◆◆◆IE_08B11 detail◆◆◆

Code name	IE_08B11
Locus	AT1G56010
Template	IT_08B11 (CR203)
Donor Vector	pDONR207
Forward primer	IPF_08B11 (ggggacaagtttgtacaaaaaagcaggcttcATGGAGACGGAAGAAGAGATGAA)
Reverse primer	IPR_08B11 (ggggaccactttgtacaagaaagctgggttGCAATTCCAAACAGTGCTTGGAATACCGAT)
Alignment with template seq.	1st	アミノ酸配列100%一致	2nd	-
Alignment with TAIR7CDS	1st	アミノ酸配列79.30%一致	2nd	-
comment	1st:Templateの配列とは一致
sequence	>IE_08B11_972bp ATGGAGACGGAAGAAGAGATGAAGGAAAGTAGTATAAGCATGGTGGAGGCAAAGTTGCCTCCGGGATTCAGATTTCACCCGAAGGACGATGAGCTTGTCT GCGATTACTTGATGAGACGATCGCTTCACAATAATCATCGACCACCTCTTGTCCTGATCCAAGTCGATCTCAACAAGTGTGAGCCTTGGGACATCCCAAA AATGGCATGCGTGGGAGGGAAGGATTGGTATTTCTACAGCCAAAGAGACCGAAAATACGCGACGGGGCTGAGAACTAACCGAGCAACGGCCACCGGATAT TGGAAAGCCACCGGCAAAGACAGAACCATTCTAAGAAAGGGTAAGCTAGTTGGGATGAGGAAGACATTGGTTTTCTATCAAGGTCGAGCTCCTCGAGGCC GTAAAACCGATTGGGTCATGCACGAATTCCGTCTCCAAGGATCTCATCATCCTCCCAATCATTCTCTGAGCTCTCCAAAGGAAGACTGGGTCTTGTGTAG GGTATTCCATAAGAATACGGAAGGAGTTATATGTAGAGACAACATGGGAAGCTGTTTTGATGAGACAGCCTCTGCATCGCTTCCTCCACTGATGGATCCT TACATCAACTTTGACCAAGAACCCTCTTCTTATCTCAGTGATGATCATCACTACATCATCAATGAGCACGTACCCTGCTTCTCCAATTTGTCACAGAACC AAACCTTAAACTCGAACCTAACCAACTCAGTCTCTGAACTCAAGATTCCATGCAAGAACCCTAACCCCTTGTTTACTGGTGGTTCAGCCTCAGCCACGCT CACAGGCCTCGACTCATTCTGTTCTTCAGATCAGATGGTTCTCAGAGCTCTACTCAGTCAGCTCACTAAGATTGATGGAAGCCTCGGGCCTAAAGAATCA CAGAGTTATGGAGAAGGTAGCTCGGAGAGCCTCCTGACCGACATCGGTATTCCAAGCACTGTTTGGAATTGC >IE_08B11_translated METEEEMKESSISMVEAKLPPGFRFHPKDDELVCDYLMRRSLHNNHRPPLVLIQVDLNKCEPWDIPKMACVGGKDWYFYSQRDRKYATGLRTNRATATGY WKATGKDRTILRKGKLVGMRKTLVFYQGRAPRGRKTDWVMHEFRLQGSHHPPNHSLSSPKEDWVLCRVFHKNTEGVICRDNMGSCFDETASASLPPLMDP YINFDQEPSSYLSDDHHYIINEHVPCFSNLSQNQTLNSNLTNSVSELKIPCKNPNPLFTGGSASATLTGLDSFCSSDQMVLRALLSQLTKIDGSLGPKES QSYGEGSSESLLTDIGIPSTVWNC
Alignment	◆CDS（TAIRver.9）_without_stop_codonとのalignment◆ 771/972 (79.3%)（アミノ酸配列で257/324 (79.3%)）一致しました。 AT1G56 ---------------------------------------------------------------------------------------------------- IE_08B ATGGAGACGGAAGAAGAGATGAAGGAAAGTAGTATAAGCATGGTGGAGGCAAAGTTGCCTCCGGGATTCAGATTTCACCCGAAGGACGATGAGCTTGTCT 10 20 30 40 50 60 70 80 90 100 AT1G56 ---------------------------------------------------------------------------------------------------- IE_08B GCGATTACTTGATGAGACGATCGCTTCACAATAATCATCGACCACCTCTTGTCCTGATCCAAGTCGATCTCAACAAGTGTGAGCCTTGGGACATCCCAAA 110 120 130 140 150 160 170 180 190 200 10 20 30 40 50 60 70 80 90 AT1G56 -ATGGCATGCGTGGGAGGGAAGGATTGGTATTTCTACAGCCAAAGAGACCGAAAATACGCGACGGGGCTGAGAACTAACCGAGCAACGGCCACCGGATAT ::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_08B AATGGCATGCGTGGGAGGGAAGGATTGGTATTTCTACAGCCAAAGAGACCGAAAATACGCGACGGGGCTGAGAACTAACCGAGCAACGGCCACCGGATAT 210 220 230 240 250 260 270 280 290 300 100 110 120 130 140 150 160 170 180 190 AT1G56 TGGAAAGCCACCGGCAAAGACAGAACCATTCTAAGAAAGGGTAAGCTAGTTGGGATGAGGAAGACATTGGTTTTCTATCAAGGTCGAGCTCCTCGAGGCC :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_08B TGGAAAGCCACCGGCAAAGACAGAACCATTCTAAGAAAGGGTAAGCTAGTTGGGATGAGGAAGACATTGGTTTTCTATCAAGGTCGAGCTCCTCGAGGCC 310 320 330 340 350 360 370 380 390 400 200 210 220 230 240 250 260 270 280 290 AT1G56 