| Code name | IE_08B06 | |||
|---|---|---|---|---|
| Locus | AT1G68800 | |||
| Template | IT_08B06 (CR118) | |||
| Donor Vector | pDONR207 | |||
| Forward primer | IPF_08B06 (ggggacaagtttgtacaaaaaagcaggcttcATGTTTCCTTCTTTCATTACTCACATT) | |||
| Reverse primer | IPR_08B06 (ggggaccactttgtacaagaaagctgggttATTAGGGTTTTTAGTTAACACATTGCTGTT) | |||
| Alignment with template seq. | 1st | アミノ酸配列100%一致 | 2nd | - |
| Alignment with TAIR7CDS | アミノ酸配列100%一致 | - | ||
| comment | ||||
| sequence |
>IE_08B06_951bp ATGTTTCCTTCTTTCATTACTCACATTCAAAGCCCTAATTCTCACCATCACTACTCTTCGCCTTCTTTTCCTTTCTCTTCCGATTTTCTTGAGAGTTTTG ATGAATCCTTCTTGATAAACCAATTCTTGTTACAGCAGCAAGATGTAGCAGCAAATGTTGTTGAATCTCCTTGGAAATTTTGCAAGAAGCTTGAGCTTAA GAAGAAGAATGAGAAGTGTGTTGATGGAAGCACCTCACAAGAGGTTCAATGGAGAAGGACGGTCAAAAAAAGGGACAGGCATAGTAAGATCTGCACGGCT CAAGGTCCTAGAGACCGGAGGATGAGGCTGTCTCTTCAGATTGCTCGCAAGTTTTTCGATCTTCAAGACATGTTGGGTTTCGACAAGGCGAGCAAGACGA TTGAATGGCTTTTCTCCAAATCAAAGACTTCCATCAAACAACTTAAAGAAAGAGTGGCTGCATCGGAAGGAGGAGGAAAGGATGAACATCTCCAGGTTGA TGAAAAGGAAAAGGATGAGACACTGAAGTTGAGAGTCTCAAAGAGAAGAACAAAGACTATGGAGAGCTCTTTTAAGACTAAAGAGTCGAGAGAGAGAGCT AGAAAGCGAGCAAGAGAGAGAACAATGGCAAAGATGAAGATGAGATTATTTGAGACCTCGGAAACAATTTCAGATCCTCATCAAGAAACTAGAGAGATCA AGATAACCAATGGTGTACAATTACTAGAAAAGGAAAATAAAGAACAAGAATGGAGTAATACTAATGATGTTCACATGGTAGAGTATCAAATGGATTCTGT GAGCATCATAGAGAAGTTTCTTGGACTAACCAGTGACTCTAGCTCCTCTTCCATTTTTGGTGACTCCGAGGAATGTTACACAAGTCTTAGTTCAGTAAGA GGTACAATTTCAGCAGCAGGTAACAGCAATGTGTTAACTAAAAACCCTAAT >IE_08B06_translated MFPSFITHIQSPNSHHHYSSPSFPFSSDFLESFDESFLINQFLLQQQDVAANVVESPWKFCKKLELKKKNEKCVDGSTSQEVQWRRTVKKRDRHSKICTA QGPRDRRMRLSLQIARKFFDLQDMLGFDKASKTIEWLFSKSKTSIKQLKERVAASEGGGKDEHLQVDEKEKDETLKLRVSKRRTKTMESSFKTKESRERA RKRARERTMAKMKMRLFETSETISDPHQETREIKITNGVQLLEKENKEQEWSNTNDVHMVEYQMDSVSIIEKFLGLTSDSSSSSIFGDSEECYTSLSSVR GTISAAGNSNVLTKNPN |
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| Alignment |
◆CDS（TAIRver.9）_without_stop_codonとのalignment◆ 936/1073 (87.2%)（アミノ酸配列で305/356 (85.7%)）一致しました。
10 20 30 40 50 60 70 80 90 100
AT1G68 ATGTTTCCTTCTCTAGATACCAATGGCTATGACCTCTTTGATCCCTTCATTCCCCATCAAACAACCATGTTTCCTTCTTTCATTACTCACATTCAAAGCC
::::::::::::::::::::::::::::::::::
IE_08B ------------------------------------------------------------------ATGTTTCCTTCTTTCATTACTCACATTCAAAGCC
10 20 30
110 120 130 140 150 160 170 180 190 200
