◆◆◆RE_0515 detail◆◆◆

Code name	RE_0515
Locus	AT5G47140
Forward primer
Reverse primer
Alignment with TAIR7CDS	アミノ酸配列99.80%一致
comment	0428_アミノ酸配列70%以上一致
sequence	>RE_0515_1410bp ATGGGGAAGCAAGGTCCTTGCTATCACTGTGGAGTTACAAGTACACCTCTATGGAGAAACGGGCCACCAGAGAAGCCGGTGTTGTGCAATGCGTGTGGTT CGAGGTGGAGAACTAAAGGATCATTAGTAAACTACACACCTCTTCATGCTCGTGCTGAAGGTGATGAGACTGAGATTGAGGATCATAGAACTCAAACGGT GATGATTAAGGGAATGTCTTTGAACAAAAAGATTCCCAAGAGGAAACCATATCAAGAAAACTTCACAGTGAAAAGAGCTAACTTGGAATTCCATACCGGT TTCAAGAGGAAGGCTCTGGATGAAGAAGCTAGCAATAGATCGAGTTCAGGATCGGTTGTATCAAACTCCGAGAGCTGTGCACAATCTAATGCGTGGGACT CGACTTTTCCTTGTAAGAGAAGGACATGTGTGGGACGTCCAAAGGCAGCTTCTTCTGTTGAAGAGCTCACAAAGGATCTTTATACTATTCTACAAGAACA GCAATCTTCTTGTCTCTCTGGTACTTCAGAGGAAGATTTGCTTTTTGAGAATGAAACACCAATGCTGTTAGGACATGGTAGTGTTCTTATGAGAGATCCT CACTCAGGTGCTCGAGAAGAGGAATCTGAAGCTAGCTCACTCTTAGTTGAGAGCAGCAAGTCTTCATCAGTTCATTCTGTTAAATTTGGTGGAAAAGCAA TGAAGCAGGAGCAAGTGAAGAGGAGCAAATCTCAAGTCTTAGGAAGACATAGTTCACTACTCTGTAGCATAGATTTGAAGGATGTTTTCAACTTTGATGA GTTCATAGAAAATTTCACAGAGGAAGAACAGCAAAAACTGATGAAATTACTTCCTCAAGTTGACTCTGTTGATCGTCCTGATAGCCTCAGAAGCATGTTT GAGAGTTCTCAATTCAAAGAGAACTTATCCTTGTTTCAGCAACTTGTGGCAGATGGTGTTTTTGAGACAAATTCGTCTTATGCAAAACTTGAAGACATTA AGACACTTGCAAAGCTTGCTTTATCAGATCCTAACAAATCCCATTTGTTGGAAAGCTATTACATGCTCAAGAGAAGAGAGATTGAAGACTGTGTTACTAC AACATCAAGGGTCTCAAGCTTGAGTCCATCGAATAATAATAGTCTTGTAACCATTGAAAGACCTTGTGAAAGCTTAAACCAAAACTTCTCAGAGACAAGA GGTGTGATGAGAAGCCCGAAAGAAGTGATGAAGATTAGATCAAAGCACACCGAAGAGAATTTAGAGAATAGTGTATCTTCCTTTAAACCTGTGAGCTGTG GTGGACCTCTGGTGTTTAGCTATGAAGATAATGATATTTCTGATCAGGATCTTCTTCTTGATGTGCCGTCGAACGGCTCATTCCCTCAAGCAGAGCTTCT AAACATGATA >RE_0515_translated MGKQGPCYHCGVTSTPLWRNGPPEKPVLCNACGSRWRTKGSLVNYTPLHARAEGDETEIEDHRTQTVMIKGMSLNKKIPKRKPYQENFTVKRANLEFHTG FKRKALDEEASNRSSSGSVVSNSESCAQSNAWDSTFPCKRRTCVGRPKAASSVEELTKDLYTILQEQQSSCLSGTSEEDLLFENETPMLLGHGSVLMRDP HSGAREEESEASSLLVESSKSSSVHSVKFGGKAMKQEQVKRSKSQVLGRHSSLLCSIDLKDVFNFDEFIENFTEEEQQKLMKLLPQVDSVDRPDSLRSMF ESSQFKENLSLFQQLVADGVFETNSSYAKLEDIKTLAKLALSDPNKSHLLESYYMLKRREIEDCVTTTSRVSSLSPSNNNSLVTIERPCESLNQNFSETR GVMRSPKEVMKIRSKHTEENLENSVSSFKPVSCGGPLVFSYEDNDISDQDLLLDVPSNGSFPQAELLNMI
