◆◆◆IE_08E02（IE_09D04） detail◆◆◆

Code nameIE_08E02（IE_09D04）
LocusAT5G35550
TemplateIT_08E02 (CR701)
Donor VectorpDONR207
Forward primerIPF_08E02 (ggggacaagtttgtacaaaaaagcaggcttcATGGGAAAGAGAGCAACTACTAGTGTG)
Reverse primerIPR_08E02 (ggggaccactttgtacaagaaagctgggttACAAGTGAAGTCTCGGAGCCAATCTTCATC)
Alignment with template seq.1stアミノ酸配列98.40%一致2ndアミノ酸配列100%一致
Alignment with TAIR7CDSアミノ酸配列98.40%一致アミノ酸配列100%一致
comment1st:(Sequence解析まで行ったが、フレームがずれる)→2nd(09D04):100%
sequence 
>IE_08E02_774bp
ATGGGAAAGAGAGCAACTACTAGTGTGAGGAGAGAAGAGTTAAACAGAGGAGCTTGGACTGATCATGAAGACAAGATCCTTAGAGATTACATCACCACTC
ACGGCGAAGGCAAATGGAGCACTCTCCCTAACCAAGCTGGTCTCAAGAGGTGTGGCAAAAGCTGTAGACTTCGGTGGAAGAACTACCTAAGACCGGGGAT
AAAGCGCGGTAACATCTCATCTGATGAAGAAGAACTCATAATCCGTCTCCATAATCTTCTTGGAAACAGATGGTCGTTGATAGCTGGGAGGCTTCCAGGC
CGAACAGACAATGAAATAAAGAATCATTGGAACTCAAACCTCCGCAAAAGACTTCCCAAAACTCAAACCAAGCAACCAAAACGTATAAAACATTCGACGA
ACAACGAGAATAATGTATGTGTTATACGTACAAAGGCGATTAGGTGCTCAAAGACTCTTCTCTTCTCGGATCTCTCTCTTCAGAAGAAGAGTAGTACTAG
TCCACTACCTCTGAAAGAACAAGAGATGGATCAAGGTGGATCTTCGTTGATGGGAGATCTCGAATTCGATTTCGATAGGATCCATTCGGAGTTTCACTTC
CCGGATTTGATGGATTTTGATGGTTTGGACTGTGGAAACGTTACATCTCTTGTTTCATCTAACGAGATTTTGGGAGAGTTGGTTCCTGCTCAAGGTAATC
TCGATCTCAATAGACCTTTCACTTCTTGTCATCATCGTGGCGACGATGAAGATTGGCTCCGAGACTTCACTTGT

>IE_08E02_translated
MGKRATTSVRREELNRGAWTDHEDKILRDYITTHGEGKWSTLPNQAGLKRCGKSCRLRWKNYLRPGIKRGNISSDEEELIIRLHNLLGNRWSLIAGRLPG
RTDNEIKNHWNSNLRKRLPKTQTKQPKRIKHSTNNENNVCVIRTKAIRCSKTLLFSDLSLQKKSSTSPLPLKEQEMDQGGSSLMGDLEFDFDRIHSEFHF
PDLMDFDGLDCGNVTSLVSSNEILGELVPAQGNLDLNRPFTSCHHRGDDEDWLRDFTC
Alignment ◆CDS（TAIRver.9）_without_stop_codonとのalignment◆

100%一致しました。

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