fioreDB : Database for Flower Bio-engineering by CRES-T system
fioreDB : Database for Flower Bio-engineering by CRES-T system

>>AT1G59750 (ARF1)

Alias ARF1
TF Classification
FioreDBARF (Click to show phylogenetic tree)
RARTFARF / MONOPTEROS/ARF5
AtTFDBARF
PlnTFDBARF (v3.0), ARF (v1.0)
DATFARF
TAIR short description auxin response factor 1 (.1.2.3.4)
TAIR annotation Encodes a member of the auxin response factor family. ARFs bind to the cis element 5'-TGTCTC-3' ARFs mediate changes in gene expression in response to auxin. ARF's form heterodimers with IAA/AUX genes. ARF1 enhances mutant phenotypes of ARF2 and may act with ARF2 to control aspects of maturation and senescence.ARF1:LUC and 3xHA:ARF1 proteins have a half-life of ~3-4 hours and their degradation is reduced by proteasome inhibitors. 3xHA:ARF1 degradation is not affected by a pre-treatment with IAA. A nuclear-targeted fusion protein containing the middle region of ARF1 linked to LUC:NLS has a similar half-life to the full-length ARF1:LUC construct. The degradation of 3xHA:ARF1 is not affected in an axr6-3 mutant grown at room temperature, although the degradation of AXR2/IAA7 is slowed under these conditions. (.1), Encodes a member of the auxin response factor family. ARFs bind to the cis element 5'-TGTCTC-3' ARFs mediate changes in gene expression in response to auxin. ARF's form heterodimers with IAA/AUX genes. ARF1 enhances mutant phenotypes of ARF2 and may act with ARF2 to control aspects of maturation and senescence.ARF1:LUC and 3xHA:ARF1 proteins have a half-life of ~3-4 hours and their degradation is reduced by proteasome inhibitors. 3xHA:ARF1 degradation is not affected by a pre-treatment with IAA. A nuclear-targeted fusion protein containing the middle region of ARF1 linked to LUC:NLS has a similar half-life to the full-length ARF1:LUC construct. The degradation of 3xHA:ARF1 is not affected in an axr6-3 mutant grown at room temperature, although the degradation of AXR2/IAA7 is slowed under these conditions. (.2), Encodes a member of the auxin response factor family. ARFs bind to the cis element 5'-TGTCTC-3' ARFs mediate changes in gene expression in response to auxin. ARF's form heterodimers with IAA/AUX genes. ARF1 enhances mutant phenotypes of ARF2 and may act with ARF2 to control aspects of maturation and senescence.ARF1:LUC and 3xHA:ARF1 proteins have a half-life of ~3-4 hours and their degradation is reduced by proteasome inhibitors. 3xHA:ARF1 degradation is not affected by a pre-treatment with IAA. A nuclear-targeted fusion protein containing the middle region of ARF1 linked to LUC:NLS has a similar half-life to the full-length ARF1:LUC construct. The degradation of 3xHA:ARF1 is not affected in an axr6-3 mutant grown at room temperature, although the degradation of AXR2/IAA7 is slowed under these conditions. (.3), Encodes a member of the auxin response factor family. ARFs bind to the cis element 5'-TGTCTC-3' ARFs mediate changes in gene expression in response to auxin. ARF's form heterodimers with IAA/AUX genes. ARF1 enhances mutant phenotypes of ARF2 and may act with ARF2 to control aspects of maturation and senescence.ARF1:LUC and 3xHA:ARF1 proteins have a half-life of ~3-4 hours and their degradation is reduced by proteasome inhibitors. 3xHA:ARF1 degradation is not affected by a pre-treatment with IAA. A nuclear-targeted fusion protein containing the middle region of ARF1 linked to LUC:NLS has a similar half-life to the full-length ARF1:LUC construct. The degradation of 3xHA:ARF1 is not affected in an axr6-3 mutant grown at room temperature, although the degradation of AXR2/IAA7 is slowed under these conditions. (.4)
Gene model
Gene model cDNA support
AT1G59750.1 60
AT1G59750.2 58
AT1G59750.4 57
AT1G59750.3 53
Entry clone (w/o stop) RE_0159
External link
General info TAIR DATA
Genomic seq TAIR seq viewer
SNP SALK SNP Viewer
T-DNA tag line T-DNA Express
miRNA Search ASRP
Phylogeny SALAD database
Promoter ppdb
Microarray data
FioreDB
ATTED-II ATTED-II data
NCBI GEO NCBI GEO profile
NASC NASCarray_SpotHistory NASCarray_DigitalNorthern
Genevestigator Genevestigator
eFP Browser Developmental Map Abiotic Stress
AtGenExpress Development Hormone Abiotic Stress Light Pathogen
Gene Ontology (GO)
(P) 10150: developmental processes ->leaf senescence
(F) 3677: DNA or RNA binding ->DNA binding
(F) 3700: transcription factor activity ->transcription factor activity, sequence-specific DNA binding
(F) 42802: protein binding ->identical protein binding
(P) 45892: transcription,DNA-dependent ->negative regulation of transcription, DNA-templated
(F) 5515: protein binding ->protein binding
(C) 5634: nucleus ->nucleus
(P) 6351: transcription,DNA-dependent ->transcription, DNA-templated
(P) 6355: transcription,DNA-dependent ->regulation of transcription, DNA-templated
(P) 9733: other biological processes ->response to auxin
(P) 9734: signal transduction ->auxin-activated signaling pathway
miRNA/tasiRNA
Orthologs
Type
Ia Ib II III Total
poplar 1 9 10
soybean 1 12 13
rice 1 1 2
sorghum 1 1 2
physcomitrella
Total 4 23 27
Repression motif
Repression motif
in putative orthologs
SGVPSSLSLNLLDSNT at 556/802 in soybean Gm013594 (Type III)
SGVPSSLSLNLLDSNT at 556/858 in soybean Gm013592 (Type III)
PGVPSSLSLNLLDSNL at 549/851 in soybean Gm019793 (Type III)
SGVPSSLSLNLLDSNT at 556/858 in soybean Gm013593 (Type III)
SIMSPGLSLKLSESNS at 555/854 in poplar Pt023628 (Type III)

        
Status
Plasmid construction -> done
Plasmid construction -> done
Transformed -> done
T1 seed harvested -> done
T2 seed harvested -> done
CRES-T phenotype from individual project
tall height (direct input), too large leaf (direct input)
CRES-T phenotype from publication
CRES-T phenotype from bulk project short stamen, sterile or low fertility (A20274-144)

Phenotype in ornamental plants

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