| Code name | IE_08E06 | |||
|---|---|---|---|---|
| Locus | AT2G17770 | |||
| Template | IT_08E06 (DR0109) | |||
| Donor Vector | pDONR207 | |||
| Forward primer | IPF_08E06 (ggggacaagtttgtacaaaaaagcaggcttcATGTTGTCATCAGCAAAGCATAATAAGA) | |||
| Reverse primer | IPR_08E06 (ggggaccactttgtacaagaaagctgggttAAATGGAGCTGTGGAAGACCGTTGTAGTGT) | |||
| Alignment with template seq. | 1st | アミノ酸配列100%一致 | 2nd | - |
| Alignment with TAIR7CDS | アミノ酸配列62.40%一致 | - | ||
| comment | 1st:Templateの配列とは一致 | |||
| sequence |
>IE_08E06_702bp ATGTTGTCATCAGCAAAGCATAATAAGATCAACAACCATAGTGCCTTTTCAATTTCCTCTTCATCATCATCATTATCAACATCATCCTCCCTAGGCCATA ACAAATCTCAAGTCACCATGGAAGAAGTATGGAAAGAAATCAACCTTGGTTCACTTCACTACCATCGGCAACTAAACATTGGTCATGAACCAATGTTAAA GAACCAAAACCCTAATAACTCCATCTTTCAAGATTTCCTCAACATGCCTCTGAATCAACCACCACCACCACCACCACCACCTTCCTCTTCCACCATTGTC ACTGCTCTCTATGGCTCTCTGCCTCTTCCGCCTCCTGCCACTGTCCTCAGCTTAAACTCCGGTGTTGGATTCGAGTTTCTTGATACCACAGAAAATCTTC TTGCTTCTAACCCTCGCTCCTTTGAGGAATCTGCAAAGTTTGGTTGTCTTGGTAAGAAAAGAGGCCAAGATTCTGATGATACTAGAGGAGACAGAAGGTA TAAGCGTATGATCAAGAACAGAGAATCTGCTGCTCGTTCAAGGGCTAGGAAGCAGGCATATACAAACGAACTTGAGCTTGAAATTGCTCACTTGCAGACA GAGAATGCAAGACTCAAGATACAACAAGAGCAGCTGAAAATAGCCGAAGCAACTCAAAACCAAGTAAAGAAAACACTACAACGGTCTTCCACAGCTCCAT TT >IE_08E06_translated MLSSAKHNKINNHSAFSISSSSSSLSTSSSLGHNKSQVTMEEVWKEINLGSLHYHRQLNIGHEPMLKNQNPNNSIFQDFLNMPLNQPPPPPPPPSSSTIV TALYGSLPLPPPATVLSLNSGVGFEFLDTTENLLASNPRSFEESAKFGCLGKKRGQDSDDTRGDRRYKRMIKNRESAARSRARKQAYTNELELEIAHLQT ENARLKIQQEQLKIAEATQNQVKKTLQRSSTAPF |
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| Alignment |
◆CDS（TAIRver.9）_without_stop_codonとのalignment◆ 457/707 (64.6%)（アミノ酸配列で149/234 (63.7%)）一致しました。
AT2G17 ----------------------------------------------------------------------------------------------------
IE_08E ATGTTGTCATCAGCAAAGCATAATAAGATCAACAACCATAGTGCCTTTTCAATTTCCTCTTCATCATCATCATTATCAACATCATCCTCCCTAGGCCATA
10 20 30 40 50 60 70 80 90 100
10 20 30 40 50 60 70 80
AT2G17 -----------------ATGGAAGAAGTATGGAAAGAAATCAACCTTGGTTCACTTCACTACCATCGGCAACTAAACATTGGTCATGAACCAATGTTAAA
:::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_08E ACAAATCTCAAGTCACCATGGAAGAAGTATGGAAAGAAATCAACCTTGGTTCACTTCACTACCATCGGCAACTAAACATTGGTCATGAACCAATGTTAAA
110 120 130 140 150 160 170 180 190 200
90 100 110 120 130 140 150 160 170 180
AT2G17 GAACCAAAACCCTAATAACTCCATCTTTCAAGATTTCCTCAACATGCCTCTGAATCAACCACCACCACCACCACCACCACCTTCCTCTTCCACCATTGTC
