|
|
|
|
|
|
|
|
| Locus | Common name | Short description |
| AT1G25470 | CRF12 | cytokinin response factor 12, AP2 domain-containing transcription factor family protein (.1.2) |
>AT1G25470.2 CDS map
>>Show genomic map
Uncut enzymes: Acc65I, AccI, AccII(FnuDII), AccIII(BspMII), AclI, AfeI, AflII, AflIII, AgeI, AhdI, AlwI, Aor51HI(Eco47III), ApaI, ApaLI, AscI, AseI, AvaI, AvaII, AvrII, BaeI, BalI, BamHI, BanI, BanII, BbsI, BbvCI, BbvI, BcgI, BciVI, BclI, BglI, BglII, BlpI, BmrI, BpmI, BsaAI, BsaBI, BsaI, BsaWI, BsaXI, BseRI, BsgI, BsiEI, BsiHKAI, BsiWI, BsmAI, BsmBI, BsmFI, BsmI, BsoBI, Bsp1286I, BspDI, BspEI, BspMI, BspT107I(HgiCI), BsrBI, BsrFI, BsrGI, BssHII, BssKI, BssSI, BstBI, BstNI, BstUI, BstXI, BstYI, BstZ17I, BtgI, BtrI, BtsI, Cfr10I, ClaI, DraI, DraIII, DrdI, EaeI, EagI, EarI, EciI, EcoO109I, FauI, FokI, FseI, FspI, HaeII, HgaI, HhaI, HinP1I, HindIII, HpaI, HpaII, HphI, Hpy99I, I-CeuI, I-SceI, KasI, KpnI, MboII, MfeI, MluI, MlyI, MnlI, MscI, MspA1I, MspI, N.BstNBI, NaeI, , NarI, NciI, NcoI, NdeI, NgoMIV, NheI, NlaIV, NotI, NruI, NsiI, NspI, PI-PspI, PI-SceI(VDE), PacI, PaeR7I, PciI, PflFI, PflMI, PleI, PmeI, PmlI, PpuMI, PshAI, PsiI, PspGI, PspOMI, PvuI, PvuII, RsrII, SacI, SacII, SalI, SapI, Sau96I, SbfI, ScaI, ScrFI, SexAI, SfaNI, SfiI, SfoI, SgrAI, SmaI, SmlI, SnaBI, SpeI, SphI, SspI, StuI, StyI, SwaI, TliI, Tth111I, XbaI, XcmI, XhoI, XmaI
| Name | Recognition sequence | Cut positions |
| AatII | GACGT|C | 507 |
| AciI | C|CGC | 842 |
| AluI | AG|CT | 343, 654, 762, 798 |
| AlwNI | CAGNNN|CTG | 344, 401 |
| ApoI | Pu|AATTPy | 271, 793, 814 |
| BfaI | C|TAG | 93, 207, 222, 432, 656 |
| BsaHI | GPu|CGPyC | 507 |
| BsaJI | C|CNNGG | 256 |
| BslI | CCNNNNN|NNGG | 161, 211, 849 |
| BspHI | T|CATGA | 141 |
| BsrDI | GCAATGNN| | 193 |
| BsrI | ACTGGN| | 387 |
| BstAPI | GCANNNN|NTGC | 648 |
| BstEII | G|GTNACC | 108 |
| BstF5I | GGATGNN| | 583, 700 |
| Bsu36I | CC|TNAGG | 161 |
| Cac8I | GCN|NGC | 443 |
| DdeI | C|TNAG | 19, 160, 178, 280, 307, 346, 600, 608 |
| DpnI | GA-CH3|TC | 62, 182, 596, 827 |
| DpnII | |GATC | 62, 182, 596, 827 |
| EcoNI | CCTNN|NNNAGG | 161 |
| EcoRI | G|AATTC | 793 |
| EcoRV | GAT|ATC | 724 |
| Fnu4HI | GC|NGC | 276, 528, 612 |
| HaeIII | GG|CC | 252, 258 |
| HincII | GTPy|PuAC | 361 |