Alignment	◆CDS（TAIRver.9）_without_stop_codonとのalignment◆ 1409/1410 (99.9%)（アミノ酸配列で469/470 (99.8%)）一致しました。 10 20 30 40 50 60 70 80 90 100 AT5G47 ATGGGGAAGCAAGGTCCTTGCTATCACTGTGGAGTTACAAGTACACCTCTATGGAGAAACGGGCCACCAGAGAAGCCGGTGTTGTGCAATGCGTGTGGTT :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: RE_051 ATGGGGAAGCAAGGTCCTTGCTATCACTGTGGAGTTACAAGTACACCTCTATGGAGAAACGGGCCACCAGAGAAGCCGGTGTTGTGCAATGCGTGTGGTT 10 20 30 40 50 60 70 80 90 100 110 120 130 140 150 160 170 180 190 200 AT5G47 CGAGGTGGAGAACTAAAGGATCATTAGTAAACTACACACCTCTTCATGCTCGTGCTGAAGGTGATGAGACTGAGATTGAGGATCATAGAACTCAAACGGT :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: RE_051 CGAGGTGGAGAACTAAAGGATCATTAGTAAACTACACACCTCTTCATGCTCGTGCTGAAGGTGATGAGACTGAGATTGAGGATCATAGAACTCAAACGGT 110 120 130 140 150 160 170 180 190 200 210 220 230 240 250 260 270 280 290 300 AT5G47 GATGATTAAGGGAATGTCTTTGAACAAAAAGATTCCCAAGAGGAAACCATATCAAGAAAACTTCACAGTGAAAAGAGCTAACTTGGAATTCCATACCGGT :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: RE_051 GATGATTAAGGGAATGTCTTTGAACAAAAAGATTCCCAAGAGGAAACCATATCAAGAAAACTTCACAGTGAAAAGAGCTAACTTGGAATTCCATACCGGT 210 220 230 240 250 260 270 280 290 300 310 320 330 340 350 360 370 380 390 400 AT5G47 TTCAAGAGGAAGGCTCTGGATGAAGAAGCTAGCAATAGATCGAGTTCAGGATCGGTTGTATCAAACTCCGAGAGCTGTGCACAATCTAATGCGTGGGACT :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: RE_051 TTCAAGAGGAAGGCTCTGGATGAAGAAGCTAGCAATAGATCGAGTTCAGGATCGGTTGTATCAAACTCCGAGAGCTGTGCACAATCTAATGCGTGGGACT 310 320 330 340 350 360 370 380 390 400 410 420 430 440 450 460 470 480 490 500 AT5G47 CGACTTTTCCTTGTAAGAGAAGGACATGTGTGGGACGTCCAAAGGCAGCTTCTTCTGTTGAAAAGCTCACAAAGGATCTTTATACTATTCTACAAGAACA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: ::::::::::::::::::::::::::::::::::::: RE_051 CGACTTTTCCTTGTAAGAGAAGGACATGTGTGGGACGTCCAAAGGCAGCTTCTTCTGTTGAAGAGCTCACAAAGGATCTTTATACTATTCTACAAGAACA 410 420 430 440 450 460 470 480 490 500 510 520 530 540 550 560 570 580 590 600 AT5G47 GCAATCTTCTTGTCTCTCTGGTACTTCAGAGGAAGATTTGCTTTTTGAGAATGAAACACCAATGCTGTTAGGACATGGTAGTGTTCTTATGAGAGATCCT :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: RE_051 GCAATCTTCTTGTCTCTCTGGTACTTCAGAGGAAGATTTGCTTTTTGAGAATGAAACACCAATGCTGTTAGGACATGGTAGTGTTCTTATGAGAGATCCT 510 520 530 540 550 560 570 580 590 600 610 620 630 640 650 660 670 680 690 700 AT5G47 CACTCAGGTGCTCGAGAAGAGGAATCTGAAGCTAGCTCACTCTTAGTTGAGAGCAGCAAGTCTTCATCAGTTCATTCTGTTAAATTTGGTGGAAAAGCAA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: