◆◆◆RE_1195 detail◆◆◆

Code name	RE_1195
Locus	AT1G28520
Forward primer
Reverse primer
Alignment with TAIR7CDS	アミノ酸配列96.30%一致
comment	0526_20080115_1195_FRW1W2_2555
sequence	>RE_1195_1410bp ATGACGGGGAAGCGATCAAAGACTAATTGCAGATCTGCTTCTCACAAGCTCTTCAAGGATAAGGCTAAGAACCGTGTTGATGATTTGCAAGGCATGCTTT TGGATCTTCAGTTTGCGAGGAAAGAGAGTAGGCCTACTGATGTTACTCTTCTTGAGGAGCAAGTGAATCAGATGCTTCGTGAGTGGAAATCTGAGCTCAA TGAGCCTTCTCCAGCTTCATCCTTGCAACAACTCTGTGATGAGGAAGATGATGCTACCAGCAAATTAGCTGCCCCCAAGCCCGAGCCTGCTGATCAGAAT CTCGAAGCTGGCAAAGCTGCTGTCTTCCAAAGGGGATACAATTTGGTTCAGGGGAAGTCAGAACATGGATTACCATTGGTTGATAATTGCAAAGATTTGT CCTTAGCAGCTGGTAACAATTTCGATGGAACGGCTCCTTTGGAGTATCATCAGCAGTATGATCTGCAACAAGAGTTTGAACCAAACTTCAATGGTGGTTT CAACAATTGTCCCAGTTATGGTGTAGTAGAGGGTCCTATACATATCTCTAATTTTATCCCGACTATTTGTCCTCCACCCTCTGCATTCTTGGGTCCAAAA TGTGCTCTTTGGGATTGCCCTAGGCCAGCTCAGGGATTTGATTGGTTCCAGGATTACTGCAGCAGCTTCCACGCTGCACTGGCTTTCAATGAAGGGCCAC CAGGTATGAATCCGGTGGCGCGTCCTGGAGGTATCGGCCTAAAAGACGGTCTGCTTTTTGCTGCTCTTAGTGCAAAGGCTGGAGGGAAAGATGTTGGTAT TCCCGAATGTGAAGGAGCTGCAACTGCTAAATCTCCATGGAATGCTCCAGAGCTCTTTGATCTCACGGTTCTGGAGAGTGAGACACTAAGGGAGTGGCTA TTCTTTGACAAGCCAAGGAGGGCCTTTGAGAGCGGGAACAGAAAGCAAAGATCTTTACCAGACTACAATGGTCGTGGTTGGCACGAGTCACGTAAACAGA TCATTGTCGAGTTTGGAGGGCTGAAGAGATCTTACTACATGGATCCACAGCCTCTGCACCATTTCGAATGGCATCTTTACGAATATGAGATCAACAAGTG TGATGCTTGTGCCTTGTACAGGCTCGAGCTCAAGCTTGTTGACGGGAAGAAGACTTCAAAAGGCAAAGTCTCAAACGACTCAGTGGCTGATCTGCAGAAG CAGATGGGAAGACTCACAGCTGAGTTCCCTCCAGAAAACAATACCACTAACACCACCAACAACAACAAACGCTGCATCAAAGGAAGACCAAAAGTGAGCA CAAAAGTCGCCACCGGGAATGTTCAGAACACAGTAGAGCAGGCAAATGACTATGGAGTAGGTGAAGAGTTTAACTATCTGGTCGGAAATCTAAGCGACTA TTATATCCCC >RE_1195_translated MTGKRSKTNCRSASHKLFKDKAKNRVDDLQGMLLDLQFARKESRPTDVTLLEEQVNQMLREWKSELNEPSPASSLQQLCDEEDDATSKLAAPKPEPADQN LEAGKAAVFQRGYNLVQGKSEHGLPLVDNCKDLSLAAGNNFDGTAPLEYHQQYDLQQEFEPNFNGGFNNCPSYGVVEGPIHISNFIPTICPPPSAFLGPK CALWDCPRPAQGFDWFQDYCSSFHAALAFNEGPPGMNPVARPGGIGLKDGLLFAALSAKAGGKDVGIPECEGAATAKSPWNAPELFDLTVLESETLREWL FFDKPRRAFESGNRKQRSLPDYNGRGWHESRKQIIVEFGGLKRSYYMDPQPLHHFEWHLYEYEINKCDACALYRLELKLVDGKKTSKGKVSNDSVADLQK QMGRLTAEFPPENNTTNTTNNNKRCIKGRPKVSTKVATGNVQNTVEQANDYGVGEEFNYLVGNLSDYYIP
