◆◆◆IE_08C01 detail◆◆◆

Code nameIE_08C01
LocusAT1G52880
TemplateIT_08C01 (CR248)
Donor VectorpDONR207
Forward primerIPF_08C01 (ggggacaagtttgtacaaaaaagcaggcttcATGGAGAGTACAGATTCTTCCGGTGGT)
Reverse primerIPR_08C01 (ggggaccactttgtacaagaaagctgggttAGAATACCAATTCAAACCAGGCAATTGGTA)
Alignment with template seq.1stアミノ酸配列99.70%一致2ndアミノ酸配列99.70%一致
Alignment with TAIR7CDSアミノ酸配列99.70%一致アミノ酸配列99.70%一致
comment1st:(Sequence解析まで行ったが、フレームはずれないが塩基置換がある), 2nd:70%, nt.617A→G
sequence 
>IE_08C01_960bp
ATGGAGAGTACAGATTCTTCCGGTGGTCCTCCGCCGCCGCAACCAAACCTCCCTCCAGGATTCCGGTTTCATCCAACAGACGAAGAACTTGTAATTCATT
ACCTCAAACGCAAAGCAGATTCTGTTCCTTTACCAGTCGCGATCATCGCCGACGTTGATCTTTACAAATTTGATCCATGGGAACTTCCCGCGAAAGCTTC
GTTTGGAGAACAAGAATGGTATTTTTTCAGTCCAAGAGATCGGAAATATCCCAACGGAGCTAGACCTAACCGAGCTGCGACTTCCGGTTATTGGAAAGCG
ACTGGTACAGATAAACCGGTGATTTCAACCGGCGGTGGTGGTAGTAAAAAAGTGGGAGTTAAAAAGGCTCTAGTGTTTTACAGTGGTAAACCACCAAAAG
GAGTTAAATCAGATTGGATTATGCATGAATATCGGTTAACTGATAATAAACCTACTCATATTTGTGACTTCGGCAACAAGAAAAACTCTCTCAGGCTTGA
TGATTGGGTGTTGTGTCGTATCTACAAGAAAAACAATAGTACAGCATCTAGACATCATCATCATCTTCATCATATTCATCTAGATAATGATCATCATCGT
CATGATATGATGATTGGTGATGATCGATTCCGTCATGTTCCTCCTGGTCTTCACTTCCCGGCGATTTTTTCTGACAATAATGATCCGACGGCTATATATG
ATGGTGGCGGCGGCGGATACGGAGGTGGAAGTTACTCGATGAATCATTGTTTCGCATCTGGATCAAAGCAGGAGCAGTTGTTTCCACCGGTGATGATGAT
GACTAGTCTAAATCAAGATTCCGGTATTGGATCGTCGTCGTCACCTAGCAAGAGATTTAACGGCGGCGGCGTTGGAGATTGTTCGACTTCTATGGCGGCG
ACGCCGTTAATGCAGAACCAAGGTGGGATTTACCAATTGCCTGGTTTGAATTGGTATTCT

>IE_08C01_translated
MESTDSSGGPPPPQPNLPPGFRFHPTDEELVIHYLKRKADSVPLPVAIIADVDLYKFDPWELPAKASFGEQEWYFFSPRDRKYPNGARPNRAATSGYWKA
TGTDKPVISTGGGGSKKVGVKKALVFYSGKPPKGVKSDWIMHEYRLTDNKPTHICDFGNKKNSLRLDDWVLCRIYKKNNSTASRHHHHLHHIHLDNDHHR
HDMMIGDDRFRHVPPGLHFPAIFSDNNDPTAIYDGGGGGYGGGSYSMNHCFASGSKQEQLFPPVMMMTSLNQDSGIGSSSSPSKRFNGGGVGDCSTSMAA
TPLMQNQGGIYQLPGLNWYS
Alignment ◆CDS（TAIRver.9）_without_stop_codonとのalignment◆

