| Code name | IE_08D07 | |||
|---|---|---|---|---|
| Locus | AT1G60240 | |||
| Template | IT_08D07 (CR623) | |||
| Donor Vector | pDONR207 | |||
| Forward primer | IPF_08D07 (ggggacaagtttgtacaaaaaagcaggcttcATGAAGTCAAGACGTGAACAATCAATCG) | |||
| Reverse primer | IPR_08D07 (ggggaccactttgtacaagaaagctgggttTTTATAGTAACCTCGAATGTGCTGGGCCAA) | |||
| Alignment with template seq. | 1st | アミノ酸配列100%一致 | 2nd | - |
| Alignment with TAIR7CDS | アミノ酸配列99.40%一致 | - | ||
| comment | 1st:Templateの配列とは一致 | |||
| sequence |
>IE_08D07_522bp ATGAAGTCAAGACGTGAACAATCAATCGAAGAAGCAATCGTCGCAAATTATTTGAAGATGATGATCGATAACGTAAACGTTTGGCCTCGCCACTTCCTCC GAAGCGAAGACGTGTACTGCAAGAATCCGTGGACGCTTTTCGTTACTAGAGATCCTATAATCCTACACTTCGGACGATACTTCTTCGTTAATCGGAGTGT GAATTCAGGTTTAACCGATGGATGTGAATACGGTTGTTGGAGAATCATCGGTCGTGATAGAGTGATCAAGTCGGTGACGACCGGGAAGATTCTAGGGTTA AAGAAGGTTTATAAGTTCTGTGAAACTGATCGGAAACCGAAATCGGTTTTTAAGTTCTTGGAAAAGGAGAAGAGAAGAGTAAGAGATAGACGAATCTGGG CGATGGAAGAGTATAGGTTTGCAAGTACGTGGAAACAAGATTACGTGATCTGCAAGATTCGACGTCTGTATCCACAACCATTTGACTACATGTTGGCCCA GCACATTCGAGGTTACTATAAA >IE_08D07_translated MKSRREQSIEEAIVANYLKMMIDNVNVWPRHFLRSEDVYCKNPWTLFVTRDPIILHFGRYFFVNRSVNSGLTDGCEYGCWRIIGRDRVIKSVTTGKILGL KKVYKFCETDRKPKSVFKFLEKEKRRVRDRRIWAMEEYRFASTWKQDYVICKIRRLYPQPFDYMLAQHIRGYYK |
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| Alignment |
◆CDS（TAIRver.9）_without_stop_codonとのalignment◆ 522/525 (99.4%)（アミノ酸配列で174/175 (99.4%)）一致しました。
10 20 30 40 50 60 70 80 90 100
AT1G60 ATGATGAAGTCAAGACGTGAACAATCAATCGAAGAAGCAATCGTCGCAAATTATTTGAAGATGATGATCGATAACGTAAACGTTTGGCCTCGCCACTTCC
:::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_08D ---ATGAAGTCAAGACGTGAACAATCAATCGAAGAAGCAATCGTCGCAAATTATTTGAAGATGATGATCGATAACGTAAACGTTTGGCCTCGCCACTTCC
10 20 30 40 50 60 70 80 90
110 120 130 140 150 160 170 180 190 200
AT1G60 TCCGAAGCGAAGACGTGTACTGCAAGAATCCGTGGACGCTTTTCGTTACTAGAGATCCTATAATCCTACACTTCGGACGATACTTCTTCGTTAATCGGAG
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_08D TCCGAAGCGAAGACGTGTACTGCAAGAATCCGTGGACGCTTTTCGTTACTAGAGATCCTATAATCCTACACTTCGGACGATACTTCTTCGTTAATCGGAG
100 110 120 130 140 150 160 170 180 190
210 220 230 240 250 260 270 280 290 300
AT1G60 TGTGAATTCAGGTTTAACCGATGGATGTGAATACGGTTGTTGGAGAATCATCGGTCGTGATAGAGTGATCAAGTCGGTGACGACCGGGAAGATTCTAGGG
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_08D TGTGAATTCAGGTTTAACCGATGGATGTGAATACGGTTGTTGGAGAATCATCGGTCGTGATAGAGTGATCAAGTCGGTGACGACCGGGAAGATTCTAGGG
200 210 220 230 240 250 260 270 280 290
310 320 330 340 350 360 370 380 390 400
AT1G60 TTAAAGAAGGTTTATAAGTTCTGTGAAACTGATCGGAAACCGAAATCGGTTTTTAAGTTCTTGGAAAAGGAGAAGAGAAGAGTAAGAGATAGACGAATCT
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_08D TTAAAGAAGGTTTATAAGTTCTGTGAAACTGATCGGAAACCGAAATCGGTTTTTAAGTTCTTGGAAAAGGAGAAGAGAAGAGTAAGAGATAGACGAATCT
300 310 320 330 340 350 360 370 380 390
410 420 430 440 450 460 470 480 490 500
AT1G60 GGGCGATGGAAGAGTATAGGTTTGCAAGTACGTGGAAACAAGATTACGTGATCTGCAAGATTCGACGTCTGTATCCACAACCATTTGACTACATGTTGGC
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_08D GGGCGATGGAAGAGTATAGGTTTGCAAGTACGTGGAAACAAGATTACGTGATCTGCAAGATTCGACGTCTGTATCCACAACCATTTGACTACATGTTGGC
400 410 420 430 440 450 460 470 480 490
510 520
AT1G60 CCAGCACATTCGAGGTTACTATAAA
:::::::::::::::::::::::::
IE_08D CCAGCACATTCGAGGTTACTATAAA
500 510 520
10 20 30 40 50 60 70 80 90 100
AT1G60 MMKSRREQSIEEAIVANYLKMMIDNVNVWPRHFLRSEDVYCKNPWTLFVTRDPIILHFGRYFFVNRSVNSGLTDGCEYGCWRIIGRDRVIKSVTTGKILG
:::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_08D -MKSRREQSIEEAIVANYLKMMIDNVNVWPRHFLRSEDVYCKNPWTLFVTRDPIILHFGRYFFVNRSVNSGLTDGCEYGCWRIIGRDRVIKSVTTGKILG
10 20 30 40 50 60 70 80 90
110 120 130 140 150 160 170
AT1G60 LKKVYKFCETDRKPKSVFKFLEKEKRRVRDRRIWAMEEYRFASTWKQDYVICKIRRLYPQPFDYMLAQHIRGYYK
:::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_08D LKKVYKFCETDRKPKSVFKFLEKEKRRVRDRRIWAMEEYRFASTWKQDYVICKIRRLYPQPFDYMLAQHIRGYYK
100 110 120 130 140 150 160 170
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