◆◆◆IE_08D10（IE_09D03） detail◆◆◆

Code nameIE_08D10（IE_09D03）
LocusAT1G33280
TemplateIT_08D10 (CR634)
Donor VectorpDONR207
Forward primerIPF_08D10 (ggggacaagtttgtacaaaaaagcaggcttcATGAGTTCGTCTAACGGAGGTGTACCG)
Reverse primerIPR_08D10 (ggggaccactttgtacaagaaagctgggttAAACAACCCAAGAAATCCATTTTCACCGCG)
Alignment with template seq.1stアミノ酸配列99.70%一致2ndアミノ酸配列100%一致
Alignment with TAIR7CDSアミノ酸配列99.70%一致アミノ酸配列100%一致
comment1st:(Sequence解析まで行ったが、フレームはずれないが塩基置換がある)→2nd(09D03):100%
sequence 
>IE_08D10_915bp
ATGAGTTCGTCTAACGGAGGTGTACCGCCTGGTTTTCGGTTTCATCCAACGGACGAAGAACTTCTTCATTACTACTTGAAGAAGAAGATTTCTTATGAAA
AGTTTGAGATGGAAGTCATCAAAGAGGTTGATTTGAACAAGATTGAGCCGTGGGATTTACAAGATAGATGCAAAATAGGATCAACGCCACAAAATGAGTG
GTATTTCTTCAGCCACAAGGATAGGAAATATCCGACAGGGTCAAGGACAAACCGTGCAACCCACTCCGGTTTTTGGAAGGCGACAGGACGTGACAAATGC
ATAAGAAATTCTTACAAGAAGATCGGCATGAGGAAGACTCTTGTATTCTACAAAGGAAGAGCTCCTCATGGCCAAAAGACAGATTGGATCATGCACGAGT
ACCGTATCGAAGACACTGAAGATGACCCTTGTGAAGATGGATGGGTTGTTTGTAGAGTGTTCAAGAAGAAAAATCTGTTCAAAGTAGGGAATGATGTTGG
CTCAAACATCAGCAATAATAGGCTTGAAGCTCGTAGTTTCATCCGAAGAGAAAGCCCTTACCAAGGAATCTCAATGTTTGAGCTTAACAAGCCTGAAGAG
ATTAGTGTTCATCAATATCCTCAACCACCAATGTTTCAACCTCATCACAAGCCTCTTTCAATAGGCTATGACTATTCATTGGCTCTTCTACCAAGAGAAA
GCGAGTACCAACAAGCGTGTCAGCCATCAGGGGTTGAGGTTGGCACGTGCAAGGCAGTAAGTGAATGGGGAATAGTGAATTGTAACATGGTGAGTCATGA
GGACTCGTCTAGAGCGATGAGGTTTGAAGATGACGGTAACAACACTTCTTCCACGGTCCAGCCACCTTCTAATCTGCTTTCATTGCGCGGTGAAAATGGA
TTTCTTGGGTTGTTT

>IE_08D10_translated
MSSSNGGVPPGFRFHPTDEELLHYYLKKKISYEKFEMEVIKEVDLNKIEPWDLQDRCKIGSTPQNEWYFFSHKDRKYPTGSRTNRATHSGFWKATGRDKC
IRNSYKKIGMRKTLVFYKGRAPHGQKTDWIMHEYRIEDTEDDPCEDGWVVCRVFKKKNLFKVGNDVGSNISNNRLEARSFIRRESPYQGISMFELNKPEE
ISVHQYPQPPMFQPHHKPLSIGYDYSLALLPRESEYQQACQPSGVEVGTCKAVSEWGIVNCNMVSHEDSSRAMRFEDDGNNTSSTVQPPSNLLSLRGENG
FLGLF
Alignment ◆CDS（TAIRver.9）_without_stop_codonとのalignment◆

100%一致しました。

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