RE_051 CACTCAGGTGCTCGAGAAGAGGAATCTGAAGCTAGCTCACTCTTAGTTGAGAGCAGCAAGTCTTCATCAGTTCATTCTGTTAAATTTGGTGGAAAAGCAA 610 620 630 640 650 660 670 680 690 700 710 720 730 740 750 760 770 780 790 800 AT5G47 TGAAGCAGGAGCAAGTGAAGAGGAGCAAATCTCAAGTCTTAGGAAGACATAGTTCACTACTCTGTAGCATAGATTTGAAGGATGTTTTCAACTTTGATGA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: RE_051 TGAAGCAGGAGCAAGTGAAGAGGAGCAAATCTCAAGTCTTAGGAAGACATAGTTCACTACTCTGTAGCATAGATTTGAAGGATGTTTTCAACTTTGATGA 710 720 730 740 750 760 770 780 790 800 810 820 830 840 850 860 870 880 890 900 AT5G47 GTTCATAGAAAATTTCACAGAGGAAGAACAGCAAAAACTGATGAAATTACTTCCTCAAGTTGACTCTGTTGATCGTCCTGATAGCCTCAGAAGCATGTTT :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: RE_051 GTTCATAGAAAATTTCACAGAGGAAGAACAGCAAAAACTGATGAAATTACTTCCTCAAGTTGACTCTGTTGATCGTCCTGATAGCCTCAGAAGCATGTTT 810 820 830 840 850 860 870 880 890 900 910 920 930 940 950 960 970 980 990 1000 AT5G47 GAGAGTTCTCAATTCAAAGAGAACTTATCCTTGTTTCAGCAACTTGTGGCAGATGGTGTTTTTGAGACAAATTCGTCTTATGCAAAACTTGAAGACATTA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: RE_051 GAGAGTTCTCAATTCAAAGAGAACTTATCCTTGTTTCAGCAACTTGTGGCAGATGGTGTTTTTGAGACAAATTCGTCTTATGCAAAACTTGAAGACATTA 910 920 930 940 950 960 970 980 990 1000 1010 1020 1030 1040 1050 1060 1070 1080 1090 1100 AT5G47 AGACACTTGCAAAGCTTGCTTTATCAGATCCTAACAAATCCCATTTGTTGGAAAGCTATTACATGCTCAAGAGAAGAGAGATTGAAGACTGTGTTACTAC :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: RE_051 AGACACTTGCAAAGCTTGCTTTATCAGATCCTAACAAATCCCATTTGTTGGAAAGCTATTACATGCTCAAGAGAAGAGAGATTGAAGACTGTGTTACTAC 1010 1020 1030 1040 1050 1060 1070 1080 1090 1100 1110 1120 1130 1140 1150 1160 1170 1180 1190 1200 AT5G47 AACATCAAGGGTCTCAAGCTTGAGTCCATCGAATAATAATAGTCTTGTAACCATTGAAAGACCTTGTGAAAGCTTAAACCAAAACTTCTCAGAGACAAGA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: RE_051 AACATCAAGGGTCTCAAGCTTGAGTCCATCGAATAATAATAGTCTTGTAACCATTGAAAGACCTTGTGAAAGCTTAAACCAAAACTTCTCAGAGACAAGA 1110 1120 1130 1140 1150 1160 1170 1180 1190 1200 1210 1220 1230 1240 1250 1260 1270 1280 1290 1300 AT5G47 GGTGTGATGAGAAGCCCGAAAGAAGTGATGAAGATTAGATCAAAGCACACCGAAGAGAATTTAGAGAATAGTGTATCTTCCTTTAAACCTGTGAGCTGTG :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: RE_051 GGTGTGATGAGAAGCCCGAAAGAAGTGATGAAGATTAGATCAAAGCACACCGAAGAGAATTTAGAGAATAGTGTATCTTCCTTTAAACCTGTGAGCTGTG 