| HinfI | G|ANTC | 27, 81, 540, 604, 624, 681 |
| Hpy188I | TCN|GA | 168, 512, 579, 599, 833 |
| HpyCH4III | ACN|GT | 42, 426, 748, 772 |
| HpyCH4IV | A|CGT | 506 |
| HpyCH4V | TG|CA | 337, 382, 640, 649 |
| MboI | |GATC | 62, 182, 596, 827 |
| MseI | T|TAA | 231, 859 |
| MslI | CAPyNN|NNPuTG | 787 |
| MwoI | GCNNNNN|NNGC | 351, 648 |
| NlaIII | CATG| | 140 |
| PstI | CTGCA|G | 338 |
| RsaI | GT|AC | 321 |
| Sau3AI | |GATC | 62, 182, 596, 827 |
| SfcI | C|TPuPyAG | 338 |
| Taqƒ¿I | T|CGA | 118, 570, 714 |
| TfiI | G|A[AT]TC | 81, 540, 604, 624 |
| TseI | G|C[AT]GC | 276, 528, 612 |
| Tsp45I | |GT[CG]AC | 429 |
| Tsp509I | |AATT | 270, 792, 813 |
| TspRI | NNCA[CG]TGNN| | 61, 387, 429, 440, 784 |
| XmnI | GAANN|NNTTC | 295 |
Click here to view TAIR data
>AT1G25470.2 length:864
ATGAAGTCCT TTGTGAAACC TGAGAGAGAC TCTTTGTTGA GAACAGTTCG | 50 |
TATTGTTTTC ACTGATCCTG ATGCTACTGA TGATTCCTCT TCCTCTAGCG | 100 |
ATGAATGGTT ACCTAAACCT CGAAAGGTAA AGCGTTTTGT TCATGAGATT | 150 |
ACTTTCCTCC CTCAGGTCTC CGAGAGTTCT CAGGATCGTA GCAATGCTGT | 200 |
CAAAACCCCT AGAAGGAAAT CTACTAGGCA GTTTAAGTAT CCTGTGGGAG | 250 |
TTAGGCCGAG GCCATCAGGC AAATTTGCTG CTGAGATTTT GAACCCATTC | 300 |
ACTAAAACTA AGAAATGGTT GGGTACTTAT GAAACTCCTG CAGAAGCTGA | 350 |
GAAGGCTTAT GTTGACAAGA AGGTTGAGTA TGATGCACTG GCTTCTTCAG | 400 |
GTTCTGCTGT TTCCTCATCG GTTGTAACAG TGACTAGCCA GTGCTTGCGT | 450 |
TCCCCTACTT CTGCTTCTGT TTCTTGTGTA AGTGCTGATG ATTTGTCTAA | 500 |
AGAAAAGACG TCTCTGAACA AAGATGTTGC TGCGTCTGGT GATTCAACCA | 550 |
CCAAAGAAGT GTTCACTACT TTCGACTTTT CGGATGTGAA AATACCTGAT | 600 |
CTGAGATTCT TAGCAGCAGA AGAGGATTCT ATGGTTTCTA ATGCAAATGG | 650 |
TGCAGAGCTA GACTTTGATT GTTTCCTTAC TGACTCTAAC ATTCTGCTGG | 700 |
ATGATTACTC ATTGCTCGAA AATGATATCA ACTTCAGCAG GTTTGAAAAC | 750 |
AGTCTTCCAA GTGAGCTACC TGACTGTGAT TTCACTGAAA TGGAATTCCA | 800 |
GCTTGATGAT TTCAAATTTG CCTATACAGA TCATCTGACA ACACCGCCTC | 850 |
TCGGTTTGGT TTAA | 900 |
>AT1G25470.2 longest ORF length:287
MKSFVKPERD SLLRTVRIVF TDPDATDDSS SSSDEWLPKP RKVKRFVHEI | 50 |
TFLPQVSESS QDRSNAVKTP RRKSTRQFKY PVGVRPRPSG KFAAEILNPF | 100 |
TKTKKWLGTY ETPAEAEKAY VDKKVEYDAL ASSGSAVSSS VVTVTSQCLR | 150 |
SPTSASVSCV SADDLSKEKT SLNKDVAASG DSTTKEVFTT FDFSDVKIPD | 200 |
LRFLAAEEDS MVSNANGAEL DFDCFLTDSN ILLDDYSLLE NDINFSRFEN | 250 |
SLPSELPDCD FTEMEFQLDD FKFAYTDHLT TPPLGLV | 300 |