959/960 (99.9%)（アミノ酸配列で319/320 (99.7%)）一致しました。


               10        20        30        40        50        60        70        80        90       100
AT1G52 ATGGAGAGTACAGATTCTTCCGGTGGTCCTCCGCCGCCGCAACCAAACCTCCCTCCAGGATTCCGGTTTCATCCAACAGACGAAGAACTTGTAATTCATT
       ::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_08C ATGGAGAGTACAGATTCTTCCGGTGGTCCTCCGCCGCCGCAACCAAACCTCCCTCCAGGATTCCGGTTTCATCCAACAGACGAAGAACTTGTAATTCATT
               10        20        30        40        50        60        70        80        90       100

              110       120       130       140       150       160       170       180       190       200
AT1G52 ACCTCAAACGCAAAGCAGATTCTGTTCCTTTACCAGTCGCGATCATCGCCGACGTTGATCTTTACAAATTTGATCCATGGGAACTTCCCGCGAAAGCTTC
       ::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_08C ACCTCAAACGCAAAGCAGATTCTGTTCCTTTACCAGTCGCGATCATCGCCGACGTTGATCTTTACAAATTTGATCCATGGGAACTTCCCGCGAAAGCTTC
              110       120       130       140       150       160       170       180       190       200

              210       220       230       240       250       260       270       280       290       300
AT1G52 GTTTGGAGAACAAGAATGGTATTTTTTCAGTCCAAGAGATCGGAAATATCCCAACGGAGCTAGACCTAACCGAGCTGCGACTTCCGGTTATTGGAAAGCG
       ::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_08C GTTTGGAGAACAAGAATGGTATTTTTTCAGTCCAAGAGATCGGAAATATCCCAACGGAGCTAGACCTAACCGAGCTGCGACTTCCGGTTATTGGAAAGCG
              210       220       230       240       250       260       270       280       290       300

              310       320       330       340       350       360       370       380       390       400
AT1G52 ACTGGTACAGATAAACCGGTGATTTCAACCGGCGGTGGTGGTAGTAAAAAAGTGGGAGTTAAAAAGGCTCTAGTGTTTTACAGTGGTAAACCACCAAAAG
       ::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_08C ACTGGTACAGATAAACCGGTGATTTCAACCGGCGGTGGTGGTAGTAAAAAAGTGGGAGTTAAAAAGGCTCTAGTGTTTTACAGTGGTAAACCACCAAAAG
              310       320       330       340       350       360       370       380       390       400

              410       420       430       440       450       460       470       480       490       500
AT1G52 GAGTTAAATCAGATTGGATTATGCATGAATATCGGTTAACTGATAATAAACCTACTCATATTTGTGACTTCGGCAACAAGAAAAACTCTCTCAGGCTTGA
       ::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_08C GAGTTAAATCAGATTGGATTATGCATGAATATCGGTTAACTGATAATAAACCTACTCATATTTGTGACTTCGGCAACAAGAAAAACTCTCTCAGGCTTGA
              410       420       430       440       450       460       470       480       490       500

              510       520       530       540       550       560       570       580       590       600
AT1G52 TGATTGGGTGTTGTGTCGTATCTACAAGAAAAACAATAGTACAGCATCTAGACATCATCATCATCTTCATCATATTCATCTAGATAATGATCATCATCGT
       ::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_08C TGATTGGGTGTTGTGTCGTATCTACAAGAAAAACAATAGTACAGCATCTAGACATCATCATCATCTTCATCATATTCATCTAGATAATGATCATCATCGT
              510       520       530       540       550       560       570       580       590       600

              610       620       630       640       650       660       670       680       690       700
AT1G52 CATGATATGATGATTGATGATGATCGATTCCGTCATGTTCCTCCTGGTCTTCACTTCCCGGCGATTTTTTCTGACAATAATGATCCGACGGCTATATATG
       :::::::::::::::: :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_08C CATGATATGATGATTGGTGATGATCGATTCCGTCATGTTCCTCCTGGTCTTCACTTCCCGGCGATTTTTTCTGACAATAATGATCCGACGGCTATATATG
              610       620       630       640       650       660       670       680       690       700