AT1G68 CTAATTCTCACCATCACTACTCTTCGCCTTCTTTTCCTTTCTCTTCCGATTTTCTTGAGAGTTTTGATGAATCCTTCTTGATAAACCAATTCTTGTTACA
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_08B CTAATTCTCACCATCACTACTCTTCGCCTTCTTTTCCTTTCTCTTCCGATTTTCTTGAGAGTTTTGATGAATCCTTCTTGATAAACCAATTCTTGTTACA
40 50 60 70 80 90 100 110 120 130
210 220 230 240 250 260 270 280 290 300
AT1G68 GCAGCAAGATGTAGCAGCAAATGTTGTTGAATCTCCTTGGAAATTTTGCAAGAAGCTTGAGCTTAAGAAGAAGAATGAGAAGTGTGTTGATGGAAGCACC
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_08B GCAGCAAGATGTAGCAGCAAATGTTGTTGAATCTCCTTGGAAATTTTGCAAGAAGCTTGAGCTTAAGAAGAAGAATGAGAAGTGTGTTGATGGAAGCACC
140 150 160 170 180 190 200 210 220 230
310 320 330 340 350 360 370 380 390 400
AT1G68 TCACAAGAGGTTCAATGGAGAAGGACGGTCAAAAAAAGGGACAGGCATAGTAAGATCTGCACGGCTCAAGGTCCTAGAGACCGGAGGATGAGGCTGTCTC
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_08B TCACAAGAGGTTCAATGGAGAAGGACGGTCAAAAAAAGGGACAGGCATAGTAAGATCTGCACGGCTCAAGGTCCTAGAGACCGGAGGATGAGGCTGTCTC
240 250 260 270 280 290 300 310 320 330
410 420 430 440 450 460 470 480 490 500
AT1G68 TTCAGATTGCTCGCAAGTTTTTCGATCTTCAAGACATGTTGGGTTTCGACAAGGCGAGCAAGACGATTGAATGGCTTTTCTCCAAATCAAAGACTTCCAT
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_08B TTCAGATTGCTCGCAAGTTTTTCGATCTTCAAGACATGTTGGGTTTCGACAAGGCGAGCAAGACGATTGAATGGCTTTTCTCCAAATCAAAGACTTCCAT
340 350 360 370 380 390 400 410 420 430
510 520 530 540 550 560 570 580 590 600
AT1G68 CAAACAACTTAAAGAAAGAGTGGCTGCATCGGAAGGAGGAGGAAAGGATGAACATCTCCAGGTTGATGAAAAGGAAAAGGATGAGACACTGAAGTTGAGA
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_08B CAAACAACTTAAAGAAAGAGTGGCTGCATCGGAAGGAGGAGGAAAGGATGAACATCTCCAGGTTGATGAAAAGGAAAAGGATGAGACACTGAAGTTGAGA
440 450 460 470 480 490 500 510 520 530
610 620 630 640 650 660 670 680 690 700
AT1G68 GTCTCAAAGAGAAGAACAAAGACTATGGAGAGCTCTTTTAAGACTAAAGAGTCGAGAGAGAGAGCTAGAAAGCGAGCAAGAGAGAGAACAATGGCAAAGA
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_08B GTCTCAAAGAGAAGAACAAAGACTATGGAGAGCTCTTTTAAGACTAAAGAGTCGAGAGAGAGAGCTAGAAAGCGAGCAAGAGAGAGAACAATGGCAAAGA
540 550 560 570 580 590 600 610 620 630
710 720 730 740 750 760 770 780 790 800
AT1G68 TGAAGATGAGATTATTTGAGACCTCGGAAACAATTTCAGATCCTCATCAAGAAACTAGAGAGATCAAGATAACCAATGGTGTACAATTACTAGAAAAGGA
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_08B TGAAGATGAGATTATTTGAGACCTCGGAAACAATTTCAGATCCTCATCAAGAAACTAGAGAGATCAAGATAACCAATGGTGTACAATTACTAGAAAAGGA
