRDNNSHSRDWDINGGACNNINNNEQNGPKLE 10 20 30 40 50 60 70 80 90 100 110 120 130 140 150 160 170 180 190 200 AT5G17 NFLGRTTTIYNTNETVVDGNGDCGGGDGGGGGSLGLSMIKTWLSNHSVANANHQDNGNGARGLSLSMNSSTSDSNNYNNNDDVVQEKTIVDVVETTPKKT :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_07H NFLGRTTTIYNTNETVVDGNGDCGGGDGGGGGSLGLSMIKTWLSNHSVANANHQDNGNGARGLSLSMNSSTSDSNNYNNNDDVVQEKTIVDVVETTPKKT 110 120 130 140 150 160 170 180 190 200 210 220 230 240 250 260 270 280 290 300 AT5G17 IESFGQRTSIYRGVTRHRWTGRYEAHLWDNSCKREGQTRKGRQVYLGGYDKEEKAARAYDLAALKYWGTTTTTNFPLSEYEKEVEEMKHMTRQEYVASLR ::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::.::::::::::::::::::::::: IE_07H IESFGQRTSIYRGVTRHRWTGRYEAHLWDNSCKREGQTRKGRQVYLGGYDKEEKAARAYDLAALKYWGTTTTTNFPMSEYEKEVEEMKHMTRQEYVASLR 210 220 230 240 250 260 270 280 290 300 310 320 330 340 350 360 370 380 390 400 AT5G17 RKSSGFSRGASIYRGVTRHHQHGRWQARIGRVAGNKDLYLGTFGTQEEAAEAYDIAAIKFRGLSAVTNFDMNRYNVKAILESPSLPIGSSAKRLKDVNNP :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_07H RKSSGFSRGASIYRGVTRHHQHGRWQARIGRVAGNKDLYLGTFGTQEEAAEAYDIAAIKFRGLSAVTNFDMNRYNVKAILESPSLPIGSSAKRLKDVNNP 310 320 330 340 350 360 370 380 390 400 410 420 430 440 450 460 470 480 490 500 AT5G17 VPAMMISNNVSESANNVSGWQNTAFQHHQGMDLSLLQQQQERYVGYYNGGNLSTESTRVCFKQEEEQQHFLRNSPSHMTNVDHHSSTSDDSVTVCGNVVS :::::::::::::::::::::::::: ::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_07H VPAMMISNNVSESANNVSGWQNTAFQ-HQGMDLSLLQQQQERYVGYYNGGNLSTESTRVCFKQEEEQQHFLRNSPSHMTNVDHHSSTSDDSVTVCGNVVS 410 420 430 440 450 460 470 480 490 510 520 530 540 550 560 570 580 AT5G17 YGGYQGFAIPVGTSVNYDPFTAAEIAYNARNHYYYAQHQQQQQIQQSPGGDFPVAISNNHSSNMYFHGEGGGEGAPTFSVWNDT :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_07H YGGYQGFAIPVGTSVNYDPFTAAEIAYNARNHYYYAQHQQQQQIQQSPGGDFPVAISNNHSSNMYFHGEGGGEGAPTFSVWNDT 500 510 520 530 540 550 560 570 580

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âââIE_07H02ï¼IE_09C12ï¼ detailâââ

âââIE_07H02ï¼IE_09C12ï¼ detailâââ