◆◆◆RE_0443 detail◆◆◆

Code name	RE_0443
Locus	AT5G66730
Forward primer
Reverse primer
Alignment with TAIR7CDS	アミノ酸配列99.80%一致
comment	0520_20080115_0443_FRW1W2_5555
sequence	>RE_0443_1500bp ATGCCGGTTGATTTAGATAATTCCTCTACAGTTTCCGGCGATGCAAGCGTCTCATCGACCGGAAACCAAAATCTAACTCCTAAATCCGTCGGGAAGAAGA AACGGAATCTTCCTGGAATGCCCGATCCAGACGCTGAAGTGATAGCTTTGTCACCTAAAACACTTATGGCAACGAATCGATTCGTTTGCGAAATCTGTAA CAAAGGTTTTCAACGTGACCAGAATCTTCAGCTTCATCGTCGTGGTCACAATCTTCCATGGAAACTTCGACAGAGATCGACTAAAGAAGTGAGGAAGAAG GTTTATGTTTGTCCTGTTTCTGGCTGTGTTCATCATGACCCTTCACGTGCTCTTGGAGATCTTACTGGAATCAAGAAACACTTTTGTCGGAAACACGGCG AGAAGAAATGGAAGTGTGAGAAATGTTCTAAGAAGTATGCTGTTCAATCAGATTGGAAAGCTCATTCGAAGATTTGTGGCACCAAGGAGTATAAATGCGA TTGTGGAACTCTGTTTTCTAGGAGAGATAGCTTTATAACGCATAGAGCCTTTTGTGATGCCTTGGCTGAAGAGAGTGCGAAGAATCATACTCAAAGCAAG AAACTTTATCCAGAAACTGTCACTAGGAAGAATCCTGAAATTGAGCAAAAATCTCCAGCAGCTGTTGAGTCTTCTCCGTCATTACCGCCTTCTTCTCCGC CATCGGTTGCTATAGCTCCGGCACCGGCTATTTCGGTTGAGACTGAGTCTGTCAAAATCATATCTTCCTCTGTTTTGCCGATTCAGAATTCTCCAGAATC TCAAGAAAACAACAATCATCCTGAAGTCATTATTGAGGAAGCTTCAAGAACGATCGGGTTCAATGTTAGCTCATCGGATCTAAGCAATGATCACAGTAAC AACAATGGTGGATACGCAGGTTTGTTTGTATCATCAACAGCTTCGCCTAGTTTATATGCTTCCTCAACCGCTTCTCCAAGTCTATTCGCACCATCGTCCT CCATGGAACCCATCTCTCTCTGTCTCTCGACGAACCCTTCTTTGTTCGGACCAACAATACGAGATCCGCCACATTTCCTAACTCCTCTCCCTCCTCAACC GGCAATGTCAGCTACTGCATTGCTCCAAAAAGCTGCACAAATGGGTTCCACGGGATCAGGAGGTTCGTTGCTTCGCGGGTTAGGCATAGTTTCAACTACT TCTTCATCTATGGAACTCAGCAATCATGATGCATTGTCATTAGCTCCTGGTCTTGGACTTGGACTACCTTGTAGCAGCGGTGGTAGCGGATCGGGTCTAA AAGAGCTCATGATGGGGAATTCATCGGTGTTTGGTCCGAAACAGACGACACTAGACTTTCTTGGATTAGGAAGAGCTGTTGGTAATGGTGGTAATACCGG AGGTGGATTGTCTGCTCTGTTAACTTCTGTAGGTGGTGGAGGTGGAATCGATCTGTTTGGATCAGGGGAGTTTTCAGGCAAAGACATTGGAAGAAGTTCG >RE_0443_translated MPVDLDNSSTVSGDASVSSTGNQNLTPKSVGKKKRNLPGMPDPDAEVIALSPKTLMATNRFVCEICNKGFQRDQNLQLHRRGHNLPWKLRQRSTKEVRKK VYVCPVSGCVHHDPSRALGDLTGIKKHFCRKHGEKKWKCEKCSKKYAVQSDWKAHSKICGTKEYKCDCGTLFSRRDSFITHRAFCDALAEESAKNHTQSK KLYPETVTRKNPEIEQKSPAAVESSPSLPPSSPPSVAIAPAPAISVETESVKIISSSVLPIQNSPESQENNNHPEVIIEEASRTIGFNVSSSDLSNDHSN NNGGYAGLFVSSTASPSLYASSTASPSLFAPSSSMEPISLCLSTNPSLFGPTIRDPPHFLTPLPPQPAMSATALLQKAAQMGSTGSGGSLLRGLGIVSTT SSSMELSNHDALSLAPGLGLGLPCSSGGSGSGLKELMMGNSSVFGPKQTTLDFLGLGRAVGNGGNTGGGLSALLTSVGGGGGIDLFGSGEFSGKDIGRSS