640 650 660 670 680 690 700 710 720 730
810 820 830 840 850 860 870 880 890 900
AT1G68 AAATAAAGAACAAGAATGGAGTAATACTAATGATGTTCACATGGTAGAGTATCAAATGGATTCTGTGAGCATCATAGAGAAGTTTCTTGGACTAACCAGT
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_08B AAATAAAGAACAAGAATGGAGTAATACTAATGATGTTCACATGGTAGAGTATCAAATGGATTCTGTGAGCATCATAGAGAAGTTTCTTGGACTAACCAGT
740 750 760 770 780 790 800 810 820 830
910 920 930 940 950 960 970 980 990
AT1G68 GACTCTAGCTCCTCTTCCATTTTTGGTGACTCCGAGGAATGTTACACAAGTCTTAGTTCAGTAAGAGG---AATGTCGACACCAAGGGAACACAACACAA
:::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: ::: :: :: : ::: :::
IE_08B GACTCTAGCTCCTCTTCCATTTTTGGTGACTCCGAGGAATGTTACACAAGTCTTAGTTCAGTAAGAGGTACAATTTCAGCAGC-AGGTAAC---------
840 850 860 870 880 890 900 910 920
1000 1010 1020 1030 1040 1050 1060
AT1G68 CTTCAATAGCAACG-GTGGA-TGAGGAGAAAAGCCCCATATCATCTTTCTCTCTATATGATTATCTCTGCTAC
::::: : :: : : :::: ::: : ::
IE_08B -------AGCAATGTGTTAACT------AAAAACCCTA-AT--------------------------------
930 940 950
10 20 30 40 50 60 70 80 90 100
AT1G68 MFPSLDTNGYDLFDPFIPHQTTMFPSFITHIQSPNSHHHYSSPSFPFSSDFLESFDESFLINQFLLQQQDVAANVVESPWKFCKKLELKKKNEKCVDGST
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_08B ----------------------MFPSFITHIQSPNSHHHYSSPSFPFSSDFLESFDESFLINQFLLQQQDVAANVVESPWKFCKKLELKKKNEKCVDGST
10 20 30 40 50 60 70
110 120 130 140 150 160 170 180 190 200
AT1G68 SQEVQWRRTVKKRDRHSKICTAQGPRDRRMRLSLQIARKFFDLQDMLGFDKASKTIEWLFSKSKTSIKQLKERVAASEGGGKDEHLQVDEKEKDETLKLR
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_08B SQEVQWRRTVKKRDRHSKICTAQGPRDRRMRLSLQIARKFFDLQDMLGFDKASKTIEWLFSKSKTSIKQLKERVAASEGGGKDEHLQVDEKEKDETLKLR
80 90 100 110 120 130 140 150 160 170
210 220 230 240 250 260 270 280 290 300
AT1G68 VSKRRTKTMESSFKTKESRERARKRARERTMAKMKMRLFETSETISDPHQETREIKITNGVQLLEKENKEQEWSNTNDVHMVEYQMDSVSIIEKFLGLTS
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_08B VSKRRTKTMESSFKTKESRERARKRARERTMAKMKMRLFETSETISDPHQETREIKITNGVQLLEKENKEQEWSNTNDVHMVEYQMDSVSIIEKFLGLTS
180 190 200 210 220 230 240 250 260 270
310 320 330 340 350
AT1G68 DSSSSSIFGDSEECYTSLSSVRGMSTPREHNTTSIATVDEEKSPISSFSLYDYLCY
::::::::::::::::::::::: : ... : :.:
IE_08B DSSSSSIFGDSEECYTSLSSVRG--TISAAGNSNVLT----KNPN-----------
280 290 300 310
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