◆◆◆IE_07H08 detail◆◆◆

Code nameIE_07H08
LocusAT3G03200
TemplateIT_07H08 (CR639)
Donor VectorpDONR207
Forward primerIPF_07H08 (ggggacaagtttgtacaaaaaagcaggcttcATGGCTCCTGTCTCGTTACCTCCAGGT)
Reverse primerIPR_07H08 (ggggaccactttgtacaagaaagctgggttGAAAAAAATAGCAAGACATTTGTAGTATTG)
Alignment with template seq.1stアミノ酸配列96.70%一致2ndアミノ酸配列96.70%一致
Alignment with TAIR7CDSアミノ酸配列96.70%一致アミノ酸配列96.70%一致
comment1st:(Sequence解析まで行ったが、読み直す必要がある?), 2nd:70%, 24nt_ins, 24nt_del
sequence 
>IE_07H08_1437bp
ATGGCTCCTGTCTCGTTACCTCCAGGTTTCCGATTCCATCCAACAGACGAGGAACTAATTACTTACTATCTAAAAAGAAAGATCAACGGTCTAGAAATCG
AACTTGAAGTTATCGCTGAAGTTGATCTTTACAAGTGTGAGCCATGGGACTTACCAGGGAAGTCCTTGCTTCCGAGCAAAGACCAAGAATGGTACTTCTT
CAGCCCACGAGACCGGAAGTATCCCAACGGCTCAAGGACAAACCGGGCAACTAAAGGCGGTTATTGGAAGGCTACAGGTAAAGACCGCCGAGTTAGTTGG
AGAGACCGAGCCATAGGAACCAAGAAGACATTGGTTTACTACCGTGGGCGCGCGCCACATGGCATAAGAACTGGTTGGGTCATGCACGAATATCGACTTG
ATGAAACAGAATGTGAGCCTTCTGCATACGGCATGCAGGACGCATATGCACTTTGTCGTGTGTTCAAAAAGATTGTTATTGAAGCTAAGCCAAGAGATCA
ACATCGGTCATATGTCCACGCGATGTCGAATGTGAGTGGTAATTGCTCATCGAGTTTTGACACTTGTTCGGATCTCGAAATCAGTTCAACTACTCATCAA
GTTCAAAACACATTCCAACCGCGATTTGGCAACGAGCGATTTAACTCCAACGCAATCAGCAACGAGGATTGGTCACAATACTACGGTTCTTCTTATAGAC
CGTTCCCTACTCCATATAAGGTTAACACAGAGATCGAATGTTCAATGTTACAACACAATATATATCTACCACCGTTGCGTGTAGAGAACTCTGCGTTTAG
TGATTCCGATTTCTTCACGAGTATGACTCACAACAACGACCATGGCGTTTTCGATGACTTTACTTTTGCTGCAAGTAACTCCAACCACAATAATAGCGTT
GGTGATCAAGTGATCCACGTTGGCAATTATGATGAACAATTAATAACATCTAACCGTCATATGAACCAGACTGGTTATATAAAAGAGCAGAAGATCAGAT
CGAGTTTGGATAATACTGACGAAGATCCAGGATTTCATGGTAACAATACCAATGACAACATAGATATCGATGATTTTCTCTCGTTTGATATATATAACGA
GGACAACGTGAATCAAATAGAAGATAATGAAGACGTGAATACAAATGAAACCCTTGATTCATCGGGATTCGAGGTGGTTGAAGAAGAAACTAGATTTAAC
AACCAAATGCTCATCTCGACATATCAAACGACAAAGATTCTATATCACCAAGTCGTACCTTGTCACACGTTGAAAGTTCACGTCAATCCTATTAGTCACA
ATGTGGAAGAGAGAACATTGTTCATTGAAGAGGACAAAGATTCTTGGTTACAAAGAGCTGAGAAGATCACGAAGACAAAACTAACACTTTTTAGTTTAAT
GGCTCAGCAATACTACAAATGTCTTGCTATTTTTTTC

>IE_07H08_translated
MAPVSLPPGFRFHPTDEELITYYLKRKINGLEIELEVIAEVDLYKCEPWDLPGKSLLPSKDQEWYFFSPRDRKYPNGSRTNRATKGGYWKATGKDRRVSW
RDRAIGTKKTLVYYRGRAPHGIRTGWVMHEYRLDETECEPSAYGMQDAYALCRVFKKIVIEAKPRDQHRSYVHAMSNVSGNCSSSFDTCSDLEISSTTHQ
VQNTFQPRFGNERFNSNAISNEDWSQYYGSSYRPFPTPYKVNTEIECSMLQHNIYLPPLRVENSAFSDSDFFTSMTHNNDHGVFDDFTFAASNSNHNNSV
GDQVIHVGNYDEQLITSNRHMNQTGYIKEQKIRSSLDNTDEDPGFHGNNTNDNIDIDDFLSFDIYNEDNVNQIEDNEDVNTNETLDSSGFEVVEEETRFN
NQMLISTYQTTKILYHQVVPCHTLKVHVNPISHNVEERTLFIEEDKDSWLQRAEKITKTKLTLFSLMAQQYYKCLAIFF
Alignment ◆CDS（TAIRver.9）_without_stop_codonとのalignment◆

100%一致しました。

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