Alignment	◆CDS（TAIRver.9）_without_stop_codonとのalignment◆ 1498/1500 (99.9%)（アミノ酸配列で499/500 (99.8%)）一致しました。 10 20 30 40 50 60 70 80 90 100 AT5G66 ATGCCGGTTGATTTAGATAATTCCTCTACAGTTTCCGGCGATGCAAGCGTCTCATCGACCGGAAACCAAAATCTAACTCCTAAATCCGTCGGGAAGAAGA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: RE_044 ATGCCGGTTGATTTAGATAATTCCTCTACAGTTTCCGGCGATGCAAGCGTCTCATCGACCGGAAACCAAAATCTAACTCCTAAATCCGTCGGGAAGAAGA 10 20 30 40 50 60 70 80 90 100 110 120 130 140 150 160 170 180 190 200 AT5G66 AACGGAATCTTCCTGGAATGCCTGATCCAGACGCTGAAGTGATAGCTTTGTCACCTAAAACACTTATGGCAACGAATCGATTCGTTTGCGAAATCTGTAA :::::::::::::::::::::: ::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: RE_044 AACGGAATCTTCCTGGAATGCCCGATCCAGACGCTGAAGTGATAGCTTTGTCACCTAAAACACTTATGGCAACGAATCGATTCGTTTGCGAAATCTGTAA 110 120 130 140 150 160 170 180 190 200 210 220 230 240 250 260 270 280 290 300 AT5G66 CAAAGGTTTTCAACGTGACCAGAATCTTCAGCTTCATCGTCGTGGTCACAATCTTCCATGGAAACTTCGACAGAGATCGACTAAAGAAGTGAGGAAGAAG :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: RE_044 CAAAGGTTTTCAACGTGACCAGAATCTTCAGCTTCATCGTCGTGGTCACAATCTTCCATGGAAACTTCGACAGAGATCGACTAAAGAAGTGAGGAAGAAG 210 220 230 240 250 260 270 280 290 300 310 320 330 340 350 360 370 380 390 400 AT5G66 GTTTATGTTTGTCCTGTTTCTGGCTGTGTTCATCATGACCCTTCACGTGCTCTTGGAGATCTTACTGGAATCAAGAAACACTTTTGTCGGAAACACGGCG :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: RE_044 GTTTATGTTTGTCCTGTTTCTGGCTGTGTTCATCATGACCCTTCACGTGCTCTTGGAGATCTTACTGGAATCAAGAAACACTTTTGTCGGAAACACGGCG 310 320 330 340 350 360 370 380 390 400 410 420 430 440 450 460 470 480 490 500 AT5G66 AGAAGAAATGGAAGTGTGAGAAATGTTCTAAGAAGTATGCTGTTCAATCAGATTGGAAAGCTCATTCGAAGATTTGTGGCACCAAGGAGTATAAATGCGA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: RE_044 AGAAGAAATGGAAGTGTGAGAAATGTTCTAAGAAGTATGCTGTTCAATCAGATTGGAAAGCTCATTCGAAGATTTGTGGCACCAAGGAGTATAAATGCGA 410 420 430 440 450 460 470 480 490 500 510 520 530 540 550 560 570 580 590 600 AT5G66 TTGTGGAACTCTGTTTTCTAGGAGAGATAGCTTTATAACGCATAGAGCCTTTTGTGATGCCTTGGCTGAAGAGAGTGCGAAGAATCATACTCAAAGCAAG :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: RE_044 TTGTGGAACTCTGTTTTCTAGGAGAGATAGCTTTATAACGCATAGAGCCTTTTGTGATGCCTTGGCTGAAGAGAGTGCGAAGAATCATACTCAAAGCAAG 510 520 530 540 550 560 570 580 590 600 610 620 630 640 650 660 670 680 690 700 AT5G66 AAACTTTATCCAGAAACTGTCACTAGGAAGAATCCTGAAATTGAGCAAAAATCTCCAGCAGCTGTTGAGTCTTCTCCGTCATTACCGCCTTCTTCTCCGC :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: RE_044 AAACTTTATCCAGAAACTGTCACTAGGAAGAATCCTGAAATTGAGCAAAAATCTCCAGCAGCTGTTGAGTCTTCTCCGTCATTACCGCCTTCTTCTCCGC 610 620 630 640 650 660 