fioreDB : Database for Flower Bio-engineering by CRES-T system
fioreDB : Database for Flower Bio-engineering by CRES-T system

>>AT2G47460 (PFG1, ATMYB12)

Alias PFG1, ATMYB12
TF Classification
FioreDBMYB (Click to show phylogenetic tree)
RARTFMYB superfamily / GL1
AtTFDBMYB / R2R3-MYBs
PlnTFDBMYB (v3.0), MYB (v1.0)
DATFMYB
TAIR short description PRODUCTION OF FLAVONOL GLYCOSIDES 1, myb domain protein 12 (.1)
TAIR annotation "MYB12 belongs to subgroup 7 of the R2R3-MYB family. It strongly activates the promoters of chalcone synthase (CHS), flavanone 3-hydroxylase (F3H), flavonol synthase (FLS) and - to a lesser extent - chalcone flavanone isomerase (CHI), but cannot activate the promoters of flavonoid-3'hydroxylase (F3'H) and dihydroflavonol 4-reductase (DF). The activation requires a functional MYB recognition element (MRE). Results from the myb12-1f allele indicate that an activation domain might be present in the C-terminus. Overexpression or knock-out plants do not show any obvious phenotype under greenhouse conditions. Young myb12-ko seedlings contain reduced amounts of flavonoids (quercetin and kaempferol), while seedlings as well as leaves of MYB12-OX plants displayed an increased flavonoid content. They did not show any significant difference in anthocyanin content. Expression of CHS and FLS shows a clear correlation to MYB12 expression levels. CHI and F3H show increased transcript levels in the MYB12-OX lines, but no differences in the knock-out. Even in the absence of functional MYB12, flavonol biosynthesis is not completely absent, suggesting functional redundancy. " The redundant factors are MYB11 and MYB111 although MYB12 is primarily required for flavonol biosynthesis in roots. (.1)
Gene model
Gene model cDNA support
AT2G47460.1 18
Entry clone (w/o stop) RE_1381_x
External link
General info TAIR DATA
Genomic seq TAIR seq viewer
SNP SALK SNP Viewer
T-DNA tag line T-DNA Express
miRNA Search ASRP
Phylogeny SALAD database
Promoter ppdb
Microarray data
FioreDB
ATTED-II ATTED-II data
NCBI GEO NCBI GEO profile
NASC NASCarray_SpotHistory NASCarray_DigitalNorthern
Genevestigator Genevestigator
eFP Browser Developmental Map Abiotic Stress
AtGenExpress Development Hormone Abiotic Stress Light Pathogen
Gene Ontology (GO)
(P) 10224: response to abiotic or biotic stimulus ->response to UV-B
(F) 1135: other molecular functions ->transcription factor activity, RNA polymerase II transcription factor recruiting
(P) 1900386: other metabolic processes ->positive regulation of flavonol biosynthetic process
(P) 30154: other cellular processes ->cell differentiation
(F) 3677: DNA or RNA binding ->DNA binding
(F) 3700: transcription factor activity ->transcription factor activity, sequence-specific DNA binding
(F) 43565: DNA or RNA binding ->sequence-specific DNA binding
(F) 44212: nucleic acid binding ->transcription regulatory region DNA binding
(P) 45893: other cellular processes ->positive regulation of transcription, DNA-templated
(P) 51555: other metabolic processes ->flavonol biosynthetic process
(F) 5515: protein binding ->protein binding
(C) 5634: nucleus ->nucleus
(P) 6351: transcription,DNA-dependent ->transcription, DNA-templated
(P) 6355: other metabolic processes ->regulation of transcription, DNA-templated
(P) 6357: transcription,DNA-dependent ->regulation of transcription from RNA polymerase II promoter
(P) 9416: response to abiotic or biotic stimulus ->response to light stimulus
(P) 9651: response to stress ->response to salt stress
(P) 9723: other biological processes ->response to ethylene
(P) 9733: other biological processes ->response to auxin
(F) 981: transcription factor activity ->RNA polymerase II transcription factor activity, sequence-specific DNA binding
(P) 9813: other metabolic processes ->flavonoid biosynthetic process
miRNA/tasiRNA
Target sequence miRNA/tasiRNA sequence Map
5'--3'

miR858: 3'-TTCCAGCTTGTCTGTTGCTTT-5'
Orthologs
Type
Ia Ib II III Total
poplar
soybean 1 1
rice 1 1
sorghum 1 1
physcomitrella
Total 3 3
Repression motif 

       

       

        Repression motif
in putative orthologs		
        
        

       

       

        Status
        
         

          
Plasmid construction -> done

          
Plasmid construction -> done

          
Transformed -> done

          
T1 seed harvested -> done

          
T2 seed harvested -> done

         

        		
       

       

        CRES-T phenotype from individual project
        
        

        		
       

       

        CRES-T phenotype from publication
        

        
       


       

        CRES-T phenotype from bulk project
        

        
       

       

        Phenotype in ornamental plants


        
         

          Comment