◆◆◆IE_08D05 detail◆◆◆

Code nameIE_08D05
LocusAT1G12980
TemplateIT_08D05 (CR597(TP)
Donor VectorpDONR207
Forward primerIPF_08D05 (ggggacaagtttgtacaaaaaagcaggcttcATGGAAAAAGCCTTGAGAAACTTCACC)
Reverse primerIPR_08D05 (ggggaccactttgtacaagaaagctgggttTCCCCACGATCTTCGGCAAGTACAGCCTAA)
Alignment with template seq.1stアミノ酸配列99.70%一致2ndアミノ酸配列99.70%一致
Alignment with TAIR7CDSアミノ酸配列99.70%一致アミノ酸配列99.70%一致
comment1st:(Sequence解析まで行ったが、フレームはずれないが塩基置換がある), 2nd:70%, nt.543G→C, nt.775A→G, nt.776A→C, nt.903G→T
sequence 
>IE_08D05_984bp
ATGGAAAAAGCCTTGAGAAACTTCACCGAATCTACCCACTCACCAGACCCTAATCCTCTCACAAAATTCTTCACTGAACCTACAGCCTCACCTGTTAGCC
GCAACCGCAAACTGTCTTCAAAAGATACCACTGTAACCATCGCCGGAGCTGGCAGCAGCACGACGAGGTACCGCGGCGTACGCCGGAGGCCGTGGGGACG
ATACGCGGCGGAGATACGTGACCCAATGTCGAAGGAGAGACGTTGGCTCGGAACATTTGACACGGCGGAACAAGCCGCTTGTGCTTACGACTCTGCGGCT
CGTGCCTTTCGTGGAGCAAAGGCTCGTACTAATTTTACTTATCCGACAGCTGTCATTATGCCTGAACCAAGGTTTTCTTTTTCCAACAAGAAATCTTCGC
CGTCTGCTCGTTGTCCTCTTCCTTCTCTACCGTTAGATTCCTCTACCCAAAACTTTTACGGTGCACCGGCAGCGCAGAGGATCTATAATACACAGTCTAT
CTTCTTACGCGACGCCTCGTGTTCCTCTCGTAAAACGACTCCCTATAATAACTCTTTCAACGGCTCATCATCTTCTTACTCAGCATCGAAAACGGCATGC
GTTTCTTATTCCGAAAACGAAAACAACGAGTCGTTTTTCCCGGAAGAATCTTCTGATACTGGTCTATTACAAGAGGTCGTTCAAGAGTTCTTGAAGAAAA
ATCGCGGCGTTCCTCCTTCTCCACCAACACCACCGCCGGTGACTAGCCATCATGACAACTCTGGTTATTTCTCTGCTCTCACTATATACTCTGAAAATAT
GGTTCAAGAGACTAAGGAGACTTTGTCGTCGAAACTAGATCGCTACGGGAATTTTCAAGCTAATGACGACGGCGTAAGAGCCGTCGCAGACGGTGGTTTA
TCTTTGGGATCAAACGAGTGGGGGTATCAAGAAATGTTGATGTACGGAACTCAGTTAGGCTGTACTTGCCGAAGATCGTGGGGA

>IE_08D05_translated
MEKALRNFTESTHSPDPNPLTKFFTEPTASPVSRNRKLSSKDTTVTIAGAGSSTTRYRGVRRRPWGRYAAEIRDPMSKERRWLGTFDTAEQAACAYDSAA
RAFRGAKARTNFTYPTAVIMPEPRFSFSNKKSSPSARCPLPSLPLDSSTQNFYGAPAAQRIYNTQSIFLRDASCSSRKTTPYNNSFNGSSSSYSASKTAC
VSYSENENNESFFPEESSDTGLLQEVVQEFLKKNRGVPPSPPTPPPVTSHHDNSGYFSALTIYSENMVQETKETLSSKLDRYGNFQANDDGVRAVADGGL
SLGSNEWGYQEMLMYGTQLGCTCRRSWG
Alignment ◆CDS（TAIRver.9）_without_stop_codonとのalignment◆