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_08E GAACCAAAACCCTAATAACTCCATCTTTCAAGATTTCCTCAACATGCCTCTGAATCAACCACCACCACCACCACCACCACCTTCCTCTTCCACCATTGTC
210 220 230 240 250 260 270 280 290 300
190 200 210 220 230 240 250 260 270 280
AT2G17 ACTGCTCTCTATGGCTCTCTGCCTCTTCCGCCTCCTGCCACTGTCCTCAGCTTAAACTCCGGTGTTGGATTCGAGTTTCTTGATACCACAGAAAATCTTC
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_08E ACTGCTCTCTATGGCTCTCTGCCTCTTCCGCCTCCTGCCACTGTCCTCAGCTTAAACTCCGGTGTTGGATTCGAGTTTCTTGATACCACAGAAAATCTTC
310 320 330 340 350 360 370 380 390 400
290 300 310 320 330 340 350 360 370 380
AT2G17 TTGCTTCTAACCCTCGCTCCTTTGAGGAATCTGCAAAGTTTGGTTGTCTTGGTAAGAAAAGAGGCCAAGATTCTGATGATACTAGAGGAGACAGAAGGTA
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_08E TTGCTTCTAACCCTCGCTCCTTTGAGGAATCTGCAAAGTTTGGTTGTCTTGGTAAGAAAAGAGGCCAAGATTCTGATGATACTAGAGGAGACAGAAGGTA
410 420 430 440 450 460 470 480 490 500
390 400 410 420 430 440 450 460
AT2G17 TAAGCGTATGATCAAGAACAGAGAATCTGCTGCTCGTTCAAGGGCTAGGAAGCAGGAATGTGTCTCTC---CTCACT----CTT-------CT-ACTTTC
:::::::::::::::::::::::::::::::::::::::::::::::::::::::: :: : : : ::: ::: :: :::: :
IE_08E TAAGCGTATGATCAAGAACAGAGAATCTGCTGCTCGTTCAAGGGCTAGGAAGCAGGCATAT-----ACAAACGAACTTGAGCTTGAAATTGCTCACTTGC
510 520 530 540 550 560 570 580 590
AT2G17 ----------------------------------------------------------------------------------------------------
IE_08E AGACAGAGAATGCAAGACTCAAGATACAACAAGAGCAGCTGAAAATAGCCGAAGCAACTCAAAACCAAGTAAAGAAAACACTACAACGGTCTTCCACAGC
600 610 620 630 640 650 660 670 680 690
AT2G17 -------
IE_08E TCCATTT
700
10 20 30 40 50 60
AT2G17 ---------------------------------------MEEVWKEINLGSLHYHRQLNIGHEPMLKNQNPNNSIFQDFLNMPLNQPPPPPPPPSSSTIV
:::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_08E MLSSAKHNKINNHSAFSISSSSSSLSTSSSLGHNKSQVTMEEVWKEINLGSLHYHRQLNIGHEPMLKNQNPNNSIFQDFLNMPLNQPPPPPPPPSSSTIV
10 20 30 40 50 60 70 80 90 100
70 80 90 100 110 120 130 140 150
AT2G17 TALYGSLPLPPPATVLSLNSGVGFEFLDTTENLLASNPRSFEESAKFGCLGKKRGQDSDDTRGDRRYKRMIKNRESAARSRARKQ------ECVSPHSST
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: : : :
IE_08E TALYGSLPLPPPATVLSLNSGVGFEFLDTTENLLASNPRSFEESAKFGCLGKKRGQDSDDTRGDRRYKRMIKNRESAARSRARKQAYTNELELEIAHLQT
110 120 130 140 150 160 170 180 190 200
AT2G17 F---------------------------------
IE_08E ENARLKIQQEQLKIAEATQNQVKKTLQRSSTAPF
210 220 230
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