670 680 690 700 710 720 730 740 750 760 770 780 790 800 AT5G66 CATCGGTTGCTATAGCTCCGGCACCGGCTATTTCGGTTGAGACTGAGTCTGTCAAAATCATATCTTCCTCTGTTTTGCCGATTCAGAATTCTCCAGAATC :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: RE_044 CATCGGTTGCTATAGCTCCGGCACCGGCTATTTCGGTTGAGACTGAGTCTGTCAAAATCATATCTTCCTCTGTTTTGCCGATTCAGAATTCTCCAGAATC 710 720 730 740 750 760 770 780 790 800 810 820 830 840 850 860 870 880 890 900 AT5G66 TCAAGAAAACAACAATCATCCTGAAGTCATTATTGAGGAAGCTTCAAGAACGATCGGGTTCAATGTTAGCTCATCGGATCTAAGCAATGATCACAGTAAC :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: RE_044 TCAAGAAAACAACAATCATCCTGAAGTCATTATTGAGGAAGCTTCAAGAACGATCGGGTTCAATGTTAGCTCATCGGATCTAAGCAATGATCACAGTAAC 810 820 830 840 850 860 870 880 890 900 910 920 930 940 950 960 970 980 990 1000 AT5G66 AACAATGGTGGATACGCAGGTTTGTTTGTATCATCAACAGCTTCGCCTAGTTTATATGCTTCCTCAACCGCTTCTCCAAGTCTATTCGCACCATCGTCCT :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: RE_044 AACAATGGTGGATACGCAGGTTTGTTTGTATCATCAACAGCTTCGCCTAGTTTATATGCTTCCTCAACCGCTTCTCCAAGTCTATTCGCACCATCGTCCT 910 920 930 940 950 960 970 980 990 1000 1010 1020 1030 1040 1050 1060 1070 1080 1090 1100 AT5G66 CCATGGAACCCATCTCTCTCTGTCTCTCGACGAACCCTTCTTTGTTCGGACCAACAATACGAGATCCGCCACATTTCCTAACTCCTCTCCCTCCTCAACC :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: RE_044 CCATGGAACCCATCTCTCTCTGTCTCTCGACGAACCCTTCTTTGTTCGGACCAACAATACGAGATCCGCCACATTTCCTAACTCCTCTCCCTCCTCAACC 1010 1020 1030 1040 1050 1060 1070 1080 1090 1100 1110 1120 1130 1140 1150 1160 1170 1180 1190 1200 AT5G66 GGCAATGTCAGCTACTGCATTGCTCCAAAAAGCTGCACAAATGGGTTCCACGGGATCAGGAGGTTCGTTGCTTCGCGGGTTAGGCATAGTTTCAACTACT :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: RE_044 GGCAATGTCAGCTACTGCATTGCTCCAAAAAGCTGCACAAATGGGTTCCACGGGATCAGGAGGTTCGTTGCTTCGCGGGTTAGGCATAGTTTCAACTACT 1110 1120 1130 1140 1150 1160 1170 1180 1190 1200 1210 1220 1230 1240 1250 1260 1270 1280 1290 1300 AT5G66 TCTTCATCTATGGAACTCAGCAATCATGATGCATTGTCATTAGCTCCTGGTCTTGGACTTGGACTACCTTGTAGCAGCGGTGGTAGCGGATCGGGTCTAA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: RE_044 TCTTCATCTATGGAACTCAGCAATCATGATGCATTGTCATTAGCTCCTGGTCTTGGACTTGGACTACCTTGTAGCAGCGGTGGTAGCGGATCGGGTCTAA 1210 1220 1230 1240 1250 1260 1270 1280 1290 1300 1310 1320 1330 1340 1350 1360 1370 1380 1390 1400 AT5G66 AAGAGCTCATGATGGGGAATTCATCGGTGTTTGGTCCGAAACAGACGACACTAGACTTTCTTGGATTAGGAAGAGCTGTTGGTAATGGTGGTAATACCGG :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: RE_044 AAGAGCTCATGATGGGGAATTCATCGGTGTTTGGTCCGAAACAGACGACACTAGACTTTCTTGGATTAGGAAGAGCTGTTGGTAATGGTGGTAATACCGG 1310 1320 1330 1340 1350 1360 1370 1380 1390 1400 1410 1420 1430 1440 1450 1460 1470 1480 1490 1500 AT5G66 AGGTGGATTGTCTGCTCTGTTAACTTCTATAGGTGGTGGAGGTGGAATCGATCTGTTTGGATCAGGGGAGTTTTCAGGCAAAGACATTGGAAGAAGTTCG :::::::::::::::::::::::::::: ::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: RE_044 AGGTGGATTGTCTGCTCTGTTAACTTCTGTAGGTGGTGGAGGTGGAATCGATCTGTTTGGATCAGGGGAGTTTTCAGGCAAAGACATTGGAAGAAGTTCG 1410 1420 1430 1440 1450 1460 1470 1480 1490 1500 10 20 30 40 50 60 70 80 90 100 AT5G66 MPVDLDNSSTVSGDASVSSTGNQNLTPKSVGKKKRNLPGMPDPDAEVIALSPKTLMATNRFVCEICNKGFQRDQNLQLHRRGHNLPWKLRQRSTKEVRKK :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: RE_044 MPVDLDNSSTVSGDASVSSTGNQNLTPKSVGKKKRNLPGMPDPDAEVIALSPKTLMATNRFVCEICNKGFQRDQNLQLHRRGHNLPWKLRQRSTKEVRKK 10 20 30 40 50 60 70 80 90 100 110 120 130 140 150 160 170 180 190 200 AT5G66 VYVCPVSGCVHHDPSRALGDLTGIKKHFCRKHGEKKWKCEKCSKKYAVQSDWKAHSKICGTKEYKCDCGTLFSRRDSFITHRAFCDALAEESAKNHTQSK :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: RE_044 VYVCPVSGCVHHDPSRALGDLTGIKKHFCRKHGEKKWKCEKCSKKYAVQSDWKAHSKICGTKEYKCDCGTLFSRRDSFITHRAFCDALAEESAKNHTQSK 110 120 130 140 150 160 170 180 190 200 210 220 230 240 250 260 270 280 290 300 AT5G66 KLYPETVTRKNPEIEQKSPAAVESSPSLPPSSPPSVAIAPAPAISVETESVKIISSSVLPIQNSPESQENNNHPEVIIEEASRTIGFNVSSSDLSNDHSN :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: RE_044 KLYPETVTRKNPEIEQKSPAAVESSPSLPPSSPPSVAIAPAPAISVETESVKIISSSVLPIQNSPESQENNNHPEVIIEEASRTIGFNVSSSDLSNDHSN 210 220 230 240 250 260 270 280 290 300 310 320 330 340 350 360 370 380 390 400 AT5G66 NNGGYAGLFVSSTASPSLYASSTASPSLFAPSSSMEPISLCLSTNPSLFGPTIRDPPHFLTPLPPQPAMSATALLQKAAQMGSTGSGGSLLRGLGIVSTT :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: RE_044 NNGGYAGLFVSSTASPSLYASSTASPSLFAPSSSMEPISLCLSTNPSLFGPTIRDPPHFLTPLPPQPAMSATALLQKAAQMGSTGSGGSLLRGLGIVSTT 310 320 330 340 350 360 370 380 390 400 410 420 430 440 450 460 470 480 490 500 AT5G66 SSSMELSNHDALSLAPGLGLGLPCSSGGSGSGLKELMMGNSSVFGPKQTTLDFLGLGRAVGNGGNTGGGLSALLTSIGGGGGIDLFGSGEFSGKDIGRSS ::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::.::::::::::::::::::::::: RE_044 SSSMELSNHDALSLAPGLGLGLPCSSGGSGSGLKELMMGNSSVFGPKQTTLDFLGLGRAVGNGGNTGGGLSALLTSVGGGGGIDLFGSGEFSGKDIGRSS 410 420 430 440 450 460 470 480 490 500

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