◆◆◆IE_08E01 detail◆◆◆

Code nameIE_08E01
LocusAT5G63790
TemplateIT_08E01 (CR641)
Donor VectorpDONR207
Forward primerIPF_08E01 (ggggacaagtttgtacaaaaaagcaggcttcATGGACTTTGCTCTCTTCTCCTCGATT)
Reverse primerIPR_08E01 (ggggaccactttgtacaagaaagctgggttCCCTTGAGGAGCAAAATTCCAATTCAAGAA)
Alignment with template seq.1stアミノ酸配列100%一致2nd-
Alignment with TAIR7CDSアミノ酸配列100%一致-
comment
sequence 
>IE_08E01_936bp
ATGGACTTTGCTCTCTTCTCCTCGATTTCAATCTTTGAGATAAACCACAAAGATCCTCCGATTCGAAGGTTTATAAAAACTCAAAATCGAATCTTATCCA
CAAGAAAACAACAAGGTACTTTTCCAAAAATGAAGGCGGAGTTGAATTTGCCGGCGGGATTCCGATTTCATCCGACGGACGAAGAGCTTGTCAAGTTCTA
TCTTTGCCGGAGATGTGCGTCAGAACCGATTAACGTTCCGGTTATCGCAGAGATTGACTTGTACAAATTCAATCCATGGGAGCTTCCAGAAATGGCGTTG
TACGGTGAGAAAGAATGGTACTTCTTCTCGCATAGAGACCGGAAATACCCAAACGGGTCGAGACCAAACCGGGCAGCTGGAACCGGTTATTGGAAAGCGA
CTGGAGCTGATAAACCGATCGGAAAACCGAAGACGTTAGGGATTAAGAAAGCACTCGTCTTCTACGCAGGAAAAGCTCCGAAAGGGATTAAAACGAATTG
GATTATGCACGAGTATCGTCTCGCTAATGTCGATCGATCTGCTTCTACCAACAAGAAGAACAACTTAAGACTTGATGATTGGGTTTTGTGTCGGATATAC
AATAAGAAAGGAACAATGGAGAAGTATTTACCGGCGGCGGCTGAGAAACCGACGGAAAAGATGAGTACGTCGGACTCAAGATGCTCAAGTCACGTGATTT
CACCGGACGTCACGTGTTCTGATAACTGGGAGGTTGAGAGTGAGCCCAAATGGATTAATCTGGAAGACGCGTTAGAGGCATTTAATGATGACACGTCCAT
GTTTAGTTCCATTGGTTTGTTGCAAAATGACGCCTTTGTTCCTCAGTTTCAGTACCAGTCCTCCGATTTCGTCGATTCGTTTCAGGACCCGTTCGAGCAG
AAACCGTTCTTGAATTGGAATTTTGCTCCTCAAGGG

>IE_08E01_translated
MDFALFSSISIFEINHKDPPIRRFIKTQNRILSTRKQQGTFPKMKAELNLPAGFRFHPTDEELVKFYLCRRCASEPINVPVIAEIDLYKFNPWELPEMAL
YGEKEWYFFSHRDRKYPNGSRPNRAAGTGYWKATGADKPIGKPKTLGIKKALVFYAGKAPKGIKTNWIMHEYRLANVDRSASTNKKNNLRLDDWVLCRIY
NKKGTMEKYLPAAAEKPTEKMSTSDSRCSSHVISPDVTCSDNWEVESEPKWINLEDALEAFNDDTSMFSSIGLLQNDAFVPQFQYQSSDFVDSFQDPFEQ
KPFLNWNFAPQG
Alignment ◆CDS（TAIRver.9）_without_stop_codonとのalignment◆

100%一致しました。

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