Code name | IE_08B06 | |||
---|---|---|---|---|
Locus | AT1G68800 | |||
Template | IT_08B06 (CR118) | |||
Donor Vector | pDONR207 | |||
Forward primer | IPF_08B06 (ggggacaagtttgtacaaaaaagcaggcttcATGTTTCCTTCTTTCATTACTCACATT) | |||
Reverse primer | IPR_08B06 (ggggaccactttgtacaagaaagctgggttATTAGGGTTTTTAGTTAACACATTGCTGTT) | |||
Alignment with template seq. | 1st | アミノ酸配列100%一致 | 2nd | - |
Alignment with TAIR7CDS | アミノ酸配列100%一致 | - | ||
comment | ||||
sequence |
>IE_08B06_951bp ATGTTTCCTTCTTTCATTACTCACATTCAAAGCCCTAATTCTCACCATCACTACTCTTCGCCTTCTTTTCCTTTCTCTTCCGATTTTCTTGAGAGTTTTG ATGAATCCTTCTTGATAAACCAATTCTTGTTACAGCAGCAAGATGTAGCAGCAAATGTTGTTGAATCTCCTTGGAAATTTTGCAAGAAGCTTGAGCTTAA GAAGAAGAATGAGAAGTGTGTTGATGGAAGCACCTCACAAGAGGTTCAATGGAGAAGGACGGTCAAAAAAAGGGACAGGCATAGTAAGATCTGCACGGCT CAAGGTCCTAGAGACCGGAGGATGAGGCTGTCTCTTCAGATTGCTCGCAAGTTTTTCGATCTTCAAGACATGTTGGGTTTCGACAAGGCGAGCAAGACGA TTGAATGGCTTTTCTCCAAATCAAAGACTTCCATCAAACAACTTAAAGAAAGAGTGGCTGCATCGGAAGGAGGAGGAAAGGATGAACATCTCCAGGTTGA TGAAAAGGAAAAGGATGAGACACTGAAGTTGAGAGTCTCAAAGAGAAGAACAAAGACTATGGAGAGCTCTTTTAAGACTAAAGAGTCGAGAGAGAGAGCT AGAAAGCGAGCAAGAGAGAGAACAATGGCAAAGATGAAGATGAGATTATTTGAGACCTCGGAAACAATTTCAGATCCTCATCAAGAAACTAGAGAGATCA AGATAACCAATGGTGTACAATTACTAGAAAAGGAAAATAAAGAACAAGAATGGAGTAATACTAATGATGTTCACATGGTAGAGTATCAAATGGATTCTGT GAGCATCATAGAGAAGTTTCTTGGACTAACCAGTGACTCTAGCTCCTCTTCCATTTTTGGTGACTCCGAGGAATGTTACACAAGTCTTAGTTCAGTAAGA GGTACAATTTCAGCAGCAGGTAACAGCAATGTGTTAACTAAAAACCCTAAT >IE_08B06_translated MFPSFITHIQSPNSHHHYSSPSFPFSSDFLESFDESFLINQFLLQQQDVAANVVESPWKFCKKLELKKKNEKCVDGSTSQEVQWRRTVKKRDRHSKICTA QGPRDRRMRLSLQIARKFFDLQDMLGFDKASKTIEWLFSKSKTSIKQLKERVAASEGGGKDEHLQVDEKEKDETLKLRVSKRRTKTMESSFKTKESRERA RKRARERTMAKMKMRLFETSETISDPHQETREIKITNGVQLLEKENKEQEWSNTNDVHMVEYQMDSVSIIEKFLGLTSDSSSSSIFGDSEECYTSLSSVR GTISAAGNSNVLTKNPN |
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Alignment |
◆CDS(TAIRver.9)_without_stop_codonとのalignment◆ 936/1073 (87.2%)(アミノ酸配列で305/356 (85.7%))一致しました。 