              710       720       730       740       750       760       770       780       790       800
AT1G52 ATGGTGGCGGCGGCGGATACGGAGGTGGAAGTTACTCGATGAATCATTGTTTCGCATCTGGATCAAAGCAGGAGCAGTTGTTTCCACCGGTGATGATGAT
       ::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_08C ATGGTGGCGGCGGCGGATACGGAGGTGGAAGTTACTCGATGAATCATTGTTTCGCATCTGGATCAAAGCAGGAGCAGTTGTTTCCACCGGTGATGATGAT
              710       720       730       740       750       760       770       780       790       800

              810       820       830       840       850       860       870       880       890       900
AT1G52 GACTAGTCTAAATCAAGATTCCGGTATTGGATCGTCGTCGTCACCTAGCAAGAGATTTAACGGCGGCGGCGTTGGAGATTGTTCGACTTCTATGGCGGCG
       ::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_08C GACTAGTCTAAATCAAGATTCCGGTATTGGATCGTCGTCGTCACCTAGCAAGAGATTTAACGGCGGCGGCGTTGGAGATTGTTCGACTTCTATGGCGGCG
              810       820       830       840       850       860       870       880       890       900

              910       920       930       940       950       960
AT1G52 ACGCCGTTAATGCAGAACCAAGGTGGGATTTACCAATTGCCTGGTTTGAATTGGTATTCT
       ::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_08C ACGCCGTTAATGCAGAACCAAGGTGGGATTTACCAATTGCCTGGTTTGAATTGGTATTCT
              910       920       930       940       950       960




               10        20        30        40        50        60        70        80        90       100
AT1G52 MESTDSSGGPPPPQPNLPPGFRFHPTDEELVIHYLKRKADSVPLPVAIIADVDLYKFDPWELPAKASFGEQEWYFFSPRDRKYPNGARPNRAATSGYWKA
       ::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_08C MESTDSSGGPPPPQPNLPPGFRFHPTDEELVIHYLKRKADSVPLPVAIIADVDLYKFDPWELPAKASFGEQEWYFFSPRDRKYPNGARPNRAATSGYWKA
               10        20        30        40        50        60        70        80        90       100

              110       120       130       140       150       160       170       180       190       200
AT1G52 TGTDKPVISTGGGGSKKVGVKKALVFYSGKPPKGVKSDWIMHEYRLTDNKPTHICDFGNKKNSLRLDDWVLCRIYKKNNSTASRHHHHLHHIHLDNDHHR
       ::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_08C TGTDKPVISTGGGGSKKVGVKKALVFYSGKPPKGVKSDWIMHEYRLTDNKPTHICDFGNKKNSLRLDDWVLCRIYKKNNSTASRHHHHLHHIHLDNDHHR
              110       120       130       140       150       160       170       180       190       200

              210       220       230       240       250       260       270       280       290       300
AT1G52 HDMMIDDDRFRHVPPGLHFPAIFSDNNDPTAIYDGGGGGYGGGSYSMNHCFASGSKQEQLFPPVMMMTSLNQDSGIGSSSSPSKRFNGGGVGDCSTSMAA
       ::::: ::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_08C HDMMIGDDRFRHVPPGLHFPAIFSDNNDPTAIYDGGGGGYGGGSYSMNHCFASGSKQEQLFPPVMMMTSLNQDSGIGSSSSPSKRFNGGGVGDCSTSMAA
              210       220       230       240       250       260       270       280       290       300

              310       320
AT1G52 TPLMQNQGGIYQLPGLNWYS
       ::::::::::::::::::::
IE_08C TPLMQNQGGIYQLPGLNWYS
              310       320

このページのTOPに戻る