980/984 (99.6%)（アミノ酸配列で327/328 (99.7%)）一致しました。


               10        20        30        40        50        60        70        80        90       100
AT1G12 ATGGAAAAAGCCTTGAGAAACTTCACCGAATCTACCCACTCACCAGACCCTAATCCTCTCACAAAATTCTTCACTGAACCTACAGCCTCACCTGTTAGCC
       ::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_08D ATGGAAAAAGCCTTGAGAAACTTCACCGAATCTACCCACTCACCAGACCCTAATCCTCTCACAAAATTCTTCACTGAACCTACAGCCTCACCTGTTAGCC
               10        20        30        40        50        60        70        80        90       100

              110       120       130       140       150       160       170       180       190       200
AT1G12 GCAACCGCAAACTGTCTTCAAAAGATACCACTGTAACCATCGCCGGAGCTGGCAGCAGCACGACGAGGTACCGCGGCGTACGCCGGAGGCCGTGGGGACG
       ::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_08D GCAACCGCAAACTGTCTTCAAAAGATACCACTGTAACCATCGCCGGAGCTGGCAGCAGCACGACGAGGTACCGCGGCGTACGCCGGAGGCCGTGGGGACG
              110       120       130       140       150       160       170       180       190       200

              210       220       230       240       250       260       270       280       290       300
AT1G12 ATACGCGGCGGAGATACGTGACCCAATGTCGAAGGAGAGACGTTGGCTCGGAACATTTGACACGGCGGAACAAGCCGCTTGTGCTTACGACTCTGCGGCT
       ::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_08D ATACGCGGCGGAGATACGTGACCCAATGTCGAAGGAGAGACGTTGGCTCGGAACATTTGACACGGCGGAACAAGCCGCTTGTGCTTACGACTCTGCGGCT
              210       220       230       240       250       260       270       280       290       300

              310       320       330       340       350       360       370       380       390       400
AT1G12 CGTGCCTTTCGTGGAGCAAAGGCTCGTACTAATTTTACTTATCCGACAGCTGTCATTATGCCTGAACCAAGGTTTTCTTTTTCCAACAAGAAATCTTCGC
       ::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_08D CGTGCCTTTCGTGGAGCAAAGGCTCGTACTAATTTTACTTATCCGACAGCTGTCATTATGCCTGAACCAAGGTTTTCTTTTTCCAACAAGAAATCTTCGC
              310       320       330       340       350       360       370       380       390       400

              410       420       430       440       450       460       470       480       490       500
AT1G12 CGTCTGCTCGTTGTCCTCTTCCTTCTCTACCGTTAGATTCCTCTACCCAAAACTTTTACGGTGCACCGGCAGCGCAGAGGATCTATAATACACAGTCTAT
       ::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_08D CGTCTGCTCGTTGTCCTCTTCCTTCTCTACCGTTAGATTCCTCTACCCAAAACTTTTACGGTGCACCGGCAGCGCAGAGGATCTATAATACACAGTCTAT
              410       420       430       440       450       460       470       480       490       500

              510       520       530       540       550       560       570       580       590       600
AT1G12 CTTCTTACGCGACGCCTCGTGTTCCTCTCGTAAAACGACTCCGTATAATAACTCTTTCAACGGCTCATCATCTTCTTACTCAGCATCGAAAACGGCATGC
       :::::::::::::::::::::::::::::::::::::::::: :::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_08D CTTCTTACGCGACGCCTCGTGTTCCTCTCGTAAAACGACTCCCTATAATAACTCTTTCAACGGCTCATCATCTTCTTACTCAGCATCGAAAACGGCATGC
              510       520       530       540       550       560       570       580       590       600

              610       620       630       640       650       660       670       680       690       700
AT1G12 GTTTCTTATTCCGAAAACGAAAACAACGAGTCGTTTTTCCCGGAAGAATCTTCTGATACTGGTCTATTACAAGAGGTCGTTCAAGAGTTCTTGAAGAAAA
       ::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_08D GTTTCTTATTCCGAAAACGAAAACAACGAGTCGTTTTTCCCGGAAGAATCTTCTGATACTGGTCTATTACAAGAGGTCGTTCAAGAGTTCTTGAAGAAAA
              610       620       630       640       650       660       670       680       690       700