GTAAAACCGATTGGGTCATGCACGAATTCCGTCTCCAAGGATCTCATCATCCTCCCAATCATTCTCTGAGCTCTCCAAAGGAAGACTGGGTCTTGTGTAG :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_08B GTAAAACCGATTGGGTCATGCACGAATTCCGTCTCCAAGGATCTCATCATCCTCCCAATCATTCTCTGAGCTCTCCAAAGGAAGACTGGGTCTTGTGTAG 410 420 430 440 450 460 470 480 490 500 300 310 320 330 340 350 360 370 380 390 AT1G56 GGTATTCCATAAGAATACGGAAGGAGTTATATGTAGAGACAACATGGGAAGCTGTTTTGATGAGACAGCCTCTGCATCGCTTCCTCCACTGATGGATCCT :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_08B GGTATTCCATAAGAATACGGAAGGAGTTATATGTAGAGACAACATGGGAAGCTGTTTTGATGAGACAGCCTCTGCATCGCTTCCTCCACTGATGGATCCT 510 520 530 540 550 560 570 580 590 600 400 410 420 430 440 450 460 470 480 490 AT1G56 TACATCAACTTTGACCAAGAACCCTCTTCTTATCTCAGTGATGATCATCACTACATCATCAATGAGCACGTACCCTGCTTCTCCAATTTGTCACAGAACC :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_08B TACATCAACTTTGACCAAGAACCCTCTTCTTATCTCAGTGATGATCATCACTACATCATCAATGAGCACGTACCCTGCTTCTCCAATTTGTCACAGAACC 610 620 630 640 650 660 670 680 690 700 500 510 520 530 540 550 560 570 580 590 AT1G56 AAACCTTAAACTCGAACCTAACCAACTCAGTCTCTGAACTCAAGATTCCATGCAAGAACCCTAACCCCTTGTTTACTGGTGGTTCAGCCTCAGCCACGCT :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_08B AAACCTTAAACTCGAACCTAACCAACTCAGTCTCTGAACTCAAGATTCCATGCAAGAACCCTAACCCCTTGTTTACTGGTGGTTCAGCCTCAGCCACGCT 710 720 730 740 750 760 770 780 790 800 600 610 620 630 640 650 660 670 680 690 AT1G56 CACAGGCCTCGACTCATTCTGTTCTTCAGATCAGATGGTTCTCAGAGCTCTACTCAGTCAGCTCACTAAGATTGATGGAAGCCTCGGGCCTAAAGAATCA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_08B CACAGGCCTCGACTCATTCTGTTCTTCAGATCAGATGGTTCTCAGAGCTCTACTCAGTCAGCTCACTAAGATTGATGGAAGCCTCGGGCCTAAAGAATCA 810 820 830 840 850 860 870 880 890 900 700 710 720 730 740 750 760 770 AT1G56 CAGAGTTATGGAGAAGGTAGCTCGGAGAGCCTCCTGACCGACATCGGTATTCCAAGCACTGTTTGGAATTGC :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_08B CAGAGTTATGGAGAAGGTAGCTCGGAGAGCCTCCTGACCGACATCGGTATTCCAAGCACTGTTTGGAATTGC 910 920 930 940 950 960 970 10 20 30 AT1G56 -------------------------------------------------------------------MACVGGKDWYFYSQRDRKYATGLRTNRATATGY ::::::::::::::::::::::::::::::::: IE_08B METEEEMKESSISMVEAKLPPGFRFHPKDDELVCDYLMRRSLHNNHRPPLVLIQVDLNKCEPWDIPKMACVGGKDWYFYSQRDRKYATGLRTNRATATGY 10 20 30 40 50 60 70 80 90 100 40 50 60 70 80 90 100 110 120 130 AT1G56 WKATGKDRTILRKGKLVGMRKTLVFYQGRAPRGRKTDWVMHEFRLQGSHHPPNHSLSSPKEDWVLCRVFHKNTEGVICRDNMGSCFDETASASLPPLMDP :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_08B WKATGKDRTILRKGKLVGMRKTLVFYQGRAPRGRKTDWVMHEFRLQGSHHPPNHSLSSPKEDWVLCRVFHKNTEGVICRDNMGSCFDETASASLPPLMDP 110 120 130 140 150 160 170 180 190 200 140 150 160 170 180 190 200 210 220 230 AT1G56 YINFDQEPSSYLSDDHHYIINEHVPCFSNLSQNQTLNSNLTNSVSELKIPCKNPNPLFTGGSASATLTGLDSFCSSDQMVLRALLSQLTKIDGSLGPKES :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_08B YINFDQEPSSYLSDDHHYIINEHVPCFSNLSQNQTLNSNLTNSVSELKIPCKNPNPLFTGGSASATLTGLDSFCSSDQMVLRALLSQLTKIDGSLGPKES 210 220 230 240 250 260 270 280 290 300 240 250 AT1G56 QSYGEGSSESLLTDIGIPSTVWNC :::::::::::::::::::::::: IE_08B QSYGEGSSESLLTDIGIPSTVWNC 310 320

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