10 20 30 40 50 60 70 80 90 100 AT1G68 ATGTTTCCTTCTCTAGATACCAATGGCTATGACCTCTTTGATCCCTTCATTCCCCATCAAACAACCATGTTTCCTTCTTTCATTACTCACATTCAAAGCC :::::::::::::::::::::::::::::::::: IE_08B ------------------------------------------------------------------ATGTTTCCTTCTTTCATTACTCACATTCAAAGCC 10 20 30 110 120 130 140 150 160 170 180 190 200 AT1G68 CTAATTCTCACCATCACTACTCTTCGCCTTCTTTTCCTTTCTCTTCCGATTTTCTTGAGAGTTTTGATGAATCCTTCTTGATAAACCAATTCTTGTTACA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_08B CTAATTCTCACCATCACTACTCTTCGCCTTCTTTTCCTTTCTCTTCCGATTTTCTTGAGAGTTTTGATGAATCCTTCTTGATAAACCAATTCTTGTTACA 40 50 60 70 80 90 100 110 120 130 210 220 230 240 250 260 270 280 290 300 AT1G68 GCAGCAAGATGTAGCAGCAAATGTTGTTGAATCTCCTTGGAAATTTTGCAAGAAGCTTGAGCTTAAGAAGAAGAATGAGAAGTGTGTTGATGGAAGCACC :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_08B GCAGCAAGATGTAGCAGCAAATGTTGTTGAATCTCCTTGGAAATTTTGCAAGAAGCTTGAGCTTAAGAAGAAGAATGAGAAGTGTGTTGATGGAAGCACC 140 150 160 170 180 190 200 210 220 230 310 320 330 340 350 360 370 380 390 400 AT1G68 TCACAAGAGGTTCAATGGAGAAGGACGGTCAAAAAAAGGGACAGGCATAGTAAGATCTGCACGGCTCAAGGTCCTAGAGACCGGAGGATGAGGCTGTCTC :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_08B TCACAAGAGGTTCAATGGAGAAGGACGGTCAAAAAAAGGGACAGGCATAGTAAGATCTGCACGGCTCAAGGTCCTAGAGACCGGAGGATGAGGCTGTCTC 240 250 260 270 280 290 300 310 320 330 410 420 430 440 450 460 470 480 490 500 AT1G68 TTCAGATTGCTCGCAAGTTTTTCGATCTTCAAGACATGTTGGGTTTCGACAAGGCGAGCAAGACGATTGAATGGCTTTTCTCCAAATCAAAGACTTCCAT :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_08B TTCAGATTGCTCGCAAGTTTTTCGATCTTCAAGACATGTTGGGTTTCGACAAGGCGAGCAAGACGATTGAATGGCTTTTCTCCAAATCAAAGACTTCCAT 340 350 360 370 380 390 400 410 420 430 510 520 530 540 550 560 570 580 590 600 AT1G68 CAAACAACTTAAAGAAAGAGTGGCTGCATCGGAAGGAGGAGGAAAGGATGAACATCTCCAGGTTGATGAAAAGGAAAAGGATGAGACACTGAAGTTGAGA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_08B CAAACAACTTAAAGAAAGAGTGGCTGCATCGGAAGGAGGAGGAAAGGATGAACATCTCCAGGTTGATGAAAAGGAAAAGGATGAGACACTGAAGTTGAGA 440 450 460 470 480 490 500 510 520 530 610 620 630 640 650 660 670 680 690 700 AT1G68 GTCTCAAAGAGAAGAACAAAGACTATGGAGAGCTCTTTTAAGACTAAAGAGTCGAGAGAGAGAGCTAGAAAGCGAGCAAGAGAGAGAACAATGGCAAAGA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_08B GTCTCAAAGAGAAGAACAAAGACTATGGAGAGCTCTTTTAAGACTAAAGAGTCGAGAGAGAGAGCTAGAAAGCGAGCAAGAGAGAGAACAATGGCAAAGA 540 550 560 570 580 590 600 610 620 630 710 720 730 740 750 760 770 780 790 800 AT1G68 TGAAGATGAGATTATTTGAGACCTCGGAAACAATTTCAGATCCTCATCAAGAAACTAGAGAGATCAAGATAACCAATGGTGTACAATTACTAGAAAAGGA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_08B