| Code name | IE_08A10 | |||
|---|---|---|---|---|
| Locus | AT5G67000 | |||
| Template | IT_08A10 (CR024) | |||
| Donor Vector | pDONR207 | |||
| Forward primer | IPF_08A10 (ggggacaagtttgtacaaaaaagcaggcttcATGGATAATTCAGAAAATGTT) | |||
| Reverse primer | IPR_08A10 (ggggaccactttgtacaagaaagctgggttTCTCCACCGCCGTTTAATTCCTTCTCCGTT) | |||
| Alignment with template seq. | 1st | アミノ酸配列100%一致 | 2nd | - |
| Alignment with TAIR7CDS | アミノ酸配列87.00%一致 | - | ||
| comment | 1st:Templateの配列とは一致 | |||
| sequence |
>IE_08A10_480bp ATGGATAATTCAGAAAATGTTCTATTTAATGATCAAAACGAAAATTTCACACTTGTTGCACCACACCCTTCTTCTTCGTACTTGACAAGAGATCAAGAGC ACGAGATCATGGTCTCTGCTCTGCGACAAGTGATATCTAACTCCGGAGCTGACGACGCGTCATCATCAAACTTGATCATCACAAGCGTTCCGCCTCCAGA CGCTGGCCCTTGTCCTCTCTGTGGCGTCGCCGGTTGCTACGGCTGCACATTACAACGGCCGCACCGAGAGGTAAAGAAGGAGAAGAAATACAAAGGAGTA AGGAAAAAACCATCGGGTAAATGGGCGGCGGAGATATGGGATCCGAGATCGAAATCAAGGAGGTGGCTTGGAACGTTTCTTACGGCGGAGATGGCGGCAC AATCTTACAATGATGCGGCGGCTGAGTATCGAGCAAGACGTGGTAAAACAAACGGAGAAGGAATTAAACGGCGGTGGAGA >IE_08A10_translated MDNSENVLFNDQNENFTLVAPHPSSSYLTRDQEHEIMVSALRQVISNSGADDASSSNLIITSVPPPDAGPCPLCGVAGCYGCTLQRPHREVKKEKKYKGV RKKPSGKWAAEIWDPRSKSRRWLGTFLTAEMAAQSYNDAAAEYRARRGKTNGEGIKRRWR |
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| Alignment |
◆CDS（TAIRver.9）_without_stop_codonとのalignment◆ 480/552 (87.0%)（アミノ酸配列で160/184 (87.0%)）一致しました。
10 20 30 40 50 60 70 80 90 100
AT5G67 ATGCTCACTCCCTTTTGTTCTTCACACCATTTGCAGGAAAAAATGAATAGTTGTCAGTCTAATCCCACCAAAATGGATAATTCAGAAAATGTTCTATTTA
::::::::::::::::::::::::::::
IE_08A ------------------------------------------------------------------------ATGGATAATTCAGAAAATGTTCTATTTA
10 20
110 120 130 140 150 160 170 180 190 200
AT5G67 ATGATCAAAACGAAAATTTCACACTTGTTGCACCACACCCTTCTTCTTCGTACTTGACAAGAGATCAAGAGCACGAGATCATGGTCTCTGCTCTGCGACA
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_08A ATGATCAAAACGAAAATTTCACACTTGTTGCACCACACCCTTCTTCTTCGTACTTGACAAGAGATCAAGAGCACGAGATCATGGTCTCTGCTCTGCGACA
30 40 50 60 70 80 90 100 110 120
210 220 230 240 250 260 270 280 290 300
AT5G67 AGTGATATCTAACTCCGGAGCTGACGACGCGTCATCATCAAACTTGATCATCACAAGCGTTCCGCCTCCAGACGCTGGCCCTTGTCCTCTCTGTGGCGTC
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_08A AGTGATATCTAACTCCGGAGCTGACGACGCGTCATCATCAAACTTGATCATCACAAGCGTTCCGCCTCCAGACGCTGGCCCTTGTCCTCTCTGTGGCGTC
130 140 150 160 170 180 190 200 210 220
310 320 330 340 350 360 370 380 390 400
AT5G67 GCCGGTTGCTACGGCTGCACATTACAACGGCCGCACCGAGAGGTAAAGAAGGAGAAGAAATACAAAGGAGTAAGGAAAAAACCATCGGGTAAATGGGCGG
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_08A GCCGGTTGCTACGGCTGCACATTACAACGGCCGCACCGAGAGGTAAAGAAGGAGAAGAAATACAAAGGAGTAAGGAAAAAACCATCGGGTAAATGGGCGG
230 240 250 260 270 280 290 300 310 320
410 420 430 440 450 460 470 480 490 500
AT5G67 CGGAGATATGGGATCCGAGATCGAAATCAAGGAGGTGGCTTGGAACGTTTCTTACGGCGGAGATGGCGGCACAATCTTACAATGATGCGGCGGCTGAGTA
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_08A CGGAGATATGGGATCCGAGATCGAAATCAAGGAGGTGGCTTGGAACGTTTCTTACGGCGGAGATGGCGGCACAATCTTACAATGATGCGGCGGCTGAGTA
330 340 350 360 370 380 390 400 410 420
510 520 530 540 550
AT5G67 TCGAGCAAGACGTGGTAAAACAAACGGAGAAGGAATTAAACGGCGGTGGAGA
::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_08A TCGAGCAAGACGTGGTAAAACAAACGGAGAAGGAATTAAACGGCGGTGGAGA
430 440 450 460 470 480
10 20 30 40 50 60 70 80 90 100
AT5G67 MLTPFCSSHHLQEKMNSCQSNPTKMDNSENVLFNDQNENFTLVAPHPSSSYLTRDQEHEIMVSALRQVISNSGADDASSSNLIITSVPPPDAGPCPLCGV
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_08A ------------------------MDNSENVLFNDQNENFTLVAPHPSSSYLTRDQEHEIMVSALRQVISNSGADDASSSNLIITSVPPPDAGPCPLCGV
10 20 30 40 50 60 70
110 120 130 140 150 160 170 180
AT5G67 AGCYGCTLQRPHREVKKEKKYKGVRKKPSGKWAAEIWDPRSKSRRWLGTFLTAEMAAQSYNDAAAEYRARRGKTNGEGIKRRWR
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_08A AGCYGCTLQRPHREVKKEKKYKGVRKKPSGKWAAEIWDPRSKSRRWLGTFLTAEMAAQSYNDAAAEYRARRGKTNGEGIKRRWR
80 90 100 110 120 130 140 150 160
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