1210 1220 1230 1240 1250 1260 1270 1280 1290 1300 1310 1320 1330 1340 1350 1360 1370 1380 1390 1400 AT5G47 GTGGACCTCTGGTGTTTAGCTATGAAGATAATGATATTTCTGATCAGGATCTTCTTCTTGATGTGCCGTCGAACGGCTCATTCCCTCAAGCAGAGCTTCT :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: RE_051 GTGGACCTCTGGTGTTTAGCTATGAAGATAATGATATTTCTGATCAGGATCTTCTTCTTGATGTGCCGTCGAACGGCTCATTCCCTCAAGCAGAGCTTCT 1310 1320 1330 1340 1350 1360 1370 1380 1390 1400 1410 AT5G47 AAACATGATA :::::::::: RE_051 AAACATGATA 1410 10 20 30 40 50 60 70 80 90 100 AT5G47 MGKQGPCYHCGVTSTPLWRNGPPEKPVLCNACGSRWRTKGSLVNYTPLHARAEGDETEIEDHRTQTVMIKGMSLNKKIPKRKPYQENFTVKRANLEFHTG :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: RE_051 MGKQGPCYHCGVTSTPLWRNGPPEKPVLCNACGSRWRTKGSLVNYTPLHARAEGDETEIEDHRTQTVMIKGMSLNKKIPKRKPYQENFTVKRANLEFHTG 10 20 30 40 50 60 70 80 90 100 110 120 130 140 150 160 170 180 190 200 AT5G47 FKRKALDEEASNRSSSGSVVSNSESCAQSNAWDSTFPCKRRTCVGRPKAASSVEKLTKDLYTILQEQQSSCLSGTSEEDLLFENETPMLLGHGSVLMRDP ::::::::::::::::::::::::::::::::::::::::::::::::::::::.::::::::::::::::::::::::::::::::::::::::::::: RE_051 FKRKALDEEASNRSSSGSVVSNSESCAQSNAWDSTFPCKRRTCVGRPKAASSVEELTKDLYTILQEQQSSCLSGTSEEDLLFENETPMLLGHGSVLMRDP 110 120 130 140 150 160 170 180 190 200 210 220 230 240 250 260 270 280 290 300 AT5G47 HSGAREEESEASSLLVESSKSSSVHSVKFGGKAMKQEQVKRSKSQVLGRHSSLLCSIDLKDVFNFDEFIENFTEEEQQKLMKLLPQVDSVDRPDSLRSMF :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: RE_051 HSGAREEESEASSLLVESSKSSSVHSVKFGGKAMKQEQVKRSKSQVLGRHSSLLCSIDLKDVFNFDEFIENFTEEEQQKLMKLLPQVDSVDRPDSLRSMF 210 220 230 240 250 260 270 280 290 300 310 320 330 340 350 360 370 380 390 400 AT5G47 ESSQFKENLSLFQQLVADGVFETNSSYAKLEDIKTLAKLALSDPNKSHLLESYYMLKRREIEDCVTTTSRVSSLSPSNNNSLVTIERPCESLNQNFSETR :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: RE_051 ESSQFKENLSLFQQLVADGVFETNSSYAKLEDIKTLAKLALSDPNKSHLLESYYMLKRREIEDCVTTTSRVSSLSPSNNNSLVTIERPCESLNQNFSETR 310 320 330 340 350 360 370 380 390 400 410 420 430 440 450 460 470 AT5G47 GVMRSPKEVMKIRSKHTEENLENSVSSFKPVSCGGPLVFSYEDNDISDQDLLLDVPSNGSFPQAELLNMI :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: RE_051 GVMRSPKEVMKIRSKHTEENLENSVSSFKPVSCGGPLVFSYEDNDISDQDLLLDVPSNGSFPQAELLNMI 410 420 430 440 450 460 470

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