Alignment	◆CDS（TAIRver.9）_without_stop_codonとのalignment◆ 1408/1458 (96.6%)（アミノ酸配列で468/486 (96.3%)）一致しました。 10 20 30 40 50 60 70 80 90 100 AT1G28 ATGACGGGGAAGCGATCAAAGACTAATTGCAGATCTGCTTCTCACAAGCTCTTCAAGGATAAGGCTAAGAACCGTGTTGATGATTTGCAAGGCATGCTTT :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: RE_119 ATGACGGGGAAGCGATCAAAGACTAATTGCAGATCTGCTTCTCACAAGCTCTTCAAGGATAAGGCTAAGAACCGTGTTGATGATTTGCAAGGCATGCTTT 10 20 30 40 50 60 70 80 90 100 110 120 130 140 150 160 170 180 190 200 AT1G28 TGGATCTTCAGTTTGCGAGGAAAGAGAGTAGGCCTACTGATGTTACTCTTCTTGAGGAGCAAGTGAATCAGATGCTTCGTGAGTGGAAATCTGAGCTCAA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: RE_119 TGGATCTTCAGTTTGCGAGGAAAGAGAGTAGGCCTACTGATGTTACTCTTCTTGAGGAGCAAGTGAATCAGATGCTTCGTGAGTGGAAATCTGAGCTCAA 110 120 130 140 150 160 170 180 190 200 210 220 230 240 250 260 270 280 290 300 AT1G28 TGAGCCTTCTCCAGCTTCATCCTTGCAACAAGGTGGTACTCTGGGGTCTTTCTCATCAGATATTTGTCGGCTGCTGCAGCTCTGTGATGAGGAAGATGAT ::::::::::::::::::::::::::::::: ::::::::::::::::::::: RE_119 TGAGCCTTCTCCAGCTTCATCCTTGCAACAA------------------------------------------------CTCTGTGATGAGGAAGATGAT 210 220 230 240 250 310 320 330 340 350 360 370 380 390 400 AT1G28 GCTACCAGCAAATTAGCTGCCCCCAAGCCCGAGCCTGCTGATCAGAATCTCGAAGCTGGCAAAGCTGCTGTCTTCCAAAGGGGATACAATTTGGTTCAGG :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: RE_119 GCTACCAGCAAATTAGCTGCCCCCAAGCCCGAGCCTGCTGATCAGAATCTCGAAGCTGGCAAAGCTGCTGTCTTCCAAAGGGGATACAATTTGGTTCAGG 260 270 280 290 300 310 320 330 340 350 410 420 430 440 450 460 470 480 490 500 AT1G28 GGAAGTCAGAACATGGATTACCATTGGTTGATAATTGCAAAGATTTGTCCTTAGCAGCTGGTAACAATTTCGATGGAACGGCTCCTTTGGAGTATCATCA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: RE_119 GGAAGTCAGAACATGGATTACCATTGGTTGATAATTGCAAAGATTTGTCCTTAGCAGCTGGTAACAATTTCGATGGAACGGCTCCTTTGGAGTATCATCA 360 370 380 390 400 410 420 430 440 450 510 520 530 540 550 560 570 580 590 600 AT1G28 GCAGTATGATCTGCAACAAGAGTTTGAACCAAACTTCAATGGTGGTTTCAACAATTGTCCCAGTTATGGTGTAGTAGAGGGTCCTATACATATCTCTAAT :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: RE_119 GCAGTATGATCTGCAACAAGAGTTTGAACCAAACTTCAATGGTGGTTTCAACAATTGTCCCAGTTATGGTGTAGTAGAGGGTCCTATACATATCTCTAAT 460 470 480 490 500 510 520 530 540 550 610 620 630 640 650 660 670 680 690 700 AT1G28 TTTATCCCGACTATTTGTCCTCCACCCTCTGCATTCTTGGGTCCAAAATGTGCTCTTTGGGATTGCCCTAGGCCAGCTCAGGGATTTGATTGGTTCCAGG :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: RE_119 TTTATCCCGACTATTTGTCCTCCACCCTCTGCATTCTTGGGTCCAAAATGTGCTCTTTGGGATTGCCCTAGGCCAGCTCAGGGATTTGATTGGTTCCAGG 560 570 580 590 600 610 620 630 640 650 710 720 730 740 750 760 770 780 790 