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fioreDB : Database for Flower Bio-engineering by CRES-T system
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2. How to use this database --Search by phenotype--
We recorded the morphological phenotypes observed in Arabidopsis by 79 classified terminology. You can find the gene-constructs that induce the preferable phenotype from this classified terminology by simple interface with checkboxes.
Step 1:
Click the button, "Search By Phenotype", to show the list of classified teminology of phenotypes.
Step 2:
1, You can see the 79 classified terminology of phenotypes in the list.
Please check the phenotype(s) that you are interested in.
(e.g.) If you want to see the plants with the phenotype of meristem-less or fused cotyledon, please check the box next to "meristem-less(code #0102)" and "fused cotyledon(code #0105)".
2, Go down to the bottom of the list after checking, then choose "Gene", "ID" or "phenotype" in pull-down menu for sorting the result.
3, Click the button, "Search By Phenotype", to show the result.
This search is done as "OR" searches but not "AND" searches.
Step 3:
The search result is shown as two simple list-views representing the result of "Individual project" and "Bulk project".
(Please click here to see "What are the individual project and the bulk project?")
The result shows all gene-constructs inducing at least one of selected phenotypes by you in each row of the list-view.
In the result of the "Bulk project", the row highlighted with pink means that there is same phenotype observed in the "Individual project" concerning the identified gene.
These list-views of the result have some information as below,
In the result of Individual project
| Gene |
You can find the codes such as "HRxxxx", "CRxxx" and "DRxxxx". These are Gene codes used in this project. Each code indicats each gene-construct introduced into the plants. If you want to see more information about each gene-construct, please click the gene code with hyperlink (go to next step 4)
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| Phenotype |
Phenotype induced by each gene-construct is described. At least one of the phenotypes that you selected is included here. |
| View photos |
You can find "views" with hyperlink, if photo data exists. Click it to show the photos. |
In the result of Bulk project
| ID and No. |
"ID" is a batch code of each experiment. "No." is a serial number of each plant in this project. Please tell us both ID and No., when you ask us for more information. |
| Gene |
If you could find the codes such as "HRxxxx", "CRxxx" and "DRxxxx" with hyperlink in this column, it means that we have identified which gene-construct is introduced into this plant.
If you want to see more information about each gene-construct, please click the gene code with hyperlink (go to next step 4)
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| Phenotype |
Phenotype induced by each gene-construct is described. At least one of the phenotypes that you selected is included here.If there is same phenotype observed in the "Individual project" concerning the identified gene, the row is highlighted into pink. |
| View photos |
You can find "views" with hyperlink, if photo data exists. Click it to show the photos. |
Step 4:
If you click the hyperlink of Gene code in the search result such as "HRxxxx", "CRxxx" and "DRxxxx", you can see the page showing gene information as below.
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The page shows information regarding the status of the "Individual project", and the phenotype recorded by "Individual project" and "Bulk project" with photos, if they exist. It also shows information of floricultural plants, including species, gene constructs and phenotypes induced by them with photos, if they exist. |
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What are the individual project and the bulk project?
*Individual project*
In the individual project, we made multiple transgenic plants by transforming each gene construct one by one.
*Bulk project*
In the bulk project, mixtures of 40 to 60 gene constructs were transformed into Arabidopsis and several hundred transgenic plants were cultivated to identify, in an efficient manner, TFs that induce intriguing phenotypes (Mitsuda et al. 2008; Shikata et al., 2011).
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