TGAAGATGAGATTATTTGAGACCTCGGAAACAATTTCAGATCCTCATCAAGAAACTAGAGAGATCAAGATAACCAATGGTGTACAATTACTAGAAAAGGA 640 650 660 670 680 690 700 710 720 730 810 820 830 840 850 860 870 880 890 900 AT1G68 AAATAAAGAACAAGAATGGAGTAATACTAATGATGTTCACATGGTAGAGTATCAAATGGATTCTGTGAGCATCATAGAGAAGTTTCTTGGACTAACCAGT :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_08B AAATAAAGAACAAGAATGGAGTAATACTAATGATGTTCACATGGTAGAGTATCAAATGGATTCTGTGAGCATCATAGAGAAGTTTCTTGGACTAACCAGT 740 750 760 770 780 790 800 810 820 830 910 920 930 940 950 960 970 980 990 AT1G68 GACTCTAGCTCCTCTTCCATTTTTGGTGACTCCGAGGAATGTTACACAAGTCTTAGTTCAGTAAGAGG---AATGTCGACACCAAGGGAACACAACACAA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: ::: :: :: : ::: ::: IE_08B GACTCTAGCTCCTCTTCCATTTTTGGTGACTCCGAGGAATGTTACACAAGTCTTAGTTCAGTAAGAGGTACAATTTCAGCAGC-AGGTAAC--------- 840 850 860 870 880 890 900 910 920 1000 1010 1020 1030 1040 1050 1060 AT1G68 CTTCAATAGCAACG-GTGGA-TGAGGAGAAAAGCCCCATATCATCTTTCTCTCTATATGATTATCTCTGCTAC ::::: : :: : : :::: ::: : :: IE_08B -------AGCAATGTGTTAACT------AAAAACCCTA-AT-------------------------------- 930 940 950 10 20 30 40 50 60 70 80 90 100 AT1G68 MFPSLDTNGYDLFDPFIPHQTTMFPSFITHIQSPNSHHHYSSPSFPFSSDFLESFDESFLINQFLLQQQDVAANVVESPWKFCKKLELKKKNEKCVDGST :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_08B ----------------------MFPSFITHIQSPNSHHHYSSPSFPFSSDFLESFDESFLINQFLLQQQDVAANVVESPWKFCKKLELKKKNEKCVDGST 10 20 30 40 50 60 70 110 120 130 140 150 160 170 180 190 200 AT1G68 SQEVQWRRTVKKRDRHSKICTAQGPRDRRMRLSLQIARKFFDLQDMLGFDKASKTIEWLFSKSKTSIKQLKERVAASEGGGKDEHLQVDEKEKDETLKLR :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_08B SQEVQWRRTVKKRDRHSKICTAQGPRDRRMRLSLQIARKFFDLQDMLGFDKASKTIEWLFSKSKTSIKQLKERVAASEGGGKDEHLQVDEKEKDETLKLR 80 90 100 110 120 130 140 150 160 170 210 220 230 240 250 260 270 280 290 300 AT1G68 VSKRRTKTMESSFKTKESRERARKRARERTMAKMKMRLFETSETISDPHQETREIKITNGVQLLEKENKEQEWSNTNDVHMVEYQMDSVSIIEKFLGLTS :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_08B VSKRRTKTMESSFKTKESRERARKRARERTMAKMKMRLFETSETISDPHQETREIKITNGVQLLEKENKEQEWSNTNDVHMVEYQMDSVSIIEKFLGLTS 180 190 200 210 220 230 240 250 260 270 310 320 330 340 350 AT1G68 DSSSSSIFGDSEECYTSLSSVRGMSTPREHNTTSIATVDEEKSPISSFSLYDYLCY ::::::::::::::::::::::: : ... : :.: IE_08B DSSSSSIFGDSEECYTSLSSVRG--TISAAGNSNVLT----KNPN----------- 280 290 300 310 |