800 AT1G28 ATTACTGCAGCAGCTTCCACGCTGCACTGGCTTTCAATGAAGGGCCACCAGGTATGAATCCGGTGGTGCGTCCTGGAGGTATCGGCCTAAAAGACGGTCT :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: ::::::::::::::::::::::::::::::::: RE_119 ATTACTGCAGCAGCTTCCACGCTGCACTGGCTTTCAATGAAGGGCCACCAGGTATGAATCCGGTGGCGCGTCCTGGAGGTATCGGCCTAAAAGACGGTCT 660 670 680 690 700 710 720 730 740 750 810 820 830 840 850 860 870 880 890 900 AT1G28 GCTTTTTGCTGCTCTTAGTGCAAAGGCTGGAGGGAAAGATGTTGGTATTCCCGAATGTGAAGGAGCTGCAACTGCTAAATCTCCATGGAATGCTCCAGAG :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: RE_119 GCTTTTTGCTGCTCTTAGTGCAAAGGCTGGAGGGAAAGATGTTGGTATTCCCGAATGTGAAGGAGCTGCAACTGCTAAATCTCCATGGAATGCTCCAGAG 760 770 780 790 800 810 820 830 840 850 910 920 930 940 950 960 970 980 990 1000 AT1G28 CTCTTTGATCTCACGGTTCTGGAGAGTGAGACACTAAGGGAGTGGCTATTCTTTGACAAGCCAAGGAGGGCCTTTGAGAGCGGGAACAGAAAGCAAAGAT :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: RE_119 CTCTTTGATCTCACGGTTCTGGAGAGTGAGACACTAAGGGAGTGGCTATTCTTTGACAAGCCAAGGAGGGCCTTTGAGAGCGGGAACAGAAAGCAAAGAT 860 870 880 890 900 910 920 930 940 950 1010 1020 1030 1040 1050 1060 1070 1080 1090 1100 AT1G28 CTTTACCAGACTACAATGGTCGTGGTTGGCACGAGTCACGTAAACAGATCATGGTCGAGTTTGGAGGGCTGAAGAGATCTTACTACATGGATCCACAGCC :::::::::::::::::::::::::::::::::::::::::::::::::::: ::::::::::::::::::::::::::::::::::::::::::::::: RE_119 CTTTACCAGACTACAATGGTCGTGGTTGGCACGAGTCACGTAAACAGATCATTGTCGAGTTTGGAGGGCTGAAGAGATCTTACTACATGGATCCACAGCC 960 970 980 990 1000 1010 1020 1030 1040 1050 1110 1120 1130 1140 1150 1160 1170 1180 1190 1200 AT1G28 TCTGCACCATTTCGAATGGCATCTTTACGAATATGAGATCAACAAGTGTGATGCTTGTGCCTTGTACAGGCTCGAGCTCAAGCTTGTTGACGGGAAGAAG :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: RE_119 TCTGCACCATTTCGAATGGCATCTTTACGAATATGAGATCAACAAGTGTGATGCTTGTGCCTTGTACAGGCTCGAGCTCAAGCTTGTTGACGGGAAGAAG 1060 1070 1080 1090 1100 1110 1120 1130 1140 1150 1210 1220 1230 1240 1250 1260 1270 1280 1290 1300 AT1G28 ACTTCAAAAGGCAAAGTCTCAAACGACTCAGTGGCTGATCTGCAGAAGCAGATGGGAAGACTCACAGCTGAGTTCCCTCCAGAAAACAATACCACTAACA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: RE_119 ACTTCAAAAGGCAAAGTCTCAAACGACTCAGTGGCTGATCTGCAGAAGCAGATGGGAAGACTCACAGCTGAGTTCCCTCCAGAAAACAATACCACTAACA 1160 1170 1180 1190 1200 1210 1220 1230 1240 1250 1310 1320 1330 1340 1350 1360 1370 1380 1390 1400 AT1G28 