              710       720       730       740       750       760       770       780       790       800
AT1G12 ATCGCGGCGTTCCTCCTTCTCCACCAACACCACCGCCGGTGACTAGCCATCATGACAACTCTGGTTATTTCTCTAATCTCACTATATACTCTGAAAATAT
       ::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::  ::::::::::::::::::::::::
IE_08D ATCGCGGCGTTCCTCCTTCTCCACCAACACCACCGCCGGTGACTAGCCATCATGACAACTCTGGTTATTTCTCTGCTCTCACTATATACTCTGAAAATAT
              710       720       730       740       750       760       770       780       790       800

              810       820       830       840       850       860       870       880       890       900
AT1G12 GGTTCAAGAGACTAAGGAGACTTTGTCGTCGAAACTAGATCGCTACGGGAATTTTCAAGCTAATGACGACGGCGTAAGAGCCGTCGCAGACGGTGGTTTA
       ::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_08D GGTTCAAGAGACTAAGGAGACTTTGTCGTCGAAACTAGATCGCTACGGGAATTTTCAAGCTAATGACGACGGCGTAAGAGCCGTCGCAGACGGTGGTTTA
              810       820       830       840       850       860       870       880       890       900

              910       920       930       940       950       960       970       980    
AT1G12 TCGTTGGGATCAAACGAGTGGGGGTATCAAGAAATGTTGATGTACGGAACTCAGTTAGGCTGTACTTGCCGAAGATCGTGGGGA
       :: :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_08D TCTTTGGGATCAAACGAGTGGGGGTATCAAGAAATGTTGATGTACGGAACTCAGTTAGGCTGTACTTGCCGAAGATCGTGGGGA
              910       920       930       940       950       960       970       980    




               10        20        30        40        50        60        70        80        90       100
AT1G12 MEKALRNFTESTHSPDPNPLTKFFTEPTASPVSRNRKLSSKDTTVTIAGAGSSTTRYRGVRRRPWGRYAAEIRDPMSKERRWLGTFDTAEQAACAYDSAA
       ::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_08D MEKALRNFTESTHSPDPNPLTKFFTEPTASPVSRNRKLSSKDTTVTIAGAGSSTTRYRGVRRRPWGRYAAEIRDPMSKERRWLGTFDTAEQAACAYDSAA
               10        20        30        40        50        60        70        80        90       100

              110       120       130       140       150       160       170       180       190       200
AT1G12 RAFRGAKARTNFTYPTAVIMPEPRFSFSNKKSSPSARCPLPSLPLDSSTQNFYGAPAAQRIYNTQSIFLRDASCSSRKTTPYNNSFNGSSSSYSASKTAC
       ::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_08D RAFRGAKARTNFTYPTAVIMPEPRFSFSNKKSSPSARCPLPSLPLDSSTQNFYGAPAAQRIYNTQSIFLRDASCSSRKTTPYNNSFNGSSSSYSASKTAC
              110       120       130       140       150       160       170       180       190       200

              210       220       230       240       250       260       270       280       290       300
AT1G12 VSYSENENNESFFPEESSDTGLLQEVVQEFLKKNRGVPPSPPTPPPVTSHHDNSGYFSNLTIYSENMVQETKETLSSKLDRYGNFQANDDGVRAVADGGL
       :::::::::::::::::::::::::::::::::::::::::::::::::::::::::: :::::::::::::::::::::::::::::::::::::::::
IE_08D VSYSENENNESFFPEESSDTGLLQEVVQEFLKKNRGVPPSPPTPPPVTSHHDNSGYFSALTIYSENMVQETKETLSSKLDRYGNFQANDDGVRAVADGGL
              210       220       230       240       250       260       270       280       290       300

              310       320        
AT1G12 SLGSNEWGYQEMLMYGTQLGCTCRRSWG
       ::::::::::::::::::::::::::::
IE_08D SLGSNEWGYQEMLMYGTQLGCTCRRSWG
              310       320

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