CCACCAACAACAACAAACGCTGCATCAAAGGAAGACCAAAAGTGAGCACAAAAGTCGCCACCGGGAATGTTCAGAACACAGTAGAGCAGGCAAATGACTA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: RE_119 CCACCAACAACAACAAACGCTGCATCAAAGGAAGACCAAAAGTGAGCACAAAAGTCGCCACCGGGAATGTTCAGAACACAGTAGAGCAGGCAAATGACTA 1260 1270 1280 1290 1300 1310 1320 1330 1340 1350 1410 1420 1430 1440 1450 AT1G28 TGGAGTAGGTGAAGAGTTTAACTATCTGGTCGGAAATCTAAGCGACTATTATATCCCC :::::::::::::::::::::::::::::::::::::::::::::::::::::::::: RE_119 TGGAGTAGGTGAAGAGTTTAACTATCTGGTCGGAAATCTAAGCGACTATTATATCCCC 1360 1370 1380 1390 1400 1410 10 20 30 40 50 60 70 80 90 100 AT1G28 MTGKRSKTNCRSASHKLFKDKAKNRVDDLQGMLLDLQFARKESRPTDVTLLEEQVNQMLREWKSELNEPSPASSLQQGGTLGSFSSDICRLLQLCDEEDD :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: :::::::: RE_119 MTGKRSKTNCRSASHKLFKDKAKNRVDDLQGMLLDLQFARKESRPTDVTLLEEQVNQMLREWKSELNEPSPASSLQ----------------QLCDEEDD 10 20 30 40 50 60 70 80 110 120 130 140 150 160 170 180 190 200 AT1G28 ATSKLAAPKPEPADQNLEAGKAAVFQRGYNLVQGKSEHGLPLVDNCKDLSLAAGNNFDGTAPLEYHQQYDLQQEFEPNFNGGFNNCPSYGVVEGPIHISN :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: RE_119 ATSKLAAPKPEPADQNLEAGKAAVFQRGYNLVQGKSEHGLPLVDNCKDLSLAAGNNFDGTAPLEYHQQYDLQQEFEPNFNGGFNNCPSYGVVEGPIHISN 90 100 110 120 130 140 150 160 170 180 210 220 230 240 250 260 270 280 290 300 AT1G28 FIPTICPPPSAFLGPKCALWDCPRPAQGFDWFQDYCSSFHAALAFNEGPPGMNPVVRPGGIGLKDGLLFAALSAKAGGKDVGIPECEGAATAKSPWNAPE ::::::::::::::::::::::::::::::::::::::::::::::::::::::: :::::::::::::::::::::::::::::::::::::::::::: RE_119 FIPTICPPPSAFLGPKCALWDCPRPAQGFDWFQDYCSSFHAALAFNEGPPGMNPVARPGGIGLKDGLLFAALSAKAGGKDVGIPECEGAATAKSPWNAPE 190 200 210 220 230 240 250 260 270 280 310 320 330 340 350 360 370 380 390 400 AT1G28 LFDLTVLESETLREWLFFDKPRRAFESGNRKQRSLPDYNGRGWHESRKQIMVEFGGLKRSYYMDPQPLHHFEWHLYEYEINKCDACALYRLELKLVDGKK ::::::::::::::::::::::::::::::::::::::::::::::::::.::::::::::::::::::::::::::::::::::::::::::::::::: RE_119 LFDLTVLESETLREWLFFDKPRRAFESGNRKQRSLPDYNGRGWHESRKQIIVEFGGLKRSYYMDPQPLHHFEWHLYEYEINKCDACALYRLELKLVDGKK 290 300 310 320 330 340 350 360 370 380 410 420 430 440 450 460 470 480 AT1G28 TSKGKVSNDSVADLQKQMGRLTAEFPPENNTTNTTNNNKRCIKGRPKVSTKVATGNVQNTVEQANDYGVGEEFNYLVGNLSDYYIP :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: RE_119 TSKGKVSNDSVADLQKQMGRLTAEFPPENNTTNTTNNNKRCIKGRPKVSTKVATGNVQNTVEQANDYGVGEEFNYLVGNLSDYYIP 390 400 410 420 430 440 450 460 470

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